• 대한한의학회지
• /
• 제26권4호
• /
• pp.62-73
• /
• 2005

Objectives: This study was designed to identify the ergogenic effects of Red ginseng and Paeonia radix mixtures and optimal ratios. Materials and Methods: This study was conducted by administering treatments of Red ginseng and Paeonia radix mixtures to rats and by measuring the time to exhaustion by treadmill running. Results: The treatment of Red ginseng and Paeonia radix mixtures to the rats increased the time to exhaustion by treadmill running. The most potent inhibition of Red ginseng and Paeonia radix mixtures on the 5-HT synthesis and the TPH expression in the dorsal raphe was observed at the dose of 200 mg/kg and the optimal ratio of Red ginseng and Paeonia radix for the maximum efficacy was 50:50. Under normal conditions (not exercise), long-term treatment of Red ginseng and Paeonia radix mixtures did not affect the 5-HT synthesis and the TPH expression in the dorsal raphe, suggesting that Red ginseng and Paeonia radix mixtures does not alter serotonin level in the normal rats. The suppressive effect of Red ginseng and Paeonia radix mixtures on the 5-HT synthesis and the TPH expression during exercise is a possible ergogenic mechanism of these mixtures. Conclusions : Red ginseng and Paeonia radix mixtures reduce exercise-induced fatigue, and have the effect of acting as ergogenic aids on the time to exhaustion by treadmill exercise and on 5-HT synthesis and TPH expression.

• Journal of Plant Biotechnology
• /
• 제38권3호
• /
• pp.234-240
• /
• 2011

The genus Paeonia belongs to the family Paeoniaceae having significant medicinal and ornamental importance. The present investigation was undertaken with an aim to understand phylogenetic relationships of three Paeonia species (P. lactiflora, P. obovata, and P. suffruticosa) that are widely distributed in China, Korea, and Japan, using nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequence and to compare the phylogeny results with investigations reported earlier using existed sequences of the same species. The size variation obtained among sequenced nrDNA ITS region was narrow and ranged from 722 to 726 bp. The highest interspecific genetic distance (GD) was found between P. lactiflora and P. suffruticosa or P. obovata. The phylogram obtained using our nrDNA ITS sequences showed non-congruence with previous hypothesis of the phylogeny between section Paeonia and section Moutan of genus Paeonia. This result was supported by the phylogenetic relations showed in the phylogram constructed with existed sequences in NCBI. The present study suggested that P. obovata belonging to section Paeonia was phylogenetically closer to P. suffruticosa representing section Moutan of genus Paeonia than P. lactiflora belonging to section Paeonia. The main reason of the paraphyly of section Paeonia is thought to be nucleotide additivity directly caused by origin hybridization. This study provides more sequence sources of genus Paeonia, and will help for further studies in intraspecies population, and their phylogentic analysis and molecular evolution.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.195-200
• /
• 2003

The growth of S. aureus was inhibited by combination of natural products bearing antioxidative capacity and combined two, three and four kinds of them. S. aureus was controlled by Paeonia Japonica, Galla Rhois, Geranii Herba, alone, and two mixed combinations of Paeonia japonica and Scutellaria Baicalensis George, Paeonia japonica and Theae Folium, Paeonia japonica and Chelidonium Majus L, Paeonia japonica and Geranii Herba, Paeonia japonica and Schizandrae Fructus, and three mixed combinations of Paeonia Japonica and Scutellaria Baicalensis George and Geranii Herba, Paeonia Japonica and Scutellaria Baicalensis George and Chelidonium Majus L., Paeonia Japonica and Scutellaria Baicalensis George and Theae Folium, and four mixed combinations of Scutellaria Baicalensis George and Chelidonium Majus L. and Theae Folium and Schizandrae Fructus, Paeonia Japonica and Scutellaria Baicalensis George and Chelidonium Majus L. and Schizandrae Fructus, Scutellaria Baicalensis George and Geranii Herba and Theae Folium and Schizandrae Fructus. As these combinations of natural products will activate some parts of body, they may be applied to pharmaceutical applications, functional foods, antiaging tea, also expected to control bacterial growth for fermentative beverage bearing multifunction.

• Applied Biological Chemistry
• /
• 제45권3호
• /
• pp.173-178
• /
• 2002

약용식물의 생산량을 증가시키고 지상부 꽃은 관상용으로 활용할 수 있는 연구의 일환으로 목단 줄기에 작약 뿌리를 접목시켜 생육생태를 관찰하고 유효지표성분외에 무기성분, 지방산, 유기산등 성분변화 및 향미 관능검사등을 비교한 결과 생육생태를 볼 때 지상부는 목단줄기에 목단꽃이 피었으며 지하부 뿌리는 작약뿌리와 목단뿌리가 혼재하여 생성되었으며, 작약뿌리와 목단뿌리의 혼재비율은 약 3:1정도로 나타났다. paeoniflorin및 paeonol 분석결과 건조시킨 작약근의 함량은 대건물 시료(g)중 9.91 mg/g및 0.80 mg/g,접목 작약근은 11.71 mg/g 및 0.35 mg/g, 목단피는 10.81 mg/g 및 2.84 mg/g, 접목 목단피는11.26 mg/g 및 3.28 mg/g으로 유사한 경향이었다. 무기성분별 10종 함량은 대체로 유사한 경향이었으나 Fe함량은 시료별로 차이가 있었으며 작약근은 86.06 mg/g, 접목 작약근 127.36 mg/g, 목단피 75.05 mg/g, 접목 목단피는 140.63 mg/g으로 함량 차이가 현저하였다. 지방산은 linoleic acid, palmitic acid, linolenic acid 및 oleic acid등이 주성분으로 총 11종을, 유기산은 oxalic acid, citric acid 및 malic acid등이 주성분으로 총 6종을 각각 분석하였으나 함량 조성은 대체로 유사한 경향이었다. 한편, 향미 관능평가 결과 작약근과 접목 작약근은 대체로 유사한 향미 패턴을 나타내었으나, 작약근은 작약근 향미 및 떫은맛이 다소 강하였으며, 목단피와 접목 목단피도 거의 유사한 향미를 나타내었으나, 목단피가 목단피 향미, 쓴 맛, 화한 맛 및 떫은 맛이 다소 강하였다.

• 생약학회지
• /
• 제41권1호
• /
• pp.6-8
• /
• 2010

Crude drug 'SanJagYag' has been used mainly as a pleurisy, abdominal pain, female disease and gastropathy. To clarify the botanical origin of SanJagYag from Korea, the anatomical characteristics of Paeonia japonica, Paeonia lactiflora and Paeonia obovata of Paeoniaceae were studied. As a result, it was clarified that SanJagYag was the root of Paeonia obovata.

• 한국약용작물학회지
• /
• 제8권4호
• /
• pp.297-303
• /
• 2000

Paeonia lactiflora Pallas와 Paeonia obovata Maxim.의 뿌리와 줄기를 식물 해부학적으로 살펴본 결과 도관절의 길이에 있어서는 P. lactiflora Pallas가 P. oboata Maxim. 보다 줄기 및 뿌리에서 다소 길게 나타났으며 도관절의 폭에 있어서도 P. lactiflora Pallas가 P. oboata Maxim.의 줄기와 뿌리에 비해 넓게 분화된 양상을 관찰 할 수 있었다. P. lactiflora Pallas의 뿌리에서 이기목부(二期木部)는 불연속적인 층상배열을 이루나 P. oboata Maxim.에서는 연속적인 방사열을 이루어 종간의 뚜렷한 차이를 나타냈다.

• 한국작물학회지
• /
• 제37권3호
• /
• pp.283-287
• /
• 1992

작약의 번식방법과 작약근 / 목단접수의 유효성분을 비교 조사한 결과를 요약하면 다음과 같다. 1. 작약실생묘 파종적기는 수확후 같은해 8월20일에서 9월10일에 파종하는 것이 발아율이 양호하였다. 2. 분주법은 춘식보다 추식이 작약의 생육에 유리하였다. 3. 삽목법은 개화중에 실시하는 것이 발근율이 양호하였고, 생장조정제는 무처리관행 대비보다 Ruton 처리가 발근율이 21% 증가하였으나 정아출현이 되지 않았다. 4. 작약근 / 목단접수의 접목시기는 9월이 적기이고, 작약근 / 목단접수에서 뿌리 생산성도 가장 우수하였다. 5. 작약 유효성분은 paeoniflorin 성분이 가장 많았고, 목단은 paeonol 성분이 많았으며, 작약근 / 목단접수의 paeoniflorin 성분은 작약보다 75.9% 높았으며, 기타 유효성분 또한 작약근과 비슷한 경향이었다.

• Advances in Traditional Medicine
• /
• 제7권5호
• /
• pp.540-548
• /
• 2008

Paeonia radix is the root of Paeonia aliflora Pallas, which is a perennial plant classified in the family Paeoniaceae. Paeonia radix possesses several pharmacological effects such as analgesic, anti-inflammatory and anti-allergic, anti-oxidative, and anti-coagulant activities. In this study, we investigated the effect of the aqueous extract of Paeonia radix on the lipopolysaccharide-induced inflammation in mouse BV2 microglial cells. The aqueous extract of Paeonia radix at respective concentration was treated one hour before lipopolysaccharide treatment. In the present results, the aqueous extract of Paeonia radix suppressed prostaglandin $E_2$ synthesis and nitric oxide production by inhibiting the lipopolysaccharide-stimulated mRNA expressions of cyclooxygenase-2 and inducible nitric oxide synthase in mouse BV2 microglial cells. These results demonstrate that Paeonia radix exerts anti-inflammatory and analgesic effects probably by suppressing mRNA expressions of cyclooxygenase-2 and inducible nitric oxide synthesis. The present study demonstrates that Paeonia radix may offer a valuable mean of therapy for brain inflammatory diseases.

• Journal of Acupuncture Research
• /
• 제17권1호
• /
• pp.221-250
• /
• 2000

In order to investigate experimentally that the effect of aqua-acupuncture solution of Paeonia lactiflora on arthritis of mice induced by collagenII, the author performed several experimental items : that is increase, paw thickness, DTH, weight of spleen, hematological change, expression of $CD4^+$, $CD8^+$, $CD19^+$, gene expression of IL-1, IL-6, IL-12, IFN-${\gamma}$, TNF, proliferation of synovial cells and cytotoxicity. The results were obtained as follows; 1. Inhibitory effects of aqua-acupuncture solution of Paeonia lactiflora on arthritis induced by collgenII. 1) In incidence, paw edema, AI and DTH were inhibited as compared with control group. 2) The splenic weight was increased and the number of leukocytes was decreased as compared with control group. 3) The number of $CD4^+$, $CD8^+$ activated cells and surface-receptor expression were increased as compared with control group. 4) In hematological change, total protein, creatinine and LDH were decreased significantiy as compared with control group. 2. FACS analysis on normal BABL/c of spenic cells treated with aqua-acupuncture solution of Paeonia lactiflora. 1) Aqua-acupuncture solution of Paeonia lactiflora activated adhesive splenic cells of mice morphologically in vitro. 2) Aqua-acupuncture solution of Paeonia dactiflora enhanced the gene expression of IL-12 and also enhanced that of interferon-${\gamma}$ remarkably. 3) Aqua-acupuncture solution of Paeonia lactiflora reduced the number of $CD4^+$, $CD8^+$, $CD19^+$ activated cells and their surface-receptor expression as compared with control group. 3. Effects of aqua-acupuncture solution of Paeonia lactiflora on human synovial cells. 1) In cytotoxicity against synovial cells, aqua-acupuncture solution of Paeonia lactiflora didn't show cytotoxicity at concentration of $10-100{\mu}g/m{\ell}$ but showed significantly at concentration of $200-400{\mu}g/m{\ell}$ as compared with control group. 2) Aqua-acupuncture solution of Paeonia lactiflora reduced the gene expression of IL-6, IL-$1{\beta}$ and TNF-${\alpha}$. 3) Aqua-acupuncture solution of Paeonia lactiflora inhibited proliferation of synovial cells at concentration of 100 and $200{\mu}g/m{\ell}$.

• 한국토양비료학회지
• /
• 제24권3호
• /
• pp.206-209
• /
• 1991

농가에서 3년간 재배중인 작약(芍藥), 목단(牧丹), 모단(牡丹)을 접붙인 작약(芍藥)(목단(牧丹)/작약(芍藥))근(根)을 수집해서 외형적(外形的) 생육특성(生育特性)과 유효성분(有效成分)을 분석한 결과 다음과 같은 결론(結論)을 얻었다. 1. 목단(牧丹)/작약(芍藥)을 접목(接木)한 지하부(地下部)의 외형적 생육상태(生育狀態)는 작약(芍藥)의 특성과 같았으나, 달관(達觀) 조사(調査)에 의하면 목단(牧丹)/작약접목근(芍藥接木根)이 작약(芍藥)에 비해 2~3배의 증수량(增收量)을 나타냈다. 2. 목단속(牧丹屬)(Paeonia linne) 식물의 주요(主要) 유효성분(有效成分)인 Paeoniflorin은 목단(牧丹)/작약근(芍藥根)이 0.98%로 작약(芍藥) 0.78%, 목단(牧丹) 0.46%보다 0.2%에서 0.5%까지 각각 높게 나타났으며, 목단(牧丹)에 다량 함유된 Paeonol은 목단접목(牧丹接木)으로 인해 목단(牧丹)/작약(芍藥) 접목근(接木根)에서 함량(含量)이 증가되지는 않았다. 3. 결과적(結果的)으로 수량(收量)과 유효성분(有效成分) 측면을 종합비교(綜合比較)해 볼 때, 작약(芍藥)의 뿌리를 대목(臺木)으로 하여 모단경(牡丹莖)을 접수(接穗)로 접목(接木)함으로서 근생육(根生育)이 괄목할만하게 증대되었고, 약효성분(藥效成分)은 작약(芍藥) 고유의 함량비(含量比)를 유지하는 것으로 추정되어 향후 체계적인 연구의 필요성을 제시해 주었다.