• Title/Summary/Keyword: PSB

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Influencing Factors on Patient Safety Behaviors of Care Workers in Long Term Care Facilities (요양보호사의 환자안전행위에 관한 영향요인)

  • Moon, Myeong Ja;Lee, Seonhye
    • Journal of Korean Public Health Nursing
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    • v.33 no.1
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    • pp.98-108
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    • 2019
  • Purpose: The purpose of this study was to identify factors influencing care workers' patient safety behaviors (PSB), we hoped to provide baseline data for its activation. Methods: The subjects of this study were chosen by convenience sampling, which included 114 care workers at long term care facilities. Data were collected from August 22 to October 10, 2018. The gathered data were analyzed using the SPSS program, which was used to Cronbach's alpha, descriptive statistics, t-test, ANOVA, Pearson correlation coefficient and stepwise regression. Results: The care workers' average PSB was at 7.81 points, patient safety culture (PSC) 3.86 points and safety of long-term care facilities 7.07. PSB positively correlated with safety of facilities(r=.802, p<.001) and PSC(r=.558, p<.001). Work attitude and satisfaction of job were explained 35.1% of the PSB. Conclusion: Administrators of long term care facilities should be interested in patient safety culture and improving job satisfaction of care workers in order to improve patient safety behaviors.

A report of the second chloroplast genome sequence in Veronica nakaiana (Plantaginaceae), an endemic species in Korea

  • LEE, Yae-Eun;LEE, Yoonkyung;KIM, Sangtae
    • Korean Journal of Plant Taxonomy
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    • v.51 no.1
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    • pp.109-114
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    • 2021
  • Veronica nakaiana Ohwi (Plantaginaceae) is an endemic taxon on Ulleungdo Island, Korea. We report the second complete chloroplast genome sequence of V. nakaiana. Its genome size is 152,319 bp in length, comprising a large single-copy of 83,195 bp, a small single-copy of 17,702 bp, and a pair of inverted repeat regions of 25,711 bp. The complete genome contains 115 genes, including 51 protein-coding genes, four rRNA genes, and 31 tRNA genes. When comparing the two chloroplast genomes of V. nakaiana, 11 variable sites are recognized: seven SNPs and four indels. Two substitutions in the coding regions are recognized: rpoC2 (synonymous substitution) and rpl22 (nonsynonymous substitution). In nine noncoding regions, one is in the tRNA gene (trnK-UUU), one is in the intron of atpF, and seven are in the intergenic spacers (trnH-GUG~psbA, trnK-UUU, rps16~trnQ-UUG, trnC-GCA~petN, psbZ~trnG-GCC, ycf3~trnS-GGA, ycf4~cemA, and psbB~psbT). The data provide the level of genetic variation in V. nakaiana. This result will be a useful resource to formulate conservation strategies for V. nakaiana, which is a rare endemic species in Korea.

Effects of Popped Soybean on Concentration of Ruminal Peptide and Blood Amino Acids in Holstein Calves

  • Kim, H.D.;Ha, J.K.;Itabashi, H.;Kim, S.W.;Kim, W.Y.;Ko, Y.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.2
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    • pp.155-161
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    • 1998
  • This study conducted to evaluate effects of popped soybean on levels of ruminal peptides and blood amino acids in Holstein calves fed sudan grass hay as a forage source and popped (PSB) soybean as a concentrate supplement. At 0, 2, 4 and 6 h after feeding, rumen fluid and blood samples were collected from the rumen and jugular vein, respectively, and amino acids, peptides and other nitrogen-containing compounds in the rumen were analyzed. Ruminal pH tended to be higher in the RSB than in the PSB treatments, and declined upto 4 h after feeding, since then increased in both treatments. The concentrations of ammonia-N in all treatments increased upto 2 h after feeding, and then decreased gradually with time after feeding. The concentrations of ammonia N in the rumen were not significantly different between the treatments, however, those in RSB treatment appeared to be higher. Also, protein concentrations in the rumen were not significantly different between the treatments. Peptide productions were the highest at 2 h after feeding in the group fed RSB which is rapidly degradable in rumen, whereas those in the group fed PSB which is slowly degradable in rumen were maximized at 4 h after feeding. The concentration of total free essential amino acids in plasma was higher in the RSB treatment than in the PSB, but disappearance rates of these amino acids out of plasma was higher in the PSB treatment than in the RSB treatment. Disappearance rates of free non-essential amino acids in plasma were not significantly different between the treatments. Consequently, this study implies that the production of peptide and utilization of blood amino acid may be controlled by the modification of protein degradability.

Effect of Co-inoculation of Two Bacteria on Phosphate Solubilization

  • Lee, Yu-Jin;Lee, Heon-Hwak;Lee, Chan-Jung;Yoon, Min-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.4
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    • pp.318-326
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    • 2016
  • Two phosphate solubilizing bacteria, Pantoea rodasii PSB-11and Enterobacter aerogenes PSB-12, were isolated from button mushroom compost and employed to assess their synergistic effect in liquid medium and on growth of green gram plants by single and co-inoculation of the strains. Co-inoculation of two strains was found to release the highest content of soluble phosphorus ($521{\mu}g\;ml^{-1}$) into the medium, followed by single inoculation of Pantoea strain ($485{\mu}g\;ml^{-1}$) and Enterobacter strain ($470{\mu}g\;ml^{-1}$). However, there was no significant difference between single inoculation of bacterial strain and co-inoculation of two bacterial strains in terms of phosphorous release. The highest pH reduction, organic acid production and glucose consumption was observed in the E. aerogenes PSB-12 single inoculated culture medium rather than those of co-inoculation. According to the plant growth promotion bioassay, co-inoculated mung bean seedlings recorded 10.6% and 10.7% higher shoot and root growth respectively compared to the control. Therefore, in concluding, co-inoculation of the strains P. rodasii and E. aerogenes displayed better performance in stimulating plant growth than inoculation of each strain alone. However, being short assessment period of the present study, we recommend in engaging further works under field conditions in order to test the suitability of the strains to be used as bio-inoculants.

Glyphosate Toxicity: III. Detection of QB Protein in Thylakoid Membrane of Tomato Apical Meristem Using an Antibody Raised from Hybrid Protein of psb A and lac Z Gene (Glyphosate 독성: III. psb A와 lac Z 유전자의 Hybrid 단백질로부터 만들어진 항체를 이용한 토마토 정단분열조직의 Thylakoid막 내 QB 단백질의 검정)

  • Kim, Tae-Wan;Amrhein, Nikolaus
    • Korean Journal of Weed Science
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    • v.15 no.3
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    • pp.206-213
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    • 1995
  • Glyphosate(N-[phosphonomethyl]glycine) applied to the assimilate-exporting leaves(i.e. third old leaf) of tomato(Lycopersicon esculentum Mil var. Moneymaker). Herbicide binding protein, QB protein(D1), has been immunoblotted using the antibodies raised against the hybrid-protein expressed by a part of spinach psb A gene cloned in frame with the 3'end of lac Z gene to allow expression of the ${\beta}$-galactosidase(EC 3.21.23) in Escherichia coli. Glyphosate has an effect on a turnover of D1 within photosystem II of thylakoid membrane. The dysfunction of D1 protein within light harvesting complex(LHC-II) seems to be a pleiotropic effect of glyphosate.

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Analysis of Wave Transformation and Velocity Fields Including Wave Breaking due to the Permeable Submerged Breakwaters (수중투과성구조물에 의한 쇄파를 수반한 파랑변형 및 유속장 해석)

  • 김도삼;이광호;김정수
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.14 no.2
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    • pp.171-181
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    • 2002
  • Among various numerical methods of wave transformations including wave breaking by structure, models using VOF(Volume Of Fluid) method to trace free surface are getting into the spotlight recently. In order to analyze wave transformations and velocity of the wave fields due to the permeable submerged breakwater(PSB), This study applied VOF method to the two-dimensional wave channel installed line-source to generate waves and added dissipation zone to offer a non-reflective boundary. Hydraulic experiments was performed to obtain the application of two-dimensional numerical wave channel. The results of numerical experiments using the two-dimensional wave channel agree well with the experimental data. It was shown that vortices are formed behind the PSB, and in case of the 2-rowed PSB they also are occurred in between PSBs, strongly non-linear waves are developed on the crown of the PSB, and the direction of velocities in porous media is determined by the shape of free surface.

Phylogenetic study of the Genus Suaeda(Chenopodiaceae) based on chloroplast and nuclear DNA sequences from Korea (엽록체 DNA 및 핵 DNA 염기서열에 근거한 한국산 나문재속(명아주과)의 분류학적 연구)

  • Kim, Suk-Kyu;Chung, Sang Ok
    • Korean Journal of Environment and Ecology
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    • v.32 no.6
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    • pp.566-574
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    • 2018
  • The purpose of this study was to identify the phylogenetic relationships of the plants in the Korean genus Suaeda and to find out the molecular markers that could confirm the interspecies relationships in the family tree through molecular phylogenetic studies. We used the nuclear ribosomal DNA ITS and the chloroplast DNA matK, psbA-trnH, and trnL-trnF as the molecular markers. We could not distinguish between S. japonica and S. maritima and between S. maritima and S. australis in the ITS region and could not distinguish between S. japonica and S. australis with the base sequence in the psbA-trnH and trnL-trnF region. However, we analyzed the combinations of four molecular marker regions and confirmed that each of five plant species of the genus Suaeda formed the independent line. Therefore, it is considered that combinations of molecular markers would be useful for the analysis of phylogenetic relationships in the genus Suaeda. Further investigations of the ecological and morphological characteristics would be needed to understand the phylogenetic relationship and lineage diversification in the genus Suaeda.

Isolation and Identification of Phosphate Solubilizing Bacteria from Chinese Cabbage and Their Effect on Growth and Phosphorus Utilization of Plants

  • Poonguzhali, Selvaraj;Madhaiyan, Munusamy;Sa, Tong-Min
    • Journal of Microbiology and Biotechnology
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    • v.18 no.4
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    • pp.773-777
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    • 2008
  • Phosphate solubilizing bacteria (PSB) were isolated from the rhizosphere of Chinese cabbage and screened on the basis of their solubilization of inorganic tricalcium phosphate in liquid cultures. Ten strains that had higher solubilization potential were selected, and they also produced indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and siderophores. The strains were identified to be members of Pseudomonas, by 16S rDNA sequence analysis. Seed bacterization with PSB strains increased the root elongation and biomass of Chinese cabbage in seedling culture, although they had no effect on phosphorus uptake of plants. The plant growth promotion by PSB in this study could be due to the production of phytohormones or mechanisms other than phosphate solubilization, since they had no effect on P nutrition.

Morphology and Molecular Phylogeny of Psilothallia dentata (Ceramiaceae, Rhodophyta)

  • Yang, Eun-Chan;Kim, Kyung-Mi;Runess, Jan;Boo, Sung-Min
    • ALGAE
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    • v.19 no.4
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    • pp.283-292
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    • 2004
  • Psilothallia is a ceramiaceous red algal. genus that includes three species worldwide: P. dentate, P. siliculosa, and P. striate. The latter two species are limited to Australian waters, and P. dentate occurs in Japan. We here report the detailed morphology of P. dentate, and also determined plastid protein-coding psbA in P. dentate and putative relatives. Psilothallia dentate is distinguished by compressed thalli with alternate-distichous determinate branchlets, six periaxial cells, rhizoidal filaments in axes, cystocarps with 7-8 involucral filaments, spermatangia on branched filaments, and tetrahedrally divided tetrasporangia on branched filaments. Psilothallia dentate is also unusual in that cystocarps, spermatangial clusters, and tetrasporangial tufts are formed on short adventitious indeterminate branches arising on axils of determinate branchlets. The phylogenetic trees of psbA sequences show that P. dentata was nested in a monophyletic Glade comprising Ptilota, Neoptilota, and Plumaria. This result suggests that the taxonomic position of P. dentate may be transferred from the tribe Rhodocallideae to the Ptiloteae.

Efects of Chitosan on Cell Flocculation in Soybean Curd Wastewater Treated by Photosynthetic Bacteria (Chitosan에 의한 광합성세균 처리 두부공업폐수의 균체 응집효과)

  • 오준현;조홍연;양한철
    • Microbiology and Biotechnology Letters
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    • v.23 no.6
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    • pp.763-769
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    • 1995
  • As a mean to recover photosynthetic bacterial(PSB) cells and its practical uses in food industrial wastewater treatment, various biodegradable polyelectrolytes were first investigated for flocculation of suspended colloids in the PSB treatment process of soybean curd wastewater. Anionic polyelectrolytes such as sodium alginate and carrageenan were not effective but a cationic polyelectrolyte chitosan isolated from Portunus trituberclatus showed very effective flocculation activity. The concentration of chitosan, pH and temperature of wastewater for maximal flocculation were 40 mg/l, pH 7 and room temperature, respectively. Test using deacetylated chitosan to various degree showed higher flocculating activities in samples deacetylated over 75% and time for maximum flocculation was 40 min by stirring slowly under the above optimal conditions. Chitosan was not only effective to flocculate cells but also removed COD and MLSS of the wastewater. COD of 42% and MLSS of 87% were removed by addition of chitosan to the soybean curd wastewater treated with PSB.

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