• Restorative Dentistry and Endodontics
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• 제47권4호
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• pp.41.1-41.20
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• 2022

Objectives: This study compared the clinical and radiological outcomes of regenerative endodontic procedures (REPs) using blood clots (BCs), platelet-rich plasma (PRP), and platelet-rich fibrin (PRF) through intraoral periapical radiography (IOPAR) and cone-beam computed tomography (CBCT). Materials and Methods: Forty-five single-rooted necrotic teeth with periapical pathology were randomly allocated to receive BC, PRP, or PRF as an individual scaffold. Outcomes were evaluated in 35 teeth in 23 patients with a follow-up period of 12-24 months through qualitative IOPAR scoring and quantitative CBCT measurements. Healing of periapical lesions and in immature teeth, changes in the apical foramen diameter (AFD), root wall thickness (RWT), and root length (RL) were assessed. A p value less than 0.05 was considered to indicate statistical significance. Results: All teeth were asymptomatic except 1 in the PRP group. Periapical lesion healing was seen in all except 2 teeth in the BC group and 3 in the PRP group. Both IOPAR and CBCT revealed no significant differences in bone healing or changes in AFD, RWT, and RL among the 3 groups. A positive pulp sensibility response to the cold test was seen in 2 teeth in the BC group, but none to the electric pulp test. Intracanal calcification (ICC) was evident in more teeth in the BC group than in the PRP and PRF groups, and was also significantly higher in immature teeth. Conclusions: Our results revealed that BC, PRP, and PRF have similar potential as scaffolds in REPs, and ICC may be a concern for long-term outcomes.

• 대한족부족관절학회지
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• 제26권4호
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• pp.187-191
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• 2022

The author experienced a case of autologous platelet-rich plasma (PRP) affecting the recovery of a chronic neuropathic diabetic foot ulcer combined with infection. A 65-year-aged male with uncontrolled diabetes presented with a Wagner grade 2 diabetic foot ulcer on his left forefoot of more than 2 weeks duration. Osteomyelitis, gangrene, and ischemia requiring acute intervention were absent. Although infection was controlled to a moderate degree, wound healing was unsatisfactory following surgical debridement and simple dressing. Therefore, intralesional autologous PRP injection was performed 5 times as an adjuvant regeneration therapy, and the recalcitrant ulcer healed in 3 months. Intralesional PRP injections are worthwhile as they promote wound regeneration, are evidence-based, safe, and can be easily performed in ambulatory care facilities.

• 한국식품위생안전성학회지
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• 제11권4호
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• pp.273-276
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• 1996

Platelets serve many biological functions, including a major role in the haemostatic process. But platelets also play a crucial role in the formation of arterial thrombosis, arteriosclerosis and other pathologic processes. Thus, there have been many studies to develop new antiplatelet agents from foods and plants for decades. Inthis study, inhibitory effects of the oriental onion (Allium fistulosum) on platelet aggregation were investigated using platelet rich plasma (PRP). Water extracts of oriental onion was separated into two fractions (Fraction I and Fraction II) by Sephadex G-150 column. Platelet aggregations were inhibited by total water extracts as well as Fraction I and II. IC50 value of Fraction I was much lower than that of Fraction II. Inhibitory effects of total water extracts of oriental onion on ATP release by PRP were also observed.

• 대한한방내과학회지
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• 제41권5호
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• pp.787-799
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• 2020

Objectives: Pityriasis rubra pilaris (PRP) is a rare papulosquamous disorder with unknown etiology. Studies on adults have not been conducted yet in Korean medicine. We report the progress of Korean medicine treatment for adult-type pityriasis rubra pilaris. Methods: A 62-year-old female patient was diagnosed with PRP in June 2019. After the diagnosis of PRP, retinoid treatment was continued for about 6 months, and then the drug was switched to an oral immunosuppressant. However, the patient's symptoms did not improve, but instead worsened. The patient was treated with Mihudeungsikjang-tang and acupuncture therapy. We evaluated her treatment progress based on the Dermatology Life Quality Index (DLQI), a visual analogue scale (VAS), and the changes in the patient's subjective symptoms. Results: After Korean medicine treatment, the DLQI and VAS scores improved from 18 points to 16 points and from 6 points to 4 points, respectively. The whole-body itching and scaling were reduced by 30% compared to pretreatment. The itching and pain in the neck, which had been severely symptomatic, decreased by 50%. The pain and dysesthesia in the upper and lower extremities disappeared, but the erythema still remained. Conclusions: Conventional treatments for PRP have limitations due to adverse effects and difficulty in treating refractory forms. Korean medicine treatment is worth considering as it can complement the limitations of conventional treatments, although more studies will be needed.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제27권2호
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• pp.140-150
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• 2005

Maxillary sinus lifting procedure and bone grafting are used to reconstruct atrophic maxillae. These procedure are usually followed by the placement of endosseous dental implants. Different materials and techniques can be used for sinus bone grafting. Platelets are known to contain various growth factors involved in the repair of the vasculature and tissues, and it is known that the specialized platelet secretory granules, the alpha granules, contain platelet derived growth factor(PDGF), transforming growth factor-beta(TGF-beta), insuline like growth factor-I(IGF-I), epidermoid growth factor(EGF), and others. This study was to evaluate the effect of PRP on bone formation in a sinus bone grafting. Twelve rabbits were included in this randomized, blinded, prospective pilot study. In experimental group, sinus bone grafting with autobone and platelet rich plasma. In control group, sinus bone grafting with only autobone. Rabbits were sacrificed at 2nd, 4th, 8th, 12th weeks postoperatively. Clinical and radiographic tests, histological analysis were conducted to compare both sides. In clinical examination, there in no significant difference between experimental group and control group. But, in radiographic examination, a distinct incresed in the radiopaque of the PRP experimental group at 2nd and 4th weeks. The histologic examination revealed that more new bone formation and osteoblast activity were seen in experimental group at 2nd and 4th weeks. In conclusion, PRPs action in sinus bone grafting had a capacity of increased new bone formation in a early bone healing stage.

• 한국임상수의학회지
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• 제40권2호
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• pp.93-103
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• 2023

The tendon is a dense connective tissue that connects muscle to bone and plays an essential role in joint motion. The injured tendon heals slowly owing to its low cellularity and vascularity. This study aimed to evaluate and compare the effects of regenerative injection therapy (RIT), 20 % dextrose prolotherapy (DP), and platelet-rich plasma (PRP) injections that can promote tendon healing. Twenty-one New Zealand white rabbits were divided into the control, DP, and PRP treatment groups. The superficial digital flexor tendon (SDFT) of the right hindlimb of each rabbit was used. A round defect of 2 mm was induced. Approximately 0.2 mL of 20% dextrose and autologous PRP were injected into the proximal and distal ends of the SDFT mass. Radiographic and ultrasonographic examination and cross-sectional area (CSA) calculations were performed pre-operatively and at 2, 4, and 8 weeks. The SDFT of both limbs was transected for biomechanical and histomorphometric evaluations. The SDFT of the left limb was transected for intact control. Semi-quantitative analysis was performed to evaluate the histomorphometric properties. Additional analysis was performed using H&E, Masson's trichrome, and immunohistochemical staining. The biomechanical evaluation showed that the treatment groups had higher tensile strength compared to the defect control group, while the PRP group had higher tensile strength than the DP group. On histological examination, the treatment groups appeared to be relatively closer to the remodeling phase of the healing process than the defect control group; the characteristics of the PRP group were closer to the remodeling phase than those of the DP group. The ultrasonographic examination showed different tendencies. Increased values in the CSA were observed during the early period in the treatment groups. This study suggests that PRP and DP can promote the healing of tendon injury, and these effects were superior with PRP than that with DP.

• Pediatric Infection and Vaccine
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• 제13권2호
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• pp.163-173
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• 2006

목 적 : 근래에 영아 백신의 숫자가 증가하면서 동시에 접종하는 혼합백신의 개발이 증가하고 있다. 본 연구에서는 우리나라 영아에서 b형 H. influenzae에 대한 단백 결합 백신(PRP-OMP)과 B형 간염 백신의 혼합백신인 $Comvax^{TM}$(Merck & Co.)의 면역원성 및 안전성을 평가하고자 하였다. 대상 및 방법 : 생후 직후 B형 간염 백신(유박스 $B^{TM}$ 또는 헤파박스-진$^{TM}$)을 접종받은 영아를 대상으로 $Comvax^{TM}$를 단독으로 또는 DTaP, IPV, Prevenar 등과 2, 4개월에 접종한 후, 2회 접종 전(4개월 연령) 및 2회 접종 2개월 후(6개월 연령)에 혈청 내 항 PRP 항체가와 anti-HBs를 효소면역법과 chemiluminescent microparticle immunoassay로 측정하였다. 결 과 : 총 65명(남아 35명)이 포함되었으며, 58명이 계획된 접종을 완료하였다. 1회 접종 후 항PRP 항체가의 기하 평균치는 1.96 ${\mu}g/mL$(95% CI 1.38~2.78), 2회 접종 후의 기하평균치는 10.02 ${\mu}g/mL$(95% CI 7.04~14.26)이었다. 항체가가 1.0 ${\mu}g/mL$ 이상인 비율은 1회 접종 후 63.2%(95% CI 53.75~72.65), 2회 접종 후 96.6%(95% CI 93.05~100)이었다. 한편 anti-HBs가 10 mIU/mL 이상인 영아의 비율은 4개월에 73.7%(95% CI 65.07~82.33), 6개월에 94.8%(95% CI 90.45~99.15)이었으며, 기하 평균치는 4개월에 38.32 mIU/mL(95% CI 22.42~65.51), 6개월에 101.17 mIU/mL(95% CI; 65.94~155.25)이었다. 백신 접종 후 발생한 이상반응 중 전신 증세로서 보챔(24.8%)이 가장 많았고 졸음(19.2%), 식욕부진(19.2%) 그리고 발열(7.2%) 등이 있었다. 국소 증세로는 동통이 25.6%, 발적이 19.2%, 부종이 4.8%에서 관찰되었다. 이러한 이상반응은 대부분 경증에 속하는 것으로 모두 회복되었다. 결 론 : $Comvax^{TM}$는 우리나라 영아에서 안전하고, 매우 우수한 항-PRP 항체 반응을 보였으며, 본 연구에서 B형 간염에 대해서는 아직 표준화되지 않은 방법으로 접종되었으나 비교적 우수한 항체 반응을 보여 B형 간염에 대해서도 우수한 면역원성을 지녔을 것으로 추측된다.

• 대한수의학회지
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• 제35권3호
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• pp.459-469
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• 1995

As S-nitrosothiols were proposed as nitrergic carriers in vascular and nonvascular smooth muscle, we have investigated the relaxant properties of several S-nitrosothiols in the porcine retractor penis(PRP) muscle and compared them with the effects of exogenously added NO, electrical field stimulation(EFS) of NANC nerves and sodium nitroprusside(SNP). Also the influences of oxyhemoglobin and hydroquinone on the relaxant responses were investigated. In addition, effects of NO on membrane potentials and its involvement in the generation of inhibitory junction potential(IJP) were investigated with conventional intracellular microelectrode technique. The results were summerized as follows. 1. Frequency-dependent relaxations of PRP muscle were induced by EFS to NANC nerve. Tetrodotoxin($1{\times}10^{-6}M$) abolished the relaxations of PRP muscle induced by EFS, and L-NAME(($2{\times}10^{-5}M$) and methylene blue($4{\times}10^{-5}M$) inhibited the relaxations. L-NAME-induced inhibition of the relaxations was reversed by L-arginine($1{\times}10^{-3}M$), but not by D-arginine. 2. Exogenous NO($1{\times}10^{-5}-1{\times}10^{-4}M$), sodium nitroprusside(($1{\times}10^{-7}-1{\times}10^{-4}M$) induced dose-dependent relaxations of PRP muscle. All S-nitrosothiols($1{\times}10^{-7}-1{\times}10^{-4}M$) tested relaxed the PRP muscle in dose-dependent manner and the potency order was SNAP>GSNO>CysNO>SNAC. 3. Oxyhemoglobin($5{\times}10^{-5}M$) blocked the relaxation induced by exogenous NO and inhibited EFS-, S-nitrosothiols-, and SNP-induced relaxation. 4. Hydroquinone($1{\times}10^{-4}M$) also abolished the relaxations induced by exogenous NO, and reduced the relaxations induced by S-nitrosothiols, but did not affect EFS- and SNP-induced relaxations. 5. SNP($2{\times}10^{-6}-5{\times}10^{-6}M$) relaxed muscle strips but the membrane potentials were not affected. 6. EFS with several pulses(1ms, 2Hz, 80V) produced an inhibitory junction potential(IJP) with muscle relaxation. They were abolished by TTX($2{\times}10^{-6}M$). $N^G$-nitro-$_{\small{L}}$-arginine(L-NNA, $2{\times}10^{-5}M$) abolished the muscle relaxation, but had no effect on IJP.

• Clinical and Experimental Pediatrics
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• 제50권2호
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• pp.143-150
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• 2007

목 적: 인체의 항 PRP IgG 항체를 정량적으로 측정하기 위한 방법인 표준화된 효소면역법의 타당성을 연구하는 것이 목적이다. 방 법: 인체의 항 PRP IgG 항체를 정량적으로 측정하기 위한 방법인 표준화된 효소면역법의 타당성을 연구하기 위해 특이성, 반복성, 실험실내 정밀성, 정확성, 최소 정량 한계, 및 안정성을 평가하였다. 결 과: 본 연구에서 사용한 효소면역법은 검사에 사용된 항원(HbO-HA)에 특이성을 보였으며 반복성, 실험실내 정밀성 등의 정밀성은 허용기준(반복성 : $CV{\leq}15%$, 실험실내 정밀성 : $CV{\leq}20%$)을 만족하였다. 정확성은 28개 혈청을 대상으로 한 RABA 정량결과와 효소 면역법 정량결과 비교시험에서 높은 상관계수를 보였고 첨가 회복 검사 결과 허용기준($100{\pm}20%$)을 만족하였다. 최소 정량 한계 시료 정량결과의 정밀성과 정확성은 공칭 양의 -14.7~-4.7%로 모두 허용기준(정밀성 : $CV{\leq}25%$, 정확성 : ${\pm}25%$)을 만족하였다. 안정성 중 냉 해동 안정성과 단기 온도 안정성도 모두 허용기준(${\pm}20%$ 이내)을 만족하였다. 결 론: 이상의 결과로 이화여자대학교 의과학연구소 백신효능연구센터에서 시행한 본 효소 면역법은 혈액 내에 존재하는 항 PRP IgG 항체를 정량적으로 측정하는 시험법으로 적절하였다.

• Journal of Periodontal and Implant Science
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• 제31권4호
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• pp.737-747
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• 2001

Regeneration of Periodontium with PRP does not only improve regeneration rate and density of bone but have a possibility to estimate faster healing process for soft tissue. And also, synthetic bone and xenogenic bone graft are effective on regeneration of periodontium. The purpose of this study is to evaluate the effectiveness of synthetic bone ($Biogran^{(R)}$) and xenogenic bone ($BBP^{(R)}$) grafts with the PRP technique on regeneration of periodontium. 52 Generally healthy Pt. who had pocket depth 5mm at any of 6 surfaces of the teeth were in the study at Dept. of Perio. in Dankook Dental Hospital. Open Flap was treated for 18 infra-bony pockets as control group, $Biogran^{(R)}$ with PRP was inserted for 25 infrabony pockets as first test group, and $BBP^{(R)}$ with PRP was inserted for 22 infrabony pockets as 2nd test group. Then evaluation was made after 3 and 6 months 1. 6 months after surgery, each difference of average probing pocket depth was $2.61{\pm}0.23$ for control, $3.40{\pm}0.30$ for 1st test, and $3.45{\pm}0.37$ for 2nd test group. 2. 6 months after surgery, each difference of clinical probing attachment level was $1.39{\pm}0.12$ for control, $2.88{\pm}0,24$ for 1st, and $2.86{\pm}0,27$ for 2nd test group. 3. 6 months after surgery, each difference of Maximal probing attachment level was $1.11{\pm}0.16$ for control, $3.28{\pm}0.30$ for 1st, and $3.27{\pm}0.35$ for 2nd test group. 4. There were significant differences for clinical change of each three group which were between average probing pocket depth and clinical attachment level of 3,6 months and minimal and maximal attachment level after 6 months 5. There were significant differences for average probing pocket depth which were only at control group and 2nd test group between 1 and 6months. For clinical attachment level and minimal and maximal proving attachment level, there was a significant difference after 6month of surgery. 6. There was no significant difference between two test groups for average probing depth, clinical attachment level, and minima1 and maximal probing attachment level. As the result, PRP with bone graft is very effective for regeneration of periodontium and there is no difference between xenogenic bone and synthetic bone.