• The Korean Journal of Nuclear Medicine
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• v.26 no.1
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• pp.1-7
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• 1992

To elucidate alteration of purine nucleoside phosphorylase (PNP) activity of peripheral lymphocytes and helper/inducer and suppressor/cytototxic T cells in patients with thyroid tumors, the author examined PNP activity, and $CD4^+\;and\;CD8^+$ cells of peripheral blood in 20 cases of simple goiter, 9 cases of thyroid adenoma and 20 cases of thyroid cancer as well as 11 cases of adult healthy subjects as control. Diagnoses were established on the basis of commonly accepted clinical and biochemical criteria in simple goiter and were confirmed histopathologically in thyroid adenoma and cancer. All blood was obtained from veins of the patients and control subjects in Pusan National University Hospital during the period of January to August, 1991. The results obtained were summarized as follows: 1) The PNP activity was significantly decreased or tended to be decreased in thyroid adenomas and cancers as compared with control subjects and simple goiters. 2) The percentage of CD8 cells was significantly decreased or tended to be decreased in thyroid cancers as compared with simple goiters, thyroid adenomas and control subjects. 3) The CD4/CD8 ratio was significantly increased or tended to be increased in thyroid cancer as compared with simple goiters, thyroid adenomas and control subjects. On the basis of the results, it can be suggested that the immunodysfunction in thyroid cancer may be due to decreased suppressor/cytotoxic T cells, and the estimation of PNP activity of peripheral lymphocyte is a helpful test in detecting the immune status in thyroid tumors.

• BMB Reports
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• v.34 no.1
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• pp.21-27
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• 2001

The structural stability of brain pyrydoxine-5'-phosphate (PNP) oxidase and the catalytic properties of the monomeric species were investigated. The unfolding of brain pyridoxine-5'-phosphate (PNP) oxidase by guanidine hydrochloride (GuHCl) was monitored by means of fluorescence and circular dichroism spectroscopy Reversible dissociation of the dimeric enzyme into subunits was attained by the addition of 2 M GuHCl. The perturbation of the secondary structure under the denaturation condition resulted in the release of the cofactor FMN. Separation of the processes of refolding and reassociation of the monomeric species was achieved by the immobilization method. Dimeric PNP oxidase was immobilized by the covalent attachment to Affi-gel 15 without any significant lass of its catalytic activity. Matrix-bound monomeric species were obtained from the reversible refolding processes. The matrix bound-monomer was found to be catalytically active, possessing only a slightly decreased specific activity when compared to the refolded dimeric enzyme. In addition, limited chymotrypsin digestion of the oxidase yields two fragments of 12 and 161 kDa with a concomitant increase of catalytic activity The catalytically active fragment was isolated by ion exchange chromatography and analyzed for association of two subunits using the FPLC gel filtration analysis. The retention time indicated that the catalytic fragment of 16 kDa behaves as a compact monomer. Taken together, these results are consistent with the hypothesis that the native quaternary structure of PNP oxidase is not a prerequisite for catalytic function, but it could play a role in the regulation.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.40 no.4
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• pp.13-20
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• 2003

The model of the transistor saturation current of the BJT for integrated circuits based upon the semiconductor physics is proposed. The method for calculating the doping profile in the base region using process conditions is presented and the method for calculating the base Gummel number of lateral PNP BJT and vertical NPN BJT is proposed. The transistor saturation currents of NPN BJT using 20V and 30V process conditions obtained from the proposed method show an average relative error of 6.7% compared with the measured data and the transistor saturation currents of PNP BJT show an average relative error of 6.0% compared with the measured data.

• Preventive Nutrition and Food Science
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• v.20 no.2
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• pp.148-152
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• 2015

To develop functionally and nutritionally improved whole soybean curd (WSC), the effects of partial (0~4%) replacement with pine needle powder (PNP) on the quality characteristics of WSC were investigated. The moisture content and pH ranged from 76.96~77.80% (wet basis) and 6.69~6.74, respectively, with no considerable differences. Lightness significantly decreased with higher PNP content in the formulation (P<0.05), as indicated by visual observation that the color of WSC became darker. Redness and yellowness also significantly decreased (P<0.05). The texture profile analysis indicated that WSC containing PNP was softer and less cohesive than control WSC. 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical scavenging activities significantly increased (P<0.05) with higher substitution of PNP, and they were well correlated. Results from the consumer test revealed that WSC with 1% PLP received the most favorable acceptance scores for sensory attributes, including overall acceptability. The present study indicated that the characteristics of PNP may play a role in improving WSC quality in terms of physicochemical, sensorial, and antioxidant characteristics.

• Journal of Microbiology
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• v.39 no.2
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• pp.139-141
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• 2001

Medium-chain length polyhydrexyalkanoic acids (MCL-PHAs) degrading bacterium was isolated from the soil. The bacterium was identified as Janthinobacterium lividum by its biochemical properties, cell membrane fatty acids composition, and 16S rDNA sequence analysis. The bacterium showed a similarity of 0.911 with J. lividum according to the cell membrane fatty acids analysis and a similarity of 97% in the 16S rDNA requence analysis. Culture supernatant of the bacterium skewed the highest depolymerase activity toward polyhydroxynonanoic acid (PHN) that did not degrade the poly-$\beta$-hydroxybutyric acid (PHB). The esterase activity was also detected with p-nitrophenyl (PNP) esters of fatty acids such as PNP-dodecanoic PNP-dodecanoic acid, PNP-decanoic acid, and PNP-hexanoic acid.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1998.11a
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• pp.5-8
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• 1998

기존의 바이폴라 논리회로에서 신호변환시 베이스 영역의 소수 캐리어를 빨리 제거 하기 위해서, 베이스 부분의 매몰충을 줄여서 npn트랜지스터의 베이스와 에피충과 기판사이에 병합 pnp 트랜지스터를 생성한 트랜지스터와 게이트 당 전달 지연 시간을 측정하기 위한 링-발진기를 설계, 제작하였다. 게이트의 구조는 수직 npn 트랜지스터와 기판과 병합 pnp 트랜지스터이다. 소자 시뮬레이션의 자료를 얻기 위하여 수직 npn 트랜지스터와 병합 pnp 트랜지스터의 전류-전압 특성을 분석하여 특성 파라미터를 추출하였다. 결과로서 npn 트랜지스터의 에미터의 면적이 기존의 접합넓이에 비해서 상당히 적기 때문에 에미터에서 진성베이스로 유입되는 캐리어와 가장자리 부분으로 유입되는 캐리어가 상대적으로 많기 때문에 이 많은 양은 결국 베이스의 전류가 많이 형성되며, 또 콜렉터의 매몰층이 거의 반으로 줄었기 때문에 콜렉터 전류가 적게 형성되어 이득이 낮아진다. 병합 pnp 트랜지스터는 베이스폭이 크고 농도 분포에서 에미터의 농도와 베이스의 농도 차이가 적기 때문에 전류 이득이 낮아졌다. 게이트를 연결하여 링-발진기를 제작하여 측정한 AC특성의 출력은 정현파로 논리전압의 진폭은 200mV, 최소 전달 지연시간은 211nS이며, 게이트당 최소 전달지연 시간은 7.26nS의 개선된 속도 특성을 얻었다.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2000.11a
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• pp.136-139
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• 2000

Fenton-like oxidation of para-nitrophenol(PNP) was studied using hydrogen peroxide iii combination with steeler's dust substituted for ferrous iron in Fenton's reaction. Various factors critical in the degradation of PNP were studied, including hydrogen peroxide dosage, concentration of steeler's dust. and initial pH. Experimental results showed that 1,000mg/L PNP and its oxidation intermediate could be mostly decomposed within 30m1n by 10g/L steeler's dust, 0.25% hydrogen peroxide, and initila pH of 3.0. The reaction rate constant (k) of CODcr concentration were calculated with the addition of steeler's dust(0.0059 min$^{-1}$ (g/L)$^{-1}$ ) and hydrogen peroxide(0.2965 min$^{-1}$ (%)$^{-1}$ ), respectively.

• Journal of Microbiology
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• v.34 no.1
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• pp.82-89
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• 1996

Purine nucleoside phosphorylase (PNP) was purified in Micrococcus luteus (M. luteus) using streptomycin sulfate and amomonium sulfate fractionation, three times by a Sephadex G-100 gel filtration and a DEAE-Sephadex A-50 ion exchange chromatography. The enzyme was purified 72 folds with a 11% recovery and showed a single band in a nondenaturing gel electrophoresis. The M. W. of PNP turned out to be 1.35 * 10$^{5}$ delton in G-150 gel filtration chromatography. The stability of the enzyme was increased by treatment with both substrates, MgCI$_{2}$ or CaCI$_{2}$, but not significantly kcal/mol. M. luteus PNP catalyzed the phosphorolysis of inosine, deoxyinosine, guanosine and deoxyguanosine with the Km value of 1.5 * 10$^{-3}$ M, 3.0 * 10$^{-3}$ M, 5.0 * 10$^{-4}$ M, respectively. The enzyme was reacted with adenosine, 1-methylnosine and 1-methylguanosine as substrates, which were shown to be poor substrates for mammalian enzyme.

• Proceedings of the KIEE Conference
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• 2002.11a
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• pp.141-144
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• 2002

The method of the analysis of the base Gummel number of the PNP BJT(Bipolar Junction Transistor) for integrated circuits based upon the semiconductor physics is proposed and the method of calculating the doping profile of the base region using process conditions is presented. The transistor saturation current obtained from the proposed method of PNP BJT using 20V and 30V process shows an averaged relative error of 6.7% compared with the measured data.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.2
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• pp.296-301
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• 2015

The effects of pine needle powder (PNP) on the physicochemical characteristics of tofu were investigated, and consumer acceptance test was conducted. The pH, moisture content, and lightness and yellowness significantly decreased with increasing PNP concentration from 0 to 0.8% (P<0.05). Hardness increased significantly with increasing PNP concentration (P<0.05). Antimicrobial effects of PNP incorporated into tofu were also investigated. Antioxidant activities of control and produced tofu were compared based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and total polyphenol contents. DPPH radical scavenging activity and total polyphenol contents increased significantly (P<0.05) and were well-correlated. Tofu with 0.2% PNP is recommended (with respect to overall preference score) for taking advantage of the functional properties of PNP without sacrificing consumer acceptability.