• Journal of Applied Biological Chemistry
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• 제55권4호
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• pp.253-261
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• 2012

PCDD/Fs 및 DL-PCBs는 농식품 잔류 유해물질 중 높은 생물독성 및 생물농축성으로 안전성 우려가 높아 많은 연구자들이 관심을 갖는 유해물질들이다. 하지만, 대부분의 오염조사연구는 지방함유량이 높은 육류 및 수산물을 중심으로 이루어지고 있어, 다소비 농산물인 곡류를 대상으로 한 다이옥신 잔류분석 연구결과가 부족하여 식이섭취를 통한 다이옥신류 노출평가를 수행하는데 어려움이 있다. 본 논문에서는 17종의 PCDD/Fs 및 14종의 DL-PCBs에 대하여 2010년산 쌀과 2011년산 보리에 대한 잔류 분석 및 이를 통한 한국인의 일일 섭취노출량을 조사하였다. 전국 8개 도에서 채취한 36점의 백미시료와 전남 및 전북에서 채취한 7점의 보리시료에 대한 잔류분석결과 백미 중 다이옥신류의 각 도별 평균 잔류량은 2.98-4.98 pg/g w.w.이었으며, 보리의 다이옥신류 평균 잔류량은 3.00-3.24 pg/g w.w.로 확인되었다. 쌀과 보리를 통한 국민 1인당 다이옥신 섭취노출량은 1.03 pg-TEQ/day/person와 0.0534 pg-TEQ/day/person 수준이었고, 이를 한국인 평균체중 55 kg을 기준으로 환산할 경우 Lower bound 시나리오의 경우 TDI대비 0.46와 0.03%였으며, Upper bound 시나리오의 경우 5.5와 0.18%로 국내산 쌀과 보리를 통한 다이옥신류 노출기여도는 미미한 것으로 판단된다.

• 분석과학
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• 제24권6호
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• pp.484-492
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• 2011

우리나라 주요 하천수계의 대표적인 지점 한 곳씩 선정하여 이곳에 서식하는 붕어에 대한 PCDDs, PCDFs 및 DL-PCBs의 체내 축적 정도를 분석하고 분포특성을 살펴보았다. 회수율은 PCDDs 및 PCDFs는 50.6%~88.3%, DL-PCBs는 52.3%~93.2%이었으며, 붕어 체내에 축적된 PCDDs, PCDFs 및 DL-PCBs를 모두 합한 ${\sum}$dioxins은 평균 0.39 pg TEQ $g^{-1}$ wet wt., 중앙값 0.14 pg TEQ/g wet wt., 그리고 농도 범위는 0.047-1.0 pg TEQ/g wet wt.으로 나타났다. PCDDs 및 PCDFs는 일부 시료에서 검출된 반면, DL-PCBs는모든 시료에서 검출되었으며 ${\sum}$dioxins에 대한 기여도가 83.6%로서 가장 높았다. 다음으로 PCDFs와 PCDDs가 각각 ${\sum}$dioxins의 12.7%와 3.7%를 기여하고 있었다.

• Journal of Periodontal and Implant Science
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• 제43권3호
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• pp.130-135
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• 2013

Purpose: The purpose of this study was to evaluate the tensile strength of surgical synthetic absorbable sutures over a period of 14 days under simulated oral conditions. Methods: Three suture materials (polyglycolic acid [PGA], polyglactin [PG] 910, and poly (glycolide-co-${\epsilon}$-caprolactone) [PGC]) were used in 4-0 and 5-0 gauges. 210 suture samples (35 of each material and gauge) were used. All of the samples were tested preimmersion and 1 hour and 1, 3, 7, 10, and 14 days postimmersion. The tensile strength of each suture material and gauge was assessed. The point of breakage and the resorption pattern of the sutures were also assessed. Results: During the first 24 hours of immersion, all 4-0 and 5-0 samples of PGA, PG 910, and PGC maintained their initial tensile strength. At baseline (preimmersion), there was a statistically significant (P<0.001) difference in the tensile strengths between the 4-0 and 5-0 gauge of PGA, PG 910, and PGC. PGA 4-0 showed the highest tensile strength until day 10. At 7 days, all the 4-0 sutures of the three materials had maintained their tensile strength with PGA 4-0 having significantly greater (P=0.003) tensile strength compared to PG. Conclusions: 4-0 sutures are stronger and have greater tensile strength than 5-0 sutures. The PGA 4-0 suture showed the highest tensile strength at the end of day 10.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5651-5655
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• 2013

Objective: Major histocompatibility complex class I chain-related A (MICA) is a stress-inducible glycoprotein that can be shed as a soluble protein. This study was conducted to determine the expression of MICA in renal cell carcinoma (RCC) and examine the clinical relevance of soluble MICA (sMICA) in this disease. Methods: Immunohistochemistry and real-time PCR analyses were performed to assess the expression of MICA in 48 pairs of RCC and adjacent normal renal tissues. Serum levels of sMICA were measured in 48 RCC patients, 12 patients with benign renal tumors, and 20 healthy individuals. The correlations between sMICA levels and clinicopathological parameters were analyzed and the diagnostic performance of sMICA in RCC was evaluated. Results: RCCs exhibited elevated expression of MICA compared to adjacent normal tissues. Serum concentrations of sMICA were significantly greater in RCC patients ($348.5{\pm}32.5pg/ml$) than those with benign disease ($289.3{\pm}30.4pg/ml$) and healthy controls ($168.4{\pm}43.2pg/ml$) and significantly correlated with advanced tumor stage, lymph node metastasis, distant metastasis, vascular invasion, and higher histological grade. Using a cut-off point of 250 pg/ml, sMICA demonstrated a specificity and sensitivity of 63.2% and 75.6%, respectively, in distinguishing between RCC and benign renal tumors. Conclusion: MICA expression is upregulated in RCC and increased serum sMICA levels predict aggressive tumor behavior. However, the applicability of sMICA alone is limited in distinguishing RCC from benign renal tumors.

• 환경생물
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• 제38권3호
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• pp.343-349
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• 2020

본 연구에서는 한국 연안에 서식하는 대표적인 15종 패류의 핵DNA 함량을 조사하였다. 복족류에서 DNA 함량(pg DNA nucleus^-1 )은 3.3±0.08 (Haliotis discus hannai)과 2.4±0.18 (Batillus cornutus)이었다. 이매패류에서 DNA 함량(pg DNA nucleus^-1)은 2.0±0.15 (Scapharca broughtonii), 3.0±0.12 (Mytilus galloprovincialis), 2.9±0.05 (Meretrix lusoria), 2.2±0.03 (M. lamarkii), 2.6±0.05 (Fulvia mutica), 1.8±0.18 (Tegillarca granosa), 3.3±0.01 (Solen corneus), 2.2±0.04 (Barnea manilensis), 2.5±0.32 (Crassostrea gigas), 3.9±0.24 (Atrina pectinate), 3.5±0.15 (Patinopecten yessoensis), 1.9±0.16 (Amygdala philippinarum) 및 2.3±0.14 (Pseudocardium sachalinensis)이었다. 본 연구 결과는 본 연구에 사용된 패류의 genomic 진화과정을 더욱 잘 이해하는 새로운 정보를 제공한다.

• Journal of Periodontal and Implant Science
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• 제50권3호
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• pp.159-170
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• 2020

Purpose: Immunization with Porphyromonas gingivalis heat shock protein 60 (PgHSP60) may have an immunoregulatory effect on atherogenesis. The aim of this study was to determine whether nasal immunization with a PgHSP60 peptide could reduce atherosclerotic plaque formation in apolipoprotein E knockout (ApoE KO) mice. Methods: Seven-week-old male ApoE KO mice were assigned to receive a normal diet, a Western diet, a Western diet and challenge with PgHSP60-derived peptide 14 (Pep14) or peptide 19 (Pep19), or a Western diet and immunization with Pep14 or Pep19 before challenge with Pep14 or Pep19. Results: Atherosclerotic plaques were significantly smaller in mice that received a Western diet with Pep14 nasal immunization than in mice that received a Western diet and no Pep14 immunization with or without Pep14 challenge. An immunoblot profile failed to detect serum reactivity to Pep14 in any of the study groups. Stimulation by either Pep14 or Pep19 strongly promoted the induction of CD4⁺CD25⁺ forkhead box P3 (FoxP3)⁺ human regulatory T cells (Tregs) in vitro. However, the expression of mouse splenic CD4⁺CD25⁺FoxP3⁺ Tregs was lower in the Pep14-immunized mice than in the Pep14-challenged or Pep19-immunized mice. Levels of serum interferon gamma (IFN-γ) and transforming growth factor beta were higher and levels of interleukin (IL) 10 were lower in the Pep14-immunized mice than in the other groups. Induction of CD25^- IL-17⁺ T helper 17 (Th17) cells was attenuated in the Pep14-immunized mice. Conclusions: Nasal immunization with Pep14 may be a mechanism for attenuating atherogenesis by promoting the secretion of IFN-γ and/or suppressing Th17-mediated immunity.

• 한국가축번식학회지
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• 제14권3호
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• pp.165-173
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• 1990

A simple and sensitive microplate enzyme immunoassay(ELISA) was developed for progesterone, based on progesterone monoclonal antibody as anti-progesterone, horseradish peroxise(HRP) as enzyme-label and tetramethylbenzidine(TMB) as substrate. The assay has a sensitivity of 5pg-120pg/well and intra- and inter assay coefficients of variation for progesterone standard curve(0.1ng-3.2ng/ml) were ranged 4.4-10.6% and 5.-12.6%, respectively. They assay is performed in less than two hours and provide reliable values to differentiate among samples from day 0(A.I.), day 14 and day 19. The discriminatory levels for early pregnancy diagnosis are [>10ng(day 19) & decreasing rate <1.5 : pregnancy] and [ 7ng & decreasing rate 1.5 : non-pregnancy]. The accuracy of the pregnancy diagnosis for cows classified as positive(pregnancy) and negative(non-pregnancy) were 96% and 100%, respectively.

• Mass Spectrometry Letters
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• 제14권2호
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• pp.25-35
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• 2023

Plants are a traditional source of many chemicals used as biochemical, flavors, food, color, and pharmaceuticals in various countries, especially India. Most herbal medicines and their derivatives are often made from crude extracts containing a complex mixture of various phytochemical chemical components (secondary metabolites of the plants). This study aimed to identify bioactive compounds from the different parts of the plant from the ethanolic extract of Gymnema sylvestre, Senna auriculata, and Cissus quadrangularis (leaves, flower, stem) by gas chromatography-mass spectroscopy (GC-MS). The gas chromatography - mass spectrometry analysis revealed the presence of various compounds like 3,4-dimethylcyclohexanol, hexanoic acid, D-mannose, and N-decanoic acid. Hence, the Gymnema sylvestre, Senna auriculata, and Cissus quadrangularis may have chemopreventive, anti-cancer, anti-microbial activity, antioxidant, anti-diabetic activity, anti-inflammatory, and antifungal due to the presence of secondary metabolites in the ethanolic extract. These phytochemicals are supported for traditional use in a variety of diseases.

• 대한수의학회지
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• 제63권2호
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• pp.14.1-14.4
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• 2023

This study evaluated the effect of resynchronization programs on pregnancies in dairy cows. Of 1,342 cows confirmed not pregnant after their first artificial insemination (AI), those with a corpus luteum (CL) were resynchronized using Ovsynch or PG-GnRH-Ovsynch and those without a CL were resynchronized using GnRH-Ovsynch or modified Double-Ovsynch. There were no differences (p > 0.05) in the pregnancies per AI either between the Ovsynch (31.3%) and PG-GnRH-Ovsynch (34.0%) or between the GnRH-Ovsynch (38.7%) and modified Double-Ovsynch (39.5%). In conclusion, Ovsynch and GnRH-Ovsynch programs could be preferred to resynchronize cows with and without a CL, respectively, from the perspective of reducing costs and labor.

• Mass Spectrometry Letters
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• 제14권3호
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• pp.79-90
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• 2023

Natural goods, especially therapeutic plants, are abundant in the World. Because they have the ability to provide all humanity with countless advantages as a source of medicines, medicinal plants are presently receiving more attention than ever. These plants' therapeutic efficacy is based on bioactive phytochemical components that have clear physiological effects on the human body. The drying process is crucial for the preparation of plant materials prior to extraction since freshly harvested plant materials include active enzymes that create active components, intermediates, and metabolic processes. Many of the phytoconstituents may be extracted using the semi-polar solvent methanol. The goal of the current work is to use the GC-MS gas chromatography- mass spectrometry technology to identify the phytochemicals and review their biological activity. In methanol leaf extract, 5 phytocompounds were found in Ricinus communis, 5 phytocompounds in Pongamia pinnata, 12 phytocompounds in Datura metal, 7 phytocompounds in Azadirachta indica, 11 phytocompounds in Acalypha indica.