• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.4
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• pp.344-348
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• 2019

This study assessed the microbiological hazards of gloves worn during the shell shucking process of the oyster Crassostrea gigas, and we suggest an in situ method for minimizing microbial contamination. The study consisted of two groups, one in which the working gloves were periodically replaced (PRG) with new gloves, and another in which the gloves were not replaced (NRG). In the PRG group, gloves were replaced every 2 h during 8 h of processing. Food pathogenic bacteria Escherichia coli, Salmonella species, and Listeria monocytogenes were not found in any samples, including gloves and shucked oysters. However, Staphylococcus aureus (SA) was detected in some samples, and the contamination levels were correlated with the working time and the regular replacement of gloves. SA was not detected on gloves or oysters of the PRG group. However, it was detected in the range of <$15CFU/15cm^2$ to $2.9{\times}10^2CFU/15cm^2$ on gloves after 6 h of continuous work, and from <$15CFU/15cm^2$ to $2.23{\times}10^2CFU/15cm^2$ on oysters after 8 h. These results indicate that the SA contamination in shucked oysters originated from the working gloves, and that replacement of working gloves every 2-4 h will minimize SA contamination in oyster products.

• Development and Reproduction
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• v.25 no.1
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• pp.67-72
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• 2021

This study investigated the IGF-1 signal in specific tissues using Pacific oysters artificially matured via water temperature elevation. Pacific oysters were subjected to water temperature elevation from March to June, and 20 were randomly sampled each month. The condition index (CI) and tissue weight rate (TWR) were examined by measuring shell length, shell height, shell width, and soft tissue weight. The IGF-1 signal in tissues (adductor muscle, digestive glands, gills, labial palps, mantle edges, and gonads) was analyzed by sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. From April to June, the TWR of females and males increased from 19.1±2.9 to 21.0±3.6 and 18.2±2.0 to 19.2±2.5, respectively, while the CI remained the same. The IGF-1 signal in each tissue differed. IGF-1 was expressed in the adductor muscle, while tyrosine was expressed in all tissues. The phosphor (p)-ERK and p-AKT activities were high in the adductor muscle, mantle edge, and gonads. IGF-1 signaling affected the growth and maturity of the Pacific oysters examined.

• Journal of the korean society of oceanography
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• v.33 no.3
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• pp.90-99
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• 1998

Butyltin and phenyltin residues were quantified in seawater and biota of the Chinhae Bay System, Korea in 1995. Butyltin compounds were detected in all seawater and biota samples, whereas phenyltin compounds were found only in the biota samples. Tributyltin (TBT) concentrations in seawater ranged from < 8-35 ng Sn/l. Tributyltin concentrations in Crassostyea gigas and Thais clavigera ranged from 95-885 and 23-414 ng Sn/g, respectively, Triphenyltin(TPhT) concentrations in each species ranged 155-678 and 46-785 ng Sn/g, respectively. Spatial distribution of TBT was closely related to boating and dry-docking activities. However, spatial distribution of TPhT was not consistent with that of TBT. The biological concentration factor for TBT in C. gigas was about 25000 that is four times greater than that of T. clavigera. Butyl- to phenyltin concentration ratio was greater than one in C. gigas, but that in T. clavigera was less than one. Major tissues of C. gigas also showed different accumulation patterns for butyl- and phenyltin compounds. Furthermore, 19 and 28% of total body burdens of TBT and TPhT were found in gonadal mass of C. gigas just prior to spawning.

• Ocean and Polar Research
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• v.34 no.2
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• pp.151-163
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• 2012

Digestive cells of the bivalves have a highly developed lysosomal system and the system is known to be sensitive to changes in environmental qualities. In this study, we measured lysosomal responses of the digestive cells in wild oyster, Crassostrea gigas using frozen section. Oysters were collected in June 2010 from intertidal areas in the inner and outer bay of Gwangyang off the south coast of Korea. From the tissue sections, we measured the digestive cell lysosomal membrane stability (LMS), level of neutral lipids (NL), lipofuscin (LF) and the digestive gland atrophy (DGA). The DGA and condition index of oysters from the inner bay were significantly lower (P<0.05). The statistical test indicated that LMS levels of oysters in the inner bay and the outer bay were not significantly different since a shorter activity was displayed by oysters from the inner bay than that of oysters in outer bay. The LF deposition level of the oysters in the inner bay displayed significantly higher levels than the outer bay (P<0.05). In contrast, the NL accumulation measured from oysters in outer bay was significantly higher than the level observed in the inner bay (P<0.05). Different levels of DGA and LF that were observed in the inner and outer bays were thought to be associated to different level of environmental contamination and these two assays are considered to be good biomarkers.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.21-28
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• 2012

The combined effects of water temperature and salinity on the settlement rate and density (spats/$cm^2$) of Crassostrea gigas pediveliger larvae were studied under the hatchery conditions. Four water temperatures (20.0, 23.0, 26.0 and $29.0^{\circ}C$) were tested at three salinities (20.0, 30.0 and 40.0). The optimum water temperature by salinity conditions were $29.0^{\circ}C$ and 20.0, 30.0 which significantly (P < 0.05) the highest mean (${\pm}S.D$) larval settlement rate of $43.1{\pm}0.1%$, $42.1{\pm}0.1%$, respectively. But the combination of water temperatures and salinities for settlement density was not effected, but each factor as water temperature ($29.0^{\circ}C$), salinity (40.0) was shown significantly high and low settlement density, respectively (P < 0.05). The statistics result of settlement density frequency was appeared: mode ($1.2-2.3spats/cm^2$), Mean ($1.9-2.4spats/cm^2$), Range ($3.0-5.8spats/cm^2$) and the lowest variance was obtained 0.4 at water temperature $20.0^{\circ}C$ by salinity 30.0, but $29.0^{\circ}C$ by 20.0 was the highest (1.1). The best condition at this results for the larvae setting under the artificial seed collection of C. gigas was $29.0^{\circ}C$ and 20.0-30.0.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.512-519
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• 2023

Pacific oysters Crassostrea gigas are a popular shellfish in the Republic of Korea. However, due to their abundant moisture and nutrient content, oysters are susceptible to microbiological growth and biochemical changes, which lead to quality degradation. Therefore, the present study aimed to investigate the effectiveness of modified atmosphere packaging (MAP) in maintaining the quality of raw oysters during storage. Microbiological and physicochemical parameters such as pH, glycogen content, soluble protein, turbidity, and volatile basic nitrogen (VBN) were analyzed for oysters stored under various gas compositions and storage periods. The results showed that there was no significant increase in viable cell count in MAP oysters after six days in MAP oysters. Moreover, the physicochemical quality of non-MAP oysters deteriorated rapidly, whereas the quality of MAP oysters were maintained during storage. This study suggests that MAP can be an effective technique for maintaining the freshness of raw oysters during distribution and storage, and may also be useful for extending the shelf-life and maintaining the quality of other seafood products.

• The Korean Journal of Malacology
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• v.32 no.4
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• pp.269-277
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• 2016

We examined the seasonal variation of the proximate, amino acid, fatty acid composition and glycogen contents of triploid Pacific oyster cultured in western coastal waters of Korea in 2015. The specimens were collected in February, May, August and November from off-bottom farming site. The lipid content was the highest in winter season (February, $2.10{\pm}0.06%$), the protein content was the highest in autumn season (November, $9.09{\pm}0.04%$) while the moisture and ash content was the highest in summer season (August, $86.00{\pm}0.12%$ and $2.67{\pm}0.03%$, respectively). The total amino acid contents was the highest in winter season (1,011.5 mg / 100 g), decreased gradually and was the lowest in summer season (486.7 mg / 100 g). The total amino acid composition was dominated by taurine, aspartic acid, glutamic acid, glycine and alanine. In the fatty acid composition, the two fatty acids, eicosapentaenoic acid and docosahexaenoic acid, which comprised that as much as 50% of the total fatty acid contents, were dominated during all season. The glycogen content was the highest in summer season ($1.26{\pm}0.03g$ / 100 g) although it was stable during the other season with the range of $0.69{\pm}0.04-0.86{\pm}0.01g$ / 100 g. Taken together, these results suggest that triploid pacific oysters have high commercial value not only in winter season but in summer season because they do not spawn and have high contents of glycogen comparing with those of diploid oyster in this period.

• The Korean Journal of Malacology
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• v.26 no.2
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• pp.107-114
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• 2010

To evaluate the culture conditions in oyster-farming waters, chemical and biological measurements were made in seawater and oysters from six bays around Tongyeong in November and December 2003. Nutrient levels in the seawater were higher in the western area than in the eastern area, in contrast to particulate organic matter and dissolved oxygen levels. The mean total polycyclic aromatic hydrocarbon ({\sum}PAH) content of the oysters was 194.5-375.9 ng/g dry weight, with four-ring compounds constituting 34.1%-79.6% of PAH. Despite wide temporal variations, a "western > eastern" spatial distribution of PAH was apparent. These low concentrations of PAHs indicate that Tongyeong waters are pristine in terms of PAH contamination. Among the hemocytic biomarkers, only lysosomal activity was significantly reduced in Hansan-Goje Bay, but did not correlate closely with PAH content. This finding indicates that the impact of PAH on cultured oysters is negligible around Tongyeong waters.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.2
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• pp.210-216
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• 1998

Relationship between population group properties and docosahexaenoic acid (DHA) content of the Pacific oyster had been carried out at the Bukman oyster farm near Tongyeong based on the regular monthly sampling from November 1994 through April 1996. DHA content in lipids was least abundant in June and most abundant in November. Minimum DHA content close to 'zero' in lune suggests that much of DHA are being used for maturing and discharging of eggs and sperms, in consideration of the fact that lure is the spawning periods of the oyster. The corelationships among DHA content, mortality, and growth coefficient, have not been recognized. The approximate positive relations have been acknowledged between DHA content and the individual density in times of harvest, and also the individual weight. But it seems that the relaltions between DHA content and the individual weight are not directly related, rather it seems that it is the result from the population density effects caused by the relations between DHA and the number of individuls. But the meaning of the above mentioned relations can not be clearly defined yet.

• Fisheries and Aquatic Sciences
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• v.16 no.2
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• pp.63-69
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• 2013

Shucked oysters were soaked in an equal weight of salt water and stored at $3^{\circ}C$ for 7 days. Changes in the free amino acid content of the whole body and in the adductor muscle were evaluated by a practical distribution method. With the exception of aspartic acid and tyrosine, no significant changes in free amino acids or ammonia were observed in whole-body shucked oysters during the storage period. In contrast, the majority of free amino acids in the adductor muscle decreased significantly. Most of these free amino acids were detected in considerable amounts in the surrounding salt water after 7 days of storage. Both the weight of the whole body and the salinity of the surrounding salt water decreased significantly during the storage period. These results suggest that free amino acids were eluted from the cutting surface of the adductor muscle and indicate that the free amino acid content per shucked oyster and in the adductor muscle, decreases during cold storage.