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http://dx.doi.org/10.5333/KGFS.2007.27.4.241

Studies on a PR4 Gene for Breeding Disease Resistant Forage Crops  

Cha, Joon-Yung (Division of Applied Life Science (BK21 program), PMBBRC, and EBNCRC, Gyeongsang National University)
Ermawati, Netty (Division of Applied Life Science (BK21 program), PMBBRC, and EBNCRC, Gyeongsang National University)
Jung, Min-Hee (Division of Applied Life Science (BK21 program), PMBBRC, and EBNCRC, Gyeongsang National University)
Kim, Ki-Yong (National Institute of Animal Science)
Son, Dae-Young (Division of Applied Life Science (BK21 program), PMBBRC, and EBNCRC, Gyeongsang National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.27, no.4, 2007 , pp. 241-248 More about this Journal
Abstract
Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. By using mRNA differential display, we isolated a cytokinine-inducible cDNA encoding pathogenesis-related (PR) 4 from Arabidopsis amp1 mutant. The full-length PR4 cDNA, designated AtPR4, contains an open reading frame of 212 amino acids with calculated molecular mass of 22,900 Da and isoelectric point (pI) of 7.89. Genomic DNA blotting showed that the Arabidopsis genome has one copy of AtPR4. AtPR4 mRNA was induced by cytokinin and NaCl, but decreased by SA or JA treatment. PR proteins are induced in response to pathogen attack. Thus the AtPR4 gene isolated in this study may be a useful candidate for genetic engineering of forage crops for increased tolerance against pathogen.
Keywords
AtPR4; Cytokinin; amp1 mutant; Disease resistance;
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