• Title/Summary/Keyword: PR-1

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Induction by Salicylic Acid and Characterization of PR-Proteinases from Bulbs of Lilium formosanum Wallace (흰나리 인편으로부터 PR-Proteinase의 유도 및 특성 규명)

  • 오순자
    • Korean Journal of Plant Resources
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    • v.11 no.2
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    • pp.195-201
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    • 1998
  • Some morphological character were surveyed and PR-proteinases were induced and characterized from Lilium formosanum Wallace endeimc to Cheju island . Its flower characters were similar to those of white trumpet lilies(Lilium longiflorum Thunb) although its flowering period was later than that of white trumpet lilies and it hadd a wide range of variation among individuals. Six PR-proteinases(II-2, III-1, III-2, IV-1, IV-2 and V) were induced from bulbs by 2.5mM salicylic acid and almost excreted into the intercellular spaces. These PR-proteinases were strongly activated by Ca 2+, , whereas they were strongly inhibited by Cu2+ Co2+ and Fe2+ . Three PR proteinases(II-2, IV-1 and IV-2) were strongly inhibited by 1, 10 -phenanthroline, indicating that these enzymes are metallo-proteinases. Three PR-proteinases(III-1, III-2 and V) had a high sensitivity to PMSF and required $\beta$-mercaptoethanol for their activities. These results indicate that these proteinases are cysteine proteinases.

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Studies on a PR4 Gene for Breeding Disease Resistant Forage Crops (내병성 목초 품종개량을 위한 PR4 유전자의 연구)

  • Cha, Joon-Yung;Ermawati, Netty;Jung, Min-Hee;Kim, Ki-Yong;Son, Dae-Young
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.4
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    • pp.241-248
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    • 2007
  • Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. By using mRNA differential display, we isolated a cytokinine-inducible cDNA encoding pathogenesis-related (PR) 4 from Arabidopsis amp1 mutant. The full-length PR4 cDNA, designated AtPR4, contains an open reading frame of 212 amino acids with calculated molecular mass of 22,900 Da and isoelectric point (pI) of 7.89. Genomic DNA blotting showed that the Arabidopsis genome has one copy of AtPR4. AtPR4 mRNA was induced by cytokinin and NaCl, but decreased by SA or JA treatment. PR proteins are induced in response to pathogen attack. Thus the AtPR4 gene isolated in this study may be a useful candidate for genetic engineering of forage crops for increased tolerance against pathogen.

Enable Secondary User Spectrum Access in Cooperative Communication Scheme with Rayleigh Fading Channel (레일리 페이딩 채널에서 2차 사용자 스펙트럼 접근이 가능한 협력통신 기법)

  • Kim, Lyum;Kong, Hyung-Yun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.10 no.5
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    • pp.113-119
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    • 2010
  • In this paper, the considered cognitive radio system has a pair of PT-PR and a pair of ST-SR. In first time slot(1 Phase), PT broadcast signal $x_p$ to PR, ST and SR. Then, each receivers(PR, ST, SR) decode received signal $x_p$. In second time slot(2 Phase), ST combine decoded signal ${x_p}^{\prime}$ and signal $x_s$, and it broadcast combined signal to PR and SR. PR and SR decode combined signal. At this time, PR can achieve diversity gain, due to using MRC, it combine 'received and decoded signal ${x_p}^{\prime}$ at 1 phase' and 'detected signal $x_p$ at 2 phase'. SR use linear combining technique and it can obtain $x_s$.

A Study on the Luminescent Characteristics of YPO4:Pr3+ Phosphor by the Content Ratio of Pr6O11 and Calcination Temperature (Pr6O11의 함량 및 열처리 조건에 따른 YPO4:Pr3+ 형광체의 발광 특성 연구)

  • Min Jun Kim;Seong Eui Lee
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.37 no.1
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    • pp.68-73
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    • 2024
  • In this study, the praseodymium-doped yttrium phosphate (YPO4:Pr3+) powder, which is well known for its high luminescent efficiency, and long life in the UV range, was synthesized with various content ratios of Pr6O11 and calcination temperature. Crystal structure and luminescent properties of various phosphor powders based on different concentrations and calcination conditions were characterized by XRD (X-Ray Diffraction) and PL (photoluminescence) spectrometers. From the XRD analysis, the structure of YPO4:Pr3+ which is calcinated at 1,200℃ was stable tetragonal phase and crystal size was calculated about 25 nm by Scherrer equation. PL emission of YPO4:Pr3+ with a different content ratio of Pr6O11 by excitation λexc=250 nm shows that 0.75 mol% phosphor powder has maximum PL intensity and PL decreases with the increase of the ratio of Pr6O11 up to 1.25 mol% which is caused by changes of crystallinity of phosphor powders. With increasing dopant ratio, photo-luminescence Emission decreases due to Concentration quenching, which is commonly observed in phosphors. Currently, 0.75 mol% is considered the optimal doping concentration. A hybrid ultraviolet-emitting device incorporating YPO4:Pr3+ fluorescent material with plasma discharge was fabricated to enhance UV germicidal effects while minimizing ozone generation. UV emission from the plasma discharge device was shown at about 200 nm and 350 nm which caused additional emission of the regions of 250 nm, 315 nm, and 370 nm from the YPO4:Pr3+ phosphor.

A Study of Gene Frequency of Proline-rich Protein, Double-band Protein and Pa Protien in Patotic Saliva according to Family Name of Korean People (한국인 성씨에 따른 이하선 탕개 내 Pr,Db,Pa의 유전자빈도에 관한 연구)

  • Chong-Youl Kim
    • Journal of Oral Medicine and Pain
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    • v.15 no.1
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    • pp.55-60
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    • 1991
  • After dividing 372 Korean people of 47 different family names into 307 people of 28 indigenous family name groups and 65 people of 19 immigrated family name groups and investigating Pr. Db, Pa gene frequency of each family name groups based on phenotype of parotid saliva character the author have got following conclusions. 1. The gene frequencies of indigenous family name groups were Pr1=0.686, Pr2=0.314, Pr gene frequencies of immigrated family name groups were Pr1=0.7, Pr2=0.3. 2. The gene frequencies of indigenous family name groups were Db==0.021, Db-=0.979, Pr gene frequencies of immigrated family name groups were Db+=0.023, Db-=0.977. 3. The gene frequencies of indigenous family name groups were Pa+=0.248, Pa-=0.752, Pr gene frequencies of immigrated family name groups were Pa+=0.206, Pa-=0.794. 4. The Pr gene frequencies of immigrated family name groups were in the middle of those of Chinese people and indigenous people groups. 5. There was no significant difference of Db gene frequencies between indigenous and immigrated family name groups. 6. Pa gene frequencies of immigrated family name groups were similar to those of Chinese people.

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The Effect of Pr Additive on Magnetostriction of Amorphous Fe-B Alloy (Pr 첨가가 Fe-B 비정질합금의 자왜에 미치는 영향)

  • 조용수;김윤배;김창석;김택기
    • Journal of the Korean Magnetics Society
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    • v.4 no.2
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    • pp.126-129
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    • 1994
  • In order to invetigate the effect of Pr additive on the magnetostriction of amorphous Fe-B alloys, amorphous $Fe_{86-x}B_{14}Pr_{x}(2{\leq}x{\leq}8\;at.%)$ alloys were prepared by a rapid solidification process. As the Pr content increased in the as-prepared amorphous $Fe_{84}B_{14}Pr_{2}$ alloy annealed at $300^{\circ}C$ for 2 hr increased to 70 ppm. Ac power loss and permeability$(f=50\;kHz,\;B_{m}=0.1\;T)$ of the annealed amorphous $Fe_{84}B_{14}Pr_{2}$ alloy were 15 W/kg and $5.5{\times}10^{3}$, respectively.

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Preparation and Luminescent Properties of (Sr1Ca)TiO3:Pr1Al Phosphors ((Sr1Ca)TiO3:Pr1Al 형광체의 제조와 발광특성)

  • Park Chang-Sub;Lee Jeng-Un;Yu Il
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.5
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    • pp.422-426
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    • 2006
  • [ $Sr_xCa_{(1-x)}TiO_3$ ] red phosphors doped with Pr(0.13 mol%) and Al(0.23 mol%) were synthesized by solid state reaction method. Change of crystal structure occurred in $Sr_xCa_{(1-x)}TiO_3:Pr,Al$ phosphors with increasing value of x. Green and red luminescence were observed from $SrTiO_3:Pr,Al$ phosphors at low temperature. However, only red luminescence in the case of $CaTiO_3:Pr,Al$ phosphors was measured at low temperature. The main cause of green luminescence was explained by the bandgap reduction.

Effects of Ethylene Precursor, Auxin and Methyl Jasmonate on the Aerenchyma Formation in the Primary Root of Maize (Zea mays) (옥수수(Zea mays) 원뿌리의 통기조직 발달에 미치는 에틸렌 전구체, 옥신, 메틸자스몬산의 효과)

  • Ho, Jongyoon;Maeng, Sohyun;Park, Woong June
    • Journal of Life Science
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    • v.25 no.1
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    • pp.37-43
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    • 2015
  • We have investigated the effects of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), indole-3-acetic acid (IAA) and methyl jasmonate (MeJA) on the development of aerenchyma in the primary root of maize (Zea mays). Because plant hormones affected the longitudinal organization of the primary root, we need an indicator to direct the positions for comparison between control and hormone-treated roots. Therefore, the zones of the maize primary root were categorized as PR25, PR50 and PR75, where each value indicates the relative position between the root tip (PR0) and the base (PR100). Aerenchyma was not observed at PR25 and PR50 and rarely found at PR75 in the cortex of control roots. The aerenchymal area at PR75 increased in the presence of the ethylene precursor ACC or a natural auxin IAA. On the other hand, MeJA differentially acted on non-submerged and submerged roots. Exogenously applied MeJA suppressed the aerenchyma formation in non-submerged roots. When the primary root was submerged, aerenchymal area expanded prominently. The submergence-induced aerenchyma formation was amplified with MeJA. Lateral root primordia have been known to inhibit aerenchymal death of surrounding cells. All the three hormones stimulating aerenchyma formation as described above did not restore the inhibition caused by lateral root primordia, suggesting that the inhibitory step regulated by lateral root primordia can be located after hormonal signaling steps.

Correlation between pr1 and pr2 Gene Content and Virulence in Metarhizium anisopliae Strains

  • Rosas-Garcia, Ninfa M.;Avalos-de-Leon, Osvaldo;Villegas-Mendoza, Jesus M.;Mireles-Martinez, Maribel;Barboza-Corona, J.E.;Castaneda-Ramirez, J.C.
    • Journal of Microbiology and Biotechnology
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    • v.24 no.11
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    • pp.1495-1502
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    • 2014
  • Metarhizium anisopliae is a widely studied model to understand the virulence factors that participate in pathogenicity. Proteases such as subtilisin-like enzymes (Pr1) and trypsin-like enzymes (Pr2) are considered important factors for insect cuticle degradation. In four M. anisopliae strains (798, 6342, 6345, and 6347), the presence of pr1 and pr2 genes, as well as the enzymatic activity of these genes, was correlated with their virulence against two different insect pests. The 11 pr1 genes (A, B, C, D, E, F, G, H, I, J, and K) and pr2 gene were found in all strains. The activity of individual Pr1 and Pr2 proteases exhibited variation in time (24, 48, 72, and 96 h) and in the presence or absence of chitin as the inductor. The highest Pr1 enzymatic activity was shown by strain 798 at 48 h with chitin. The highest Pr2 enzymatic activity was exhibited by the 6342 and 6347 strains, both grown with chitin at 24 and 48 h, respectively. Highest mortality on S. exigua was caused by strain 6342 at 48 h, and strains 6342, 6345, and 6347 caused the highest mortality 7 days later. Mortality on Prosapia reached 30% without variation. The presence of subtilisin and trypsin genes and the activity of these proteases in M. anisopliae strains cannot be associated with the virulence against the two insect pests. Probably, subtilisin and trypsin enzyme production is not a vital factor for pathogenicity, but its contribution is important to the pathogenicity process.

Functional Characterization of PR-1 Protein, β-1,3-Glucanase and Chitinase Genes During Defense Response to Biotic and Abiotic Stresses in Capsicum annuum

  • Hong, Jeum-Kyu;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • v.21 no.3
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    • pp.195-206
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    • 2005
  • Spatial and temporal expression of pathogenesis-related (PR) gene and proteins has been recognized as inducible defense response in pepper plants. Gene expression and/or protein accumulation of PR-1, $\beta-1,3-glucanase$ and chitinase was predominantly found in pepper plants during the inoculations by Xanthomonas campestris pv. vesicatoria, Phytophthora capsici and Colletotrichum coccodes. PR-1 and chitinase genes were also induced in pepper plants in response to environmental stresses, such as high salinity and drought. PR-1 and chitinase gene expressions by biotic and abiotic stresses were regulated by their own promoter regions containing several stress-related cis-acting elements. Overexpression of pepper PR-1 or chitinase genes in heterogeneous transgenic plants showed enhanced disease resistance as well as environmental stress tolerances. In this review, we focused on the putative function of pepper PR-1, $\beta-1,3-glucanase$ and chitinase proteins and/or genes at the biochemical, molecular and cytological aspects.