The Korean Journal of Food And Nutrition (한국식품영양학회지)
- Volume 6 Issue 4
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- Pages.268-275
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- 1993
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- 1225-4339(pISSN)
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- 2287-4992(eISSN)
Production of High Fructose Syrup by Flocculated Actinoplanes missouriensis KCTC 1780
Actinoplanes missouriensis KCTC 1780의 응집 균체에 의한 과당생산
Abstract
The whole cells of Actinoplanes missouriensis KCTC 1780 which produce glucose isomerase was immobillized by flocculation method for the effective production of high fructose syrup using packed-bed bioreactor system. Among the flocculation methods used In this study, the glucose Isomerase activity of flocculated cells using 5% polyethylenimmine and 0.2% glutaraldehyde was the highest as 46.3 unit, and the flocculant was 10.3g(wet weight) per 100m1 of broth, and the residual activity was 92.5%. In the batch operation of glucose isomerization using the flocculated cells, the optimum pH, temperature and isomerization ratio were 7.0, 75
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