• 대한한방소아과학회지
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• 제28권2호
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• pp.72-87
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• 2014

Objectives This study was carried out to investigate the effect of Ganoderma lucidum extract (GLE) and microneedle therapy system (MTS) on hair growth in an alopecia model of C57BL/6N mice. Methods Five-week old mice were depilated and separated in 4 groups ; CON (50% EtOH), MXD (5% Minoxidil), MTS and GLE + MTS. The treatments were applied twice a week for 3 weeks. The hair growth was determined photographically, the hair density, thickness and length were identified by Folliscope and the weights of body and organs were measured. In dorsal skin tissue, the expression of hair growth-related gene and protein was analyzed by RT - PCR or Western blot. In addition, the hair follicles in the dermis were observed by H&E staining. Results The promotion of hair growth was observed in GLE + MTS and MTS compared to CON. The hair density, thickness and length were also improved in GLE + MTS and MTS compared to CON. The mRNA expression of TGF-${\alpha}$, TGF-${\beta}1$, IGF-1, PRL and PL and the protein expression of VEGF and IGF-1 were increased in GLE + MTS and MTS compared to CON. The hair follicles and hair root growth were improved in GLE + MTS and MTS compared to CON. In the above results, GLE + MTS were more effective than MTS. Conclusions These results suggest that GLE and MTS has a hair growth activity and can be useful for the treatment of alopecia.

• 한국가축번식학회지
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• 제22권1호
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• pp.29-34
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• 1998

본 연구는 한국재래산양 16두를 공시재료로 사용하여 분만전 12일부터 분만후 1일까지 경정맥의 혈장중 luteinizing hormone, prolactin, estradiol-17${\beta}$ 및prostaglandin F$_2$의 농도를 측정한 결과는 다음과 같다. 1, 한국재래산양에서 혈장중 luteinizing hormone외 농도는 분만전 12일부터 분만후 1일까지 0.20${\pm}$0,02~0,38${\pm}$0.04 mIU/ml의 범위로서 비교적 일정한 농도를 보였으나 prolactin의 농도는 분만전 12일부터 약간 증가하는 경향을 보였다. 2, 분만 전, 후의 estradiol-17${\beta}$의 농도는 분만전 1일에 급증하여 분만시 784.8${\pm}$77.5 pg/ml로서 최고치에 도달하였으며 분만후 1일에는 63.8${\pm}$2.8pg/ml로 감소하였다. 3. 분만 전, 후의 prostaglandin F$_2$의 농도는 분만전 12일에 323.2${\pm}$69.6 Pg/ml로 분비가 시작하여 분만 전일에 증가하였으며 분만시 1081,4${\pm}$164,9pg/ml로서 절정에 도달하여 분만후 1일에 PGF$_2$의 농도는 425.3${\pm}$60.4pg/ml로서 감소하였다. 이상의 결과는 재래산양의 분만에 있어서 estradiol-17${\beta}$의 농도에서 증가와 연관하여 prostaglandin F$_2$의 농도는 점차적으로 증가되는 것으로 추정되었다.