• 한국양식학회지
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• 제19권3호
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• pp.157-165
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• 2006

우리나라 주요 담수 어종인 미꾸라지를 ecotoxicogenomic 연구 모델 어류로 개발하기 위한 연구의 일환으로 본 어종이 고온 스트레스 자극에 노출되었을때 야기되는 산화성 스트레스를 검출하고자 항산화 효소(antioxidant enzyme; AOE) 유전자의 발현 양상을 분석하였다. 주요 항산화 효소인 superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) 및 glutathione peroxidases (GPXs)의 transcript들을 특이적으로 정량화할 수 있는 semi-quantitative RT-PCR, real-time PCR 또는 northern blot분석을 통해 $23^{\circ}C$에서 $32^{\circ}C$까지 설정된 실험어의 간 조직내 AOE유전자들의 mRNA level을 분석하였다. 고온에 노출되었을 때 본 어종의 AOE들은 일반적으로 증가된 유전자 발현 양상을 나타내었고, 특히 SOD (2배)와 plasma GPX (3배) 유전자가 가장 유의적인 mRNA 증가를 나타내었다. GST의 경우 상대적으로 적은 증가량을 나타내었고 CAT의 경우 고온자극에 반응하지 않았다. 본 어종은 $29^{\circ}C$ 이상에서 AOE 유전자의 발현 증가를 나타내었고 $32^{\circ}C$에 노출되었을 때 1일째부터 SOD와 plasma GPX mRNA의 증가가 관찰되었다.

• 한국해양공학회지
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• 제22권6호
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• pp.41-45
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• 2008

Effectsof carbon content on the weldability of B-containing 620 grade high Cr ferritic cast steels were investigated. Cast steel with lower carbon content of 0.07% showed lower HAZ hardness because of the formation of lower carbon martensite in HAZ. It also showed less solidification cracking susceptibility in weld metal because of the formation of delta ferrite. However, hot ductility showed no difference between cast steels with lower and higher carbon contents. Cast steel with lower carbon content showed greater HAZ softening after PWHT in the region heated between AC1 and AC3 because of its higher base metal hardness.

• 한국기계가공학회지
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• 제19권3호
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• pp.86-91
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• 2020

An important factor of rough road modeling is analyzing the shear behavior properties of the rough road. These properties influence the drawbar pull of the tool when interacting with the soil used in agriculture. Furthermore, shear behavior properties are important because sinkage and shear stress are generated when wheels drive on rough roads. In this study, we performed a direct shear test to investigate the shear behavior properties of soils and compare with the direct shear simulation; shear force derived by the coupled analysis of discrete element method; and multi-body dynamics. Soil contact parameters were measured in a wheel and soil contact simulation followed by comparison of the simulated and experimentally measured shear force.

• Fisheries and Aquatic Sciences
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• 제18권3호
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• pp.241-247
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• 2015

In an effort to discover alternative phytotherapeutic antimicrobial agents to combat Streptococcus parauberis, a fish pathogenic bacterium, we evaluated the antibacterial activity of seaweed extracts in vitro. A methanolic extract of Ecklonia cava exhibited strong antibacterial activity against S. parauberis isolated from olive flounder Paralichthys olivaceus. Furthermore, the n-hexane soluble (Hexane) fraction of the E. cava methanolic extract exhibited the greatest antibacterial effect on S. parauberis strains with a minimum inhibitory concentration (MIC) ranging from 256 to $1,024{\mu}g/mL$. In addition, the MIC values of oxytetracycline against antibiotic-resistant S. parauberis were markedly reduced up to 64-fold in combination with the Hexane fraction, suggesting that the antibacterial activity of the antibiotic was restored when combined with the Hexane fraction. The interaction between both antibiotics and the Hexane fraction was assessed by the fractional inhibitory concentration (FIC) index. The Hexane fraction and oxytetracycline combination against antibiotic-resistant S. parauberis strains resulted in a median ${\sum}FIC$ range of 0.502 to 0.516. Thus, the synergistic ranges of median ${\sum}FIC$ < 1 were observed for all combinations of the Hexane fraction and oxytetracycline against S. parauberis. To the best of our knowledge, this is the first report indicating the efficacy of an E. cava extract against fish pathogenic bacterium S. parauberis.

• Fisheries and Aquatic Sciences
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• 제21권10호
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• pp.33.1-33.11
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• 2018

As a process for commercial application, production of reducing sugar, antioxidant, and DNA protective compounds from shrimp-shell powder was investigated in a fed-batch biodegradation using Bacillus cereus EW5. The fed-batch biodegradation was operated in a 5-L bioreactor for 96 h according to three times pulse-feeding strategy. On the basis of the equal working volume (3 L), the fed-batch biodegradation showed a better production of the target compounds than the batch biodegradation, with higher cell density and shortened biodegradation period. The maximum values of the target compounds were 0.297 mg/mL of reducing sugar, 92.35% DPPH radical scavenging activity, 98.16% ABTS radical scavenging activity, and 1.55 reducing power at $A_{700}$, which were approximately 12.1, 3.4, 5.2, and 8.4% enhanced, respectively, compared with those obtained from the batch biodegradation. The fed-batch culture supernatant also showed the enhanced DNA damage inhibition activity than the batch culture supernatant. As a result, the fed-batch biodegradation accompanied by high cell density could produce more useful compounds, enabling an increase in the reutilization value of shrimp-shell waste.

• Journal of Welding and Joining
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• 제26권6호
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• pp.54-58
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• 2008

Laser welding with AZ61 filler wire was carried out to improve formability though reduction of porosity and formation of under fill bead. Optimum welding condition and mechanical properties of butt joint for $400{\times}500{\times}1.3mm$ magnesium sheets were studied. Optimal welding conditions of laser power, welding speed, and defocusing length are 1000W, 3m/min, and 2mm, respectively. Results of tensile test indicated that both tensile strength and elongation of specimens welded with filler wire were improved at room temperature because of reduction of porosity and under-filled bead formation in addition to the precipitation hardening and microstructure refinement by Al-Mn and Mg-Al-Zn precipitates. At elevated temperature of $200{\sim}350^{\circ}C$, fracture location of tensile specimen was shifted from weld metal to base metal, indicating less softening of weld metal than base metal.

• 마이크로전자및패키징학회지
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• 제27권4호
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• pp.83-89
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• 2020

지난 수십년간 인류에게 핵심적인 에너지 자원이었던 화석연료가 갈수록 고갈되고 있고, 산업발전에 따른 오염이 심해지고 있는 환경을 보호하기 위한 노력의 일환으로, 친환경 이차전지, 수소발생 에너지 장치, 에너지 저장 시스템 등과 관련한 새로운 에너지 기술들이 개발되고 있다. 그 중에서도 리튬이온 배터리 (Lithium ion battery, LIB)는 높은 에너지 밀도와 긴 수명으로 인해, 대용량 배터리로 응용하기에 적합하고 산업적 응용이 가능한 차세대 에너지 장치로 여겨진다. 하지만, 친환경 전기 자동차, 드론 등 증가하는 배터리 시장을 고려할 때, 수명이 다한 이유로 어느 순간부터 많은 양의 배터리 폐기물이 쏟아져 나올 것으로 예상된다. 이를 대비하기 위해, 폐전지에서 리튬 및 각종 유가금속을 회수하는 공정개발이 요구되는 동시에, 이를 재활용할 수 있는 방안이 사회적으로 요구된다. 본 연구에서는, 폐전지의 재활용 전략소재 중 하나인, 리튬이온 배터리의 대표적 양극 소재 Li2CO3의 나노스케일 패턴 제조 방법을 소개하고자 한다. 우선, Li2CO3 분말을 진공 내 가압하여 성형하고, 고온 소결을 통하여 매우 순수한 Li2CO3 박막 증착용 3인치 스퍼터 타겟을 성공적으로 제작하였다. 해당 타겟을 스퍼터 장비에 장착하여, 나노 패턴전사 프린팅 공정을 이용하여 250 nm 선 폭을 갖는, 매우 잘 정렬된 Li2CO3 라인 패턴을 SiO2/Si 기판 위에 성공적으로 형성할 수 있었다. 뿐만 아니라, 패턴전사 프린팅 공정을 기반으로, 금속, 유리, 유연 고분자 기판, 그리고 굴곡진 고글의 표면에까지 Li2CO3 라인 패턴을 성공적으로 형성하였다. 해당 결과물은 향후, 배터리 소자에 사용되는 다양한 기능성 소재의 박막화에 응용될 것으로 기대되고, 특히 다양한 기판 위에서의 리튬이온 배터리 소자의 성능 향상에 도움이 될 것으로 기대된다.

• 한국항해항만학회지
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• 제27권3호
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• pp.283-288
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• 2003

Recently, the growth of applications and services over high-speed Internet increases, ATM networks as wide area back-bone has been a major solution. As the conventional TCP/IP suite is still the standard protocol used to support upper application on current. Internet, the issues regarding whether TCP/IP will operate efficiently on top of an ATM infrastructure and how to control its QoS still remain for studies. TCP uses a window-based protocol for flow control in the transport layer. When TCP uses the UBR service in ATM layer, the control method is only buffer management. If a cell is discarded in ATM layer, one whole packet of TCP will be lost; this fact occur the most TCP performance degradation. Several dropping strategies, such as Tail Drop, EPD, PPD, SPD, FBA, have been proposed to improve the TCP performance over ATM. In this paper, to improve the TCP performance, we propose a packet dropping scheme that is based on comparison with EPD, SPD and FBA. Our proposed scheme is applied to schemes discussed in the previous technology. Our proposed scheme does not need to know each connection's mean packet size. When the buffer exceeds the given threshold, it is based on comparison between the number of dropped packet and the approved packet. Our results are reported and discussed for comparing these discarding schemes under similar conditions. Although the number of virtual channel (VC) is increased, the simulation results showed that the proposed scheme can allocate more fairly each VC than other scheme.

• 수산해양기술연구
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• 제44권3호
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• pp.218-228
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• 2008

This paper describes on the real-time tracking of ship's dynamic behavior by AIS information in the coastal waters. The AIS data was received at a land station by using the antenna of AIS receiver mounted on the rooftop of the laboratory, Pukyong National University (PKNU), Busan, Korea, and stored as a NMEA format of serial output sentence of VDM(VHF Data-Link Message) and displayed on the ENC(Electronic Navigational Chart) of a PC-based ECDIS. In this study, the AIS receiver was mainly used to obtain the dynamic information that is necessary to evaluate and track the movement situation of training ship "KAYA" of PKNU in the coastal waters. The change of position with time for the ship turning under the rudder angle of port $30^{\circ}$ was correctly tracked with the turning circle of 940 m in diameter on the ENC of a PC-based ECDIS. Then, the dynamic information of the AIS system was updated every 6.29 seconds under the turning situation for the speed of 10.9 knots and every 21.65 seconds under the situation running at the speed of 11.05 knots on the straight line route of $155^{\circ}$, respectively. In case of AIS target tracking in the inshore zone behind large topographical obstructions, such as mountain and apartment buildings, the update rate of dynamic information was irregularly changed by the existence of land obstacles. However, the position tracking by AIS information under the situation existing no sea obstructions was achieved in real or near real-time and the instant presentation of course alternations for the ship was correctly monitored by using a PC-based ECDIS. From these results, we concluded that the PC-based ECDIS technology and methodology combined with the AIS information can be easily extended and applied to the surveillance and management for the fishing operation of fishing vessels in the coastal zone and in the EEZ fishing grounds.

• 대한원격탐사학회지
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• 제23권5호
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• pp.409-420
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• 2007

Since 1988, pine wilt disease has spread over rapidly in Korea. It is not easy to detect the damaged pine trees by pine wilt disease from conventional remote sensing skills. Thus, many possibilities were investigated to detect the damaged pines using various kinds of remote sensing data including high spatial resolution satellite image of 2000/2003 IKONOS and 2005 QuickBird, aerial photos, and digital airborne data, too. Time series of B&W aerial photos at the scale of 1:6,000 were used to validate the results. A local maximum filtering was adapted to determine whether the damaged pines could be detected or not at the tree level from high resolution satellite images, and to locate the damaged trees. Several enhancement methods such as NDVI and image transformations were examined to find out the optimal detection method. Considering the mean crown radius of pine trees, local maximum filter with 3 pixels in radius was adapted to detect the damaged trees on IKONOS image. CIR images of 50 cm resolution were taken by PKNU-3(REDLAKE MS4000) sensor. The simulated CIR images with resolutions of 1 m, 2 m, and 4 m were generated to test the possibility of tree detection both in a stereo and a single mode. In conclusion, in order to detect the pine tree damaged by pine wilt disease at a tree level from satellite image, a spatial resolution might be less than 1 m in a single mode and/or 1 m in a stereo mode.