• Journal of the Korean Society of Food Science and Nutrition
• /
• v.39 no.9
• /
• pp.1328-1334
• /
• 2010

Low-trans spread fat (LTSF) was produced by lipase-catalyzed synthesis of canola (CO) and fully hydrogenated soybean oil (FHSBO) at 65:35 (w/w). Blend of CO and FHSBO with 65:35 ratio was interesterified using Lipozyme TLIM (immobilized Thermomyces lanuginosus, 20% of total substrate) in a 1 L-batch type reactor at $70^{\circ}C$ with 500 rpm for 24 hr. Then, physicochemical melting properties of LTSF were compared with commercial spread fat. At $20^{\circ}C$, solid fat contents (SFC) of commercial spread fat as a control and LTSF were similar, showing 19.1 and 18.1%, respectively. Major compositional fatty acids of LTSF were C18:0, C18:1 and C18:2 (29.2, 41.8 and 13.3 wt%, respectively). Trans fatty acid content of the LTSF (0.2 wt%) was lower than that of commercial spread fat (5.5 wt%). In the RP-HPLC analysis from LTSF, major triacylglycerol (TAG) molecules were SOL (stearoyl-oleoyl-linoleyl), SOO, POS/PSP, and SOS. Also, polymorphic form and x-ray diffraction of LTSF showed coexistence of $\beta$' and $\beta$ form crystals.

• Physical Therapy Korea
• /
• v.11 no.4
• /
• pp.7-17
• /
• 2004

Individuals who propel wheelchairs have a high prevalence of upper extremity injuries (i.e., carpal tunnel syndrome, elbow/shoulder tendonitis, impingement syndrome). Musculoskeletal injuries can result from overuse or incorrect use of manual wheelchairs, and can hinder rehabilitation efforts. To better understand the mechanisms of upper extremity injuries, this study investigates the motion of the wrist during wheelchair propulsion. This study also examines changes in the variables that occur with fatiguing wheelchair propulsion to determine how the time parameters of wheelchair propulsion and the state of fatigue influence the risk of injury. A two dimensional (2-D) analysis of wrist movement during the wheelchair stroke was performed. Twenty subjects propelled a wheelchair handrim on a motor-driven treadmill at two different velocities (50, 70 m/min). The results of this study were as follows; The difference in time parameters of wheelchair propulsion (cadence, cycle time, push time, recovery time, and PSP ratio) at two different velocities was statistically significant. The wrist kinematic characteristics had statistically significant differences at two different velocities, but wrist radial deviation and elbow flexion/extension had no statistically significant differences. There were statistically significant differences in relation to fatigue in the time parameter of wheelchair propulsion (70 m/min) between initial 1 minute and final 1 minute. The wrist kinematic characteristics between the initial 1 minute and final 1 minute in relation to fatigue had statistically significant differences but the wrist flexion-extension (50 m/min) had no statistically significant differences. According to the results, the risk of musculoskeletal injuries is increased by fatigue from wheelchair propulsion. To prevent musculoskeletal injuries, wheelchair users should train in a muscle endurance program and consider wearing a splinting/grove. Moreover, wheelchair users need education on propulsion posture, suitable joint position, and proper recovery patterns of propulsion.

• Ocean and Polar Research
• /
• v.25 no.4
• /
• pp.625-631
• /
• 2003

While phytoplankton diversity and productivity in the Southern Ocean has been widely studied in recent years, most attention has been given to elucidating environmental factors that affect the dynamics of micro-plankton (mainly diatoms) and nano-plankton (mainly Phaeocystis antarctica). Only limited effects have been given to studying the occurrence and the potential risks associated with the blooming of dinoflagellates in the relevant waters. This study focused on the appearance and toxicological characteristics of a toxic dinoflagellate, Alexandrium tamarense, identified and isolated from the Drake Passage in a research cruise from November to December 2001 The appearance of A. tamarense in the Southern Ocean indicates the risk of a paralytic shellfish poisoning (PSP) outbreak there and is therefore of scientific concern. Results showed that while the overall quantity of A. tamarense in water samples from 30meters below the sea surface often comprised less than 0.1% of the total population of phytoplankton, the highest concentration of A. tamarense (20 cells $L^{-1}$) was recorded in the portion of the Southern Ocean between the southern end of South America and the Falkland Islands. Waters near the Polar Front contained the second highest concentrations of 10-15 cells $L^{-1}$. A. tamarense was however rarely found in waters near the southern side of the Polar Front, indicating that cold sea temperatures near the Antarctic ice does not favor the growth of this dinoflagellate. One strain of A. tamarense from this cruise was isolated and cultured for further study in the laboratory. Experiments showed that this strain of A. tamarense has a high tolerance to temperature variations and could survive at temperatures ranging from $5-26^{\circ}C$. This shows the cosmopolitan nature off. tamarense. With regard to the algal toxins produced, this strain of A. tamarense produced mainly C-2 toxins but very little saxitoxin and gonyailtoxin. The toxicological property of this A. tamarense strain coincided with a massive death of penguins in the Falkland Islands in December 2002 to January 2003.

• Imaging Science in Dentistry
• /
• v.42 no.3
• /
• pp.163-167
• /
• 2012

Purpose: ANSI/ADA has established standards for adequate radiopacity. This study was aimed to assess the changes in radiopacity of composite resins according to various tube-target distances and exposure times. Materials and Methods: Five 1-mm thick samples of Filtek P60 and Clearfil composite resins were prepared and exposed with six tube-target distance/exposure time setups (i.e., 40 cm, 0.2 seconds; 30 cm, 0.2 seconds; 30 cm, 0.16 seconds, 30 cm, 0.12 seconds; 15 cm, 0.2 seconds; 15 cm, 0.12 seconds) performing at 70 kVp and 7 mA along with a 12-step aluminum stepwedge (1 mm incremental steps) using a PSP digital sensor. Thereafter, the radiopacities measured with Digora for Windows software 2.5 were converted to absorbencies (i.e., A=-log (1-G/255)), where A is the absorbency and G is the measured gray scale). Furthermore, the linear regression model of aluminum thickness and absorbency was developed and used to convert the radiopacity of dental materials to the equivalent aluminum thickness. In addition, all calculations were compared with those obtained from a modified 3-step stepwedge (i.e., using data for the 2nd, 5th, and 8th steps). Results: The radiopacities of the composite resins differed significantly with various setups (p<0.001) and between the materials (p<0.001). The best predicted model was obtained for the 30 cm 0.2 seconds setup ($R^2$=0.999). Data from the reduced modified stepwedge was remarkable and comparable with the 12-step stepwedge. Conclusion: Within the limits of the present study, our findings support that various setups might influence the radiopacity of dental materials on digital radiographs.

• Ocean Science Journal
• /
• v.42 no.1
• /
• pp.31-39
• /
• 2007

Distributional characteristics of dinoflagellate cysts in surface sediments were investigated in relation to environmental factors in the eastern part of Geoje Island, Korea. Samples were collected from 10 stations in February of 2004 and water temperature and salinity were measured in February, May, September and November of 2004. Total 30 taxa of dinoflagellate cysts were identified representing 19 genera, 28 species and 2 unidentified species. Among these dinoflagellate cysts, Brigantedinium spp. of which relative proportion in th e total dinoflagellate cysts was 23.5%, was the most abundant at all stations except St. 1, and was followed by Spiniferites bulloideus (8.6%), Lingulodinium machaerophorum (8.2%) and Diplopsalis lenticula (6.7%). In addition, ellipsoidal cysts of the genus Alexandrium (Alexandrium catenella - tamarense type) and Gymnodinium catenatum, known to be causative organisms for PSP, occurred with high concentrations. Scrippsiella trochoidea was also found; however, its cyst concentration was low. Generally, species composition in the study area was similar to these reported from Jinhae Bay and Busan Harbor and several dinoflagellate cysts reflected the eutrophic condition. Cyst distribution in th e eastern part of Geoje Island seems to be influenced by the Tsushima Warm Current flowing from the southwest. The mean water temperature was $12.0^{\circ}C$ in February, $14.7^{\circ}C$ in May, $20.9^{\circ}C$ in September and $17.2^{\circ}C$ in November, which was most favorable for Alexandrium spp. growth. The abundances of dinoflagellate cysts rang e d from 528 to 2,834 cysts/g dry sediment. Higher concentrations were recognized in sediments of west area of the Jisimdo than at other stations. The cyst composition of this area was closely related to these of Jinhae Bay and Busan Harbor from which currents flow into this area. Higher cyst concentration in the west area of Jisimdo might be due to formation of the gyre.

• Journal of the Korean Institute of Gas
• /
• v.24 no.5
• /
• pp.1-9
• /
• 2020

In Korea, hydrogen stations are being promoted and commercialized. However, the risk assessment for the hydrogen station is not clear. In particular, it is not clear how to calculate the risk and acceptable criteria for a hydrogen station. Therefore, in this study, three hydrogen stations being installed were selected and general risks were calculated and the social risk of each hydrogen station was calculated. In general, the method of risk assessment is individual/social risk. This is an individual's death rate considering the frequency of accidents, And the likelihood of death according to the number of nearby residents. These can be used to calculate the level of risk for a hydrogen station. However, this method of calculate risks is the criteria for judging whether it is acceptable are unclear. For this reason, this study investigated the allowable standards for foreign risks and considered that they were acceptable by applying the risks of selected domestic hydrogen stations.

• KSBB Journal
• /
• v.16 no.6
• /
• pp.579-591
• /
• 2001

The cell growth inhibitor effect of cervical cancer cells was investigated by liposome mediated transfection (pRcCMVp53/lipofectin) and by transfection using adenovirus (AdCMVp57). The papilloma virus cancer cell lines we used in this study were HPV16 positive, having inhibiter gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3. LacZ gene of E.coli was used as the marker gene for the transfection efficiency. The effect on the inhibition of tumor cell growth was measured by cell count and cell viability though ELISA analysis and MTT assay. The inhibition of tumor cell growth was confirmed by measuring each assay for six days, comparing with the normal control cell growth. The cell growth of cervical cancer calls by transfection was significantly reduced and showed tittle differences among the cell lines. To eliminate the potential problem of Ad(adenovirus) contamination during rAAV production, rAAV can be produced by a triple transfection of vector plasmic, packaging plasmid, and adenovirus helper plasmid. To examine the helper functions of Ad plasmids on the production of rAAV vector, we carried out cotransfection of three plasmids, AAV vector, packaging construct, and Ad helper plasmids. The optimized transfection condition for calcium phosphate method is 25ug of total DNA per 10-cm-diameter plate of 293 cell. We found that rAAV yields peaked at 48hr after Ad infection. The titer of rAAV was measured by the dot blot analysis to measure the number of particles/ml based on the quantification of viral DNA. Recent1y, Kombucha(fungi) was identified as a very potent antileukefic agent. In the present study, effect of natural toxin(plankton) and Kombucha is PSP(GTXI-3, neoSTX), on various MTT assay cervical cancer cell line. Toxin(GTX 1-3, neoSTX) also inhibited the proliferation in primary cervical cancer calls in a dose-dependent toxin concentration. These results showed that toxin was very potent in inhibiting the proliferation of cervical cancer calls in vitro. Toxins and Kombuoha exhibited a dose dependent inhibition of cellular proliferation in cancer cell line.

• Journal of the Korean Society of Propulsion Engineers
• /
• v.12 no.5
• /
• pp.67-72
• /
• 2008

The film cooling effectiveness on a flat plate measured by pressure sensitive paint technique. Six film cooling hole were fabricated on a flat plate with 30 degree angle with respect to the surface and three blowing ratios of 0.5, 1, and 2 were tested. Results showed that PSP technique successfully evaluated the distribution of film cooling effectiveness and showed similar results with references. The film cooling effectiveness near the film cooling holes was higher for lower blowing ratio case. As the blowing ratio was increased, the film cooling effectiveness near the film cooling hole decreased due to the lift off of the coolant. At far downstream, the film cooling effectiveness for higher blowing ratio was higher due to the coolant reattachment.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2013.02a
• /
• pp.105-106
• /
• 2013

Surface plasmon resonance (SPR) is classified into the propagating surface plasmon (PSP) excited on flat metal surfaces and the local surface plasmon (LSP) excited by metalnanoparticles. It is known that fluorescence signals are enhanced by these two SPR-fields.On the other hand, fluorescence is quenched by the energy transfer to metal (FRET). Bothphenomena are controlled by the distance between dyes and metals, and the degree offluorescence enhancement is determined by the correlation. In this study, we determined thecondition to achieve the maximum fluorescence enhancement by adjusting the distance of ametal nanoparticle 2D sheet and a quantum dots 2D sheet by the use of $SiO_2$ spacer layers. The 2D sheets consisting of myristate-capped Ag nanoparticles (AgMy nanosheets) wereprepared at the air-water interface and transferred onto hydrophobized gold thin films basedon the Langmuir-Schaefer (LS) method [1]. The $SiO_2$ sputtered films with different thickness (0~100 nm) were deposited on the AgMy nanosheet as an insulator. TOPO-cappedCdSe/CdZnS/ZnS quantum dots (QDs, ${\lambda}Ex=638nm$) [2] were also transferred onto the $SiO_2$ films by the LS method. The layered structure is schematically shown in Fig. 1. The result of fluorescence measurement is shown in Fig. 2. Without the $SiO_2$ layer, the fluorescence intensity of the layered QD film was lower than that of the original QDs layer, i.e., the quenching by FRET was predominant. When the $SiO_2$ thickness was increased, the fluorescence intensity of the layered QD film was higher than that of the original QDs layer, i.e., the SPR enhancement was predominant. The fluorescence intensity was maximal at the $SiO_2$ thickness of 20 nm, particularly when the LSPR absorption wavelength (${\lambda}=480nm$) was utilized for the excitation. This plasmonic nanosheet can be integrated intogreen or bio-devices as the creation point ofenhanced LSPR field.

• Journal of the Korean Institute of Gas
• /
• v.28 no.1
• /
• pp.27-34
• /
• 2024

In accordance with the clean hydrogen-centered hydrogen industry development plan, technology development and demonstration operation of water electrolysis devices are being actively carried out. Risk assessments are performed on newly developed hydrogen-using facilities and supplies to identify potential hazard factors. However, in general, most industrial accidents are caused by operator's human error or incorrect work practices. Additionally, the possibility of accidents occurring during operation control, maintenance, and inspection increases. For this reason, for equipment developed for demonstration operation, the correct work methods for the main jobs of operator must be reviewed and work procedures must be prepared. Accordingly, in this study, we conducted a job safety analysis (JSA) on major jobs in the operation of water electrolysis facilities to study safe work methods and necessary measures.