• Journal of Microbiology
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• 제34권4호
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• pp.320-326
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• 1996

To analyze the effects of host versus plasmid on survival of 2, 4-degrading bacteria in environmental samples, strains Pseudomonas cepacia/pJP4, Alcaligenes JMP228/pJP4, P. cepacia/p712, and Alcaligenes JMP228/p712 were separately inoculated into samples of field soil, paddy soil, lake water, and river water, and then the changes of their populations were measured. The strains used contained a 2, 4-D degradative plasmid, either pJP4 conferring fast-growing property to the host or p712 conferring slow-growing property, and were resistant to antibiotics such that the inoculated strains could be enumerated against the indigenous microbial populations. In sterile environmental samples, these strains were stably maintained at the levels used for inoculation, except in sterile paddy soil where Alcaligenes JMP228 strains died drapidly. In natural soil samples for four strains declined steadily with time, but in naturla water samples their polulations fell rapidly at the early phase and then remained almost constant. When the environmentla samples were treated with 2, 4-D, P. cepacia/pJP4 and P. cepacia/p712 maintained significant numbers, while Alcaligenes JMP228/pJP4 and Alcaligenes JMP228/p712 declined significantly in most of the samples. The results indicated that the survivability of genetically modified microorganisms could vary depending on the environments and that their abundance in the environments under s2, 4-D selection was markedly influenced by the nature of the 2, 4-D degradative plasmid as well as type of the host strain.

• 한국농공학회논문집
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• 제60권1호
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• pp.79-87
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• 2018

Water management impacts both methane ($CH_4$) and nitrous oxide ($N_2O$) emissions from rice paddy fields. Although irrigation is one of the most important methods for reducing $CH_4$ emission in rice production systems it can also $N_2O$ emissions and reduce crop yields. A feasibility study on the system of rice intensification (SRI) methods with respect to irrigation requirements, greenhouse gas (GHG) emissions was conducted for either 2 or 3 years depending on the treatment in Korea. The SRI methods (i.e. SRI and midsummer drainage (MD) with conventional practice (CT)) reduced the irrigation requirement by 49.0 and 22.0 %, respectively. Global warming contribution of GHG to different depending on the type of GHG. Therefore, the emission of $CH_4$ and $N_2O$ shall be converted to Global Warming Potential (GWP). The GHG emission from the conventional practice with midsummer drainage (MD) and the SRI plots, in GWP were reduced by 49.1 and 77.1 %, respectively. Application of SRI water management method could help to improve Korea's water resources and could thus contribute to mitigation of the negative effects of global warming.

• Journal of Microbiology and Biotechnology
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• 제30권11호
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• pp.1688-1696
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• 2020

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH₄⁺-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.

• Journal of Biosystems Engineering
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• 제38권1호
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• pp.1-8
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• 2013

Purpose: Path planning and tracking algorithms applicable to various agricultural operations, such as tillage, planting, and spraying, are needed to generate steering angles for auto-guidance tractors to track a point ahead on the path. An optimal coverage path algorithm can enable a vehicle to effectively travel across a field by following a sequence of parallel paths with fixed spacing. This study proposes a path generation and tracking algorithm for an auto-guided Korean tractor with a tillage implement that generates a path with C-type turns and follows the generated path in a paddy field. A mathematical model was developed to generate a waypoint path for a tractor in a field. This waypoint path generation model was based on minimum tractor turning radius, waypoint intervals and LBOs (Limit of Boundary Offsets). At each location, the steering angle was calculated by comparing the waypoint angle and heading angle of the tractor. A path following program was developed with Labview-CVI to automatically read the waypoints and generate steering angles for the tractor to proceed to the next waypoint. A feasibility test of the developed program for real-time path tracking was performed with a mobile platform traveling on flat ground. The test results showed that the developed algorithm generated the desired path and steering angles with acceptable accuracy.

• 대한지리학회지
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• 제36권3호
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• pp.258-277
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• 2001

농협 연쇄점을 정보화로 인한 商的 유통의 합리화와 재고 집약형 대규모 물류센터의 다극화로 물적 유통 의 집약화가 이루어져 유통의 효율성을 높였고, 수송도 외부수주로 이루어지고 있다. 이에 따라 생활용품과 농산물의 배송방식은 각각 루트배송과 정시 일괄배송으로 상품의 특성에 따라 그 배송권이 형성되었다. 한편 상품 판매활동에 의한 공간적 특성은 답작농업의 발달과 서비스업 취업 정도에 따라 판매 상품류의 구성이 결정된다는 점이 밝혀졌다. 그러나 일상생왈 상품인 가공 식품류와 잡화류는 농협 연쇄점이 입지한 지역유형에 영향을 받지 않고 판매된다는 점이 규명되었다.

• 상하수도학회지
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• 제28권3호
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• pp.265-276
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• 2014

In this study, we suggested 4 plans to reduce non-point pollutant sources in Dongcheon and analyzed their controlling effects by water quality modeling, XP-SWMM. To do this we identified the influx of non-point pollutant sources to the initial rainwater through the water quality survey in the river and analyzed the causes of them at major locations, and suggested 4 kinds of plans reducing non-point pollutant sources. Plans reducing the non-point pollutant sources through cleaning the industrial road around the river(plan A), through a separate treatment facilities like the gutter(plan B), through installing treatement facilities(plan C), or through combing plan B and C(plan D) were analyzed using XP-SWMM model. The analysis showed that plan A, B, C and D reduced non-point pollutant sources average 21.7 %, 24.7 %, 49.3 %, 56.7 % respectively. Therefore, the water quality pollution in Dongcheon due to the influx of non-point pollutant sources is considered to be reduced effectively though cleaning the road, installed at the exits of paddy or factory basins, invasion type facilities or equipment-type facilities.

• 한국농공학회논문집
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• 제60권6호
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• pp.133-144
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• 2018

To suggest the field maintenance plan considering the geographical characteristics of the region, we selected representative regions(plain regione and mountain region) and compared spatial distribution of cultivated land in Muan-gun and Hwasun-gun. Firstly, we examined the distribution characteristics of cultivated land according to the scope of the maintenance object with Fragstats. As a result of that, it was found that the cultivated area except rice paddy had the highest aggregation effect. And then, we developed type classification of maintenance considering geographic characteristics and cultivated crops information. As a result of classification, plain land type Muan region was mostly cultivated land suitable for integrated maintenance. On the other hand, Hwasun, a mountainous terrain, needs small-scale maintenance and road maintenance. Based on these results, it was found that more detailed planning is needed for the upland field infrastructure improvement considering the topographic characteristics.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.152-152
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• 2017

Plants exposed to environmental stress for long durations often can adapt to stress conditions with improved tolerance. Moreover this acquired tolerance to stress can be retained even after reverting to destressed growth conditions, which is known to stress memory. In these adaptation and stress memory processes, epigenetic regulation, such as DNA methylation and histone modifications play a key role. Here, we showed that regenerated rice plants from embryogenic callus exposed to gradually increasing NaCl concentrations (up to 120 mM NaCl) acquired salt tolerance and their enhanced tolerance are inherited to subsequent generations. The rice plants (R0) regenerated from rice callus under saline conditions were transplanted into normal paddy field and R1 seeds were harvested. These R1 seeds displayed higher germination rate on MS medium containing 100mM NaCl than wild-type. The callus derived from R1 seeds showed better growth than control callus on high salinity medium. And the salt-adapted R1 plants exhibited higher chlorophyll contents and also higher $K^+/Na^+$ ratio than wild-type rice under saline conditions. The results indicated that rice plants successfully adapted to saline growth conditions during regeneration on high salt medium and moreover this acquired tolerance to salt stress was inherited subsequent generation.

• Journal of Biosystems Engineering
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• 제12권3호
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• pp.42-49
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• 1987

The threshing action of the head-fed type threshing unit occurs mainly by the impact between threshing tooth and grains. It may be therefore the most fundamental step to calculate the time and order of the occurrance of impact by the tooth for predicting the performance of threshing unit. The threshing teeth arrangement was defined by length and diameter of threshing dram, number of spiral arrays, number of threshing teeth by kind per one spiral array, number of windings of spiral array around the threshing drum, delay angle of impact line. The linear equations for locus of left and right margin of paddy bundle, spiral array, impact line on the development figure of the threshing drum were expressed by fastors of the threshing teeth arrangement. In the computer program, the teeth which inflict impact were searched successively along the impact line. Searching range and impact condition were defined by the relation between four linear equations. If the impacting tooth was found, time and the kind of threshing tooth was derived from the coordinate of the threshing tooth. At this time the unit torque curve was accumulated on the array of computer memory. At last the completed torque curve of threshing drum shaft was described on the computer screen. Remarkably the peack valae and fluctuation of torque curve was decreased by adopting the delay angle of impact line.

• Journal of Biosystems Engineering
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• 제25권3호
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• pp.227-232
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• 2000

A natural air in-bin grain dryer with a grain circulator was developed for on farm use. Natural air drying test for rough rice was carried out to evaluate drying rate, uniformity of moisture content distribution in grain bed and energy consumption. It took 10 days to dry 8 ton of paddy rice from 21.9%(w.b) of moisture contents to 16.7%(w.b), by prototype dryer and the average drying rate was 0.52%/day. The uniformity of moisture content after drying was superior to a conventional natural air dryer in which grains were not mixed during drying periods. The dryer performance evaluation index was 738.3kJ/(kg.water), which was more effective than that of grain circulation type hot air dryer(3,500∼5,000 kJ/kg.water)