• Title/Summary/Keyword: PacC

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Draft Genome Sequence of Aureobasidium pullulans Strain MHAU2101, a Biological Control Agent against Fire Blight from Korea

  • Lin He;Huan Luo;Mi-Hyun Lee;Jun Myoung Yu
    • Microbiology and Biotechnology Letters
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    • v.51 no.4
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    • pp.538-541
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    • 2023
  • In this study, we present the draft genome of Aureobasidium pullulans strain MHAU2101, which is the first strain to effectively control fire blight caused by Erwinia amylovora in Korea. The genome of strain MHAU2101 was composed of 28,669,322 base pairs, with a C+G content of 50.4%. The assembly comprised 17 contigs and had 99.22% completeness. The results of this study will be a valuable resource for future research on the biocontrol mechanism of A. pullulans strain MHAU2101.

Whole Genome Sequence of Streptomyces sp. from Novel Marine Actinomycetes

  • Hyeon Kyeong Lee;Heung-Soon Park;Eung-Soo Kim;Si-Sun Choi
    • Microbiology and Biotechnology Letters
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    • v.51 no.3
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    • pp.325-327
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    • 2023
  • This paper presents the complete genome sequence of a novel marine actinomycete, Streptomyces sp. MMBL 11-1. The genome of Streptomyces sp. MMBL 11-1 was obtained through next-generation sequencing using the PacBio Sequel system and Illumina platform provided by Macrogen, Korea. The assembled genome consists of five contigs, with a total length of 8,496,900 bp and a G+C content of 71.6%. The genome harbors multiple biosynthetic gene clusters (BGCs) associated with producing microbial natural products (MNPs). The comprehensive genomic information of this type of strain will serve as a valuable resource for identifying other marine actinomycetes strains.

Complete genome sequencing and comparative genomic analysis of Lactobacillus acidophilus C5 as a potential canine probiotics

  • Son, Seungwoo;Lee, Raham;Park, Seung-Moon;Lee, Sung Ho;Lee, Hak-Kyo;Kim, Yangseon;Shin, Donghyun
    • Journal of Animal Science and Technology
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    • v.63 no.6
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    • pp.1411-1422
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    • 2021
  • Lactobacillus acidophilus is a gram-positive, microaerophilic, and acidophilic bacterial species. L. acidophilus strains in the gastrointestinal tracts of humans and other animals have been profiled, but strains found in the canine gut have not been studied yet. Our study helps in understanding the genetic features of the L. acidophilus C5 strain found in the canine gut, determining its adaptive features evolved to survive in the canine gut environment, and in elucidating its probiotic functions. To examine the canine L. acidophilus C5 genome, we isolated the C5 strain from a Korean dog and sequenced it using PacBio SMRT sequencing technology. A comparative genomic approach was used to assess genetic relationships between C5 and six other strains and study the distinguishing features related to different hosts. We found that most genes in the C5 strain were related to carbohydrate transport and metabolism. The pan-genome of seven L. acidophilus strains contained 2,254 gene families, and the core genome contained 1,726 gene families. The phylogenetic tree of the core genes in the canine L. acidophilus C5 strain was very close to that of two strains (DSM20079 and NCFM) from humans. We identified 30 evolutionarily accelerated genes in the L. acidophilus C5 strain in the ratio of non-synonymous to synonymous substitutions (dN/dS) analysis. Five of these thirty genes were associated with carbohydrate transport and metabolism. This study provides insights into genetic features and adaptations of the L. acidophilus C5 strain to survive the canine intestinal environment. It also suggests that the evolution of the L. acidophilus genome is closely related to the host's evolutionary adaptation process.

Effect of the Residual Impurity on the Prepreg Surface on the Wettability of Encapsulant for Chip on Board Package (칩 온 보드 패키지 적용을 위한 프리프레그 표면 잔류 불순물이 봉지재의 젖음성에 미치는 영향)

  • Gahui Kim;Doheon Kim;Kirak Son;Young-Bae Park
    • Journal of the Microelectronics and Packaging Society
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    • v.31 no.2
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    • pp.9-15
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    • 2024
  • The effect of the residual impurity on the prepreg surface on the wettability of encapsulant for chip on board package was analyzed with microstructure, compositions and chemical bonds using a scanning electron microscope and X-ray photoelectron spectroscopy. As a result, the contact angle of w/ residual impurity sample was measured to be 28° higher than that of w/o residual impurity sample, and the C-O bond was decreased to be 4% lower than that of w/o residual impurity sample. The surface energy of the prepreg decreased because the impurity ions, Na and F, generated by the manufacturing process and wet etching, reacted chemically with the C on the prepreg surface, forming C-F bonds and breaking the C-O bonds on the prepreg surface. Therefore, the wettability of the encapsulant was degraded because the contact angle between the encapsulant and the prepreg was increased.

A Study on Acoustic Emission Characteristics through the Cyclic Thermal Test of Thermal Barrier Coating by Plasma Spray Process (플라즈마 용사법에 의한 열차폐 코팅의 열피로에 따른 AE신호 특성 연구)

  • Park J.H.;Lee K.H.;Ye K.H.;Kim S.T.;Jeon C.H.;Kim J.S.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.1349-1352
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    • 2005
  • This paper is to investigate a defect for thermal barrier coating layers by acoustic emission method in 4-point bending test. The two-layer thermal barrier coating is composed of $150\mu{m}\;CoNiCrAlY\;bond\;coating\;by\;vacuum\;plasma\;spray(VPS)\;process\;and\;250\mu{m}\;ZrO_2-8wt%Y_2O_3$ ceramic coating layer by air plasma spray(APS) process on Inconel-718. The specimen prepared by cyclic thermal test(500, 1000, 2000cycle) at $1050^{\circ}C$ The AE monitoring system is composed of PICO type sensor, a wide band pre-amplifier(40dB), PC and AE DSP(16/32 PAC) board. The AE event, amplitude, Cumulative energy and count of coating specimens is evaluated according to cyclic thermal test.

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Evaluation of vaccination against Mycoplasma hyopneumoniae in pigs at different vaccination time-points (돼지 마이코플라즈마 폐렴 예방백신의 접종시기별 방어효과에 관한 연구)

  • Lee, Ham-Hee;Rha, James;Han, Jeong-Hee
    • Korean Journal of Veterinary Service
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    • v.31 no.3
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    • pp.291-303
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    • 2008
  • This study was performed to evaluate the effect of commercial swine mycoplasma vaccine (M+$Pac^{(R)}$, Schering-Plough Animal HealthCo.) at different vaccination time points. Total 139 piglets were moved to experimental farm after weaning and were randomly allocated to 3 treatment groups and 1 control group. Piglets of 3 treatment groups (A, B and C) were vaccinated twice at 1 and 3 weeks (A group), 3 and 5 weeks (B), and 6 and 8 weeks (C), respectively. All vaccinates showed higher antibody titers compared with nonvaccinates, and maintained to finish (P<0.001). Performance and RBC/WBC count showed no significant difference between groups. Vaccinates at 6-8 weeks showed better effect on decrease of clinical sign compared with vaccinates at 1-3 weeks and 3-5 weeks and nonvaccinates. Also, lung lesion assessment showed significant difference between vaccinates at 3-5 weeks and 6-8 weeks, and nonvaccinates and vaccinates at 1-3 weeks (P<0.05). Vaccinates at 3-5 score in comparison with other groups. In conclusion, these results suggested that vaccination at 3-5 weeks or later may be more effective than earlier vaccination.

Membrane Concentrate Thickening by Hollow-fiber Microfilter in Drinkin Water Treatment Processes

  • 이병호
    • Membrane Journal
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    • v.1 no.1
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    • pp.100-100
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    • 1991
  • A novel system to thicken the concentrated colloidal solution from membrane water treat-ment processes was developed. A hollow-fiber microfilter(hydrophilic polyethylene nominal pore size 0.1 μm total surface area 0.42 m2) was installed in an acrylic housing that has an aeration port 5 cm below the membrane and a clarifier in the bottom. The concentrate was uniformly supplied from the top of the housing. Bacuum filtration caused downward flow of concentrate and as a result thickening interface. The addition of poly-aluminum chloride (PAC) resulted in rapid increase of trans-membrane pressure (TMP) and in no improvement of the filtered water turbidity and thickening process. Two types of con-centrate and concentrate turbidity had little effect on the increase of TMP and concentrate thickening. It was observed that for the same height of membrane housing membrane surface area to housing volume (A/V) ratio had significant effect on the increase of TMP. When the housing volume was increased ten times the increasing rate of TMP was three times faster as compared to the original housing. A hydraulic model successfully simulated the formation and sedimentation of thickening interface.

DC Characteristics of AlGaN/GaN HFETs Using the Modeling of Piezoelectric and Thermal Effects (Piezoelectric효과와 열 효과 모델링을 고려한 AlGaN/GaN HFET의 DC 특성)

  • Park, Seung-Wook;Hwang, Woong-Joon;Shin, Moo-Whan
    • Korean Journal of Materials Research
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    • v.13 no.12
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    • pp.769-774
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    • 2003
  • In this paper, we report on the DC characteristics of the AlGaN/GaN HFETs using physical models on piezoelectric and thermal effects. Employing the models was found to be essential for a realistic prediction of the DC current-voltage characteristics of the AlGaN/GaN HFETs. Though use of the implementation of the physical models, peak drain current, transconductance, pinch-off voltage, and most importantly, the negative slope in the current were accurately predicted. The importance of the heat sink effect was demonstrated by the comparison of the DC characteristics of AlGaN/GaN HFETs fabricated from different substrates including sapphire, Si and SiC. Highest peak current was achieved from the device with SiC by an effective suppression of heat sink effect.

Complete genome sequence of Acidovorax citrulli strain KACC17005, a causal agent for bacterial fruit blotch on watermelon (수박에 과실썩음병을 일으키는 Acidovorax citrulli strain KACC17005의 유전체 해독)

  • Park, Hye-Jee;Seong, Hoon Je;Sul, Woo Jun;Oh, Chang-Sik;Han, Sang-Wook
    • Korean Journal of Microbiology
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    • v.53 no.4
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    • pp.340-341
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    • 2017
  • Acidovorax citrulli is a causal agent for bacterial fruit blotch on watermelon. Here, we report the complete genome sequence of A. citrulli strain KACC17005. The genome contains 5,349,924 bp with G + C contents of 68.54%, including 4,520 protein coding genes in a circular chromosome. It also possesses at least 15 genes encoding putative type III effector proteins, which may contribute to promoting virulence in susceptible hosts or triggering immune responses in resistant hosts.

PSNR Evaluation of P Company DSA System between Server Display Monitor and Client Display Monitor (P사 DSA 시스템의 Server Display Monitor와 Client Display Monitor의 PSNR 평가)

  • Lee, Junhaeng
    • Journal of the Korean Society of Radiology
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    • v.8 no.1
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    • pp.43-49
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    • 2014
  • PACS is needed medical imaging with large-capacity storage device. Slower transmission degrades the performance of the PACS. Thus, the image read by the reading of the long-term stored image without compromising the quality of the video, which does not affect future readings in the range will be compressed and stored. Compression and video storage, and video transport Noise generated during storage and transmission of medical images and the resulting loss of information that occurs when the monitor output from many problems. The study estimates server display monitor and client display monitor of philips DSA system, and suggests that the evaluation and improvement about PSNR, process from server display signal obtaining to client display monitor. P company DSA is used in the test. Two monitors that are $1280{\times}1024$ pixel monitor of P company and 1536x2048 pixel monitor of Wide are used displaying angiography picture. MARO-view is taken in PACS program, and Visual $C^{++}$ is taken as accomplishing PSNR measurement program. As a result of experiment, no change in No 1, 3 of PSNR appear that there is no error in telephotograph and display. In terms of compressibility, low compressibility has small change of definition, and there was not remarkable drawback of compressibility which has little change in definition.