• Title/Summary/Keyword: PRRs

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Effects of ozone, ultraviolet and an organic acid-based disinfectant against porcine reproductive and respiratory syndrome virus

  • Yoon, Yong-Dae;Kim, Won-Il
    • Korean Journal of Veterinary Service
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    • v.36 no.3
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    • pp.157-162
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    • 2013
  • A good level of biosecurity practice is important for efficient porcine reproductive and respiratory syndrome (PRRS) control. In the current study, disinfecting ability of ozone against PRRS virus (PRRSV) was evaluated in comparison with ultraviolet (UV) and an organic acid-based disinfectant to assess the possible use of ozone for disinfecting farm vehicles, equipments, and materials to reduce the risk of new virus introduction. For in vitro evaluation, the levels of infectious virus and viral RNA were determined on the swabs collected from the floor surface of each room treated with either ozone, UV or the disinfectant up to 30 min after contamination with 100 mL of VR2332 ($10^5\;TCID_{50}/mL$). For in vivo evaluation, 3, 4-week old, PRRS-free pigs were housed into those rooms right after the last swab collection. Then the pigs in each room were injected intramuscularly with the corresponding swab samples collected at the last time point and pooled per each room. Although ozone, UV, and the disinfectant significantly reduced the levels of PRRSV RNA contamination, ozone was most effective in removing the viral RNA. In addition, the virus collected after at least 10 min exposure to ozone failed to replicate in pigs while the virus collected after treatment with UV and the disinfectant for 30 min still replicated in pigs. Based on the results, it was concluded that ozone is more effective in inactivating PRRSV as compared with UV and the organic acid-based disinfectant.

Seroprevalence and epidemiological analysis of porcine reproductive and respiratory syndrome virus in Korea (돼지 생식기호흡증후군 바이러스의 항체분포 및 역학조사)

  • Park, Choi-kyu;Chang, Chung-ho;Kang, Yung-bae;Lee, Chang-hee;Lyoo, Young-soo;Kim, Hyun-soo
    • Korean Journal of Veterinary Research
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    • v.39 no.1
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    • pp.111-117
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    • 1999
  • A nation wide sero-epidemiological survey of porcine reproductive and respiratory syndrome(PRRS) was carried out to analyze the current status of the PRRS virus infections in the field using the indirect immunofluorescent antibody assay(IFA) with the field isolate PL96-1. Since the first report of the antibody detection to PRRSV in 1993, the prevalence of seropositive pigs has increased dramatically and the data indicate that over 21% of the pigs and around 60% of the farms showed seropositives to the PRRS virus. A slightly higher positive rate was recognized in breeders than fattenings and it might be due to the higher age at the time of testings. No significant regional differences were detected in the sero-epidemiological survey. Higher sero-positive rate in growers indicates that PRRSV infection in the field was common after weaning(around 40 days). However, the number of seropositive pigs were declined in fattening pigs. Sows showed around 26% of sero-positive rate that there is a higher chance of continuous virus circulation in the infected farms. Low rate of sero-positivity in boars(9.8%) implies that there is high demand in proper control measures to prevent virus spreading through breeding procedures such as natural or artificial insemination. Therefore it was concluded that PRRSV infection in domestic swine herds is endemic and the positive rate and economic loses will be increased by spontaneous infections in naive farms.

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Seroprevalence of porcine reproductive and respiratory syndrome (PRRS), porcine circovirus 2 (PCV-2), and mycoplasmal pneumonia of swine farms in Jeonbuk-Iksan (전북 익산지역 양돈장에서 돼지생식기호흡기 증후군, 써코바이러스-2 및 마이코프라즈마 폐렴의 항체가 조사)

  • Chu, Keun-Suk;Hyong, Sang-Gi;Lee, Ji-Young;Kim, Ji-Young;Seo, Lee-Weon;Jung, Bung-Woo
    • Korean Journal of Veterinary Service
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    • v.30 no.3
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    • pp.305-312
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    • 2007
  • The present studies was to seroepidemiological investigation with the related pneumonia due to a combination of both viral and bacterial agents. In selected herd in Jeonbuk-Iksan area of swine farms in 2006. The seroprevalence of antibody titers of PRRS, PCV-2 and mycoplasmal pneumonia were 87.2%, 69.8% and 47.4%, respectively. High seroprevalence of antibody in sow indicate that the infected sow major source of the diseases, and play an important role in circulation between production stage.