• 한국응용곤충학회지
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• 제35권1호
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• pp.66-73
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• 1996

Bacillus thuringiensis subsp. morrisoni PG-14의 cryIVD 유전자를 포함하고 있는 발현벡터 pCYASK5-1을 제작하여 Synechocystis PCCC6803에 형질전환시킨 후, 학질모기(Anopheles sinensis)유충에 대한 독성을 검정하였다. Kanamycin이 포함된 BG-11배지에서 선발된 형질전환체의 cryIVD 유전자 발현은 SDS-PAGE와 West-ern blot분석으로 확인하였다. 형질전환체는 A. sinensis 유충에 대해 높은 독성을 나타내었으며, 성장은 야생주인 Synechocystis Pccc6803과 유사하였다. 또 수심에 따른 형질전환체의 분포도 조사에서 전체적으로 살충농도의 세포수로 분포함을 확인하였다. 이러한 결과들은 B. thuringiensis subsp. morrisoni PG-14의 cryIVD 유전자로 형질전환된 Synechocystis PCCC6803이 모기유충 방제에 효율적으로 이용될 수 있음을 나타내었다.

• 대한한방부인과학회지
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• 제21권4호
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• pp.139-149
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• 2008

Purpose: The purpose of this study was to examine the relationships between variety pregnancy related factors and postpartum BMI change. Methods: Analyzing the BMI of 125 postpartum women in oriental medical hospital of O O university from March, 2008 to May, 2008. Age, pregnancy period. type of delivery, parity, pregravida(PG) BMI, weight and BMI gain during pregnancy, gestational maximum(GMx) BMI were recorded. BMI also checked on each postpartum 7days(P7d), 14days(P14d), 90days(P90d), And we distinguished into Sasang Constitution by QSCC II. Results: The following results were obtained: 1. Age. pregnancy period were not correlated to postpartum BMI change. 2. PG BMI were lower in normal delivery group than caesarean delivery group. 3. PG BMI was lower in primipara group than multipara group. Weight and BMI gain during pregnancy. P90d BMI were lower in multipara group. 4. PG, GMx, P7d, P14d, P90d BMI were significally high in Taeumin. 5. PG BMI were correlated to GMx, P7d, P14d, P90d BMI. 6. Weight and BMI gain during pregnancy were correlated to GMx BMI and P7d, P14d, P90d BMI, BMI loss. Conclusion: This results suggested that weight and BMI gain during pregnancy have the best relationship with postpartum weight change.

• 분석과학
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• 제14권6호
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• pp.504-509
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• 2001

우리 나라 사람들의 체지방 조직과 초유중에서, 다이옥신의 잔류 농도를 HRGC/HRMS를 이용하여 동위원소희석법으로 측정하였다. 체지방 조직 시료는 서울에 있는 모병원에서 채취하였으며, 체지방 조직에서의 PCDFs의 총농도와 TEQ 농도는 각각 5.812 pg/g, 1.485 pgTEQ/g 이었고, PCDDs의 총농도와 TEQ 농도는 각각 26.648 pg/g, 1.176 pgTEQ/g 이었다. 서울대학 병원으로부터 받은 산모 20명의 초유에 함유된 다이옥신의 잔류농도는 PCDFs의 총 농도가 7.019 pg/mL, TEQ 농도로 환산하면 0.177 pgTEQ/mL 이었고, PCDDs의 총 농도와 TEQ 농도는 각각 14.224 pg/mL, 0.693 pgTEQ/mL이었다. 체지방 조직과 모유에서의 다이옥신 이성질체들의 분포는 PCDDs가 PCDFs보다 상대적으로 높게 나타났으며, 그중 OCDD가 가장 높은 농도로 포함되어 있다.

• 생명과학회지
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• 제19권3호
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• pp.334-342
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• 2009

본 연구는 streptozotocin (STZ)으로 당뇨를 유발한 실험쥐에 대한 Psidium guajava L. 잎(Pg), Lagerstroemia speciosa L. 잎(Ls) 추출물 그리고 혼합물 A (Pg, Ls, Morus indica L. 잎 추출물, Pinus densiflora needles 추출물, Acanthopanax senticosus M. roots 추출물)의 효과를 조사하였다. 4주간 streptozotocin (STZ)으로 당뇨를 유발한 실험쥐에 이들 추출물을 섭취시킨 결과 Pg, Ls 그리고 혼합물 A의 섭취는 당뇨 대조군에 비해 혈당을 각각 20%, 14% 그리고 24% 감소시켰다. 또한 이들 추출물의 섭취는 총 콜레스트롤, 중성지방, 유리지방산을 감소시켰고 인슐린과 HDL-콜레스트롤을 효과적으로 증가시켰다. 결론적으로 혼합물 A는 Pg와 Ls 추출물에 비해 혈당 및 지질 감소, 인슐린 증가 효과 그리고 기능적으로 손상된 베타세포의 회복이 더 높은 것으로 나타났다. 따라서 혼합물 A는 개별적으로 사용한 Pg와 Ls 추출물에 비해 더욱 유익한 상승효과를 발휘하는 것으로 증명되었다.

• ETRI Journal
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• 제8권3호
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• pp.3-14
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• 1986

Currently, CIF is used widely as a common data base to represent IC layouts. The CIF2PG program is developed using C language to obtain electromask or mann 3000 PG file from the CIF input file. By obtaining the PG data, which can realize the actual mask layout, the construction of IC Design Automation System became possible using a general purpose computer.

• Journal of Ginseng Research
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• 제31권1호
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• pp.54-59
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• 2007

In this paper, we present evidence that the Korean red ginseng extract shows the immunomodulatory activities during postoperative chemotherapy after curative surgery in patients with advanced colon cancer. We measured the circulating interleukin-2 (IL-2), interleukin-8 (IL-8)and interleukin-10 (IL-10) as a immune modulator to evaluate the effect of Korean red ginseng. The mean preoperative value of IL-2 was similar in the non-RG group and the RG group (5.72 pg/ml versus 6.87 pg/ml, p>0.05). The mean value of IL-2 was compared with IL-2 from healthy control group, there was no significant difference (14.89 pg/ml versus 14.22 pg/ml, p>0.05). The mean preoperative value of IL-8 was higher in the non-RG group comparing with the RG group (30.92 pg/ml versus 36.25 pg/ml, p < 0.05). At postoperative 3 month, the mean values of IL-8 from non-RG and RG group down to 24.56 pg/ml and 21.46 pg/ml respectively. The IL-8 of RG group at 3 month showed no difference with that of HC group(21.46 pg/ml versus 16.31 pg/ml, p>0.05). The preoperative mean value of IL-10 of non-RG, RG and HC group was 11.56 pg/ml, 10.8 pg/ml, and 3.68 pg/ml respectively. At postoperative 3 month, the mean values of IL-10 from non-RG and RG group down to 8.45 pg/ml and 5.04 pg/ml respectively. In spite of decreasing IL-10 levels of both cancer Patients group with time, there was still significant difference with that of HC group (non-RG versus HC group, p=0.00, RC versus HC group, p=0.04). The results of this study suggest that the red ginseng extract may have some immunomodulatory properties associated with IL-2, IL-8 and IL-10 activity in patients with advanced colorectal cancer during postoperative chemotherapy. We think to need the further studies and a larger sample size to fully evaluate the antitumor effect of ginseng and need to establish this mechanism of action as well as identify the active components associated with antitumor activity and immunomodulation in patients with advanced colorectal cancer.

• Journal of Gastric Cancer
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• 제14권4호
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• pp.246-251
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• 2014

Purpose: There are two surgical procedures for proximal early gastric cancer (EGC): total gastrectomy (TG) and proximal gastrectomy (PG). This study aimed to compare the long-term outcomes of PG with those of TG. Materials and Methods: Between January 2001 and December 2008, 170 patients were diagnosed with proximal EGC at Soonchunhyang University Cheonan Hospital, of which 64 patients underwent PG and 106 underwent TG. Clinicopathologic features, postoperative complications, blood chemistry data, changes in body weight, and oncological outcomes were analyzed and retrospectively compared between both groups. Results: Tumor size was smaller and the number of retrieved lymph nodes was lower in the PG group. The postoperative complication rate was 10.9% in the TG group and 16.9% in the PG group. The incidence of Los Angeles grade C and D reflux esophagitis was significantly higher in the TG group. Hemoglobin level was higher and body weight loss was greater in the TG group at 2, 3, and 5 years postoperatively. The albumin levels at 3 and 5 years were lower in the TG group. There was no significant difference in the 5-year overall survival rates between the two groups (P=0.789). Conclusions: Postoperative complications and oncologic outcomes were observed to be similar between the two groups. The PG group showed better laboratory data and weight loss than did the TG group. Moreover, severe reflux esophagitis occurred less frequently in the PG group than in the TG group. PG can be considered as an effective surgical treatment for proximal EGC.

• Mass Spectrometry Letters
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• 제14권1호
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• pp.9-23
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• 2023

Plants are able to produce a large number of diverse bioactive compounds. Solvent extraction is used for isolation of plant metabolites. The extract yield for plant metabolite extraction strongly depends on the nature of solvent. A review showed the methanol can yield more bioactive compounds. Drying of the sample material is also important for the extraction of plant material. The present study was carried out to analyze the phytocomponents of 5 different latex producing plants. The plants like Calotropis gigantea, Carica papaya, Nerium oleander, Ficus benghalensis and Plumeria alba leaves and latex. The GC-MS analysis of the metabolites revealed phytocomponents. Calotropis gigantea leaves showed 14 compounds and latex produced 5 compounds out of this 4,4,6A,6B,8A,11,11,14B-Octamethyl-1,4,4A,5,6,6A,6B,7,8,8A,9,10,11,12,12A,14,14A,14B-Octadeca-hydro-2 and 2R- Acetoxymethyl-1,3,3-trimethyl-4T-(3-Methyl-2-Buten-1-Yl)-1T-Cyclohexanol compound was present in both latex and leaf extraction. Beta. -carotene compound was present in both latex and leaf of Carica papaya. It was observed that Ficus benghalensis contained 2R-Acetoxymethyl-1,3,3-trimethyl-4T-(3-Methyl-2-Buten-1-Yl)-1T-Cyclohexanol was same in latex and leaf extraction.

• 한국미생물·생명공학회지
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• 제24권2호
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• pp.173-177
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• 1996

Bacillus thuringiensis subsp. morrisoni PG-14 is a gram-positive soil bacterium producing mosquitocidal parasporal inclusions composed of several crystal proteins. Among these crystal protein genes, cryIVD gene is one of major component which has 72 kDa in size. However, these parasporal inclusions sink quickly from the surface of water where mosquito larval feeding occurred. To develope mosquitocidal cyanobacterium, therefore, we constructed the expression vector, pCYASK 5-1 harboring cryIVD gene. The expression vector, pCYASK5-1 was transformed into the cyanobacterium Syne- chocystis PCC6803 reported as a natural mosquito larval food source and the transformants were selected with kanamycin. Expression of IVD gene in transformant was characterized by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined with Culex tritaeniorhynchus. The results showed that, the transformed cyanobacterium is toxic to mosquito larvae and will be expected as a potential agent that is used for mosquito control.

• 분석과학
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• 제23권6호
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• pp.595-606
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• 2010

4년 동안(2004-2007) 국내 유통중인 식품 총 221건을 구입하여 다이옥신류(PCDD/DF + 다이옥신유사 PCBs)를 동위원소희석법을 이용하여 분석하였고, 한국인의 다이옥신 1일 노출량을 산출하였다. 식품 중 다이옥신류의 검출량은 2004년과 2005년은 오염도 자료에 1998 WHO TEF값을, 2006년부터는 오염도 자료에 2005 WHO TEF값을 사용하여 다이옥신류 함량을 산출하였으며, 평균 검출량은 백미는 < 0.01 pg TEQ/g, 소고기 0.13 pg TEQ/g, 돼지고기 0.07 pg TEQ/g, 닭고기 0.04 pg TEQ/g, 우유 0.04 pg TEQ/g, 치즈 0.05 pg TEQ/g, 계란(노른자)는 0.13 pg TEQ/g, 수산물 중 고등어는 1.35 pg TEQ/g, 삼치 1.03 pg TEQ/g, 갈치 1.00 pg TEQ/g, 조기 0.16 pg TEQ/g, 명태 0.11 pg TEQ/g, 장어 0.52 pg TEQ/g, 굴 0.32 pg TEQ/g, 꽃게 0.14 pg TEQ/g 수준으로 조사되었다. 식품을 통한 한국인의 다이옥신류의 노출량은 체중 1 kg 당 1일 0.47 pg TEQ로 다이옥신류의 TDI (4 pg TEQ/kg bw/day) 대비 약 11%수준으로 조사되었으며, 이 수준은 건강상 위해가 없는 것으로 평가되었다.