• 미생물학회지
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• 제30권6호
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• pp.472-478
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• 1992

HTLV-1 이 pol 유전자산물을 합성하기 위해서는 genome-size mRNA 를 번역해 나아가는 ribosome 이 -1 방향으로 두차례 frame 을 바꾸어야 한다. 우리는 단 한차계의 frameshifting 만으로도 많은 양의 Gag-Pro-Pol polyprotein 의 합성어 가능하도록 gag 와 pro 유전자의 frame 을 연결시킨 mutant RNA 를 제작하였다. 이 돌연변이를 이용하여 ribosome 의 shift site 하류영역내에 형성이 예상되는 RNA 의 이차구조 또는 삼차 구조가 -1 frameshifting 을 결정하는 인자로서 작용하는지의 여부를 조사하였다. 결손변이주의를 해석한 결과 pro-pol 중첩영역에서 효율적으로 frameshifting 이 일어나기 위해서는 stem-loop 가 필수적으로 형성되어야 하지만 pseudoknot 의 형성은 그다지 중요하지 않다는 사실을 알았다.

• 대한바이러스학회지
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• 제30권2호
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• pp.131-139
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• 2000

Bovine leukemia virus (BLV) is an etiological agent of chronic diseases in cows worldwide. The BLV is one of retroviruses that contain a multi-functional enzyme, reverse transcriptase produced from the pol gene in its genome. We have sequenced some regions in the pol gene of BLV proviruses found in the Southern province of Korea from samples that turned out to be BL V positives by a PCR analysis. On the 5' side of the BLV pol gene (polymerase region), it was found that there were four leucines located at every 7 amino acids. They can form a leucine zipper motif that was not same as the pol gene of Japanese BLV isolate. The sequencing result of the proviral pol gene in Korean-type BLV also revealed some mutations leading to amino acid changes such as $CCT(Pro){\to}CTC(Leu)$, $AAT(Asn){\to}AAA(Lys)$, and non-sensible variations i.e., $TCT(Ser){\to}TCC(Ser)$, $ATT(Ile){\to}ATC(I1e)$ and $ACG(Thr){\to}ACA(Thr)$. On the 3' side of the pol gene (integrase region), some nucleotide sequences were mutated and led to amino acid changes. Among them, a mutation, $GAA(Glu){\to}GAC(Asp)$ occurred in many Korean-type BLV proviruses was very interesting because the amino acid was regarded as one of the most conserved amino acids in the retroviral integrase. It was also notable that the mutation on any leucine residue did not occur, in spite of its frequent appearance.

• Journal of Microbiology and Biotechnology
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• 제8권2호
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• pp.178-181
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• 1998

An exopolysaccharide, designated as A49-Pol, was produced from Bacillus polymyxa KCTC 8648P in nitrogen sufficient conditions. The viscosity of the culture broth increased up to $2\times10^4$cP in 38 hours of culture and then decreased to $1.5\times10^4$CP at 48 hours. The $1.0\%$ (w/v) solution of purified A49-Pol represented pseudoplasticity with a viscosity of $2.7\times10^4$cP which was two times higher than xanthan at the same concentration. The viscosity of the A49-Pol solution was also greatly affected by its concentration in comparison with the xanthan solution. The viscosity of $1.0\%$ A49-Pol solution was 930-fold higher than its $0.2\%$ solution, whereas the corresponding viscosities of xanthan solution experienced only l7-fold difference. The viscosity was observed to be maximum at pH 7.0 in both A49-Pol and xanthan solutions, and gradually decreased as the pHs of the polysaccharide solutions went to acidic or alkaline regions. The viscosity of A49-Pol solution was very sensitive to temperature compared to xanthan and decreased with increasing temperature. The viscosity of $0.6\%$ solution of A49-Pol was 8,100 cP at $10^{\circ}C$ and 55 cP at $50^{\circ}C$. The viscosity was also affected by the presence of surfactants such as Span 20 and Triton X-l00 ; with $0.5\%$ Triton X-l00 (v/v), the viscosity of A49-Pol solution increased by $50\%$.

• 동의신경정신과학회지
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• 제24권3호
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• pp.271-280
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• 2013

Objectives : The development of natural drugs with antidepressant effects is important and needed. This study was performed to investigate the antidepressant-like effects of the distilled water extract of Portulaca oleracea L. (POL) in a mouse model and to investigate the role of ${\alpha}$-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors in producing these antidepressant-like effects. Methods : The forced swim test (FST) and tail suspension test (TST) were used to investigate the behavioral anti-depressive-like effects of POL in mice. Additional behavioral experiments with 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione, an AMPA receptor antagonist, were undertaken to determine the involvement of the antidepressant-like properties of POL in AMPA receptor throughput. Results : Oral administration of the POL extract (100 mg/kg) 1 h prior to testing significantly reduced the immobility times in the FST and TST. The antidepressant-like effects of the POL extract were not increased in a dose-dependent manner. Pre-treatment with NBQX significantly attenuated the reduction in immobility time induced by the POL extract in the FST. Conclusions : The distilled water extract of POL has antidepressant-like effects, which may be related to AMPA receptor. Pre-treatment with NBQX significantly attenuates the reduction in immobility time induced by the POL extract in the FST.

• BMB Reports
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• 제32권5호
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• pp.492-496
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• 1999

We have investigated the biochemical properties of the herpes simplex virus type 1 (HSV-1) DNA polymerase without the UL42 protein (Pol), purified from insect cells infected with a recombinant baculovirus containing the UL30 gene. BSA and DTT have inhibitory effects on dAMP incorporation. Pol showed a greater turnover rate of steady-state single nucleotide incorporation at 12 mM $MgCl_2$ than at 2 mM $MgCl_2$. However, it showed a greater processivity of DNA synthesis at lower $MgCl_2$ concentration (1 mM, 2 mM) than at a higher $MgCl_2$ concentration (12.5 mM). These results are consistent with a slow DNA dissociation at lower $MgCl_2$ concentrations. Pol does not incorporate a correct nucleotide into the primer with an incorrect nucleotide at the end; instead, it preferentially excises the incorrect nucleotide at the 3' end of the primer. Pol has DNA polymerase activity at pHs 6.5 and 7.5 but little at pHs 5.5, 8.5, and 9.5. It has exonuclease activity at pHs 6.5, 7.5, and 8.5 but little at pHs 4.5, 5.5, and 9.5. The finding that Pol has exonuclease activity but not DNA polymerase at pH 8.5 suggests that DNA binds to Pol, but deoxynucleotide binding or incorporation does not occur at pH 8.5.

• 한국산학기술학회논문지
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• 제21권7호
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• pp.277-284
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• 2020

본 실험은 모돈 사료 내 Portulaca oleracea L.(POL)의 첨가가 번식성적, 혈액성상, 면역반응 및 분 중 미생물에 미치는 영향을 구명하기 위해 수행하였다. 본 실험을 위해 총 20두의 모돈(Landrace×Yorkshire; 229.2±1.15 kg)을 공시하였으며, 개시체중에 기반하여, 2처리 10반복, 반복당 1두씩 완전임의 배치하였다. 실험 처리구는 옥수수-대두박 기초사료를 대조구로 하여 기초사료 내 POL을 0.05% 첨가한 처리구가 포함되었으며, 분만 직후부터 포유기간 동안 수행하였다. 사료 내 POL을 첨가하였을 때, 모돈의 체중, 등지방두께 및 번식성적에서는 유의적인 차이가 나타나지 않았다(p>0.05). 혈액성상에서 사료 내 POL의 첨가하였을 때, 모돈의 림프구가 유의적으로 감소하는 것으로 나타났다(p<0.05). 또한, 면역반응에서 사료 내 POL을 첨가하였을 때 포유모돈의 혈 중 IL-1β의 수치는 유의적으로 감소하였으며(p<0.05), IgG는 증가하는 경향이 관찰되었다(p=0.051). 분 중 미생물에서는 POL 처리구에서 포유모돈의 coliforms이 감소하는 경향을 확인하였다(p=0.063). 본 실험의 결과들을 종합해보면, 사료 내 POL의 첨가는 번식성적에 부정적인 영향을 미치지 않으며, 모돈의 혈액성상, 면역반응 및 분 중 미생물에 긍정적인 효과를 미치는 것을 시사한다.

• 청정기술
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• 제25권1호
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• pp.7-13
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• 2019

일련의 dimethylformamide (DMF) 기반 폴리우레탄은 메틸렌 디 페닐 디 이소시아네이트(MDI) 1,4-부탄 디올 및 바이오 폴리올(1,3-프로판 디올: B-POL)에 기초한 폴리 트리 메틸렌 에테르 글리콜/폴리 에스터 폴리올(1,4-부탄디올: H-PET)으로 부터 합성하였다. 본 연구에서는 바이오 폴리올(B-POL)/폴리에스터 폴리올(H-PET)의 조성이 폴리우레탄의 물성에 미치는 영향을 조사하였다. B-POL/H-PET 혼합물의 B-POL 함량이 증가할수록 폴리우레탄의 소프트 세그먼트의 유리전이온도(Tgs)와 인장 강도는 감소하는 반면, 파괴 신도 및 인열 강도는 증가하였다. 한편 합성된 DMF 기반 폴리우레탄을 이용하여 습식공정으로 인조피혁을 제조하였다. 인조피혁에 형성된 다공성 셀의 평균 크기 및 밀도(단위부피당 셀의 수)에 미치는 B-POL/H-PET 조성의 영향의 차이는 거의 없는 것을 알 수 있었다. 이러한 결과들로부터 바이오 폴리올 기반 폴리우레탄을 습식공정으로 제조되는 인조피혁용으로 사용하는데 별 문제가 없음을 알 수 있었다.

• 미생물학회지
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• 제30권2호
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• pp.134-140
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• 1992

Teh $polA^{+}$ gene can be transducted in a multicopy mini-Mu plasmid, but not cloned because the product of this gene is lethal when overproduced. Although, we obtained one surviving cell, in which the ColEl-derived mini-Mu plasmid suffered a spontaneous deletion exactly at the region where the $polA^{+}$ gene was cloned. The $PolA^{+}$ unstream flanking sequence containing the promoter and pribnow-box was delected in vivo ; consequently this gene is not able to be expressed.

• 한국전자통신학회논문지
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• 제11권1호
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• pp.81-86
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• 2016

300년 전에 발표한 fractional calculus의 개념인 fractional 미분 방정식을 제어공학, 수학, 물리학 등에 적용하고자 하는 노력이 지속되고 있다. 본 논문에서는 Van der Pol 방정식으로 표현되는 동적 방정식을 정수 차수와 실수 차수를 가진 fractional 차수로 표현하고 실수 차수의 값을 변화시켜 가면서 시계열 데이터와 위상공간으로 정수 차수와 실수 차수의 비교를 수행한다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.13-18
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• 2001

The role of polyhedrin in the polyhedra production in baculovirus Autograha californica Nucelopolyhedro-sisvirus (AcNPV) was studied by over-expression of AcNPV polyhedrin or heterologous polyhedrin from Bombyx mori (Bm) NPV or Spodoptera exigua (Se) NPV. The transfer vectors containing additional polyhedrin from AcNPV, BmNPV, or SeNPV were constructed and cotransfected with bacmid bApGOZA into Sf9 cells. The resulting recombinants, designated as vApAcPol, vApBmPol, and vApSePol were tonstructed, and the polyhedra production of the recombinant was characterized. All of the recombinants produced polyhedra in the nucleus, and the polyhedrin was over-expressed. Among three recombinants, vApAcPol and vApBmPol were discriminated by their larger polyhedra size than that of wild type AcNPV, and vApSePol also produced larger polyhedra than wild type SeNPV polyhedra.