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Effect of dietary carbohydrate sources on apparent nutrient digestibility of olive flounder (Paralichthys olivaceus) feed

  • Rahman, Md Mostafizur;Lee, Kyeong-Jun;Lee, Sang-Min
    • Fisheries and Aquatic Sciences
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    • v.19 no.4
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    • pp.15.1-15.5
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    • 2016
  • Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, nitrogen-free extract, and energy in selected carbohydrate sources including wheat flour (WF), ${\alpha}-potato$ starch (PS), ${\alpha}-corn$ starch (CS), Na alginate (AL), dextrin (DEX), and carboxymethyl cellulose (CMC) were determined for olive flounder. The olive flounder averaging $150{\pm}8.0g$ were held in 300-L tanks at a density of 30 fish per tank. Chromic oxide was used as the inert marker. Feces were collected from the flounder by a fecal collector attached to a fish rearing tank. Apparent dry matter and energy digestibilities of flounder fed WF, PS, CS, and DEX diets were significantly higher than those of fish fed AL and CMC diets. Apparent crude protein digestibility coefficients of flounder fed PS and CS diets were significantly higher than those of fish fed AL, DEX, and CMC diets. Apparent crude lipid and nitrogen-free extract digestibility coefficients of flounder fed PS and DEX diets were significantly higher than those of fish fed WF, CS, AL, and CMC diets. The present findings indicate that PS and DEX could be effectively used as dietary carbohydrate energy compared to WF, CS, AL, and CMC for olive flounder.

Crystal Structure and Biochemical Characterization of Xylose Isomerase from Piromyces sp. E2

  • Son, Hyeoncheol Francis;Lee, Sun-Mi;Kim, Kyung-Jin
    • Journal of Microbiology and Biotechnology
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    • v.28 no.4
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    • pp.571-578
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    • 2018
  • Biofuel production using lignocellulosic biomass is gaining attention because it can be substituted for fossil fuels without competing with edible resources. However, because Saccharomyces cerevisiae does not have a ${\text\tiny{D}}$-xylose metabolic pathway, oxidoreductase or isomerase pathways must be introduced to utilize ${\text\tiny{D}}$-xylose from lignocellulosic biomass in S. cerevisiae. To elucidate the biochemical properties of xylose isomerase (XI) from Piromyces sp. E2 (PsXI), we determine its crystal structure in complex with substrate mimic glycerol. An amino-acid sequence comparison with other reported XIs and relative activity measurements using five kinds of divalent metal ions confirmed that PsXI belongs to class II XIs. Moreover kinetic analysis of PsXI was also performed using $Mn^{2+}$, the preferred divalent metal ion for PsXI. In addition, the substrate-binding mode of PsXI could be predicted with the substrate mimic glycerol bound to the active site. These studies may provide structural information to enhance ${\text\tiny{D}}$-xylose utilization for biofuel production.

Control of Enzymatic Degradability of Biodegradable Polylactide by Blending with Non-degradable Polymers (비 분해성고분자와 블렌드를 이용한 생분해성 폴리유산의 효소분해속도 조절)

  • Jang, Seong-Ho;Park, Sang-Bo;Lee, Won-Ki
    • Journal of Environmental Science International
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    • v.19 no.9
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    • pp.1161-1167
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    • 2010
  • The effects of addition of non degradable polymers, polystyrene (PS) and poly(methyl methacrylate) (PMMA) on the rate of enzymatic degradation of biodegradable poly(l-lactide) (PLLA) have been studied in term of surface structure. Since a component in multicomponent polymeric system has shown surface enrichment, PS and PMMA which have lower surface energy than PLLA were selected as a minor blend component (5 wt%). Enzymatic degradation was carried out at $37^{\circ}C$ and pH 8.5 in the aqueous solution of Proteinase K. Two blend systems, partially miscible (PS/PLLA) and immiscible (PMMA/PLLA), showed the surface enrichment of 4 and 2 times of PS and PMMA, respectively. From the weight loss profile data, the slow degradation rate of both blend films was observed. This indicates that PS or PMMA domains which exist at surface act as a retardant of enzymatic attack.

Fabrication of ZnO Nanorod/polystyrene Nanosphere Hybrid Nanostructures by Hydrothermal Method for Energy Generation Applications (에너지 발생소자응용을 위한 수열합성법기반 ZnO 나노로드/Polystylene 하이브리드 나노구조 제조)

  • Baek, Seong-Ho;Park, Il-Kyu
    • Journal of Powder Materials
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    • v.22 no.6
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    • pp.391-395
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    • 2015
  • We report on the successful fabrication of ZnO nanorod (NR)/polystyrene (PS) nanosphere hybrid nanostructure by combining drop coating and hydrothermal methods. Especially, by adopting an atomic layer deposition method for seed layer formation, very uniform ZnO NR structure is grown on the complicated PS surfaces. By using zinc nitrate hexahydrate $[Zn(NO_3)_2{\cdot}6H_2O]$ and hexamine $[(CH_2)_6N_4]$ as sources for Zn and O in hydrothermal process, hexagonal shaped single crystal ZnO NRs are synthesized without dissolution of PS in hydrothermal solution. X-ray diffraction results show that the ZnO NRs are grown along c-axis with single crystalline structure and there is no trace of impurities or unintentionally formed intermetallic compounds. Photoluminescence spectrum measured at room temperature for the ZnO NRs on flat Si and PS show typical two emission bands, which are corresponding to the band-edge and deep level emissions in ZnO crystal. Based on these structural and optical investigations, we confirm that the ZnO NRs can be grown well even on the complicated PS surface morphology to form the chestnut-shaped hybrid nanostructures for the energy generation and storage applications.

PEDOT:PSS의 성막방법에 따른 유기태양전지용 ITO 투명전극과 PEDOT:PSS의 계면반응 연구

  • Kim, Hyo-Jung;Lee, Ju-Hyeon;Kim, Han-Gi
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.329-329
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    • 2013
  • 본 연구에서는 PEDOT:PSS와 crystalline-ITO (c-ITO) 박막 계면에서의 화학적 반응을(박리 및 용해 특성)을 관찰하기위해 spin-coating 및 droplet dropping을 통하여 PEDOT:PSS 용액을 코팅하고 이후 화학적 거동에 따른 전기적, 광학적 및 구조적 특성 변화를 관찰하였다. 강산성을 띄는 PEDOT:PSS (Al 4083) 박막의 코팅 전, 0.4T sodalime glass 위에 열처리를 통하여 성막된 c-ITO 투명전극을 15분 동안 상압 오존 공정을 통하여 계면처리함으로써 다른 변수의 영향을 배제하였으며, 표면 처리 후 spin-coating 및 droplet dropping method를 통하여 PEDOT:PSS를 코팅하여 c-ITO와 PEDOT:PSS 계면사이의 화학적 반응의 영향을 시간 경과에 따라 분석하였다. PEDOT:PSS 코팅 후 솔밴트 제거를 위해 hot plate를 이용하여 $110^{\circ}C$로 열처리되었다. Spin-coating 방법과는 달리 droplet dropping 방법을 통해 형성된 c-ITO 투명전극/PEDOT:PSS 계면에서는 spin coating에서 적용된 동일한 공정변수적용에도 불구하고 PEDOT:PSS의 산성으로 인한 ITO 투명전극 표면에서의 화학적 조성변화가(In, Sn, O의 조성의 변화) 발생됨을 x-ray photoelectron spectroscopy 결과를 통해 확인하였다. 뿐만 아니라 계면 조성반응 변화에 따른 전기적 특성 및 광학적 투과율의 열화가 발생됨을 Hall measurement 측정과 UV/Vis spectrometer 결과를 통하여 도출하였다. 본 결과를 통해 c-ITO/PEDOT:PSS 사이에서 발생되는 내산특성/계면 화학변화가 유기태양전지에서의 산화물 투명전극과 유기물 계면 열화현상에 영향을 받을 수 있음을 나타낸다.

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Two Manganese Peroxidases and a Laccase of Trametes polyzona KU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System

  • Lueangjaroenkit, Piyangkun;Teerapatsakul, Churapa;Sakka, Kazuo;Sakka, Makiko;Kimura, Tetsuya;Kunitake, Emi;Chitradon, Lerluck
    • Mycobiology
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    • v.47 no.2
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    • pp.217-229
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    • 2019
  • Two manganese peroxidases (MnPs), MnP1 and MnP2, and a laccase, Lac1, were purified from Trametes polyzona KU-RNW027. Both MnPs showed high stability in organic solvents which triggered their activities. Metal ions activated both MnPs at certain concentrations. The two MnPs and Lac1, played important roles in dye degradation and pharmaceutical products deactivation in a redox mediator-free system. They completely degraded Remazol brilliant blue (25 mg/L) in 10-30 min and showed high degradation activities to Remazol navy blue and Remazol brilliant yellow, while Lac1 could remove 75% of Remazol red. These three purified enzymes effectively deactivated tetracycline, doxycycline, amoxicillin, and ciprofloxacin. Optimal reaction conditions were $50^{\circ}C$ and pH 4.5. The two MnPs were activated by organic solvents and metal ions, indicating the efficacy of using T. polyzona KU-RNW027 for bioremediation of aromatic compounds in environments polluted with organic solvents and metal ions with no need for redox mediator supplements.

Biological Activities of Acidic Polysaccharide of Korean Red Ginseng.II.-Effects on Hyperlipidemia induced by Alcohol (홍삼 산성다당체의 생리활성 연구(II)- 알코올성 고지혈증에 미치는 영향)

  • Lee Chung Kyu;Choi Jong-Won;Kim Hyekyung;Han Yong Nam
    • Journal of Ginseng Research
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    • v.23 no.1 s.53
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    • pp.8-12
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    • 1999
  • Acidic polysaccharide of Korean red ginseng (AcPS) was subjected to alcohol-intoxicated rats to check the effects on the growth factors and lipid metabolism. Administration of AcPS showed prevent effects on the body weight, the increased contents of total cholesterol, HDL-cholesterol and neutral glyceride in the serum and total lipid, total cholesterol and neutral glyceride in the liver of alcohol intoxicated rats were significantly normalized. And all of the effects were proportionaly increased with the duration of treatments.

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Search of Optimal Contexts for Context-adaptive Coding of Stereo Parameters in Parametric Stereo of Enhanced aacPlus (Enhanced aacPlus의 Parametric Stereo에서 스테레오 파라미터의 컨텍스트 적응 코딩을 위한 최적 컨텍스트 탐색)

  • Pang, Hee-Suk
    • The Journal of the Acoustical Society of Korea
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    • v.31 no.7
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    • pp.435-440
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    • 2012
  • We propose optimal contexts for context-adaptive coding of stereo parameters in parametric stereo (PS) of enhanced aacPlus. For the quantized indexes of stereo parameters, 8 context candidates were proposed based on the index values and their combinations adjacent to a source index in the time-stereo band domain, where the time-stereo band region was further divided into 4 regions based on refresh/non-refresh frames and stereo bands. The optimal contexts for each region were proposed by experiments, which are expected to be used for context-adaptive coding of PS for improved performance.

Dipeptides Inhibit Melanin Synthesis in Mel-Ab Cells through Down-Regulation of Tyrosinase

  • Lee, Hyun-E;Kim, Eun-Hyun;Choi, Hye-Ryung;Sohn, Uy-Dong;Yun, Hye-Young;Baek, Kwang-Jin;Kwon, Nyoun-Soo;Park, Kyoung-Chan;Kim, Dong-Seok
    • The Korean Journal of Physiology and Pharmacology
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    • v.16 no.4
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    • pp.287-291
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    • 2012
  • This study investigated the effects of proline-serine (PS) and valine-serine (VS) dipeptides on melanogenesis in Mel-Ab cells. Proline-serine and VS significantly inhibited melanin synthesis in a concentration-dependent manner, though neither dipeptide directly inhibited tyrosinase activity in a cell-free system. Both PS and VS down-regulated the expression of microphthalmia-associated transcription factor (MITF) and tyrosinase. In a follow-up study also described here, the effects of these dipeptides on melanogenesis-related signal transduction were quantified. Specifically, PS and VS induced ERK phosphorylation, though they had no effect on phosphorylation of the cAMP response element binding protein (CREB). These data suggest that PS and VS inhibit melanogenesis through ERK phosphorylation and subsequent down-regulation of MITF and tyrosinase. Properties of these dipeptides are compatible with application as skin-whitening agents.

Photochemical Efficiency and psbA Gene Expression of Crinum Leaves under Natural Environmental Stress in Winter (겨울철 저온스트레스에 의한 문주란의 광합성효율과 psbA 유전자의 발현양상)

  • O, Sun Ja;Go, Seok Chan
    • Journal of Environmental Science International
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    • v.13 no.4
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    • pp.359-365
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    • 2004
  • The change of chlorophyll fluorescence parameters, O-J-I-P transients and psbA gene expression were investigated in the leaves of Crinum asiaticum var. japonicum on the natural condition in winter, in order to elucidate physiological responses of photosystem II (PS II) activity to winter stresses. The photochemical efficiencies of PS II, Fv/Fm, were significantly low in winter, contrary to its high value in summer. The values of I -qN and I-qP were lower in midday than at dawn or night both in summer and winter, although their decrease in midday was less in winter than in summer. In the O-J-I-P transients, the fluorescence intensity of J, I, P-step decreased remarkably depending on temperature drop in winter. And the D I reaction center protein of PS II decreased in late winter more than in early winter, concomitantly with relatively high content of description products of psbA gene in midday. These results indicate that low temperature in winter causes irreversible damage to PS II and subsequently leads to cell death.