• Clinical and Experimental Reproductive Medicine
• /
• v.28 no.2
• /
• pp.79-86
• /
• 2001

Objective: Mouse pre-antral follicles require the addition of gonadotropins (Gns) to complete maturation and ovulation of oocyte and antrum formation in vitro. However, we tried examination of in vitro growth of mouse pre-antral follicles in medium without Gns and/or phygiological factors. And also, pre-antral follicles were isolated from ovaries by mechanical method. Our present studies were conducted to evaluate on the growth of follicles and intra-follicular oocytes and antrum formation in vitro of mouse pre-antral follicles in two different media. Methods: Pre-antral follicles ($91{\sim}120{\mu}m$) were isolated mechanically by fine 30G needles not using enzymes from ovaries of 3-6 week-old female ICR mice. Isolated pre-antral follicles were cultured in $20{\mu}l$ droplets of TCM (n=17; follicles: $107.8{\pm}1.58{\mu}m$; oocytes: $57.9{\pm}1.2{\mu}m$) or MEM (n=12; follicles: $109.3{\pm}2.53{\mu}m$; oocytes: $55.4{\pm}1.6{\mu}m$) under mineral oil on the 60 mm culture dish. All experimental media was supplemented with 10% FBS without Gns and/or physiological factors. Pre antral follicles were individually cultured for 8 days. Antram formation and growth of pre-antral follicles and intra-follicular oocytes were evaluated using precalibrated ocular micrometer at X200 magnifications during in vitro culture. Results were analyzed using combination of Student's t-test and Chi-square, and considered statistically significant when p<0.05. Results: Antrum formation had started in two culture media on day 2. On day 8, antrum formation had occurred in 58.3% of pre-antral follicles cultured in DMEM, but only in 23.5% of those cultured in TCM (p=0.0364). Growth of pre-antral follicles and intra-follicular oocytes were observed on day 4 and 8. On day 4, follicular diameter was similar (p=0.1338) in TCM ($119.4{\pm}2.58{\mu}m$) and MEM ($125.4{\pm}4.52{\mu}m$). However, on day 8, diameters of pre-antral follicles cultured in MEM ($168.9{\pm}17.29{\mu}m$) were significantly bigger (p=0.0248) than that in TCM ($126.7{\pm}4.28{\mu}m$). On day 4 and 8, diameters of intra-follicular oocytes were similar in TCM ($67.1{\pm}1.3$ and $72.4{\pm}0.9{\mu}m$) and MEM ($65.2{\pm}1.7$ and $73.3{\pm}1.5{\mu}m$), respectively. Conclusion: We can conform that medium without Gns and/or physiological factors can be used for in vitro antrum formation and growth of pre-antral follicles and intra-follicular oocytes in mouse. In conclusion, MEM supplemented with FBS can be used for growth in vitro of mouse pre-antral follicles isolated mechanically.

• The Mathematical Education
• /
• v.54 no.1
• /
• pp.1-11
• /
• 2015

The purpose of this study was to provide fundamental data and implication for forming desirable viewpoint of mathematics lesson critique and for improvement of expertise in lesson by compare and analyze the viewpoint of mathematics lesson critique of pre-service and in-service teachers. For the achievement of the purpose, The lesson critique materials of each 25 pre-service and in-service mathematics teachers were collected and arranged, and compare and analyze the viewpoint of mathematics lesson critique of pre-service and in-service teachers by using the standard for analyzing viewpoint of lesson critique composed of two categories, five main codes and 18 sub codes. The results of this study, both pre-service and in-service teachers have tendency for criticising lesson focusing on the practice rather than plan of lesson and both pre-service and in-service teachers showed evaluative reaction in most of critical statements and used positive ones rather than negative ones. The only pre-service teachers, though minor of them, showed non-evaluative reaction in criticising class and most of the statements were just description and explanation without any their own judgement for the scenes and activities of lessons and most of pre-service teachers have tendency to include reflection and introspection for their own class in lesson critique.

• Journal of the Korean Society for Railway
• /
• v.19 no.3
• /
• pp.288-296
• /
• 2016

A 400km/h simple catenary system was constructed as a test line in Korea. In the design stage of this system, the pre-sag was one of the engineering issues most focused on. It is known that the pre-sag improves the current collection performance in a certain band of high speed. However, the effect of pre-sag at 400km/h has not yet been established. To grasp a better pre-sag in the 400km/h catenary, we transacted the dynamic performance prediction simulation between catenary and pantograph under conditions of 0 and 1/3000 pre-sag. The level of 0 pre-sag was adapted for the 400km/h catenary design after reviewing predictions. We constituted the 1/3000 pre-sag sample section (about 1km) while constructing the 400km/h catenary test-bed (28km) of 0 pre-sag. With a HEMU-430X train, the contact forces were measured in the test-bed including the pre-sag sample section. In this paper, the predicted and measured dynamic performance values (contact forces) for 0 and 1/3000 pre-sag are described and compared. It is conclusively confirmed by analytical and experimental examination that the non pre-sag showed better dynamic (current collection) performance than that of the 1/3000 pre-sag for the 400km/h catenary system.

• Bulletin of the Korean Mathematical Society
• /
• v.52 no.1
• /
• pp.125-135
• /
• 2015

Let R be a commutative ring and U an R-module. The aim of this paper is to study the duality between U-reflexive (pre)envelopes and U-reflexive (pre)covers of R-modules.

• Honam Mathematical Journal
• /
• v.26 no.3
• /
• pp.269-280
• /
• 2004

In this paper, some new characterizations of quasi pre-$R_0$ spaces due to Tapi et al. [The Mathematics Education XXIX(3) (1995) 147] are obtained. A new notion of quasi-pre-$R_1$ spaces is defined and their properties are studied to some extent.

• Environmental Engineering Research
• /
• v.12 no.1
• /
• pp.1-7
• /
• 2007

Most of the herb residue producing from oriental medical clinics(OMC) and hospitals(OMH) is wasted in Korea. To develop of adsorbent for removing heavy metal from wastewater, the various pre-treatment methods of the herb residue were evaluated by potentiometric titration, Freundlich isotherm adsorption test and the kinetic adsorption test. The herb residue was pre-treated for increasing the adsorption capacity by cleaning with distilled water, 0.1 N HCl and 0.1 N NaOH and by heating at $370^{\circ}C$ for 30 min. It showed a typical weak acid-weak base titration curve and a short pH break like commercial activated carbon during photentiometric titration of pre-treated herb residue. The log-log plots in the Freundlich isotherm test were linear on the herb residue pre-treated with NaOH or HCl like commercial activated carbon. The adsorption capacity(qe) in the Freundlich isotherm test for $Cr^{6+}$ was 1.5 times higher in the pre-treated herb residue with HCl than in activated carbon. On the other hand the herb residue pre-treated with NaOH showed the good adsorption capacities for $Pb^{2+}$, $Cu^{2+}$ and $Cd^{2+}$ even though those adsorption capacities were lower than that of activated carbon. In kinetic test, most of heavy metals removed within the first 10 min of contact and then approached to equilibrium with increasing contact time. The removal rate of heavy metals increased with an increase of the amount of adsorbent. Likewise, the removal rates of heavy metals were higher in the herb residue pre-treated with NaOH than in that pre-treated with HCl. The adsorption preference of herb residues pre-treated with NaOH or HCl was $Pb^{2+}>Cu^{2+}$ or $Cd^{2+}>Cr^{6+}$ in the order. Conclusively, the herb residue can be used as an alternative adsorbent for the removal of heavy metals depending on pr-treatment methods.

• The Pure and Applied Mathematics
• /
• v.14 no.1 s.35
• /
• pp.15-21
• /
• 2007

We introduce the notion of pre-convergence of p-stacks and characterize the pre-interior, pre-closure, separation axioms and pre-continuity on a topological space by using pre-convergence of p-stacks. We also introduce the notion of p-precompactness and investigate its properties in terms of pre-convergence of p-stacks.

• BMB Reports
• /
• v.29 no.2
• /
• pp.175-179
• /
• 1996

Viral surface proteins are known to play an essential role in attachment of the virus particle to the host cell membrane. In case of the hepatitis B virus (HBV) several reports have described potential receptors on the target cell side, but no definite receptor protein has been isolated yet. As for the viral side, it has been suggested that the preS region of the envelope protein, especially the preS1 region, is involved in binding of HBV to the host cell. In this study, preS1 region was recombinantly expressed in the form of a maltose binding protein (MBP) fusion protein and used to identify and visualize the expression of putative HBV receptor(s) on the host cell. Using laser scanned confocal microscopy and by FACS analysis, MBP-preS1 proteins were shown to bind to the human hepatoma cell line HepG2 in a receptor-ligand specific manner. The binding kinetic of MBP-preS1 to its cellular receptor was shown to be temperature and time dependent. In cells permeabilized with Triton X-100 and treated with the fusion protein, a specific staining of the nuclear membrane could be observed. To determine the precise location of the receptor binding site within the preS1 region, several short overlapping peptides from this region were synthesized and used in a competition assay. In this way the receptor binding epitope in preS1 was revealed to be amino acid residues 27 to 51, which is in agreement with previous reports. These results confirm the significance of the preS1 region in virus attachment in general, and suggest an internalization pathway mediated by direct attachment of the viral particle to the target cell membrane.

• Journal of Korean Biological Nursing Science
• /
• v.19 no.3
• /
• pp.123-130
• /
• 2017

Purpose: This study aimed to identify factors influencing intra-operative core body temperature (CBT), and to develop a predictive model for intra-operative CBT in laparoscopic abdominal surgery. Methods: The prospective observational study involved 161 subjects, whose age, weight, and height were collected. The basal pre-operative CBT, pre-operative blood pressure, and heartbeat were measured. CBT was measured 1 hour and 2 hours after pneumoperitoneum. Results: Explanatory factors of intra-operative hypothermia (< $36^{\circ}C$) were weight (${\beta}=.361$, p< .001) and pre-operative CBT (${\beta}=.280$, p= .001) 1 hour after pneumoperitoneum (Adjusted $R^2=.198$, F= 7.56, p< .001). Weight was (${\beta}=.423$, p< .001) and pre-operative CBT was (${\beta}=.206$, p= .011) 2 hours after pneumoperitoneum (Adjusted $R^2=.177$, F= 5.93, p< .001). The researchers developed a predictive model for intra-operative CBT ($^{\circ}C$) by observing intra-operative CBT, body weight, and pre-operative CBT. The predictive model revealed that intra-operative CBT was positively correlated with body weight and pre-operative CBT. Conclusion: Influence of weight on intra-operative hypothermia increased over time from 1 hour to 2 hours after pneumoperitoneum, whereas influence of pre-operative CBT on intraoperative hypothermia decreased over time from 1 hour to 2 hours after pneumoperitoneum. The research recommends pre-warming for laparoscopic surgical patients to guard against intra-operative hypothermia.

• Journal of Korean Elementary Science Education
• /
• v.40 no.1
• /
• pp.1-12
• /
• 2021

This study investigated pre-service elementary teachers' understanding of the planetary revolution movement of Mars and their explanatory models to show how the Sun-Earth-Mars system worked. An assessment item set using five celestial maps drawn from the Stellarium was designed to probe pre-service teachers' understanding of the prograde-retrograde motion of Mars. Among 23 participants, only four showed scientifically accurate understanding of Mars movement and drawing correct explanatory models for the planetary movement. Even the pre-service teachers who construed correctly prograde and retrograde motions of Mars showed a clockwise movement model due to their intuitive perceptions of Mars movement data from the celestial maps. Pre-service teachers with poor understanding of planetary movement also showed weak explanatory models due to their limited observation or lower spatial thinking. Although the planetary motion is not an easy topic for pre-service elementary teachers, it can be argued if the alternative approach, such as using appropriate observational data of a planet and changing the frames of reference between Earth-based view and Space-based view, is employed effectively in teaching planetary motion, pre-service teachers can reach the upper level of leaning planetary motion in terms of the planet's revolution.