• Mass Spectrometry Letters
• /
• 제3권2호
• /
• pp.39-42
• /
• 2012

Phosphorylation and glycosylation are two of the most important and widespread post-translational modifications (PTMs) in an organism. Proteomics analysis of the PTMs has been challenged by low stoichiometry of the modified proteins and suppression effects by high abundance proteins, typically no-functional house-keeping proteins. In this study, a novel method was applied for not only isolating PTM peptides from intact peptides but also concurrently characterizing of glyco- and phosphoproteome using electrostatic repulsion hydrophilic interaction chromatography (ERLIC) packed with silica coated by crosslinked polyethyleneimine. For 2 mg tryptic digest of mouse proteome of epicardial adipose tissue with fat diet, 802 N-glycosylated peptides of 316 glycoproteins and 159 phosphorylated peptides of 75 phosphoproteins were identified using HPLC chip/quadrupole time-of-flight (Q-OF) tandem mass spectrometer.

• Journal of Computing Science and Engineering
• /
• 제11권4호
• /
• pp.111-120
• /
• 2017

Post-translational modifications (PTMs) of proteins play substantial roles in the gene regulation of cell physiological functions and in the generation of major diseases. However, the majority of existing studies only explored a certain PTM of proteins, while very few have investigated the PTMs of two or more domains and the effects of their interactions. In this study, after collecting data regarding a large number of breast cancer-related and validated PTMs, a sequence and domain analysis of breast cancer proteins was carried out using bioinformatics methods. Then, protein-protein interaction network-related tools were applied in order to determine the crosstalks between the PTMs of the proteins. Finally, statistical and functional analyses were conducted to identify more modification sites of domains and proteins that may interact with at least two or more PTMs. In addition to exploring the associations between the interactive effects of PTMs, the present study also provides important information that would allow biologists to further explore the regulatory pathways of biological functions and related diseases.

• 대한치과교정학회지
• /
• 제17권1호
• /
• pp.63-72
• /
• 1987

This is to investigate the difference between craniofacial correlation of Angle's class III malocclusion and that of normal occlusion. For this investigation, 28 adult males and famales, who have class III malocclusion, and 35 adult males and 46 adult famals., who have normal occlusion were selected to measure actual dimension of cranial base and to investigate ratio of various parts of maxillofacial skeleton against cranial base. Class III malocclusion were, also , classified based on SNA and SNB angle in normal range. Results were as follows. 1. In class III malocclusion, actual dimension of cranial base were appeared to be less than normal occlusion in both sex. 2. In class III malocclusion, values were less than normal occlusion in BN/ptm-A in both sex, but had no significance between two. 3. In class III malocclusion, ramal inclination, mandible inclination, BN/Go-Pog, lower genial angle were appeared to be greater. Thus, characteristic mandibular protrusive appearance of class III malocclusion was due to relative ratio and morphologic characteristic of mandibular body dimension against cranial base. 4. In class III malocclusion, upper incisors were labially tilted and lower incisors were lingually tilted compared to normal occlusion. 5. In typing of class III malocclusion, the most common type was found to be one which maxillas were in normal range, while mandibles were in protrusive tendency.

• Mass Spectrometry Letters
• /
• 제9권2호
• /
• pp.51-55
• /
• 2018

Capture of non-glycoproteins during lectin affinity chromatography is frequently observed, although it would seem to be anomalous. In actuality, lectin affinity chromatography works at post-translational modification (PTM) sites on a glycoprotein which is not involved in protein-protein interactions (PPIs). In this study, serial affinity column set (SACS) using lectins followed by proteomics methods was used to identify PPI mechanisms of captured proteins in human plasma. MetaCore, STRING, Ingenuity Pathway Analysis (IPA), and IntAct were individually used to elucidate the interactions of the identified abundant proteins and to obtain the corresponding interaction maps. The abundant non-glycoproteins were captured with the binding to the selected glycoproteins. Therefore, depletion process in sample pretreatment for abundant protein removal should be considered with more caution because it may lose precious disease-related low abundant proteins through PPIs of the removed abundant proteins in human plasma during the depletion process in biomarker discovery. Glycoproteins bearing specific glycans are frequently associated with cancer and can be specifically isolated by lectin affinity chromatography. Therefore, SACS using Lycopersicon esculentum lectin (LEL) can also be used to study disease interactomes.

• Mass Spectrometry Letters
• /
• 제11권3호
• /
• pp.52-58
• /
• 2020

Histidine phosphorylation (pHis) is increasingly recognized as an important post translational modification (PTM) in regulating cellular functions in eukaryotes. In order to clarify the role of pHis in mammalian cell signaling system, a global phosphorylation study was performed in human prostate cancer cells, PC-3M, using a TiO₂ affinity chromatography. A total number of 307 pHis sites were identified on the 268 proteins among total identified 9,924 phosphorylation sites on 3,316 proteins. In addition, 22 pHis proteins were classified in enzyme category. This report provides the first database for the study of pHis in prostate cancer cells.

• Journal of Biosystems Engineering
• /
• 제12권3호
• /
• pp.7-16
• /
• 1987

This study was carried out to develop a microcomputer-based data acquisition and control system which was able to collect the data of engine performance test automatically and control the speed and load of the engine. The results of the study are summarized as follows: 1. The signal processing devices, which were able to measure cylinder pressure, coolant temperature, compositions of exhaust gas, fuel consumption, engine rpm and torque etc., were developed. The results of calibration showed that all of devices had high accuracy ranging from 0.3% to 0.69% respectively. 2. The PIA (peripheral interface adapter) for interfacing digital signal and PTM (programmable timer module) for displaying real time every 0.0408 sec were designed and developed. 3. An engine-speed control system using a stepping motor and driver was developed. The control system had the stability, and faster settling time than the manual control system. 4. The automatic control system of electrical dynamometer, which was able to control the speed and load of dynamometer, was developed with a SSD (shackleton system driver) and D/A converter. 5. The computer programs, which were able to collect and process the data of engine tests, were developed using both the machine language and BASIC.

• 치과방사선
• /
• 제25권2호
• /
• pp.459-470
• /
• 1995

The purpose of this study was to determine whether any difference existed in craniofacial morphology between cleft children and normal subjects. Thirty three measurements of the various regions of cranium and face were obtained from lateral cephalometric radiograms in 40 cleft children(27 males, 13 females) and 40 normal subjects(23 males, 17 females) in our dental hospital from Jan. 1988 to Dec. 1995.The measurements were compared with those in control subjects who had no history of craniofacial abnormalities. The obtained results were as follows: 1. In the cranium, the cleft children had singificantly shorter posterior cranial base length(S-Ba) and total antero-posterior cranial base length(N-Ba) (P<0.05). 2. In the upper face, the cleft children had significantly shorter upper anterior facial height(N-ANS) and upper posterior facial height(Ptm'-SNL) (P<0.05). 3. In the lower face, the cleft children had significantly shorter antero-posterior mandibular length(Pog-Ar) and antero-posterior mandibular body length(Pog-Go) (P<0.05). 4. In the facial profile, the cleft children had significantly shorter total facial height(N-Me} and posterior facial height(S-Go) (P<0.05).

• 대한치과교정학회지
• /
• 제18권1호
• /
• pp.235-252
• /
• 1988

The quadrilateral analysis is a proportional analysis which evaluates the skeletal configuration of lower face on the relations between both jaws in the horizontal as we]1 as vortical dimensions. This study was undertaken to analyse the harmony and disharmony of quadrilateral patterns in normal occlusion and malocclusion. The present study was carried out on lateral cephalograms of 530 Korean children; the subjects consisted of 135 normal occlusions (63 male and 72 female), 105 Class II division 1 malocclusions (52 male and 53 female), 109 Class III malocclusions (50 male and 59 female), 91 hypodivergent facial types (44 male and 47 female) and 90 hyperdivergent facial types (45 male and 45 female). The following conclusions were reached: 1. Means and standard deviation in each group and sex were obtained from normal occlusion and malocclusion. 2. Quadrilateral mean diagram in normal occlusion was constructed for male and female, respectively. 3. In normal occlusion, 1:1 ratio exists between the maxillary base length (A' to Ptm') and mandibular base length (B' to J'), but lower facial height is targer than above. 4. Difference is effective to estimate the degrees of Class II and Class III malocclusion, and lower facial height (LFH) and sagittal angle is effective to recognize the hypodivergent and hyperdivergent facial type. 5. Quadrilateral analysis is able to visualize the anteroposterior and vertical dysplasia of lower face, and it is helpful to recognize certain problems in malocclusion.

• Mass Spectrometry Letters
• /
• 제15권1호
• /
• pp.1-25
• /
• 2024

Protein glycosylation, a highly significant and ubiquitous post-translational modification (PTM) in eukaryotic cells, has attracted considerable research interest due to its pivotal role in a wide array of essential biological processes. Conducting a comprehensive analysis of glycoproteins is imperative for understanding glycoprotein bio-functions and identifying glycosylated biomarkers. However, the complexity and heterogeneity of glycan structures, coupled with the low abundance and poor ionization efficiencies of glycopeptides have all contributed to making the analysis and subsequent identification of glycans and glycopeptides much more challenging than any other biopolymers. Nevertheless, the significant advancements in enrichment techniques, chromatographic separation, and mass spectrometric methodologies represent promising avenues for mitigating these challenges. Numerous substrates and multifunctional materials are being designed for glycopeptide enrichment, proving valuable in glycomics and glycoproteomics. Mass spectrometry (MS) is pivotal for probing protein glycosylation, offering sensitivity and structural insight into glycopeptides and glycans. Additionally, enhanced MS-based glycopeptide characterization employs various separation techniques like liquid chromatography, capillary electrophoresis, and ion mobility. In this review, we highlight recent advances in enrichment methods and MS-based separation techniques for analyzing different types of protein glycosylation. This review also discusses various approaches employed for glycan release that facilitate the investigation of the glycosylation sites of the identified glycoproteins. Furthermore, numerous bioinformatics tools aiding in accurately characterizing glycan and glycopeptides are covered.

• 폴리머
• /
• 제39권3호
• /
• pp.499-505
• /
• 2015

Dimethyldimethoxysilane(DMDMS), methyltrimethoxysilane(MTMS)과 phenyltrimethoxysilane(PTMS)을 이용하여 폴리유기실록산 열 경화성 코팅액을 제조하고 가교도와 페닐기 함량이 코팅액과 도막 물성에 미치는 영향을 살펴보았다. Si-NMR 측정에서 얻어진 D $T^{Me}$ $T^{Ph}$ 구조 코팅액 조성비가 사용된 DMDMS, MTMS, PTMS의 몰 비로부터 얻어진 이론치와 일치함을 확인할 수 있었다. 가교밀도가 높을수록 그리고 페닐 함량이 낮을수록 실록산 결합의 증가에 의하여 코팅액 분자량이 증가됨을 알 수 있으며 페닐기 증가는 코팅액 굴절률 증가에 매우 큰 영향을 미침을 알 수 있었다. 가교밀도가 높을수록, 페닐기 함량이 감소할수록 코팅도막 경도는 증가되었으며 내열특성 또한 우수해짐을 확인할 수 있었다. 이상의 결과로부터 같은 실록산 결합을 갖는 폴리유기실록산 코팅제라도 치환도에 따른 가교도 조절과 페닐기 함량에 따라 코팅액 물성이 달라짐을 확인할 수 있었다.