• 제목/요약/키워드: PRODUCE 101

검색결과 177건 처리시간 0.027초

Simultanceous Saccharification and Fermentation of Cellulose for Lactic Acid Production

  • Yoon, Hyon-Hee
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • 제2권2호
    • /
    • pp.101-104
    • /
    • 1997
  • Lactic acid production from ${\alpha}$-cellulose by simultaneous saccharification and fermentation (SSF) was studied. The cellulose was converted in a batch SSF using cellulase enzyme Cytolase CL to produce glucose sugar and Lactobacillus delbrueckii to ferment the glucose to lactic acid. The effects of temperature, PH, yeast extract loading, and lactic acid inhibition were studied to determine the optimum conditions for the batch processing. Cellulose was converted efficiently to lactic acid, and enzymatic hydrolysis was the rate controlling step in the SSF. The highest conversion rate was obtained at 46$^{\circ}C$ and pH 5.0. The observed yield of lactic acid from ${\alpha}$-cellulose was 0.90 at 72 hours. The optimum pH of the SSF was coincident with that of enzymatic hydrolysis. The optimum temperature of the SSF was chosen as the highest temperature the microoraganism could withstand. The optimum yeast extract loading was found to be 2.5g/L. Lactic acid was observed to be inhibitory to the microorganisms' activity.

  • PDF

Lactobacillus sporgenes에 의한 젖산칼슘 생산 1. 젖산발효 (The Production of Calcium Lactate by Lactobacillus sporogenes I. Lactic acid fermentation)

  • 이계관;김영만;민경찬
    • 한국식품영양학회지
    • /
    • 제1권2호
    • /
    • pp.95-101
    • /
    • 1988
  • In order to produce lactic acid and calcium lactate very useful for foods and medical supplies, lactic acid fermentation was studied by Lactobacillus sporogenes, a spore forming lactic acid bacterium. When this bacterium was cultured aerobically in the spore forming medium, the spore forming rate was 96.0% as total cell number 20$\times$108/ml, spore number 19.2$\times$108/ml. One minute agitating every 1 hour in the flask culture, or agitation of 100rpm in the fermenter was most efficient to continue to ferment at 45$^{\circ}C$ for 4days in the fermentation medium containing 10% glucose as carbohydrate and CaCO3 as a neutralizing agent. This homofermentative lactic acid bacterium showed fermentation yield of 99.3% and more than 98.2% of the yield was L(+)-lactic acid.

  • PDF

생체 신호처리

  • 박상희
    • 대한의용생체공학회:의공학회지
    • /
    • 제10권2호
    • /
    • pp.97-101
    • /
    • 1989
  • N1 and N2 gross neural action potentials were measured from the round window of the guinea pig cochlea at the onset of the acoustic stimuli. N1-N2 audiograms were made by means of regulating stimulant intensities in order to produce constant N1-N2 potentials as criteria for different input tone pip frequencies. The lowest threshold was measured with an input tone pip I5 dB SPL in intensity and 12 KHz in frequency when the animal was in normal physiological condition. The procedure of experimental measurements is explained in detail. This experimental approach is very useful for the investigation of the Cochlear function. Both noN1inear and active functions of the Cochlea can be monitored by N1-N2 audiograms.

  • PDF

철도교량 동특성 분석을 위한 궤도형식별 모달 테스트 (Modal Tests of Railway Bridges considering the Type of Track Structures)

  • 김성일;유진영;문제우;홍성모;김종태
    • 한국철도학회:학술대회논문집
    • /
    • 한국철도학회 2008년도 춘계학술대회 논문집
    • /
    • pp.101-108
    • /
    • 2008
  • The dynamic behavior of railway bridges originates from dynamic properties of various spans and structural types. As a result, the exact estimation of dynamic properties of the railway bridge can produce the exact estimations of dynamic performances of the railway bridge. The damping ratio affects the dynamic reponses of the railway bridge in the vicinity of the critical speed seriously. Eurocode, National Annex of each European country and Japan have their own specification for the damping ratio for the estimation of dynamic performance of railway bridges. In our case, the specification for Honam high speed railways follows the Eurocode. In the present study, for the verification and regulation of the damping ratio and investigation of various dynamic properties, modal tests of various structural types are performed. In addition, for the investigation of effects of track structures on the dynamic property of the bridge, ballast track and concrete track are installed and tested.

  • PDF

$TiO_2$ 반도체 용사피막의 광전극 특성에 미치는 용사조건의 영향 (Effects of Plasma Spray Conditions on Photoelectric Properties of Plasma Sprayed $TiO_2$ Semiconductor)

  • 박정식;박경채
    • Journal of Welding and Joining
    • /
    • 제12권1호
    • /
    • pp.94-101
    • /
    • 1994
  • In this study, plasma spraying has been used to produce $TiO_2$ polycrystalline coatings from $TiO_2$ powders. The physical and chemical properties of plasma sprayed $TiO_2$ coatings depend greatly on plasma spraying conditions. The electrical resistivity, oxygen concentration, photocurrent and crystal structure of plasma sprayed $TiO_2$ coating has been studied. The results are as follows: 1. The oxygen loss and electrical conductivity of $TiO_2$ plasma sprayed coatings increased by low pressure and high amount of auxiliary gas, hydrogen in plasma spraying. 2. Oxygen loss increase electrical conductivity, and decrease photocurrent of $TiO_2$ plasma sprayed coatings. 3. Photocurrent of $TiO_2$ plasma sprayed coatings manufactured in atmospheric pressure is higher than that of low pressure.

  • PDF

TWO-LAYER MUTI-PARAMETERIZED SCHWARZ ALTERNATING METHOD

  • Kim, Sang-Bae
    • Journal of applied mathematics & informatics
    • /
    • 제9권1호
    • /
    • pp.101-124
    • /
    • 2002
  • The convergence rate of a numerical procedure barred on Schwarz Alternating Method (SAM) for solving elliptic boundary value problems (BVP's) depends on the selection of the interface conditions applied on the interior boundaries of the overlapping subdomains. It hee been observed that the Robin condition(mixed interface condition), controlled by a parameter, can optimize SAM's convergence rate. Since the convergence rate is very sensitive to the parameter, Tang[17] suggested another interface condition called over-determined interface condition. Based on the over-determined interface condition, we formulate the two-layer multi-parameterized SAM. For the SAM and the one-dimensional elliptic model BVP's, we determine analytically the optimal values of the parameters. For the two-dimensional elliptic BVP's , we also formulate the two-layer multi-parameterized SAM and suggest a choice of multi-parameter to produce good convergence rate .

Post-buckling behaviours of axially restrained steel columns in fire

  • Li, Guo-Qiang;Wang, Peijun;Hou, Hetao
    • Steel and Composite Structures
    • /
    • 제9권2호
    • /
    • pp.89-101
    • /
    • 2009
  • This paper presents a simplified model to study post-buckling behaviours of the axially restrained steel column at elevated temperatures in fire. The contribution of axial deformation to the curvature of column section is included in theoretical equations. The possible unloading at the convex side of the column when buckling occurs is considered in the stress-strain relationship of steel at elevated temperatures. Parameters that affect structural behaviours of the axial restrained column in fire are studied. The axial restraint cause an increase in the axial force before the column buckles; the buckling temperature of restrained columns will be lower than non-restrained steel columns. However, the axial force of a restrained column decreases after the column buckles with the elevation of temperatures, so make use of the post-buckling behaviour can increase the critical temperature of restrained columns. Columns with temperature gradient across the section will produce lower axial force at elevated temperatures.

Pervaporation separation of water/ethanol mixture through tubular zeolite membranes

  • Matsui, Shigetoshi;Ikeda, Madoka;Shinma, Shuji;Arano, Manabu;Mizoguchi, Kensaku;Ikeda, Shiro;Sawasaki, Toshiaki;Nakane, Takashi
    • 한국막학회:학술대회논문집
    • /
    • 한국막학회 2004년도 Proceedings of the second conference of aseanian membrane society
    • /
    • pp.98-101
    • /
    • 2004
  • Utilization of biomass resources has considerable contribution to the reduction of carbon dioxide emission. Ethanol is one of the biomass products and is used as an additive to gasoline in several countries. Conventional process to produce ethanol involves energy-intensive azeotropic distillation. Pervaporation (PV) or vapor permeation (VP) is considered to be an alternative separation process to the conventional process.(omitted)

  • PDF

Action Recognition with deep network features and dimension reduction

  • Li, Lijun;Dai, Shuling
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • 제13권2호
    • /
    • pp.832-854
    • /
    • 2019
  • Action recognition has been studied in computer vision field for years. We present an effective approach to recognize actions using a dimension reduction method, which is applied as a crucial step to reduce the dimensionality of feature descriptors after extracting features. We propose to use sparse matrix and randomized kd-tree to modify it and then propose modified Local Fisher Discriminant Analysis (mLFDA) method which greatly reduces the required memory and accelerate the standard Local Fisher Discriminant Analysis. For feature encoding, we propose a useful encoding method called mix encoding which combines Fisher vector encoding and locality-constrained linear coding to get the final video representations. In order to add more meaningful features to the process of action recognition, the convolutional neural network is utilized and combined with mix encoding to produce the deep network feature. Experimental results show that our algorithm is a competitive method on KTH dataset, HMDB51 dataset and UCF101 dataset when combining all these methods.

Agrobacterium 공동 배양을 통한 자엽절 절편 배양으로부터 멜론 형질전환체 생산 (Production of Transgenic Melon from the Cultures of Cotyledonary-Node Explant Using Agrobacterium-Mediated Transformation)

  • 조미애;송윤미;박윤옥;고석민;민성란;유장렬;이준행;최필선
    • Journal of Plant Biotechnology
    • /
    • 제32권4호
    • /
    • pp.257-262
    • /
    • 2005
  • Agrobacterium과 자엽절 공동배양으로 대두 형질전환체를 생산하였다. 멜론 (슈피VIP품종)의 자엽절 절편은 선발 마커로서 bar와 reporter로서 gus유전자가 포함된 pPTN289 또는 선발마커로서 nptII유전자와 reporter로서 gus유전자로 제작된 pPTN290벡터를 LBA4401, GV3101, EHA101에 각각 형질전환하여 공동 배양하였다. 최대 형질전환빈도(0.16%)는 EHA101 (pPTN289)균주로 공동배양한 자엽절 절편을 glufosinate가 첨가된 선발배지에서 얻을 수 있었으며, 최종적으로 glufosinate저항성과 잎 ($T_0$), 화기 ($T_0$), 종자 ($T_1$) 및 유식물체 ($T_1$)에서 GUS양성반응을 나타내는 5개체를 얻었다. Southern분석에 의하여 GUS유전자가 멜론 genomic DNA에 도입되어 있음을 확인하였다.