• Journal of Yeungnam Medical Science
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• 제32권2호
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• pp.143-145
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• 2015

Page kidney refers to the phenomenon of hypertension secondary to long-standing compression of renal parenchyma caused by renal subcapsular collection. The most common cause of renal subcapsular collection is a hematoma which usually occurs after a history of blunt trauma. A 42-year-old female patient who received botulinum toxin injection in her back during chiropractic care was admitted to the emergency room with sudden bilateral flank pain and hypertension. The computed tomography (CT) images demonstrated the presence of bilateral subcapsular renal hematoma. The patient was treated conservatively and recovered well. The follow up CT images showed markedly resolved bilateral hematoma.

• 한국식품영양학회지
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• 제29권1호
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• pp.33-36
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• 2016

Carbonic anhydrase is essential for the cellular transportation of hydrogen and bicarbonate ions and plays a key role in a wide variety of physiological processes. Rainbow trout, Oncorhynchus mykiss is an important freshwater fish in aquaculture industry and is known to be one of the most susceptible species to environmental contamination. In this study, carbonic anhydrase was detected in the kidney and intestine of rainbow trout. Carbonic anhydrase was isolated from cytosolic proteins and identified by using SDS-PAGE, isoelectric focusing, and immunohistochemical methods. A specific protein band with molecular weight of 30 kDa and pI of 7.0 was detected by Western blotting. The immunohistochemical results showed that carbonic anhydrase was located at various cells in the kidney and intestine of rainbow trout.

• Bulletin of the Korean Chemical Society
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• 제31권9호
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• pp.2493-2496
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• 2010

Erythropoietin (EPO) is mainly produced in kidney and stimulates erythropoiesis. The use of recombinant EPOs for doping is prohibited because of its performance enhancing effect. This study investigated whether biosimilar EPOs could be differentiated from endogenous one by iso-electro-focusing plus double blotting and SDS-PAGE for antidoping analysis. The established method was validated with positive control urine. The band patterns were reproducible and meet the criteria, which was made by world anti doping agency (WADA). Isoelectric focusing was conducted in pH range 2 to 6. Recormon (La Roche), Aropotin (Kunwha), Epokine (CJ Pharm Co.), Eporon (Dong-A), Espogen (LG Life Sciences), and Dynepo (Shire Pharmaceuticals) were detected in basic region. All biosimilars showed discriminative isoelectric profiles from endogenous EPO profiles, but they showed different band patterns with the reference one except Epokine (CJ Pharm Co.). Next, SDS-PAGE of biosimilar EPOs resulted in different molecular weight patterns which were distributed higher than endogenous EPO. Commercial immune assay kit as an immune affinity purification tool and immobilized antibody coated magnetic bead were tested for the purification and concentration of EPO from urinary matrix. The antibody-coated magnetic bead gave better purification yield. The IEF plus double blotting and SDS-PAGE with immunoaffinity purification method established can be used to discriminate biosimilar EPOs from endogenous EPO.

• KSBB Journal
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• 제22권1호
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• pp.53-57
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• 2007

강낭콩 유식물로부터 PBS에 의한 추출, $(NH_4)_2SO_4$ 침전, Sepadex G-100 column chromatography에 의해 lectin을 분리한 다음, 이들의 생화학적 특성으로서 분자량, 적혈구 응집반응, 열 안정성, 최적 온도 및 최적 pH를 연구하였다. 이 과정에 토끼 혈액의 적혈구를 이용하여 활성을 측정하였다. 이 lectin의 분자량은 46 kDa와 44 kDa로서, 각각 2개의 subunit를 갖는 tetramer이다. 정제된 이 lectin의 최적 반응 온도는 30$^{\circ}C$이며, $40\sim80^{\circ}C$에서 열 안정성을 보였다. 또한 이 lectin의 최적 pH는 pH 8.2이다.

• 생명과학회지
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• 제14권2호
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• pp.315-319
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• 2004

사람의 serine palmitoyltransferase(SPT, EC 2.3.1.50)에 대한 항체를 제작하기 위하여 E. coli발현 vector인 pRset vector에 SPTLC1 및 SPTLC2 유전자를 subcloning하고 BL21 (DE3)pLys cell에 발현시켰다. 포유동물의 SPT는 원핵세포의 SPT homodimer와는 달리 SPTLC1 및 SPTLC2 2개의 sub-unit로 된 heterodimer이다. Human embryo kidney cell인 HEK293 cell의 total RNA로부터 RT-PCR을 행하여 cDNA library를 얻은 다음 SPTLC1 및 SPTLC2의 특이적인 primer 들을 이용하여 PCR을 행하였다. SPTLC1 및 SPTLC2 DNA를 hexahistidine fusion 단백질을 발현시킬 수 있는 pRset vector에 cloning하여 pRsetB/SPTLC1 및 pRsetA/SPTLC2를 얻고 염기서열을 확인하였다. 재조합 plasmid를 발현세포인 BL21 cell에 형질전환시킨 다음 ampicillin 및 chroramphenicol 배지에서 선별하여 재조합세포를 얻었다. 1 mM IPTG로서 발현을 유도하였으며 세포 단백질을 SDS-PAGE로 분리한 다음 His-tag antibody로 western blotting을 행하여 SPTLC 및 SPTLC2가 발현되었음을 확인하였다.

• KSBB Journal
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• 제11권3호
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• pp.295-302
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• 1996

Rubisco의 parameter들을 측정하는 것은 광합성 연구에 대단히 중요한데, 본 연구에서는 방사성통위 원소를 사용하는 방법 이 아닌 photometric assay 방 법을 사용하여 parameter를 측정하였다. Rubisco 의 activation과 carbamylation에 미치는 고 CO2 놓도의 효과를 연구하기 위하여, 정상 $CO_2$ 농도 (350 ppm)와 고 $CO_2$ 농도(650 ppm)에서 기른 kidney bean (Phaseolus vulgaris L.) 잎을 각각 재료로 하여, dual beam(334 nm9-} 405 nm) spectro­p photometer를 사용하여 rubisco의 initial activity 와 total activity, carbamylation rate 및 量을 측정 비교하였으며, SDS-PAGE에 의해 rubisco의 전기 영통 profile을 분석하였다. 정상 $CO_2$ 농도에서의 initial activity와 total activity는 $41.2{\mu}M/m^2/s 와 52.2{\mu}M/m^2/s$이며, 고 $CO_2$ 농도에서는 $27.4{\mu}M/m^2/s 와 46.1{\mu}M/m^2/s$로서, 350 ppm에서 650 ppm으로 $CO_2$ 농도를 증가시키면 rubisco의 initial activity와 total activity가 갑소되었다. 또한 carbamylation율도 정상 $CO_2$ 농도에서는 79%이며, 고 $CO_2$ 농도에서는 58.9%로서, $CO_2$ 농도의 증가에 따라 감소되 었다. Rubisco 의 量은 정상 $CO_2$농도에서는 $1.94 {\mu}M/m^2$$ 임에 반해 고 $CO_2$ 농도에서는 $1.58{\mu}M/m^2$ 로서, $CO_2$2 농도는 증가되었는데 그 量은 감소되었다. 이와 같이 고 $CO_2$농도에셔 rubisco의 activity가 감소되는 것은 rubisco의 carbamylation에 기인되는 것으로 생각된다. SOS-PAGE 분석에서 50kO 분자량의 large s subunit와 14.5 kO의 분자량을 가지는 small subu n mt를 동정하였는데, 고 $CO_2$ 농도와 정상 $CO_2$ 농도 의 50kD와 14.5 kO band의 intensity를 비교하면 두 구 사이에 큰 차이가 발견되지 않았다. 고 CO2 농도에서 정상 $CO_2$ 농도로 switch한 rubisco의 parameter는 정상 $CO_2$ 농도에서의 pa­r rameter와 거의 비슷한 값을 나타내였는데, 이는 350 ppm의 정상 $CO_2$ 농도에 의해 rubisco의 acti­v vity가 회복되었음을 의미한다.

• 대한수의학회지
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• 제36권4호
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• pp.809-819
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• 1996

This study was conducted to identify the effects of caffeine or combinations of caffeine and iron or vitamin E on the lipid and protein components in the MDBK(Mardin-Darby Bovine Kidney) cells. For the In vitro test, MDBK cells in ${\alpha}$-MEM(Minimum Essential Medium) were divided into 4 treatment groups according to drug types and dosages as follows; the control(group A), group B was treated with 0.3mM caffeine, group C was treated with 0.3mM caffeine and 0.3mM ferric chloride, group D was treated with 0.3mM caffeine and 0.3mM vitamin E. Those groups were further divided into 5 subgroups according to the time lapsed(control, 4hrs, 8hrs, 24hrs and 48hrs lapsed group). The concentrations of the carbonyl group and malondialdehyde(MDA) and the patterns of the SDS-PAGE(Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) and fatty acid compositions were analyzed to determine the oxidative damages and metabolic changes on the lipid and protein components in the MDBK cells. The results obtained from this study were summarized as follows; 1. The concentrations of carbonyl group and malondialdehyde in MDBK cells of group C were significantly higher(p<0.01) in comparison to the control, and increased according to the time lapsed. But the results of groups B and D were little different in comparison to the group C. 2. As the analytical results of fatty acid compositions in MDBK cells, the proportions of palmitoleic acid and linoleic acid in groups B, C and D were lower in comparison to the control, while the proportion of arachidonic acid in groups B, C and D were significantly higher(p<0.01) in comparison to the control. 3. In order to determine the oxidative damages to the protein in MDBK cells, the patterns of the SDS-PAGE were examined and the patterns of SDS-PAGE in groups C and D were significantly different between 43kd and 200kd of molecular weight.

• 한국식품영양과학회지
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• 제31권1호
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• pp.1-6
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• 2002

강낭콩을 가열한 후 B. subtilis ATCC 51189로 발효시켜 새로운 활성 단백질의 생성여부를 확인하였다. 생강낭콩의 수용성 단백질은 열처리에 의하여 감소하였으며, 발효과정에서 단백질의 양은 변화하지 않았으나, 새로운 아미노산이 합성되었다. 생강낭콩의 수용성 단백질(raw protein, RP)은 분자량 118 kDa의 PHA(phytohemagglutinin)의 전형적인 모습을 보였으나, 열처리한 단백질(heated protein, HP)의 경우, RP의 band는 사라지고, 저분자 peptide로 변화되었으며, 발효과정에 의하여 분자량 18.0 kDa의 새로운 단백질(fermented protein, FP)이 생성된 것을 확인하였다. 또한, RP는 128 HU, HP는 4HU, FP는 32HU의 적혈구응집효과를 보였으며, 위암세포(SNU-1)에 대하여 RP 및 FP는 비교적 높은 농도($IC_{50}$/ $\mu\textrm{g}$/mL)에서 항암활성을 보였다. 그리고, RP 및 FP의 경우 1 $\mu\textrm{g}$/mL에서 림프구 증식효과를 보였고, IFN-${\gamma}$, IL-12의 분비를 촉진하였다. 그러나 HP는 항암효과, 림프구 증식효과, cytokine의 분비촉진효과를 보이지 않았다. 따라서, 강낭콩 lectin은 가열에 의하여 활성물질이 파괴되나, B. subtilis에 의한 발효과정에서 새로운 cytokine 분비촉진물질이 생성되는 것을 확인할 수 있었다.

• BMB Reports
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• 제31권6호
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• pp.542-547
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• 1998

The main function of adipocytes in various species is to store nutrient energy in the form of triglycerides, and this function may he closely related with hormonal signaling through the plasma membrane of adipocytes. Using SDS-PAGE, two-dimensional gel electrophoresis, and a membrane biotinylation technique, we have identified a 55KDa protein (55K protein) from the plasma membrane fraction of adipocytes, with an isoelectric point (pI) of 8.1-8.3. However, this 55K protein was not observed with a two-dimensional gel electrophoresis carried out on plasma membrane fractions prepared from the liver, heart, and kidney tissues. An analysis of the 12 amino acids sequence at the N-terminal of the 55K protein showed that it has a similar sequence to that of bovine serum albumin.

• 한국연초학회지
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• 제23권2호
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• pp.103-108
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• 2001

Hog cholera virus(HCV) was purified from virus infected Bovine kidney cells. From this virus, total protein was analyzed by SDS-PAGE gel electrophoresis and about 55 kDa band of E2 envelope protein was detected. The viral RNA was purified and E2 cDNA was amplified by RT-PCR. E2 cDNA fragment was cloned to PCRII-TOPO cloning vector and named pE2. The analysis of nucleotide sequence showed that this E2 cDNA fragment inserted into pE2 was 1191 nucleotides long and coded 397 amino acids.