Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
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Cloning and Sequence Analysis of Hog Cholera Virus(HCV) E2 Gene

돼지 콜레라 바이러스 E2 유전자의 클로닝 및 염기서열분석

  • 이영기 (한국인삼연초연구원 원료연구부) ;
  • 강신웅 (한국인삼연초연구원 원료연구부) ;
  • 김선원 (한국인삼연초연구원 원료연구부) ;
  • 박성원 (한국인삼연초연구원 원료연구부) ;
  • 이종철 (한국인삼연초연구원 원료연구부) ;
  • 이청호 (한국인삼연초연구원 원료연구부)
  • Published : 2001.12.01
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Abstract

Hog cholera virus(HCV) was purified from virus infected Bovine kidney cells. From this virus, total protein was analyzed by SDS-PAGE gel electrophoresis and about 55 kDa band of E2 envelope protein was detected. The viral RNA was purified and E2 cDNA was amplified by RT-PCR. E2 cDNA fragment was cloned to PCRII-TOPO cloning vector and named pE2. The analysis of nucleotide sequence showed that this E2 cDNA fragment inserted into pE2 was 1191 nucleotides long and coded 397 amino acids.

Keywords

References

  1. Anal. Biochem. v.162 Singlestep method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Chomczynski, P;N. Sacchi
  2. AIDS Res Hum Retroviruses v.7 no.10 Identification of conserved residues in the human immuno deficiency virus type 1 principal neutralizing determinant that are involved in fusion. Freed E.O;R. Risser
  3. Science. v.5 no.268(5211) Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Haq T.A.;H.S. Mason;J.D. Clements;C.J. Arntzen
  4. J Virol v.67 no.9 Glycoprotein E1 of hog cholera virus expressed in insect cells protects swine from hog cholera. Hulst M.M.;D.F. Westra;G. Wensvoort;R.J. Moormann
  5. Arch. Virol. v.140 no.8 Comparison of the entire nucleotide and deduced amino acid sequences of the attenuated hog cholera vaccine strain GPE- and the wild-type parental strain ALD. Ishikawa, K.;H. Nagai;K. Katayama;M. Tsutsui;K. Tanabayashi;K. Takeuchi;M. Hishiyama;A. Saitoh;M. Takagi;K. Gotoh;M. Muramatsu;A. Yamada
  6. Proc Natl Acad Sci U S A v.15;89 no.24 Expression of hepatitis B surface antigen in transgenic plants. Mason H.S.;D.M. Lam;C.J. Arntzen
  7. Biotechnology v.13 no.13 Expression of the rabies virus glycoprotein in transgenic tomatoes. McGarvey P.B.;J. Hammond M. M. Dienelt D.C. Hooper Z. F. Fu;B.Dietzschold;H. Koprowski;F. H. Michaels
  8. Virus Res. v.11 no.4 Hog cholera virus: identification and characterization of the viral RNA and the virus specific RNA synthesized in infected swine kidney cells. Moormann R.J.;M.M. Hulst
  9. Virus Genes v.10 no.2 Baculovirus expression and affinity purification of protein E2 of classical swine fever virus strain Alfort/187 Ruggli, N.;C. Moser;D. Mitchell;M. Hofmann;J.D. Tratschin
  10. Cold Spring Harbor Laboratory Press Molecular cloning : A Laboratory Manual, 2nd Ed. Sambrook, F.;E.F. Fritsch;T. Maniatis
  11. J Virol. v.67 no.12 Processing of pestivirus polyprotein : cleavage site between autoprotease and nucleocapsid protein of classical swine fever virus. Stark R.;G. Meyers;T. Rumenapf;H.J. Thiel