A pretreatment method to increase enzymatic digestibility for waste paper such as newspaper was investigated. Ash content, substrate size and printed ink were considered to be factors that affect on enzymatic hydrolysis. The effect on enzymatic digestibility of varying these factor were measured. Printed ink had the highest effect of the three factors, so a method was developed to remove the ink during pretreatment. Fist, a pretreatment process using a percolation reactor was tried. The digestibility of the substrate pretreated at 170$\^{C}$, however, was less than that of the untreated substrate because only small portion of ink was removed. Therefore, a batch type process at less than 100$\^{C}$ was devised. Of several schemes, a method using amonia-hydrogen peroxide mixture on a shaking bath proved most effective. The digestibility obtained from this method was about 85%--approximately 20% greater than the untreated substrate. This proves the pretreatment method was very effective in treating waste paper. The high digestibility obtained from this pretreatment is probably due to the effects of the hydrogen peroxide that can enhance ink removal and substrate swelling.
Evidence from the last 10 years have been suggested that melatonin mainly produce a depressant effect on the cardiac system, but we found an activating effect of melatonin on heart rate in this research. To determine the hypothesis that melatonin has dual effects on physiological behaviour of cardiac system, we investigated the effects of melatonin on heart rate in isolated rat atria and anesthetized rats. Regardless of concentration, melatonin produced bradycardia in the 84 cases of 148 experiments (57 %) and tachycardia in the 64 cases of 148 experiments (43 %). And in atrium, melatonin produced a decrease automaticity in 52 cases of 86 experiments (60 %) and increase automaticity in 40 % (34/86 cases). Also, these effects are not significnat relationship with concetration of melatonin. The melatonin-induced bradycardia in vivo was inhibited by pretreatment of atropine or bilateral cervical vagotomy. Also, in isolated atrium the melatonin-induced decrease in automaticity was inhibited by pretreatment of atropine. These melatonin-induced responses were potenitated by pretreatment of propranolol. The melatonin-induced tachycardia in vivo was inhibited by pretreatment of propranolol, nifedipine or bilateral cervical vagotomy, but not by pretreatment of atropine. The melatonin-induced incease in automaticity in isolated atrium was converted to decrease in automaticity by pretreatment of propranolol. In addition, the change in heart rate caused by adrenoceptor agonists was inhibited by pretreatment of melatonin. These results indicate that melatonin-induced bradycardia may be related to a muscarinic receptor activation and melatonin-induced tachycardia may be related to a $\beta$-adrenoceptor stimulation.
Physical and chemical barriers, caused by the close association of the main components of cellulosic biomass, hinder the hydrolysis of cellulose to fermentable sugars. Since the main goal of pretreatment is to increase the enzyme accessibility improving digestibility of cellulose, development of an effective pretreatment process has been considered to be important. In this study, SAA (Soaking in Aqueous Ammonia) was chosen as pretreatment because this is the simple and low-cost method. Rice straw of which the production is outstandingly high in domestic agriculture residues in Korea was chosen as raw material. SSA pretreatment with various reaction time of 3 h to 72 h was tested. The enzymatic hydrolysis and SSF (Simultaneous Saccharification and Fermentation) were performed at three different temperature (30, 40 and $50^{\circ}C$) to investigate performance of SSF upon various pretreatment conditions. As a result, this SAA treated-rice straw was found to have great potential for effective enzymatic hydrolysis and SSF with lower enzyme dosage at lower temperature ($30^{\circ}C$) than its conventional SSF. In SAA addition, SAA reduced fermentation time to 24 h owing to increase the initial hydrolysis rate substantially.
In this study, dilute acid pretreatment of $Liriodendron$$tulipifera$ was performed for enzymatic hydrolysis. As the pretreatment temperature was increased, enzymatic hydrolysis and enzyme adsorption yield also increased. The highest enzymatic hydrolysis yield was 57% (g/g) and enzyme adsorption was 44% (g/g). Enzymatic hydrolysis yield was determined with weight loss of pretreated biomass by enzyme, and enzyme adsorption was a percentage of enzyme weight attaching on pretreated biomass compared with input enzyme weight. When $L.$$tulipifera$ was pretreated with 1% sulfuric acid at $160^{\circ}C$ for 5 min., hemicellulose was significantly removed in pretreatment, but the lignin contents were constant. Other changes in surface morphology were detected on biomass pretreated at $160^{\circ}C$ by a field emission scanning electron microscope (FESEM). A large number of spherical shapes known as lignin droplets were observed over the entire biomass surface after pretreatment. Hemicellulose removal and morphological changes improved enzyme accessibility to cellulose by increasing cellulose exposure to enzyme. It is thus evidence that enzyme adsorption is a significant factor to understand pretreatment effectiveness.
The pharmacological actions of ambrein were investigated alone or in combination as a pretreatment with agonists (adrenaline, noradrenaline, acetylcholine, histamine, nicotine), antagonists (atropine, atenolol) and calcium channel blocker (verapamil) in vivo in anaesthetized SWR rats using blood pressure, heart rate and myocardial contractility as parameters. Ambrein in the dose range of 50-200 mg/kg to the normotensive anaesthetized rats demonstrated negative chronotropic effect and increased the myocardial contractility significantly. At the mid dose (100 mg/kg) this increase in contractile force was 36% and 44% above the normal at 30 min and 60 min intervals post-treatment, respectively. Both of the lower and high doses (50 mg/kg and 200 mg/kg) had similar effects. Furthermore, this contractile response was dose related. Also, this compound produced a considerable increase in myocardial contractility when used as a pretreatment with some agonists and antagonists. The results on blood pressure did not show a considerable change when ambrein was used alone. However, ambrein pretreatment at the dose of 100 mg/kg did not block the effects of adrenaline, noradrenaline, isoprenaline and acetylcholine on heart rate and blood pressure. On the other hand, this pretreatment attenuated the sympathoadrenal effects of nicotine significantly. Chronotropic and blood pressure changes produced by histamine were also inhibited by ambrein pretreatment. This pretreatment significantly reversed the effects of atenolol but failed to demonstrate any change in the negative chronotropic, inotropic and hypotensive responses induced by verapamil. It is concluded that ambrein induced nonselective dose dependent antagonism of the effects of some agonists and antagonists require contribution of some neuromediators. However, the positive isotropic effects of ambrein possibly involve the enhancement of slow Ca channels and/or activation of ${\beta}-adrenergic$ receptors in the heart. At this moment it is difficult to explain the exact mode of action of ambrein and the studies dealing with Ca channel blocker and adrenergic blocker followed by ambrein may help to define the factors which contribute to its positive inotropic effects.
The efficiency variation of UF(tubular)/RO(spiral wound) process using acrylic wastewater treated by photo-catalyst pretreatment and coagulant-filter-neutralization pretreatment processes were discussed wit the variation of appled pressure and temperature. Ultrafiltration tubular module using acrylic wastewater treated by photo-catalyst pretreatment and coagulant-filter-neutralization pretreatment processes was shown that COD and T-N were not highly affected with the variation of appled pressure and temperature. It was shown that removal efficiency of COD and T-N was low. Removal efficiency of TDS and turbidity with ultrafiltration tubular module was better with the acrylic wastewater by photo-catalyst pretreatment than acrylic wastewater by coagulant-filter-neutralization pretreatment. T-N and TDS were shown high removal efficiency in reverse osmosis membrane process.
Kim, Ho-Yong;Gwak, Ki-Seob;Jang, Soo-Kyeong;Ryu, Keun-Ok;Yeo, Hwanmyeong;Choi, In-Gyu
Journal of the Korean Wood Science and Technology
/
v.43
no.5
/
pp.578-590
/
2015
The present study examines the influence of slurry composting and biofiltration liquid fertilizer (SCBLF) treatment on the biomass characteristics of yellow poplar, and the optimization of organosolv pretreatment for sugar production. After SCBLF treatment, total exchangeable cation contents of yellow poplar was increased from $3.1g\;kg^{-1}$ to $4.4g\;kg^{-1}$, and as a result, biomass production of yellow poplar was also enhanced by 82.3%. Organosolv pretreatment was conducted with three independent variables: 1) reaction temperature: $133.2^{\circ}C$ to $166.8^{\circ}C$; 2) acid concentration: 0.2% to 1.8%; and 3) reaction time: 1.6 min to 18.4 min. Reaction temperature was the most significant variable in water insoluble solid (WIS) recovery rate. High overall sugar yield was attained from pretreatment conditions approximately 50% of WIS recovery rate, and the highest overall glucose yield (44.0%) was achieved from pretreatment at $140^{\circ}C$ with 1.5% acid concentration for 5 min. Consequently, 21.1% of glucose and 5.8% of xylose were produced from the organosolv pretreatment of SCBLF-treated 8-year-old yellow poplar.
The effects of chemical pretreatments on the excess sludge production in the membrane-coupled bioreactor were investigated. In addition, their effects on membrane fouling were also evaluated. Two membrane bioreactors were operated. In one reactor, a part of the mixed liquor was t reated with NaOH and ozone gas consecutively and was returned to the reactor. T he f lowrate of the chemical pretreatment stream was 1.5% of the influent flowrate. During the 200days of operation, the MLSS level in the bioreactor with mixed liquor pretreatment was maintained relatively constant at the range of 8,000 ~ 10,000$mg/{\ell}$ while it increased steadily up to 26,000 $mg/{\ell}$ in the absence of the pretreatment. Each reactor was equipped with two laboratory membrane modules where the flux for each module was 20, and 30 ${\ell}/m^2{\cdot}h$, respectively. With pretreatment, almost constant transmembrane pressure(TMP) was observed throughout the operation at the flux of 20 ${\ell}/m^2{\cdot}h$. Without pretreatment the membrane module at the same flux could also be operated at relatively stable condition. However, as the MLSS increases up to 25,000 $mg/{\ell}$, a fast TMP increase was observed. In conclusion, a complete control of excess sludge production in the membrane-coupled bioreactor was possible without significant deterioration of the treated water quality. In addition, it was shown that stable operation in terms of TMP is possible with sludge pretreatment and recirculation.
This is a study on the effect of the dentinal pretreatment method to the bond strength between dentin and glass ionomer cement. In this study, 196 human molar teeth with sound crown were used. The dentin surfaces of these teeth were exposed with wet trimmer and polished with # 800 Emory paper and teeth were divided into 7 groups according to the pretreatment agent and method. Each group has 4 subroups of the kinds of glass ionomers. The shear bond strength were measured by Instron Universal Testing machine model 1122. The data of the evaluations were then subjected to statistical analysis using one way ANOVA and the result were as follows : 1. In Durelon liquid 20 sec scrubbing & Vitrebond filling subgroup, shear bond strength was highest with measurements of 72.41(kg/$cm^2$) and in no pretreatment & Shofu lining cement filling subgroup, lowest with measurements of 4.77(kg/$cm^2$). 2. In no pretreatment group, statistical significant differences were found between the subgroups of G-C lining cement and Shofu lining cement. 3. In Ketac conditioner 20 sec scrubbing group, Vitrebond were bonded stronger than others, and in Ketac conditioner 10 sec passive contact group, it has the significant difference with other glass ionomers except G-C lining cement. 4. The subgroup og G-C dentin conditioner 20 sec scrubbing & G-C lining cement filling was bonded to dentin stronger than the subgroup of no pretreatment & Shofu lining cement. In G-C dentin conditioner groups, both of 10 sec passive contact and 20 sec scrubbing, Vitrebond has highest bond strength among the subgroups. 5. The subgroup of Durelon liquid 10 sec passive contact & G-C lining cement filling was bonded to dentin stronger than the subgroup of no pretreatment & Shofu lining cement. Also in both Durelon liquid groups, Vitrebond were bonded to dentin with the highest strength among the subgroups.
Anaerobic digestion is a popular sewage sludge (Ss) treatment method as it provides significant pollution control and energy recovery. However, the low C/N ratio and poor biodegradability of Ss necessitate pretreatment methods that improve solubilization under anaerobic conditions in addition to anaerobic co-digestion with other substrates to improve the process efficiency. In this study, three pretreatment methods, namely microwave irradiation, ultrasonication, and heat treatment, were investigated, and the corresponding improvement in methane production was assessed. Additionally, the simplex centroid design method was utilized to determine the optimum mixture ratio of food waste (Fw), livestock manure (Lm), and Ss for maximum methane yield. Microwave irradiation at 700 W for 6 min yielded the highest biodegradability (62.0%), solubilization efficiency (59.7%), and methane production (329 mL/g VS). The optimum mixture ratio following pretreatment was 61.3% pretreated Ss, 28.6% Fw, and 10.1% Lm. The optimum mixture ratio without pretreatment was 33.6% un-pretreated Ss, 46.0% Fw, and 20.4% Lm. These results indicate that the choice of pretreatment method plays an important role in efficient anaerobic digestion and can be applied in operational plants to enhance methane production. Co-digestion of Ss with Fw and Lm was also beneficial.
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