• Journal of KIISE
• /
• v.42 no.11
• /
• pp.1372-1379
• /
• 2015

This paper presents an analysis of feature extraction methods used for distinguishing the speech of patients with cleft palates and people with normal palates. This research is a basic study on the development of a software system for automatic recognition and restoration of speech disorders, in pursuit of improving the welfare of speech disabled persons. Monosyllable voice data for experiments were collected for three groups: normal speech, cleft palate speech, and simulated clef palate speech. The data consists of 14 basic Korean consonants, 5 complex consonants, and 7 vowels. Feature extractions are performed using three well-known methods: LPC, MFCC, and PLP. The pattern recognition process is executed using the acoustic model GMM. From our experiments, we concluded that the MFCC method is generally the most effective way to identify speech distortions. These results may contribute to the automatic detection and correction of the distorted speech of cleft palate patients, along with the development of an identification tool for levels of speech distortion.

• Molecules and Cells
• /
• v.41 no.12
• /
• pp.1033-1044
• /
• 2018

As sessile organisms, plants have evolved to adjust their growth and development to environmental changes. It has been well documented that the crosstalk between different plant hormones plays important roles in the coordination of growth and development of the plant. Here, we describe a novel recessive mutant, mildly insensitive to ethylene (mine), which displayed insensitivity to the ethylene precursor, ACC (1-aminocyclopropane-1-carboxylic acid), in the root under the dark-grown conditions. By contrast, mine roots exhibited a normal growth response to exogenous IAA (indole-3-acetic acid). Thus, it appears that the growth responses of mine to ACC and IAA resemble those of weak ethylene insensitive (wei) mutants. To understand the molecular events underlying the crosstalk between ethylene and auxin in the root, we identified the MINE locus and found that the MINE gene encodes the pyridoxine 5′-phosphate (PNP)/pyridoxamine 5′-phosphate (PMP) oxidase, PDX3. Our results revealed that MINE/PDX3 likely plays a role in the conversion of the auxin precursor tryptophan to indole-3-pyruvic acid in the auxin biosynthesis pathway, in which TAA1 (TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1) and its related genes (TRYPTOPHAN AMINOTRANSFERASE RELATED 1 and 2; TAR1 and TAR2) are involved. Considering that TAA1 and TARs belong to a subgroup of PLP (pyridoxal-5′-phosphate)-dependent enzymes, we propose that PLP produced by MINE/PDX3 acts as a cofactor in TAA1/TAR-dependent auxin biosynthesis induced by ethylene, which in turn influences the crosstalk between ethylene and auxin in the Arabidopsis root.

• Culinary science and hospitality research
• /
• v.17 no.3
• /
• pp.172-180
• /
• 2011

This study explores the quality characteristics of white pan bread added with perilla leaf powder, and the results are as follows. The WRC(water retention capacity) of PLP (perilla leaf powder) added to the bread dough increased as the addition increased, and the initiation temperature during the rapid visco analysis (RVA) increased with more additions, showing significant differences. The highest and the lowest viscosity of the sample decreased as the powder added increased. The brightness(L value) of the bread without the powder was the highest, and the redness (a value) and the yellowness (b value) showed significant differences among the samples. Hardness increased showing significant differences among the samples as the powder added increased Chewiness was the highest but preference value was the lowest in the sample with 7% of the powder. The lowest score was shown in the color of the sample with the most PLP added. Flavor was the lowest of 4.93 in the comparison sample without the leaf powder added while bitter taste scored the highest of 5.21 in the sample of 3% powder added. It was also the highest in overall acceptance while the sample with the most powder showed the lowest of 4.03. From these results, it can be said that the preference of bread with PLP seems to decrease when more than 5% of PLP is added.

• Journal of Nutrition and Health
• /
• v.36 no.4
• /
• pp.389-396
• /
• 2003

Maternal nutritional status has been shown to influence pregnancy outcomes. And the elevated maternal plasma homocysteine concentrations have been associated with adverse pregnancy outcomes. We investigated the effects of maternal serum levels of B vitamins and homocysteine, and the C677T MTHFR (5, 10-methylenetetrahydrofolate reductase) polymorphism on pregnancy outcomes. In 177 pregnant women of 24-28 wks of gestation, the MTHFR gene mutation, serum B vitamins and homocysteine concentrations were measured, and their pregnancy outcomes were investigated from medical records. The birth length, and 1- and 5-min Apgar scores of neonates in the T/T mothers were 45.4 $\pm$ 9.3 cm, 7.6 $\pm$ 3.2 and 8.5 $\pm$ 3.8, respectively, which were significantly lower than those in the C/T (48.6 $\pm$ 3.3 cm, 9.0 $\pm$ 0.2, 10.0 $\pm$ 0.2) or the C/C mothers (49.4 $\pm$ 1.9 cm, 9.0 $\pm$ 0.2, 10.0 $\pm$ 0.0). The birth weight, birth length and the gestational age of neonates at delivery from hyperhomocysteinemic mothers whose homocysteine levels higher than 15 $\mu$ mol were 2.5 $\pm$ 1.3 kg, 43.9 $\pm$ 9.0 cm, 35.4 $\pm$ 6.3 wk, respectively, which were significant lower than those from normohomocysteinemic mothers (3.1 $\pm$ 0.6 kg, 48.8 $\pm$ 3.6 cm, 38.5 $\pm$ 2.5 wk). The birth weight and birth length of neonates in mothers whose PLP levels were below the median were significantly lower than those from mothers with the PLP levels above the median. The 1- and 5-min Apgar scores of neonates were lower in mothers with the T/T MTHFR genotype than those with the C/T or C/C only when the serum PLP levels were below the median. The 1-, 5 min Apgar scores and birth length of neonates were lower in mothers with the T/T MTHFR genotype than those with the C/T or C/C only when the serum FMN levels were below the median. In conclusion, maternal B vitamin status, homocysteine and the C677T MTHFR genotype seem to have played an important role on pregnancy outcomes.

• BMB Reports
• /
• v.41 no.5
• /
• pp.408-413
• /
• 2008

Pyridoxal-5'-phosphate phosphatase (PLPP) catalyzes the dephosphorylation of pyridoxal-5'-phosphate (PLP). A human brain PLPP gene was fused with a PEP-1 peptide and produced a genetic in-frame PEP-1-PLPP fusion protein. The purified PEP-1-PLPP fusion protein was efficiently transduced into PC12 cells in a time- and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced PEP-1-PLPP fusion protein was stable for 36 h. The concentration of PLP was markedly decreased by the addition of exogenous PEP-1-PLPP to media pretreated with the vitamin $B_6$ precursors; pyridoxine, pyridoxal kinase and pyridoxine-5'-phosphate oxidase into cells. The results suggest that the transduction of the PEP-1-PLPP fusion protein can be one mode of PLP level regulation, and to replenish this enzyme in the various neurological disorders related to vitamin $B_6$.

• Korean Journal of Food Science and Technology
• /
• v.46 no.4
• /
• pp.438-445
• /
• 2014

We examined biogenic amine (BA) production as well as the diversity of bacterial flora in 11 types of commercial makgeolli stored at 4 and $20^{\circ}C$. Moreover, we studied the BA-producing activity of three L. plantarum strains isolated from makgeolli. At $20^{\circ}C$, the BA content was highly increased and the denatured DNA bands were more variable in non-sterilized makgeolli compared to sterilized makgeolli. The major BAs produced in commercial makgeolli were histamine and putrescine. Histamine, tyramine, putrescine, and cadaverine were produced in excess by inoculation of the three L. plantarum isolates to makgeolli stored at $20^{\circ}C$ for 21 days. These results suggest that some L. plantarum strains in makgeolli can produce different types of BAs, depending on the extent of degradation of makgeolli.

• Journal of the Korean Institute of Gas
• /
• v.27 no.4
• /
• pp.19-26
• /
• 2023

Earthquakes are one of the most important disasters affecting underground structures. Urban gas underground pipes may cause safety problems of structures in the event of an earthquake. Since Korea began digital observation, the number of earthquakes has been steadily increasing. The seismic design standard for urban gas pipes was established in 2008, but it is difficult to estimate the impact of pipes in the event of an earthquake based on the installation of pipes. In this study, structural analysis was performed on PE (polyethylene pipe) pipes and PLP (polyethylene coated steel pipe) pipes, which are mainly used as buried pipes in Korea, according to environmental and pipe variables in the event of an earthquake. This study sought to find the variables of the most vulnerable buried pipe by modeling pipes through Computer Aided Engineering (CAE) and generating displacement on the ground. Through this study, it was confirmed that the larger the elastic modulus of the soil, the deeper the buried depth, the smaller the tube diameter, and the higher the pressure, the more PLP pipes are affected by earthquakes than PE. Based on these results, the vulnerable points of buried urban gas pipes are inferred and used for special inspections of buried pipes in the event of an earthquake.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.11
• /
• pp.5333-5338
• /
• 2012

Vitamin B6 functions as a coenzyme in >140 enzymatic reactions involved in the metabolism of amino acids, carbohydrates, neurotransmitters, and lipids. It comprises a group of three related 3-hydroxy-2-methyl-pyrimidine derivatives: pyridoxine (PN), pyridoxal (PL), pyridoxamine (PM) and their phosphorylated derivatives [pyridoxal 5'-phosphate (PLP) and pyridoxamine 5'-phosphate (PMP)], In the folate metabolism pathway, PLP is a cofactor for the mitochondrial and cytoplasmic isozymes of serine hydroxymethyltransferase (SHMT2 and SHMT1), the P-protein of the glycine cleavage system, cystathionine ${\beta}$-synthase (CBS) and ${\gamma}$-cystathionase, and betaine hydroxymethyltransferase (BHMT), all of which contribute to homocysteine metabolism either through folate-mediated one-carbon metabolism or the transsulfuration pathway. Folate cofactors carry and chemically activate single carbons for the synthesis of purines, thymidylate and methionine. So the evidence indicates that vitamin B6 plays an important role in maintenance of the genome, epigenetic stability and homocysteine metabolism. This article focuses on studies of strand breaks, micronuclei, or chromosomal aberrations regarding protective effects of vitamin B6, and probes whether it is folate-mediated one-carbon metabolism or the transsulfuration pathway for vitamin B6 which plays critical roles in prevention of cancer and cardiovascular disease.

• The Journal of the Acoustical Society of Korea
• /
• v.33 no.4
• /
• pp.268-272
• /
• 2014

This paper presents a method for speech recognition using multi-section vector-quantization (MSVQ) and time-delay recurrent neural network (TDTNN). The MSVQ generates the codebook with normalized uniform sections of voice signal, and the TDRNN performs the speech recognition using the MSVQ codebook. The TDRNN is a time-delay recurrent neural network classifier with two different representations of dynamic context: the time-delayed input nodes represent local dynamic context, while the recursive nodes are able to represent long-term dynamic context of voice signal. The cepstral PLP coefficients were used as speech features. In the speech recognition experiments, the MSVQ/TDRNN speech recognizer shows 97.9 % word recognition rate for speaker independent recognition.

• Journal of Integrative Natural Science
• /
• v.11 no.3
• /
• pp.131-138
• /
• 2018

In this paper, we have implemented speeches that utilized the emotion information of the user's speech and image matching and recommendation system. To classify the user's emotional information of speech, the emotional information of speech about the user's speech is extracted and classified using the PLP algorithm. After classification, an emotional DB of speech is constructed. Moreover, emotional color and emotional vocabulary through factor analysis are matched to one space in order to classify emotional information of image. And a standardized image recommendation system based on the matching of each keyword with the BM-GA algorithm for the data of the emotional information of speech and emotional information of image according to the more appropriate emotional information of speech of the user. As a result of the performance evaluation, recognition rate of standardized vocabulary in four stages according to speech was 80.48% on average and system user satisfaction was 82.4%. Therefore, it is expected that the classification of images according to the user's speech information will be helpful for the study of emotional exchange between the user and the computer.