• Korean Journal of Microbiology
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• v.30 no.2
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• pp.141-148
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• 1992

In order to overexpress bacteriophage PRD1 DNA polymerase in E. coli cells, the 2 kb HaeII fragment was isolated from phage genomic DNA. This fragment was then cloned into pEMBL/sup ex/ 3-expression vector. A specific 57bp deletion was performed by using uracil containing ss DNA and oligonucleotide spanning each region to remove an unwanted non-coding region. After this deletion, the PRD1 DNA polymerase gene is totally under the control of the vector promoter and SD sequence. Upon heat induction, a protein with an apparent size of 68 kdal was overexpressed as an active PRD1 DNA polymerase. The expression of PRD1 DNA polymerase was about 1% of total E. coli protein.

• Korean Journal of Microbiology
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• v.29 no.4
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• pp.209-214
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• 1991

The PRD1 DNA polymerase is a small multi-functional enzyme containing conserved amino acid sequences shared by family B DNA polymerases. Thus the PRD1 DNA polymerase provides an useful model system with which to study structure-functional relationships of DNA polymerase molecules. In order to investigate the functional and structural roles of the highly conserved amino acid sequences, we have introduced three mutations into a conserved amino acid of the PRD1 DNA polymerase. Genetic complememtation study indicated that each mutation inactivated DNA polymerase catalytic activity.

• Journal of the Korean Institute of Gas
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• v.13 no.4
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• pp.33-39
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• 2009

This study is intended to experiment performance of pressure relief device and to extend the effective ways to prevent cylinders of NGV from bursting when they are exposed to local fire intensively or when they are overcharged under ambient temperature at fueling stations in summer. In the results of thermal cycling experiments, all products of three companies met the requirements for gas leakage in the qualification criteria between $82^{\circ}C$ and $-40^{\circ}C$. But the o-rings of two companies' specimens among the three companies' specimens got damaged under the accelerated conditions between $135^{\circ}C$ and $-45^{\circ}C$. It took one minute and thirty nine seconds for a glass bulb type of a thermal sensitive type PRD to activate and it took two minutes and thirty one seconds for a fusible plug type of a thermal sensitive type PRD to activate. These results indicated that a glass bulb type of a thermal sensitive type PRD was one minute faster than a fusible plug type of a thermal sensitive type PRD. Under the accelerated condition $135^{\circ}C$, the activation pressure of a pressure sensitive type PRD burst at 32.1 MPa and, under the condition of qualification criteria, it burst from 30.7 MPa to 32.1 MPa.. As a result of the experiment for performance of pressure relief device, in the case of the thermal sensitive type PRD, a glass bulb type is more effective to flame than a fusible plug type. we confirmed that the rupture pressure of a pressure sensitive type PRD could not be affected by temperature and pressure cycling.

• 기계와재료
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• v.26 no.1
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• pp.50-62
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• 2014

원전발전소에 납품되는 원전용 안전밸브의 성능은 ASME나 KEPIC와 같은 기술기준코드에 따라 설정압력, 블로우다운(blowdown), 풀리프트(Full lift), 누설(leakage), 방출용량(discharge flow rate)등과 같은 특성인자로 평가된다. 그 중에서도 방출용량은 ASME SEC III NB 7000 코드에 따라 ASME 용량인증이 요구되지만, 아직 국내에는 국제적으로 공인되니 압력방출장치 용량시험기관이 부재하여 외화유출 및 국내 PRD 기술개발에 많은 어려움이 있어왔으며, 이에 따라 원전 PRD 인증기관 개발을 통하여 세계적인 인프라 구축의 필요성이 부각되고 있다. PRD 인증기관 구축은 국내 원전 핵심기자재의 고급기술 국산화에 이바지 할 수 있고, PRD 성능 및 용량시험지원을 통하여 국내 밸브기술 경쟁력 확보하는 데 기여할 것으로 기대된다.

• The Microorganisms and Industry
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• v.16 no.3
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• pp.6-14
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• 1990

PRDI DNA polymerase is the smallest member of the family B DNA polymerase (Jung et al., 1987). This DNA polyerase is specified by bacteriophage PRDI which infects a wide variety of gram-negative bacteria(Mindich and Bamford, 1988). Because PRDI is highly amenable to genetic and biochemical manipulation, it is a convenient model system with which to study structure-function relationships of DNA polymerase molecules. To determine the functional roles of the highly conserved regions of the family B DNA polymerases, we have initiated site-directed mutagenesis with PRD1 DNA polymerase, and our results show that mutations at the conserved regions within PRD1 DNA polymerase inactivate polymerase complementing activity and catalytic activity.

• Journal of Sensor Science and Technology
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• v.21 no.5
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• pp.352-358
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• 2012

A sensor network system can be an efficient tool for healthcare telemetry for multiple users due to its power efficiency. One drawback is its limited data size. This paper proposed a real-time application of data compression/decompression method in u-Health monitoring system in order to improve the network efficiency. Our high priority was given to maintain a high quality of signal reconstruction since it is important to receive undistorted waveform. Our method consisted of down sampling coding and differential Huffman coding. Down sampling was applied based on the Nyquist-Shannon sampling theorem and signal amplitude was taken into account to increase compression rate in the differential Huffman coding. Our method was successfully tested in a ZigBee and WLAN dual network. Electrocardiogram (ECG) had an average compression ratio of 3.99 : 1 with 0.24% percentage root mean square difference (PRD). Photoplethysmogram (PPG) showed an average CR of 37.99 : 1 with 0.16% PRD. Our method produced an outstanding PRD compared to other previous reports.

• Atmosphere
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• v.24 no.2
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• pp.173-188
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• 2014

The KMA has cooperated with the Oklahoma University in USA to develop a Polarimetric Radar Data (PRD) simulator to improve the microphysical processes in Korea Local Analysis and Prediction System (KLAPS), which is critical for the utilization of PRD into Numerical Weather Prediction (NWP) field. The simulator is like a tool to convert NWP data into PRD, so it enables us to compare NWP data with PRD directly. The simulator can simulate polarimetric radar variables such as reflectivity (Z), differential reflectivity ($Z_{DR}$), specific differential phase ($K_{DP}$), and cross-correlation coefficient (${\rho}_{hv}$) with input of the Drop Size Distribution (DSD) and scattering calculation of the hydrometeors. However, the simulator is being developed based on the foreign observation data, therefore the PRD simulator development reflecting rainfall characteristics of Korea is needed. This study analyzed a potential application of the 2-Dimension Video Disdrometer (2DVD) data by calculating the raindrop axis ratio according to the rain-types to reflect Korea's rainfall characteristics into scattering module in the simulator. The 2DVD instrument measures the precipitation DSD including the fall velocity and the shape of individual raindrops. We calculated raindrop axis ratio for stratiform, convective and mixed rainfall cases after checking the accuracy of 2DVD data, which usually represent the scattering characteristics of precipitation. The raindrop axis ratio obtained from 2DVD data are compared with those from foreign database in the simulator. The calculated the dual-polarimetric radar variables from the simulator using the obtained raindrop axis ratio are also compared with in situ dual-polarimetric observation data at Bislsan (BSL). 2DVD observation data show high accuracies in the range of 0.7~4.8% compared with in situ rain gauge data which represents 2DVD data are sufficient for the use to simulator. There are small differences of axis ratio in the diameter below 1~2 mm and above 4~5 mm, which are more obvious for bigger raindrops especially for a strong convective rainfall case. These differences of raindrop axis ratio between domestic and foreign rainfall data base suggest that the potential use of disdrometer observation can develop of a PRD simulated suitable to the Korea precipitation system.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.2001-2004
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• 2008

모든 심전도 압축에서는 압축율과 신호왜곡간의 관계를 다루며 이는 매우 중요하다. 특별히 임상적인 의미를 가진다고 평가되는 5%의 Percent Root mean square Difference(PRD)값을 만족 시키면서 높은 압축율을 얻기 위한 연구는 필수적이다. 본 논문에서는 DCT를 사용하여 심전도 압축을 수행하였을 때, 심전도의 주요한 파라미터인 파형과 RRI(R-R Interval)가 압축율에 따라 어떻게 변화하는지를 평가하고 심전도의 두 가지 주요 파라미터를 진단정보의 왜곡 없이 압축할 수 있는 DCT계수 및 압축율을 도출해 내었다. 실험에는 MIT-BIH ECG Compression Test Database를 사용하였으며 DCT압축을 수행하였을 때 5 % 이하의 PRD를 확보하기 위해서는 81개 샘플에 대하여 평균 4.496 : 1, 최하 3.422 : 1 의 CR을 가지는 것을 확인할 수 있었으며, QRS를 올바르게 검출하는 범위에서의 78개의 샘플에 대하여 평균 CR은 17.3 : 1 최저 CR은 4.6512 : 1 로 나타났다. QRS 검출 한계에서의 RRI 시간왜곡은 평균 3.7149 $\pm$ 4.3147 ms로 나타났으며, 최대 시간왜곡은 13.0256 $\pm$ 14.2035 ms 로 조사되었다.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.10
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• pp.1443-1451
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• 2014

Increasing cost and scarcity of maize has stimulated the use of alternative feed sources (AFS) in the diets of cattle. In this study, we investigated the effects of partial or total replacement of maize on nutrient digestibility, growth performance, blood metabolites, and economics in Limousin crossbred feedlot cattle. Forty-five $Limousin{\times}Luxi$ crossbred bulls were randomly assigned to the three treatment groups, orthodox diet (OD; 45.0% maize), partial replacement diet (PRD; 15% maize, 67% AFS), total replacement diet (TRD; 0% maize, 100% AFS). The growth feeding trial lasted for 98 days. Dry matter intake (DMI) and average daily gain (ADG) were recorded. The digestion trial was carried out after the end of the growth trial. Total faeces and feed samples were measured daily. Digestibilities of dry matter (DM) and organic matter (OM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) were calculated. After the feeding trial, blood metabolites were measured in 12 animals from each group. Initial and final body weights did not differ significantly among treatment groups (p>0.05). The ADG and DMI were 1.72 and 8.66, 1.60 and 9.10, and 1.40 and 9.11 kg/d for OD, PRD, and TRD, respectively. The PRD and TRD exhibited lower ADG (p<0.01) and higher DMI (p<0.01) than OD. The DMI (%body weight) was comparable between groups (p>0.5). Feed efficiency of PRD and TRD were lower than OD (p<0.01). The DM digestibility decreased with reduced level of maize (p = 0.10), OM digestibility was higher in OD (p<0.05), and CP, NDF and ADF digestibilities were similar for all groups (p>0.05). Blood urea nitrogen (mg/dL) in PRD and TRD was higher than OD (p<0.01), while other blood parameters did not differ significantly. Feed costs ($/head/d) were 1.49, 0.98, and 0.72 for OD, PRD, and TRD, respectively (p<0.01). Feed costs per kg gain ($) were significantly lower for PRD (0.63) and TRD (0.54) than OD (0.89; p<0.01). Overall profit ($/head) and daily profit ($/head/d) did not differ significantly between treatments (p>0.05), although TRD showed the highest economic benefits overall (p<0.01). While a traditional diet maximized the growth rate, partial or total replacement of dietary maize with AFS proved economically feasible due to their lower costs and comparable nutrient digestibilities of DM, CP, NDF, and ADF. Partial replacement may prove economically competitive in the current situation of China.

• Speech Sciences
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• v.10 no.4
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• pp.229-239
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• 2003

The purposes of this study are; to investigate the swallowing mechanism of stroke patients with different consistencies through the modified barium swallowing(MBS); and to establish preliminary data on the differences in swallowing durations. 4 different kinds of consistency-water, nectar, pudding, and crackers-were given to 6 stroke patients and their swallowing durations recorded through the fluoroscopy were measured in 1/100 second units. The results show that first swallowing time(FST), the oral preparatory duration(OPD), and the pharyngeal response duration(PRD) were delayed in swallowing thicker consistencies. However, water exhibited delayed oral and pharyngeal phase relative to its consistency and 50% of subjects showed oral loss of water. The relationship between consistencies and swallowing durations and the clinical issues on the stroke population were discussed.