• 한국건설순환자원학회논문집
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• 제9권4호
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• pp.594-601
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• 2021

본 연구에서는 철강산업의 부산물인 제강슬래그의 도로포장용 건설재료로 사용 확대를 위한 골재의 품질 특성을 연구하는 것으로 골재의 녹물 발생 가능성을 확인하고, 아스팔트 콘크리트 혼합물의 내구성능과 흡음률 평가를 통한 소음 저감 특성을 평가하였다. 골재의 녹물 발생 실험을 수행한 결과, 두 골재에서 녹물이 관찰되지 않아 실제 도로의 수환경에서 녹물 발생 가능성이 매우 낮을 것으로 판단된다. 수분저항성 실험을 수행한 결과, 모든 혼합물에서 85%를 상회하는 인장강도 비를 보였으며 아스팔트 혼합물 기준을 만족하였다. 또한, 제강슬래그를 활용한 아스팔트 포장도로의 구조적 안정성이 일반 포장도로 대비 우수한 것으로 조사되었다. 제강슬래그 혼합물의 흡음 계수는 일반 골재 혼합물보다 높게 측정되었다. 이에, 도로 소음 저감에 제강슬래그 혼합물이 일반 혼합물보다 효과적으로 대응할 수 있을 것으로 판단된다.

• 수산경영론집
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• 제53권3호
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• pp.43-64
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• 2022

Efforts to prevent IUU fishing began in 1996 as the IUU Antarctic Marine Living Resources Conservation Committee reported the multilateral efforts of the international community and related international organizations to prevent IUU fishing. Korea has recently been pointed out by the international community as to many problems regarding its will to eradicate IUU fishing. It is true that Korea has ever been designated as an 'IUU participating country' or a 'non-cooperative third country' by the international community and that there have been considerable difficulties in exporting seafood and using ports along with the deterioration of the national image. In 2020, with the efforts of related organizations and fisheries companies, Korea is now free from being known as a that Korea has recovered some degrees of trust from the international community through strengthening legal sanctions against IUU fishing and thorough implementation of follow-up measures is now free from non-cooperating country it cannot be said that the basic problems have been completely resolved just because it has emerged as a disgraceful country, and the current state of IUU fishing of Korea leaves a room for designation as a 'non-cooperative third country' again at any time in the future. Accordingly, there is an urgent need to examine the problems of the IUU fishing-related legal system in Korea and to come up with an improvement plan. Therefore, this paper reviews international norms for IUU fishing regulation (PSMA etc.) and domestic laws with the Distant Water Fisheries Development Act and Propose the improvement methods for related legal systems in Korea.

• Journal of Chest Surgery
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• 제37권12호
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• pp.967-977
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• 2004

배경: DNA 메칠화란 유전자의 Promoter에 있는 CpG dinucleotide의 cytosine기에 메칠기가 붙는 현상을 말한다. CpG dinucleotide에 과메틸화가 일어나면 일부 유전자의 발현이 감소되며, 그 반대로 CpG dinucleotide의 메칠화가 억제되면 유전자 발현이 증가된다. DNA 메칠화 억제제인 5-aza-2'- deoxycytidine (ADC)을 폐암세포에 처치했을 때 암항원 유전자의 발현 유무와 이를 위한 최적 조건을 조사하고, 아울러 MHC와 B7의 발현과 세포 성장에 미치는 영향을 조사하여 암치료 백신에 ADC를 임상적으로 이용할 수 있는 지를 연구하였다. 대상 및 방법: 4개의 사람 폐암세포주 (NCIH1703, NCIH522, MRC-5 및 A549)에 ADC를 1 uM 농도로 처치한 후 48시간 뒤에 MAGE family, GAGE, NY-ESO-1, PSMA, CEA 및 SCC항원 유전자에 대한 RT-PCR을 실시하였고, 폐암세포에서 암항원의 발현을 증가시키는 최적의 ADC처치 조건을 규명하기 위하여 ADC농도와 처치 시간을 다양하게 하여 암세포를 자극한 후 암항원 유전자 발현성을 분석하였다. 또한 ADC 처리가 폐암 세포주의 MHC와 B7 발현을 증가시키는 가를 알아보기 위해 1 uM 농도의 ADC를 72시간 처치한 후 FACS 분석을 실시하였고, ADC가 세포성장에 미치는 영향을 알아보기 위하여, ADC를 0.2, 1 및 5 uM 농도로 96시간 처치 후 세포수를 측정하여 상대성장지수를 조사하였다. 결과: 세포주에 따라 차이는 있으나 MAGE, GAGE, NY-ESO-1 및 PSMA의 발현이 유도되었으며, MAGE아형 중에는 MAGE-1, -2, -3, -4, -6으로 나타났다. 그러나 비암항원인 CEA발현은 변화가 없었으며 SCC항원 유전자의 발현은 오히려 ADC처치에 의해 감소되었다. ADC 처치 후 24∼48 시간이 지난 뒤부터 암항원 유전자의 발현이 증가하였으며 ADC처리에 의해 유도된 유전자의 발현성은 ABC처치 후 최소 14일까지 유지되었다. 또 ADC를 0.2, 1, 5 uN 농도로 첨가하여 48시간 배양한 후 암항원 유전자 발현성을 측정한 결과 세포주에 따라 다소 차이는 있으나 대개 0.2 uM농도에서도 유전자 발현이 유도되었으며 1, 5 uM농도에서 매우 강하게 유도되었다. ADC 처리가 페암세포주의 MHC와 B7 발현을 증가시키는가를 알아보기 위해 1 uM 농도의 ADC를 72시간 처치한 후 FACS 분석을 실시한 결과 4개의 페암세포주에서 MHC 및 B7분자의 발현은 유도되지 않았다. 또 ADC농도가 세포성장에 미치는 영향을 알아보기 위하여 ADC를 0.2, 1, 5 uM농도로 96시간 처치 후 세포수를 측정하여 상대성장지수를 알아본 결과 ADC 처치 농도가 증가함에 따라 세포의 성장은 매우 감소하였다. 결론: 폐암세포주에서 ADC처치는 MAGE, GAGE 및 NY-ESO-1과 같은 세포독성 T 림프구 반응을 유도할 수 있는 암항원의 발현을 증가시킬 수 있으며, ADC의 세포독성과 항원 발현 유발시간을 분석할 때 1 uM 농도에서 48시간 처치한 후 ADC가 없는 배지에서 수일간 배양하는 것이 가장 효과적이라고 생각된다. 그러나, ADC를 처치하여도 MHC 및 B7의 발현의 변화는 없었으므로 ADC를 처치한 폐암세포를 암백신으로 사용하기 위해서는 MHC나 B7 및 cytokine의 발현을 증가시키는 추가적인 처치가 필요하다고 생각된다.

• 대한토목학회논문집
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• 제37권6호
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• pp.1043-1051
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• 2017

노후 된 도로 포장 개량을 위한 아스팔트 덧씌우기 포장의 경우 반사균열로 인한 조기파손이 문제가 되고 있어, 반사균열을 억제하고 포장의 내구성을 확보할 수 있는 새로운 공법이 필요한 실정이다. 에폭시 아스팔트 바인더를 이용한 응력흡수층(SAMI)을 통해 아스팔트 덧씌우기 포장의 내구성을 확보하고자 하였다. 에폭시 응력흡수층의 인장성능, 접착성능, 방수성능, 내화학성, 내구성 실험을 수행하였으며, 교면 방수재료의 품질기준을 만족하는 것으로 나타났다. 반사균열 억제 효과를 검토하기 위해 반복직접인장실험 결과 에폭시 응력흡수층 적용시 단면 두께가 감소하여도 두께 10cm의 PSMA 아스팔트 콘크리트에 비해 1.2~1.56배 높은 반사균열저항성을 나타내었다. 4점 빔피로 실험 결과 에폭시 응력흡수층을 적용하면 피로파괴횟수가 약 7.5배 높아져 포장의 내구성을 향상시킬 수 있었다. 에폭시 응력흡수층은 반사균열 억제와 피로수명 향상 및 방수층으로서의 역할을 수행하여 아스팔트 덧씌우기 포장의 내구성 향상에 효과가 있는 것으로 나타났다.

• 대한방사성의약품학회지
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• 제9권1호
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• pp.17-21
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• 2023

In this study, we evaluated the targeting of prostate cancer (PCa) using [¹⁸F]Florastamin in non-clinical study, for the purpose of therapeutic monitoring of [¹⁷⁷Lu]Ludotadipep, a therapeutic radiopharmaceutical for PCa, [¹⁸F]Florastamin/[¹⁷⁷Lu]Ludotadipep was co-administered to a single-individual prostate tumor bearing mouse model, mimicking clinical condition. Considering the difference in half-life of the two isotopes (¹⁸F or ¹⁷⁷Lu), image scan of whole-body autoradiography was performed at 24 or 48 h after preparation of frozen section, respectively. Then, it was confirmed whether they showed the same targeting efficiency for the area of tumor. A tumor xenograft model was prepared using PSMA-overexpressing PC3-PIP prostate cancer cells. [¹⁸F]Florastamin [111 MBq (3 mCi) in 100 µL]/¹⁷⁷Lu]Ludotadipep [3.7 MBq (100 µCi) in 100 µL] was co-administered through the tail vein, and 2 hours after administration, the mice were frozen, and after freezing for 24 hours, whole-body cryosection was performed at 24 h after freezing. Image scanning using cryosection was performed after 24 or 48 hours after freezing, respectively. In the scan image after 24 hours, tumor uptake of [¹⁸F] Florastamin/[¹⁷⁷Lu]Ludotadipep were simultaneously observed specific uptake in the tumor. In the scan image after 48 hours in the same section, signal of ¹⁸F was lost by decay of radioisotope, and specific uptake image for [¹⁷⁷Lu]Ludotadipep was observed in the tumor. Uptake of [¹⁷⁷Lu]Ludotadipep was specific to the same tumor region where [¹⁸F]Florastamin/[¹⁷⁷Lu]Ludotadipep was uptake. These results suggested that [¹⁸F]Florastamin showed the same tumor uptake efficiency to PCa as [¹⁷⁷Lu]Ludotadipep, and effective therapeutic monitoring is expected to be enable using [¹⁸F]Florastamin during [¹⁷⁷Lu]Ludotadipep therapy for PCa.

• Korean Journal of Radiology
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• 제25권2호
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• pp.179-188
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• 2024

Objective: ¹⁷⁷Lutetium [Lu] Ludotadipep is a novel prostate-specific membrane antigen targeting therapeutic agent with an albumin motif added to increase uptake in the tumors. We assessed the biodistribution and dosimetry of [¹⁷⁷Lu]Ludotadipep in patients with metastatic castration-resistant prostate cancer (mCRPC). Materials and Methods: Data from 25 patients (median age, 73 years; range, 60-90) with mCRPC from a phase I study with activity escalation design of single administration of [¹⁷⁷Lu]Ludotadipep (1.85, 2.78, 3.70, 4.63, and 5.55 GBq) were assessed. Activity in the salivary glands, lungs, liver, kidneys, and spleen was estimated from whole-body scan and abdominal SPECT/CT images acquired at 2, 24, 48, 72, and 168 h after administration of [¹⁷⁷Lu]Ludotadipep. Red marrow activity was calculated from blood samples obtained at 3, 10, 30, 60, and 180 min, and at 24, 48, and 72 h after administration. Organand tumor-based absorbed dose calculations were performed using IDAC-Dose 2.1. Results: Absorbed dose coefficient (mean ± standard deviation) of normal organs was 1.17 ± 0.81 Gy/GBq for salivary glands, 0.05 ± 0.02 Gy/GBq for lungs, 0.14 ± 0.06 Gy/GBq for liver, 0.77 ± 0.28 Gy/GBq for kidneys, 0.12 ± 0.06 Gy/GBq for spleen, and 0.07 ± 0.02 Gy/GBq for red marrow. The absorbed dose coefficient of the tumors was 10.43 ± 7.77 Gy/GBq. Conclusion: [¹⁷⁷Lu]Ludotadipep is expected to be safe at the dose of 3.7 GBq times 6 cycles planned for a phase II clinical trial with kidneys and bone marrow being the critical organs, and shows a high tumor absorbed dose.

• Korean Journal of Radiology
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• 제23권9호
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• pp.911-920
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• 2022

Objective: ⁶⁸Ga-NGUL is a novel prostate-specific membrane antigen (PSMA)-targeting tracer based on Glu-Urea-Lys derivatives conjugated to a 1,4,7-triazacyclononane-N,N',N''-triacetic acid (NOTA) chelator via a thiourea-type short linker. This phase I clinical trial of ⁶⁸Ga-NGUL was conducted to evaluate the safety and radiation dosimetry of ⁶⁸Ga-NGUL in healthy volunteers and the lesion detection rate of ⁶⁸Ga-NGUL in patients with prostate cancer. Materials and Methods: We designed a prospective, open-label, single-arm clinical trial with two cohorts comprising six healthy adult men and six patients with metastatic prostate cancer. Safety and blood test-based toxicities were monitored throughout the study. PET/CT scans were acquired at multiple time points after administering ⁶⁸Ga-NGUL (2 MBq/kg; 96-165 MBq). In healthy adults, absorbed organ doses and effective doses were calculated using the OLINDA/EXM software. In patients with prostate cancer, the rates of detecting suspicious lesions by ⁶⁸Ga-NGUL PET/CT and conventional imaging (CT and bone scintigraphy) during the screening period, within one month after recruitment, were compared. Results: All 12 participants (six healthy adults aged 31-32 years and six prostate cancer patients aged 57-81 years) completed the clinical trial. No drug-related adverse events were observed. In the healthy adult group, ⁶⁸Ga-NGUL was rapidly distributed, with the highest uptake in the kidneys. The median effective dose coefficient was calculated as 0.025 mSv/MBq, and cumulative activity in the bladder had the highest contribution. In patients with metastatic prostate cancer, 229 suspicious lesions were detected using either ⁶⁸Ga-NGUL PET/CT or conventional imaging. Among them, ⁶⁸Ga-NGUL PET/CT detected 199 (86.9%) lesions and CT or bone scintigraphy detected 114 (49.8%) lesions. Conclusion: ⁶⁸Ga-NGUL can be safely applied clinically and has shown a higher detection rate for the localization of metastatic lesions in prostate cancer than conventional imaging. Therefore, ⁶⁸Ga-NGUL is a valuable option for prostate cancer imaging.

• BMB Reports
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• 제44권9호
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• pp.578-583
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• 2011

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are biochemical markers used to test for liver diseases. Copy number variation (CNV) plays an important role in determining complex traits and is an emerging area in the study various diseases. We performed a genome-wide association study with liver function biomarkers AST and ALT in 407 unrelated Koreans. We assayed the genome-wide variations on an Affymetrix Genome-Wide 6.0 array, and CNVs were analyzed using HelixTree. Using single linear regression, 32 and 42 CNVs showed significance for AST and ALT, respectively (P value < 0.05). We compared CNV-based genes between the current study (KARE2; AST-140, ALT-172) and KARE1 (AST-1885, ALT-773) using NetBox. Results showed 9 genes (CIDEB, DFFA, PSMA3, PSMC5, PSMC6, PSMD12, PSMF1, SDC4, and SIAH1) were overlapped for AST, but no overlapped genes were found for ALT. Functional gene annotation analysis shown the proteasome pathway, Wnt signaling pathway, programmed cell death, and protein binding.

• 한국도로학회논문집
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• 제18권1호
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• pp.91-97
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• 2016

PURPOSES : The objective of this paper is to develop a pavement performance model based on the Bayesian algorithm, and compare the measured and predicted performance data. METHODS : In this paper, several pavement types such as SMA (stone mastic asphalt), PSMA (polymer-modified stone mastic asphalt), PMA (polymer-modified asphalt), SBS (styrene-butadiene-styrene) modified asphalt, and DGA (dense-graded asphalt) are modeled in terms of the performance evaluation of pavement structures, using the Bayesian algorithm. RESULTS : From case studies related to the performance model development, the statistical parameters of the mean value and standard deviation can be obtained through the Bayesian algorithm, using the initial performance data of two different pavement cases. Furthermore, an accurate performance model can be developed, based on the comparison between the measured and predicted performance data. CONCLUSIONS : Based on the results of the case studies, it is concluded that the determined coefficients of the nonlinear performance models can be used to accurately predict the long-term performance behaviors of DGA and modified asphalt concrete pavements. In addition, the developed models were evaluated through comparison studies between the initial measurement and prediction data, as well as between the final measurement and prediction data. In the model development, the initial measured data were used.

• 한국보건간호학회지
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• 제34권3호
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• pp.473-489
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• 2020

Purpose: This study investigated the patient safety culture (PSC), the perception of importance on patient safety management (PIPSM) and the patient safety management activities (PSMA) of care workers in nursing homes. This was a descriptive study that attempted to provide basic data for the patient safety education program of care workers. Methods: Data were collected using questionnaires and interviews from July 1 to 31 in 2020. One hundred and seventy-four care workers participated in quantitative research. The collected data were analyzed by the SPSS/WIN 25.0 program using descriptive statistics, t-test, ANOVA, Bonferroni, and Pearson's correlation. The qualitative data were collected through semi-structured, audio-recorded interviews with six representatives and six care workers from six nursing homes. Content analysis was performed to analyze the data. Results: Positive correlations were observed between PSC and PIPSM, and between PIPSM and PIPSM. Care workers' experience in patient safety management was in the following six categories: "Safety accident risk factors", "Type of safety accidents", "How to prevent safety accidents", "Effective safety management education", "Emphasis on occupational ethics of care workers", and "Needs for standard protocol" Conclusion: These findings indicate that considering the care workers' age and facility size, nurses should enhance patient safety education for care workers and establish a management activity system.