• Asian-Australasian Journal of Animal Sciences
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• 제14권4호
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• pp.455-461
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• 2001

The effect of exogenous gonadotrophins on superovulation in rabbits was examined. One hundred and sixteen sexually mature California, Chinchilla and New Zealand White rabbits were randomly allocated to control (100 IU hCG), PMSG-treated (100 IU HCG following 150 IU PMSG) and FSH-treated groups (0.3 mg/head /12 h for 3 days followed by 100 IU hCG). All does were mated after hCG injection and were sacrificed or laparotomized within 1 to 4 days postcoitus for counting the number of ovulation points. The number of ovulations was higher in FSH-treated animals than in the control and PMSG-treated groups (37.2 vs. 10.4 and 14.5, p<0.05). Follicle haemorrhagicum was observed in many cases in the PMSG-treated group. No significant difference in ovulation number was observed between left and right ovaries regardless of gonadotropin treatment. In another experiment, 2-cell stage embryos were collected at 26 h postmating and blastomeres were separated by mechanical pipetting or gentle pressure with a fine glass needle. Aggregated or chimeric embryos were produced from two single blastomeres from two breeds, New Zealand White and Chinchlla, with different coat colors. All the embryos were cultured in Ham's F-10 medium supplemented with 1.5% BSA (bovine serum albumin fraction V) and 10% PRS (pregnant rabbit serum), and incubated in a humidified atmosphere with 5% $CO_2$ at $38^{\circ}C$. After development to morula or early blastocyst, the embryos were transferred into the oviducts of recipient does. Results showed that 7 out of 10 does (70%) receiving intact embryos (control) became pregnant and 41 kits were delivered. However, no pregnancy was obtained from the recipient of either denuded demi- or aggregated embryos. It is suggested that embryos without zona pellucida could not develop to term in rabbits.

• Asian-Australasian Journal of Animal Sciences
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• 제18권4호
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• pp.470-474
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• 2005

The efficiency of a short-term treatment with an intravaginal device containing progesterone (CIDR) combined with pregnant mares' serum gonadotrophin (PMSG) and prostaglandin analogue ($PGF_{2\alpha}$) was evaluated for the induction of estrus, initiation of cyclic activity, and fertility in postpartum suckled yak cows. Seventy-five postpartum suckled yak cows were assigned to three treatments: (1) insertion of a CIDR intravaginal progesterone (1.9 g) (day 0), an administration of $PGF_{2\alpha}$ (0.2 mg i.m.) on day 6 and PMSG (1,000 IU i.m.) at the time of CIDR withdrawal on day 7 (CPP group, n=28); (2) an administration of $PGF_{2\alpha}$ (0.2 mg i.m.) on day 6 and PMSG (1,000 IU i.m.) on day 7 (PP group, n=21); (3) untreated animals served as the control (CG group, n=26). Seven yak bulls were placed in pastures with the cows for natural mating. Estrus rate in the CPP group (28/28) was higher (p<0.01) than in the PP group (6/21) and in the CG group (0/26) within 96 h after the end of treatment. The first service conception rate in the CPP group (21/28) was higher (p<0.01) compared with in the PP group (2/9) as judged by serum $P_4$ concentration $\geq$2.35 ng/ml on day 21 after breeding. It is concluded that a short-term progesterone treatment combined with PMSG and prostaglandin increased the proportion of yak cows that exhibited behavioral estrus with more synchronized estrus response and satisfactory conception rate in postpartum suckled yak cows.

• Clinical and Experimental Reproductive Medicine
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• 제38권1호
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• pp.18-23
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• 2011

Objective: Peroxiredoxins (Prxs) play an important role in regulating cellular differentiation and proliferation in several types of mammalian cells. This report examined the expression of Prx isotype I in the rat ovary after hormone treatment. Methods: Immature rats were injected with 10 IU of pregnant mare's serum gonadotropin (PMSG) to induce the growth of multiple preovulatory follicles and 10 IU of human chorionic gonadotropin (hCG) to induce ovulation. Immature rats were also treated with diethylstilbestrol (DES), an estrogen analogue, to induce the growth of multiple immature follicles. Northern blot analysis was performed to detect gene expression. Cell-type specific localization of Prx I mRNA were detected by in situ hybridization analysis. Results: During follicle development, ovarian Prx I gene expression was detected in 3-day-old rats and had increased in 21-day-old rats. The levels of Prx I mRNA slightly declined one to two days following treatment with DES. A gradual increase in Prx I gene expression was observed in ovaries obtained from PMSG-treated immature rats. Furthermore, hCG treatment of PMSG-primed rats resulted in a gradual stimulation of Prx I mRNA levels by 24 hours (2.1-fold increase) following treatment, which remained high until 72 hours following treatment. In situ hybridization analysis revealed the expression of the Prx I gene in the granulosa cells of PMSG-primed ovaries and in the corpora lutea of ovaries stimulated with hCG for 72 hours. Conclusion: These results demonstrate the gonadotropin and granulosa cell-specific stimulation of Prx I gene expression, suggesting its role as a local regulator of follicle development.

• 전력전자학회논문지
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• 제15권4호
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• pp.296-308
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• 2010

본 논문은 영구자석형 동기발전기를 이용한 풍력발전 시스템의 3병렬 운전과 계통연계 기술을 제안한다. 영구자석형 풍력발전 시스템에서 back-to-back 컨버터는 발전기와 계통에 직접 연결되므로 시스템 정격에 준하는 전력 반도체 소자와 필터가 요구된다. 본 논문은 시스템의 전력변환부를 3병렬로 연결하고 병렬 운전에 의해 발생할 수 있는 순환 전류는 적절한 내부 인덕터 선정으로 해결한다. 낮은 스위칭 주파수로 운전되는 대용량 풍력발전 시스템의 THD 규정을 만족시키기 위해 최적 설계된 LCL필터를 사용하고 LCL필터에 의해 발생할 수 있는 공진 문제는 전력이론을 통한 능동 댐핑 기법을 통하여 추가적인 손실 없이 보상한다. 또한 계통 왜곡 및 불평형 시 발생할 수 있는 전력 품질의 문제는 추가적인 보상 알고리즘을 적용하여 향상시킨다. 시뮬레이션 및 실험을 통하여 병렬 운전 시스템과 알고리즘의 타당성을 검증한다.

• Journal of Electrical Engineering and Technology
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• 제10권1호
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• pp.243-250
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• 2015

This paper proposes a novel skewed-type iron slot wedge that can improve both the cogging torque and the output power of a permanent magnet synchronous generator (PMSG). Generally the open slot structure is adopted in a PMSG due to its convenient winding work, but the high cogging torque is undesired. Firstly, an iron slot wedge was utilized to reduce the cogging torque of an open slot type PMSG. However, the output power of the machine decreased rapidly with this method. Thus, a proposed skewed type iron slot wedge is presented to improve the output power as well as the cogging torque as compared to the open slot type. Shape optimization of the skewed-type iron slot wedge is performed to simultaneously maximize the output power and reduce the cogging torque. The Kriging model based on the Halton sequence method and a genetic algorithm are used to optimize the design.

• 전력전자학회논문지
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• 제24권6호
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• pp.419-427
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• 2019

Several multilevel converter topologies have been proposed and compared. The three-level (3L) neutral-point-clamped (NPC) topology is promising and widely accepted. However, this topology suffers from uneven loss distribution among switches due to its fixed switching strategy. The 3L active NPC (ANPC) topology, which exhibits improved loss distribution profile, was proposed to address this disadvantage. The 3L T-NPC topology, a hybrid configuration of 2L and 3L NPC topologies, was introduced to address not only the loss distribution problem but also the reduction in the number of switches. In the present research, the application of these three topologies in PMSG-based medium-voltage wind turbines was investigated. The power devices considered were 10 kV IGCTs. Performance was evaluated in terms of a power loss of 10 kV IGCT for each NPC topology, which is a crucial indicator of thermal behavior, reliability, cost, and lifetime of any converter. The comparison was performed using ABB make 10 kV IGCT 5SHY17L9000 and the simulation tool PLECS.

• Journal of Power Electronics
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• 제15권5호
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• pp.1380-1391
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• 2015

This paper provides a loss analysis and comparison of high power semiconductor devices in 5MW Permanent Magnet Synchronous Generator (PMSG) Medium Voltage (MV) Wind Turbine Systems (WTSs). High power semiconductor devices of the press-pack type IGCT, module type IGBT, press-pack type IGBT, and press-pack type IEGT of both 4.5kV and 6.5kV are considered in this paper. Benchmarking is performed based on the back-to-back type 3-level Neutral Point Clamped Voltage Source Converters (3L-NPC VSCs) supplied from a grid voltage of 4160V. The feasible number of semiconductor devices in parallel is designed through a loss analysis considering both the conduction and switching losses under the operating conditions of 5MW PMSG wind turbines, particularly for application in offshore wind farms. This paper investigates the loss analysis and thermal performance of 5MW 3L-NPC wind power inverters under the operating conditions of various power factors. The loss analysis and thermal analysis are confirmed through PLECS Blockset simulations with Matlab Simulink. The comparison results show that the press-pack type IGCT has the highest efficiency including the snubber loss factor.

• 한국발생생물학회지:발생과생식
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• 제1권2호
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• pp.165-172
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• 1997

the pupose of this study was to investigate the effects of gonadotropin and nitric oxide (NO) on the expression of mouse follicular bad and bax genes that are known induce apoptosis. Large and midium size follicles of immature mice were obtained at 0, 24, and 48 hours time intervals after Pregnant Mare's Serum gonadotropins(PMSG, 5 I.U.) injection. Preovulatory follicles collected at 24 hrs after PMSG injection were cultured with or without various chemicals such as gonadotropin, gonadotropin Releasing hormone(GnRH), testosterone, Sodium nitroprusside (SNP) for 24 hrs at $37^{\circ}C$. After 24 hrs culture, the culture media was used for nitrite assay and total RNA was extracted, subjected to RT-PCT for the analyses of bad and bax expression. We found that expression of bad and bax genes in follicles was markedly reduced before and after in vivo priming with hCG. When the preovulatory follicles were cultured for 24 hrs in culture media with PMSG and hCG, the expression of bad and bax genes was decreased. Moreover, SNP (NO generating agent) can significantly suppress the expression of bad and bax genes in follicles when apoptosis was induced by GnRH agonist and testosterone. At the same time, nitrite production of culture media was increased in GnRH agonist + SNP, testosterone + SNP and SNP treated groups than control group. These data demonstrated for the first time that peptide hormones and NO may play important roles in the regulation of mouse follicular differentiation and may prevent apoptosis via supressing the expression of bad and bax genes.

• 한국수정란이식학회지
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• 제7권2호
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• pp.89-96
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• 1992

This research was conducted to investigate the interrelationship among methods of injection of PMSG-hCG to the number of ovulated eggs, percentage of matured oocytes and in vitro fertilization using out-bred ICR mice. The results obtained are as follows, 1) The optimurn dose was 5 IU for both PMSG and hCG, while the number of ovulated eggs was 42$\pm$8, percentage of M II was 73% and in vitro fertilization rate was 81 %. 2) The optimum injection interval of PMSG-hCG was 48 hours, while the number of ovulated eggs was 48 $\pm$ 8, percentage of M II was 80% and in vitro fertilization rate was 81%. 3) The optimum time for collecting eggs was between 16 and 18 hours after hCG injection, while the numbers of ovulated eggs were 44$\pm$8, 42$\pm$7 and 43$\pm$7 in 14,16 and 18 hours after hCG injection respectively, and percentages of M II were 79 and 81 %, and in vitro fertilization rates were 81 and 80% in 16 and 18 hours after hCG injection, respectively. 4) The repeat of superovulation decreased with the number of ovulated eggs, percentage of M II and in vitro fertilization rate, than in control. But it was recovered by increasing the repeat interval.

• 한국수정란이식학회지
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• 제13권2호
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• pp.127-137
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• 1998

This experiment was carried out to investigate the factors affecting superovulation in rabbits and to determine the effect of pFSH and PMSG on ovarian superovulatory responses and embryo production, and the effect of superovulation treatment with a single injection of pFSH dissolved in polyvinylpyrrolidone on the ovarian responses and the embryo quality. The results obtained were suonmerized as follows: Superovulatory response resulted in significantly (P<0.05) higher ovulation rates and more embryos in spring or autumn, compared with summer or winter. Repeated superovulatory treatments with PMSG leaded to a significantly(P<0.05) decreased number of total follicles and recovered ova. Superovulation with pFSH resulted in the higher number of ovulated follicles and recovered ova than with PMSG. A single subcutaneous injection of pFSH dissolved in 25% PVP resulted in the more ovulation points(33.2) and recovered embryos(30.2), which were comparable to the multiple injections of pFSH(44.8 vs 37.7).These results indicated that the treatment with a single injection of FSH dissolved in PVP was an efficient and simple alternative method to the conventional multiple FSH injections for superovulation in rabbits.