• Title/Summary/Keyword: Packed-bed bioreactor

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Removal of ammonia by packed bed bioreactor using immobilized nitrifiers (고정화 생물 반응기에 의한 암모니아 제거)

  • 김병진;이경범;서근학
    • Journal of Environmental Science International
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    • v.8 no.2
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    • pp.177-182
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    • 1999
  • Nitrifier consortium entrapped in Ca-alginate bead were packed into aerated packed bed bioreactor and non aerated packed bed bioreactor and the performances of two bioreactors were evaluated for the removal of ammonia nitrogen from synthetic aquaculture water. Total ammonia nitrogen(TAN) removal rate was decrease in aerated packed bed bioreactor below 0.3hr of hydraulic residence time(HRT), but increased in non aerated packed bed bioreactor until 0.5hr of HRT. At HRT of 0.05hr, TAN removal rate of non aerated packed bed bioreactor was about 335g TAN/㎥/day and the optimum ratio of packing height and inside diameter of reactor (H/D) was 4. The performance of two bioreactors indicated that non aerated packed bed bioreactor was better than aerated packed bed bioreactor in ammonia removal from synthetic aquaculture water.

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A Study on the Kinetics and the Biogas Formation for Organic Wastewater Treatment in Anaerobic Fluidized-Bed Bioreactor and New Model AFPBBR (혐기성 유동층 생물 반응기와 새로운 모델의 AFPBBR에서 유기성폐수 처리시 Biogas 생성과 반응상수에 관한 연구)

  • 김재우;장인용
    • Journal of Environmental Health Sciences
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    • v.19 no.2
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    • pp.23-33
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    • 1993
  • The anaerobic digestion of organic synthetic wastewater in anaerobic fluidized bed bioreactor (AFBBR) and anaerobic fluidized packed bed bioreactor (AFPBBR) was studied. This study was conducted to evaluate efficiency and reliability of two reactor. Experiment was performed to find the effect of upflow rate with AFBBR and the height of packed bed with AFPBBR. As a result, this program obtained several conclusion. These are given as follows: As applied the upflow rate increased in AFBBR the produced volume of biogas increased, while the gas production and COD removal decreased at above 0.3 m$^3$/h. When a upflow rate is 0.4 m$^3$/h in AFBBR the volatile suspended solid (VSS) became significantly increased. At an organic loading rate from 0.1 to 0.4 of upflow rate in AFBBR, the methane yield was 1.5584 m$^3$CH$_4$/kgCOD removed, and the observed cell yield coefficient was 0.0933 gVSS/gCOD. In case of AFPBBR, the results showed also that 20 cm of height of packed bed was superior to other in the aspect ot biogas production, the content of methane and COD removal. At 20 cm of height, the profile of microorganisms was stable, while at 30 cm the VSS of effluent became higher than AFBBR. Though COD removal of AFPBBR increased with packed bed, COD removal deteriorate with over packing because the loss of pressure became higher in the reactor. At an organic loading rate from 20 to 40 cm of packed bed in-AFPBBR, the methane yield was 2.5649 m$^3$CH$_4$/kgCOD removed, and the observed cell yield coefficient was 0.0506 gVSS/gCOD. Based upon the results obtained, it is suggested that AFBBR and AFPBBR is the most effective conditions at 0.3 m3/h of upflow rate, the 20cm of packed bed, respectively. The rate constant are summarized as follow:

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The Characteristics of Attached Biomass and Biogas Production in an Anaerobic Packed Bed Bioreactor with Several Carriers (혐기성 고정상 생물반응기에서 담체의 종류에 따른 Biogas 생성 및 미생물 부착 특성에 관한 연구)

  • 안재동;강동수;장인용
    • Journal of Environmental Health Sciences
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    • v.18 no.2
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    • pp.75-81
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    • 1992
  • An experimental investigation has been carried out to evaluate the characteristics in wastewater treatment using an anaerobic packed bed bioreactor with ceramics, rubber sponge, soft stone A, and soft stone B as carrier. The results of the work have shown that soft stone A as a carrier was superior to other carriers in methane production, then the content of methane for soft stone A was about 70%. First of all, soft stone A had higher efficiency of the COD removal than the others in response of passing the operation, as well as it had a low volatile acid in reactor. In addition, the slope of methane production with respect to the removal of COD ($m^{3}CH_{4}$/kgCOD) was 0.58 for soft stone A. In biomass hold-up equation for each carriers, the equation of soft stone A was m$_{p}$=714 ($C_{o}/0.41+C_{o}$) and it was the largest in this experimented carriers. Based upon the results obtained, it is suggested that the major effective carrier in wastewater treatments within the packed bed bioreactor used in this experimental work by soft stone A.

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Production of High Fructose Syrup by Flocculated Actinoplanes missouriensis KCTC 1780 (Actinoplanes missouriensis KCTC 1780의 응집 균체에 의한 과당생산)

  • 조정일
    • The Korean Journal of Food And Nutrition
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    • v.6 no.4
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    • pp.268-275
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    • 1993
  • The whole cells of Actinoplanes missouriensis KCTC 1780 which produce glucose isomerase was immobillized by flocculation method for the effective production of high fructose syrup using packed-bed bioreactor system. Among the flocculation methods used In this study, the glucose Isomerase activity of flocculated cells using 5% polyethylenimmine and 0.2% glutaraldehyde was the highest as 46.3 unit, and the flocculant was 10.3g(wet weight) per 100m1 of broth, and the residual activity was 92.5%. In the batch operation of glucose isomerization using the flocculated cells, the optimum pH, temperature and isomerization ratio were 7.0, 75$^{\circ}C$ and 31%, respectively. The optimum concentration of Mg2+ which was activator on the glucose isomerization of flocculated cells was 0.1M, and glucose isomerase activity was increased by about 40% compared to none of Mg2+. In the packed-bed bioreactor system with 1.2 hour of residence time at 7$0^{\circ}C$, the reaction stability maintained until 96 hour without toss of activity, and the equilibrium was kept up to 120 hours of the operation.

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Bioreactor Cultures of Lithospermum erythrorhizon for Shikonin Production with In Situ Extraction (동시 추출을 겸한 생물반응기에서 Lithospermum erythrorhizon 배양에 의한 shikonin 생산)

  • 김동진;장호남
    • Microbiology and Biotechnology Letters
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    • v.18 no.5
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    • pp.525-529
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    • 1990
  • Plant cell cultures of Lithospermum erythrorhizon were performed in stirred tank and packed-bed reactors with in situ extraction by n-hexadecane. The specific shikonin production and volumetric shikonin productivity of stirred tank reactor reached 1.5 mg shikoninlg cell and 400$\mu g$ shikonin/(L.day), respectively. In packed-bed reactor with calcium alginate-immobilized cells specific shikonin production and volumetric productivity reached 2.0 mg shikoninlg cell and 2857$\mu g$ shikonin/(L.day), which were 1.3 and 7.1 times higher than those of stirred tank reactor, respectively. The higher shikonin production and productivity of packed-bed reactor seemed to be due to high cell loading capacity of calcium alginate immobilized cells in packed-bed reactor and improved cell-cell contact.

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Biofuel Production by Immobilized Living Cells - Hydrogen Production by Photosynthetic Bacteria - (고정화 미생물에 의한 에너지 생산 - 광합성 박테리아에 의한 수소 생산 -)

  • 조영일;선용호
    • Microbiology and Biotechnology Letters
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    • v.13 no.3
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    • pp.303-309
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    • 1985
  • Continuous production of hydrogen by Ca alginate-immobilized photosynthetic bacteria was studied in a packed-bed bioreactor. The dilution rate and input concentration of carbonaces substrate were selected as operating parameters. To choose the strain for immobilization, hydrogen productivities of Rhodopseudomonas caposulata 10006 and Rhodospirillum rubrum KS-301 were compared through preliminary batch cultures of their free cells: the former was found to show better hydrogen productivity in spite of its lower specific growth rate. For the continuous production of hydrogen by immobilized R capsulata, the optimum dilution rate was about 0.84 h$^{-1}$ . The Immobilized tells gave better hydrogen yield and conversion efficiency than free ones. And a kinetic parameter K'$_{m}$ was determined for the packed-bed bioreactor, being practically constant for a specific range of dilution rates.s.

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Removal of Low Concentration Ammonia Nitrogen using a Packed Bed Bioreactor Immobilized with Nitrifier Consortium (질화세균을 고정화한 충전층 생물반응기에서 저농도 암모니아성 질소 제거)

  • Lee, Chang-Keun;Kim, Byong-Jin;Lee, Min-Su;Kim, Yong-Ha;Suh, Kuen-Hack
    • Clean Technology
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    • v.13 no.1 s.36
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    • pp.16-21
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    • 2007
  • This study estimated the effect of hydraulic residence time(HRT), influent total ammonia nitrogen(TAN) concentration, temperature and pH in the packed-bed bioreactor using immobilized nitrifiers. Removal rate of ammonia nitrogen was increased with decreasing HRT and the optimum HRT was 0.2 hour when influent TAN was $2g/m^3$. At this point, removal rate was $226.1\;g/m^3{\cdot}day$ and removal efficiency was 88.8%. Removal rate of ammonia nitrogen was Increased with increasing TAN concentration. Removal rate and efficiency of ammonia nitrogen were kept constant at $20{\sim}35^{\circ}C$ and pH $8{\sim}9$ value.

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Continuous Production of Transglucosylated Steviosides Using Immobilized Cyclodextrin Glucanotransferase (고정화 Cyclodextrin Glucanotransferase에 의한 당전이 스테비오사이드의 연속생산)

  • In, Man-Jin;Chae, Hee-Jeong;Kim, Min-Hong
    • Korean Journal of Food Science and Technology
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    • v.29 no.5
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    • pp.969-973
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    • 1997
  • In order to produce transglucosylated steviosides continuously, some types of bioreactors were investigated with cyclodextrin glucanotransferase immobilized on a high porous anion exchange resin, Diaion HPA75. Among the bioreactors, the packed-bed reactor (PBR) showed the highest specific productivity. The effect of linear velocity in a PBR on the stevioside conversion was not significant in the range of $10{\sim}60\;cm/hr$ at the same space velocity of $1.2\;hr^(-1)$. When the space velocity of bioreactor was varied from 0.2 to $1.1\;hr^{-1}$, the optimal velocity of substrate solution was determined as $0.7\;hr^(-1)$. The stevioside conversion of more than 70% was maintained during 20 days in the continuous operation, if about 20% of immobilized enzyme was replaced in the top of reactor after two weeks operation as the one of the control methods in bioreactor. The specific production, which refers to as the amount of commercially valuable transglucosylated steviosides produced by a unit amount of immobilized cyclodextrin glucanotransferase, was found to be ca. 150g product/g immobilized enzyme.

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Optimization of Tannase Production by Aspergillus niger in Solid-State Packed-Bed Bioreactor

  • Rodriguez-Duran, Luis V.;Contreras-Esquivel, Juan C.;Rodriguez, Raul;Prado-Barragan, L. Arely;Aguilar, Cristobal N.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.9
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    • pp.960-967
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    • 2011
  • Tannin acyl hydrolase, also known as tannase, is an enzyme with important applications in the food, feed, pharmaceutical, and chemical industries. However, despite a growing interest in the catalytic properties of tannase, its practical use is very limited owing to high production costs. Several studies have already demonstrated the advantages of solid-state fermentation (SSF) for the production of fungal tannase, yet the optimal conditions for enzyme production strongly depend on the microbial strain utilized. Therefore, the aim of this study was to improve the tannase production by a locally isolated A. niger strain in an SSF system. The SSF was carried out in packed-bed bioreactors using polyurethane foam as an inert support impregnated with defined culture media. The process parameters influencing the enzyme production were identified using a Plackett-Burman design, where the substrate concentration, initial pH, and incubation temperature were determined as the most significant. These parameters were then further optimized using a Box-Behnken design. The maximum tannase production was obtained with a high tannic acid concentration (50 g/l), relatively low incubation temperature ($30^{\circ}C$), and unique low initial pH (4.0). The statistical strategy aided in increasing the enzyme activity nearly 1.97-fold, from 4,030 to 7,955 U/l. Consequently, these findings can lead to the development of a fermentation system that is able to produce large amounts of tannase in economical, compact, and scalable reactors.

Trends in Monoclonal Antibody Production Using Various Bioreactor Systems

  • Jyothilekshmi, I.;Jayaprakash, N.S.
    • Journal of Microbiology and Biotechnology
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    • v.31 no.3
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    • pp.349-357
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    • 2021
  • Monoclonal antibodies are widely used as diagnostic reagents and for therapeutic purposes, and their demand is increasing extensively. To produce these proteins in sufficient quantities for commercial use, it is necessary to raise the output by scaling up the production processes. This review describes recent trends in high-density cell culture systems established for monoclonal antibody production that are excellent methods to scale up from the lab-scale cell culture. Among the reactors, hollow fiber bioreactors contribute to a major part of high-density cell culture as they can provide a tremendous amount of surface area in a small volume for cell growth. As an alternative to hollow fiber reactors, a novel disposable bioreactor has been developed, which consists of a polymer-based supermacroporous material, cryogel, as a matrix for cell growth. Packed bed systems and disposable wave bioreactors have also been introduced for high cell density culture. These developments in high-density cell culture systems have led to the monoclonal antibody production in an economically favourable manner and made monoclonal antibodies one of the dominant therapeutic and diagnostic proteins in biopharmaceutical industry.