• Biomolecules & Therapeutics
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• v.1 no.2
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• pp.131-136
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• 1993

In the present study, the effect of $HgCl_2$on the function of human peripheral polymorphonuclear leukocytes(PMNs) was examined. PMNs were isolated from human peripheral blood with density centrifugation in Ficoll-Paque. The cells were then incubated with $0.5{\sim}5{\mu}M\;HgCl_2$and glass adherence, chemotactic activity and erythrocyte-antibody rosette forming activity were measured. $HgCl_2$ decreased the function of PMNs in all three aspects tested. $HgCl_2$significantly diminished glass adherence(40.5 {\mu}M: 92{\pm}12%$ (percentage of control, $mean{\pm}$ S.D.); 41 {\mu}M: 46{\pm}11%,$ P<0.01; $3{\mu}M: 35{\pm}7%,$P<0.01;$5{\mu}M:49{\pm}10%,$ P<0.01). Similarly, significant differences were observed in chemotactic activity after $HgCl_2$treatment compared with control (control: $0.95{\pm}0.14mm; 0.5 {\mu}M: 0.91{\pm}0.11 mm; 1 {\mu}M: 0.77{\pm}0.16mm, P<0.05; 3{\mu}M: 0.61{\pm} 0.06mm, P<0.01; 5{\mu}M: 0.15{\pm}0.03 mm, P<0.01).$ Also, 4HgCl_2$decreased the percentage of rosette-forming PMNs, indicating diminished phagocytic activity of PMNs upon $HgCl_2$ exposure compared with control (control: $58{\pm}4%; 1{\mu}M: 53{\pm}4%, p<0.05; 3{\mu}M: 49{\pm}3%, P<0.01; 5{\mu}M: 46{\pm}3%, P<0.01).$ Cell viability was not antered after $HgCl_2$treatment (483{\pm}5%$ viability in control PMNs versus $81{\pm}8%$ viability in $5{\mu}M$ Hg-treated PMNs), suggesting that the impaired PMN function after $HgCl_2$treatment was not due to nonspecific cytotoxicity induced by $HgCl_2$. $HgCl_2$-induced decrease in the function of PMNs may have some implications in depressed host susceptibilityupon bacterial challenge after mercury exposure.

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.229-234
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• 2002

This study was carried out to investigate the general characteristics such as concentration sperm motility and abnormality of sperm on the whole epididymal semen(EWS), RSP-S(removed seminal plasma by saline) and RSP-T(removed seminal plasma by tris-buffer) semen and survival rates after freezing on motility of whole and RSP-S and RSP-T semen and extender containing 2~8% glycerol, and ability of frozen-thawed sperm to penetrate homologous oocytes. 1. The concentration, motility and abnormality of epididymal WES, RSP-S and RSP-T sperm were 4.25 $\pm$ 0.25($\times$10$^{6}$ Cells/$m\ell$), 3.85$\pm$0.20($\times$10$^{6}$ Cells/$m\ell$), 4.05 $\pm$ 0.28($\times$10$^{6}$ Cells/$m\ell$), 50.55 $\pm$ 2.75%, 67.25 $\pm$ 2.55%, 78.75 $\pm$ 3.55 and 9.45 $\pm$ 2.25%, 37.75 $\pm$ 2.10%, 24.25 $\pm$ 1.55%, respectively. 2. The survival rates of slow and rapid frozen epididymal RSP-S and RSP-T sperm were 35.00 $\pm$ 2.35%, 45.50 $\pm$ 2.15％ and 16.50 $\pm$ 3.55%, 22.55 $\pm$ 3.95%, respectively. The survival rate of epididymal WES and RSP-T sperm after freezing following dilution with tris-buffer containing 2~8% glycerol were 9.25 $\pm$ 1.55%~17.50 $\pm$ 2.50%. 3. The percentage of capacitated and acrosome-reacted sperm prier to culture for fresh and frozen -thawed epididymal RSP-T semen were 45.25 $\pm$ 5.75%, 7.06 $\pm$ 0.25%, 48.20 $\pm$ 6.80% and 13.00 $\pm$ 2.35%, 3.55 $\pm$ 0.85%, 15.50 $\pm$ 1.90%, respectively. The penetration rate the number of sperm per penetrated for fresh and frozen-thawed epididymal RSP-T sperm were 39.25 $\pm$ 4.72%, 34.24 $\pm$ 3.93% and 1.30 $\pm$ 0.33, 1.10 $\pm$ 0.50., respectively.

• Journal of Environmental Health Sciences
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• v.31 no.4 s.85
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• pp.301-308
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• 2005

The purpose of this study was to characterize mass concentration of $PM_{10}\;to,\;PM_{2.5}$ and metallic composition using Minivol Portable Sampler from May 2003 to May 2004 in metropolitan city, Seoul. Annual average concentration of $PM_{10}$ were $57.67({\pm}28.20)\;{\mu}g/m^3\;and\;PM_{2.5},\;were\;42.06({\pm}20.23)\;{\mu}g/m^3$. The concentration was the highest in winter because persistent thermal inversion and in spring, particulate matter concentration was high because of yellow-sand events. The average $PM_{2.5}/PM_{10}$ ratio was 0.73. This indicated $PM_{2.5}$ fraction played a significant role in air pollution. The atmospheric metallic elements in the $PM_{10},PM_{2.5}$ came different emission sources such as soil, traffic, industry and resuspended particles. The results showed that average $PM_{10}$ composition order as Fe>Zn>Pb>Cu>Mn. The metallic elements Zn and Fe were the largest composition in the $PM_{2.5}$

• 월간산업보건
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• s.242
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• pp.55-59
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• 2008

• Proceedings of the Korea Air Pollution Research Association Conference
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• 1999.04a
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• pp.21-23
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• 1999

• Proceedings of the Korea Air Pollution Research Association Conference
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• 1999.04a
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• pp.270-271
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• 1999

• Journal of Animal Science and Technology
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• v.44 no.2
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• pp.191-200
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• 2002

This study was conducted to estimate the effects of environmental factors on certain reproductive traits in Korean cattle on the basis of data from Korean Cattle Improvement Areas which were surveyed from 1986 to 1995. Results obtained were summarized as follows ; 1. Least square means of the reproductive traits estimated were 466.11${\pm}$5.72 days for age at 1st service, 480.10${\pm}$6.15 days for age at 1st conception, 742.79${\pm}$7.48 days for age at 1st calving, 64.97${\pm}$1.42 days for days at 1st service postpartum, 72.06${\pm}$1.73 days for days open, 355.93${\pm}$1.92days for calving interval, 285.38${\pm}$0.42 days for gestation length and 1.21${\pm}$0.02 days for number of services for conception, respectively. 2. The ranges of the least squares means for the districts were 447.62${\pm}$5.94-490.72${\pm}$5.94 days for age at 1st service, 464.27${\pm}$6.38-505.20${\pm}$6.39 days for age at 1st conception, 721.91${\pm}$7.85-772.75${\pm}$7.80 days for age at 1st calving, 62.27${\pm}$1.55-68.97${\pm}$1.74days for days at 1st service postpartum, 69.74${\pm}$1.97-74.36${\pm}$2.27days for days open, 352.71${\pm}$2.18-359.64${\pm}$2.09 days for calving interval, 284.52${\pm}$0.42-286.04${\pm}$0.47days for gestation length and 1.11${\pm}$0.02-1.30${\pm}$0.02 days for number of services for conception, respectively. 3. Age at 1st service was latest in the cows with Basic Register and tended to be earlier for the more advanced registers. The number of services per conception tended to increase for the more advanced registers. 4. Days at 1st service postpartum was longest in the 2nd parity and tended to be shorter with the advancement of parity. The number of services per conception tended to decrease with the advancement of parity. 5. As the calving year advances from 1987 to 1995, days at 1st service postpartum, days open and calving interval tended to be longer, and number of service per conception tended to increase. 6. Days at 1st service postpartum, days open and calving interval were shorter in the cows that calved in spring and summer than in the cows that calved in fall and winter. The number of services per conception was larger in the cows that cocalved in fall and winter than the cows that calved in spring and summer.

• Journal of Chest Surgery
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• v.28 no.5
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• pp.471-474
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• 1995

A total of 20 patients underwent bullectomy in the spontaneous pneumothorax between October 1993 and August 1994. The patients were divided into two groups: Control group; the patients who received with mid-axillary approach[n=10 , Experimental group; the patients who received with video-assisted thoracic surgery [n = 10 . The results were as follows; 1. The total sex distribution was male predominence [M :F=6:1 . Mean age of control group was 31.6$\pm$ 10.1 age and experimental group was 24.3$\pm$ 5.5 age. 2. The operative times were 117.0 $\pm$ 32.6min in control group and 102.5$\pm$ 38.4min in experimental group [not significant . 3. The indwelling period of postoperative chest tube and hospital stay were 4.5$\pm$ 2.6 days and 8.3 $\pm$ 1.8 days in control group, $1.5\pm$ 0.5 days and 3.1 $\pm$ 0.3 days in experimental group[p=0.0018, < 0.0001 . 4. In control group, injection times of pain-killer were 1.7$\pm$ 0.7 times/day at operative day and 0.4$\pm$ 0.6times/day at postoperative 1 day. In experimental group, there were 0.3$\pm$ 0.7times/day at operative day and 0.1 $\pm$ 0.3times/day at postoperative 1 day[p=O.O002 at operative day, not significant at postoperative 1 day .

• Journal of Environmental Health Sciences
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• v.26 no.2
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• pp.22-29
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• 2000

This study was performed to investigate the concentrations of TSP, PM10 and heavy metals(Pb, Cd,Hg, Ni, Zn) of underground parking lots in Taegu city. The samples were collected from 3 department stores and 1 central park in the winter and the summer, 1997. The samples of 3 department stores were divided into sale period and non-sale period in the winter and the summer. The concentrations of TSP and PM10 were 109.6±1.5㎍/㎥ and 93.3±1.5㎍/㎥. In TSP, the zinc was the highest level, 287.16±1.5ng/㎥ and the cadmium was the lowest, 2.4±2.1ng/㎥ and in PM10, the zinc was the highest level, 193.6±1.5ng/㎥ and the cadmium was the lowest, 0.6±3.9ng/㎥. The correlation coefficient between the concentration of TSP and PM10 was 0.982(p<0.05). The correlation coefficients of corresponding heavy metal in TSP and PM10 were 0.863 for lead, 0.617 for mercury, 0.890 for nickel and 0.850 for zinc(p<0.05). The concentrations of TSP, PM10 and cadmium of PM10 in the winter were higher than those of the summer. However, the concentration of Hg of PM10 in the summer was higher than that of the winter. The concentrations of TSP and PM10 in sale period was higher than those of non-sale period and the concentrations of heavy metal in TSP and PM10 were not significantly different between sale and non-sale period. The proportions of PM10 to TSP were above 60% in dust, lead, nickel, and zinc and less than 40% in cadmium and mercury. The concentrations of TSP, PM10 and heavy metal in the underground parking lots were comparatively lower than those of general atmosphere. However, this kind of research to PM10 in the underground parking lots must be continued because it is very important particulate matter that affects human beng's health.

• Journal of Chest Surgery
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• v.33 no.7
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• pp.552-559
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• 2000

Background; The aim of this study is to evaluate our clinical experience with the Carbomedics heart valve prosthesis. Material and Method; Between Aug. 1988 and Dec. 1998, 294 Carbomidics valves were implanted in 235 patients(mitral; 143, mitral and aortic; 59, aortic; 33) The mean age at operation is 40.0$\pm$12.3 years(range 7 to 68 years); 63.8% (150patients) were woman. Follow up was 97.4% complete and mean follow up time was 5.7years with a total of 1209.2 patient-years. Result; The hospital mortality was 8.9%(mitral; was 95.2$\pm$1.6%(mitral ; 94.9$\pm$2.1%, mitral and aortic 95.0$\pm$3.7%, aortic 96.2$\pm$3.8%). Actual freedom rates from complications(linearized rates in parentheses) were fllowings; thromboembolism 96.2$\pm$1.5%(0.59%pt-yr), valve thrombosis 96.7$\pm$1.4%(0.5%/pt/yr), anticoagulant related hemorrhage 98.3$\pm$1.0%(0.25%/pt-yr), perivalvular leak 99.0$\pm$1.4%(0.5%/pt-yr), endocarditis 98.7$\pm$1.0%(0.25%/pt-yr), perivalvular leak 99.0$\pm$0.7%(0.17%pt-yr), endocarditis 98.7$\pm$1.0%(0.17%$\pm$pt-yr) and overall valve-related complications 88.9$\pm$2.5%(1.68%/pt-yr). Conclusion; The clinical performance of the Carbomedics valve was quite satisfactory with a low incidence of valve related mortality and morbidity.