• The Korean Journal of Physiology and Pharmacology
• /
• v.3 no.5
• /
• pp.513-519
• /
• 1999

Direct exposure of renal tubular brush-border membranes (BBM) to free cadmium (Cd) causes a reduction in phosphate (Pi) transport capacity. Biochemical mechanism of this reduction was investigated in the present study. Renal proximal tubular brush-border membrane vesicles (BBMV) were isolated from rabbit kidney outer cortex by Mg precipitation method. Vesicles were exposed to $50{\sim}200\;{\mu}M\;CdCl_2$ for 30 min, then the phosphate transporter activity was determined. The range of Cd concentration employed in this study was comparable to that of the unbound Cd documented in renal cortical tissues of Cd-exposed animals at the time of onset of renal dysfunction. The rate of sodium-dependent phosphate transport $(Na^+-Pi\;cotransport)$ by BBMV was determined by $^{32}P-Iabeled$ inorganic phosphate uptake, and the number of $Na^+-Pi$ cotransporters in the BBM was assessed by Pi-protectable $^{14}C-labeled$ phosphonoformic acid $([^{14}C]PFA)$ binding. The exposure of BBMV to Cd decreased the $Na^+-Pi$ cotransport activity in proportion to the Cd concentration in the preincubation medium, but it showed no apparent effect on the Pi-protectable PFA binding. These results indicate that an interaction of renal BBM with free Cd induces a reduction in $Na^+-Pi$ cotransport activity without altering the carrier density in the membrane. This, in turn, suggest that the suppression of phosphate transport capacity $(V_{max})$ observed in Cd-treated renal BBM is due to a reduction in $Na^+-Pi$ translocation by existing carriers, possibly by Cd-induced fall in membrane fluidity.

• Imaging Science in Dentistry
• /
• v.40 no.4
• /
• pp.171-178
• /
• 2010

Purpose : The purpose of this study is to develop a surgical stent for dental implant procedure that can be easily applied and affordable by using cone beam computerized tomography (CBCT). Materials and Methods : Aluminum, Teflon-PFA (perfluoroalkoxy), and acetal (polyoxymethylene plastic) were selected as materials for the surgical stent. Among these three materials, the appropriate material was chosen using the CBCT images. The surgical stent, which could be easily placed into an oral cavity, was designed with chosen material. CBCT images of the new surgical stent on mandible were obtained using Alphard-3030 dental CT system (Asahi Roentgen Co., Ltd., Kyoto, Japan). The point of insertion was prescribed on the surgical stent with the multiplanar reconstruction software of OnDemand3D (CyberMed Inc., Seoul, Korea). Guide holes were made at the point of insertion on the surgical stent using newly designed guide jig. CBCT scans was taken for the second time to verify the accuracy of the newly designed surgical stent. Results : Teflon-PFA showed radiologically excellent image characteristics for the surgical stent. High accuracy and reproducibility of implantation were confirmed with the surgical stent. Conclusion : The newly designed surgical stent can lead to the accurate implantation and achieve the clinically predictable result.

• Elastomers and Composites
• /
• v.45 no.3
• /
• pp.212-216
• /
• 2010

The materials of the lithium ion battery gasket require chemical resistance to the electrolyte, electrical insulating, compression set, anti-contamination and heat resistance. To estimate suitability for rubber which has better performance to compression set than PFA, each compound were made with various rubbers, such as EPDM, NBR, FKM, FVMQ, VMQ and we checked the characteristics of each compound. Samples from each compound was deposited in Propylene Carbonate and tested for changing of Hardness and Volume during 1,000 hr with $80^{\circ}C$. EPDM and VMQ showed good performance to chemical resistance to the electrolyte, and also we could get the values over $10^{10}{\Omega}cm$ on volume resistance basis in electrical insulating. EPDM and VMQ were judged as the most suitable material.

• Polymer(Korea)
• /
• v.29 no.1
• /
• pp.96-101
• /
• 2005

Fluoropolymer of PTFE and PFA etc. is a heat resistance polymeric material that it is known as that maximum continuous use temperature reaches for 260 $^{\circ}C$. It was observed that these polymers had the enough thermal stability so enough that it was kept by thermal aging of 280 $^{\circ}C$/7 weeks too in this study. However, such thermal stability means that bulk material property is kept such as mechanical strength, melting point and initial pyrolysis temperature etc. If these polymers are evaluate by coating property such as surface contact angle, surface morphology, surface scratch, thing that heat resistance is not enough was confirmed in this study. Thermal aging of flouropolymer coating was achieved by gear aging oven that the exchange rate of air was controlled, and the analysis results were indicating serious damage of surface morphology and adhesive strength on metal substrate.

• Journal of the Architectural Institute of Korea Structure & Construction
• /
• v.36 no.3
• /
• pp.121-128
• /
• 2020

In this paper, the ASCE 7 equivalent static approach for seismic design of non-structural elements is critically evaluated based on the measured floor acceleration data, theory of structural dynamics, and linear/nonlinear dynamic analysis of three-dimensional building models. The analysis of this study on the up-to-date database of the instrumented buildings in California clearly reveals that the measured database does not well corroborate the magnitude and the profile of the floor acceleration as proposed by ASCE 7. The basic flaws in the equivalent static approach are illustrated using elementary structural dynamics. Based on the linear and nonlinear dynamic analyses of three-dimensional case study buildings, it is shown that the magnitude and distribution of the PFA (peak floor acceleration) can significantly be affected by the supporting structural characteristics such as fundamental period, higher modes, structural nonlinearity, and torsional irregularity. In general, the equivalent static approach yields more conservative acceleration demand as building period becomes longer, and the PFA distribution in long-period buildings tend to become constant along the building height due to the higher mode effect. Structural nonlinearity was generally shown to reduce floor acceleration because of its period-lengthening effect. Torsional floor amplification as high as 250% was observed in the building model of significant torsional irregularity, indicating the need for inclusion of the torsional amplification to the equivalent static approach when building torsion is severe. All these results lead to the conclusion that, if permitted, dynamic methods which can account for supporting structural characteristics, should be preferred for rational seismic design of non-structural elements.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.13 no.1 s.53
• /
• pp.97-105
• /
• 2009

Development and use of blended cement concrete is gaining more importance in the construction industry with reference to durability mainly due to the pore refinement and reduction in permeability. Cracks play a major role on important parameters like permeability, rate of chloride ingress, compressive strength and thus affect the reinforcement corrosion protection. Furthermore, when a crack occurs in the cover concrete, the corrosion of the steel reinforcement may be accelerated because the deterioration causing factors can pass through the crack. In recent years the effect of cracking on the penetration of concrete has been the subject of numerous investigations. Therefore assessing the service life using blended concrete becomes obviously in considering the durability. In the present study, the corrosion assessment of composite concrete beams with and without crack with of 0.3mm using OPC, 30% PFA, 60% GGBS, 10% SF was performed using half cell potential measurement, galvanic potential measurement, mass loss of steel over a period of 60days under marine environmental conditions and the results were discussed in detail.

• Journal of the Korea Concrete Institute
• /
• v.21 no.3
• /
• pp.245-253
• /
• 2009

The importance of chloride ions in the corrosion of steel in concrete has led to the concept for chloride threshold level (CTL). The CTL can be defined as the content of chlorides at the steel depth that is necessary to sustain local passive film breakdown and hence initiate the corrosion process. Despite the importance of the CTL, due to the uncertainty determining the actual limits in various environments for chloride-induced corrosion, conservative values such as 0.4% by weight of cement or 1.2 kg in 1 $m^3$ concrete have been used in predicting the corrosion-free service life of reinforced concrete structures. The paper studies the CTL for blended cement concrete by comparing the resistance of cementitious binder to the onset of chloride-induced corrosion of steel. Mortar specimens were cast with centrally located steel rebar of 10 mm in diameter using cementitious mortars with ordinary Portland cement (OPC) and mixed mortars replaced with 30% pulverized fuel ash (PFA), 60% ground granulated blast furnace slag (GGBS) and 10% silica fume (SF), respectively, at 0.4 of a free W/B ratio. Chlorides were admixed in mixing water ranging 0.0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.5, 2.0, 2.5 and 3.0% by weight of binder(Based on $C1^-$). Specimens were curd 28 days at the room temperature, wrapped in polyethylene film to avoid leaching out of chloride and hydroxyl ions. Then the corrosion rate was measured using the polarization resistance method and the order of CTL for binder was determined. Thus, CTL of OPC, 60%GGBS, 30%PFA and 10%SF were determined by 1.6%, 0.45%, 0.8% and 2.15%, respectively.

• Applied Biological Chemistry
• /
• v.41 no.8
• /
• pp.583-586
• /
• 1998

Porous ceramic cups are used for monitoring ion concentration in soil solutions in various time course and depth. A soil solution sampler was constructed in laboratory by inserting pliable perfluoroalkoxy(PFA) tubings into porous cup through holes in PVC rod segment which plugged top opening of the porous cup. The system was installed in drip irrigated soil in a vertical position, and nitrogen movement below the drip basin was monitored. To collect soil solution, vacuum in the cup was applied with a hand vacuum pump. The samples obtained were sufficient enough to run quantitative analyses for a number of chemicals. Nitrogen transformation and movement could be well defined, and the system seemed to be relevant to the other soil solution samplers in monitoring chemical movement in soil. Although this system has general deficiencies found in the other samplers using ceramic cup, it could be easily constructed at a low cost. Since the tubing was pliable, the cups could be installed in horizontal position, and this allows installations of the cups at more precise depth increments and also more precise samplings of soil solution at each depth.

• Proceedings of the KSAR Conference
• /
• 2004.06a
• /
• pp.271-271
• /
• 2004

본 실험은 TGF-a를 처리하여 분화가 유도된 인간배아 줄기세포를 파킨슨 동물모델에 이식하여 숙주세포에서의 생존 및 이식효과를 검토하고자 실시하였다. TGF-a로 분화된 세포의 이식효과를 판정하고자 배양시 TGF-a처리군과 처리하지 않은 군으로 나누어 분화를 유도한 인간배아 줄기세포를 hoechst33342로 표지 하여 병변 유발과 동일한 방법으로 동측 선조체내에 4×10⁴개/2ul가 되도록 이식하고(이식 위치: AP 0.7, ML 2.0, DV3.4) 이식 후 2, 4주에서 행동학적 변화를 관찰하고 4주에 동물을 희생시켜 4% PFA를 이용하여 뇌 조직을 고정하고 뇌 조직은 40㎛ 두께로 동결 절편을 만들어 면역조직화학염색을 시행하여 신경세포로의 분화 및 TH 발현 여부를 관찰하였고 분화의 표지물질로 nestin, NF200, GFAP, TH를 사용하여 형태학적 변화를 관찰하였다. (중략)

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2002.11b
• /
• pp.492-497
• /
• 2002

In this paper, Power Flow Analysis(PFA) method has been applied to the prediction of vibration energy density and intensity of coupled shell structures in the medium-to-high frequency ranges. To consider the wave transformation at joint between shell elements, power transmission and reflection coefficients are investigated for various joint angles, and here Donnell-Mushtari thin shell theory has been used. For validations computations are performed to analyze the response of coupled shells by changing the excitation frequency and damping loss factor.