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Systematic Study of Fluorescein-Functionalized Macrophotoinitiators for Colorimetric Bioassays

  • Lee, Jeong-Gyu;Han, Gyeong-Yeop;Go, Sang-Won;Sikes, Hadley D.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.263.2-263.2
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    • 2013
  • We report a systematic investigation of a set of macrophotoinitiators for use in polymerization-based signal amplification. To test the dependence of photopolymerization responses on the number of photoinitiators localized per molecular recognition event, we gradually increased the number of photoinitiator molecules coupled to a scaffold macromolecule. Macrophotoinitiators constructed with an average of 7 to 168 photoinitiators per polymer with the goals of quantifying the relationship between the number of initiators per binding event and the degree of amplified colorimetric readout. To evaluate the capacity of the macrophotoinitiators to detect molecular recognition, neutravidin was coupled to these molecules to recognize biotin-labeled DNA immobilized on biochip test surfaces. Fluorescein macroinitiators are found to be useful in detecting molecular recognition above a threshold of initiators per polymer. Above this threshold, increasing the number of initiators per macroinitiator resulted in increased signal strength.

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Evaluation of Dietary Variety by Food Habits in Female University Students (여대생의 식습관에 따른 식사 다양성 평가)

  • Lee, Bo-Kyung;Lee, Jong-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.24 no.4
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    • pp.687-701
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    • 2011
  • The purpose of this study was to assess dietary variety by food habits(regularity of meal time, regularity of repast, repast of breakfast, lunch, and supper, number of suppers and night snacks per week, and duration of meal) in 138 female university students residing in Bucheon and its adjacent area. Food habits were assessed via a self reporting questionnaire, and a 3-day dietary recall survey was conducted by interviewing. Dietary variety was assessed by dietary diversity score(DDS), meal balance score(MBS), and dietary variety score(DVS). The average DDS, MBS, and DVS were $3.87{\pm}0.57$, $7.27{\pm}1.48$, and $12.59{\pm}3.14$, respectively. The DDS($2.21{\pm}0.83$) of the breakfast in the group in which the regularity of meal time per week was 5~7 days was significantly higher($p$ <0.01) than the DDS($1.47{\pm}0.96$) of the breakfast in the group in which the regularity of meal time per week was ${\leq}$2 days. The MBS($6.69{\pm}1.43$) of the group in which the regularity of meal times per week was ${\leq}2$ days was significantly lower($p$ <0.01) than the MBS of the group in which the regularity of meal time per week was ${\geq}3$ days. The DDS($2.38{\pm}0.69$) of the breakfast in the breakfast over eating group was significantly higher($p$ <0.001) than the DDS($1.83{\pm}0.83$) of breakfast under eating group. The MBS($6.56{\pm}1.46$) in the breakfast skipping group was significantly lower($p$ <0.01) than the MBS(about 7.6) in other breakfast eating groups, and the DVS ($13.79{\pm}3.21$) in the breakfast over eating group was significantly higher($p$ <0.05) than the DVS($11.53{\pm}2.94$) in the breakfast skipping group. The DDS of breakfast, lunch, and snack were not significantly correlated with the number of suppers per week, but the DDS($2.65{\pm}0.52$) of the supper in the group in which the number of suppers per week was 7 was significantly higher($p$ <0.05) than the DDS($2.22{\pm}0.49$, $2.25{\pm}0.62$) of the supper in the group in which the number of suppers per week was ${\leq}2$, 3~4. The DDS, MBS and DVS were not significantly correlated with the number of suppers per week. The DDS($1.33{\pm}1.25$) of the breakfast in the group in which the number of night snacks per week was 6~7 was significantly lower($p$ <0.05) than the DDS of the breakfast in the group in which the number of night snacks was ${\leq}5$. Also, the DDS($4.42{\pm}0.32$) per day in the group in which the duration of meal was <10 min. was significantly higher($p$ <0.01) than the DDS($3.51{\pm}0.53$) per day in the group in which the duration of meal was ${\geq}30$ min. These findings suggest that nutritional education based on female university students' eating variety and food habits(regularity of meal time, regularity of repast, repast of breakfast, lunch, and supper, number of suppers, and night snacks per week, and duration of meal) may be required to improve dietary variety.

Studies on Reproductive Capacity of Korean Native Bulls I. Effects of Age, Season and Collection Frequency on Semen Volume, Sperm Concentration, Total Sperm and Sperm Motility in Bulls (한우 종모우의 번식능력에 관한 연구 1. 종모우의 연령, 계절 및 채취빈도가 정액량, 정자농도, 총정자수 및 정자활력에 미치는 영향)

  • 김선환;김기구;유규종;고석권;김경주;김충기;김창근;정영채
    • Korean Journal of Animal Reproduction
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    • v.5 no.2
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    • pp.35-42
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    • 1981
  • A total 2,488 ejaculates during 4 years from 80 Korean native bulls over the age from 3 to 12 years of bull herds of Artificial Breeding Center, National Livestock Federatives Cooperation were collected and analyzed to study the effects of collection year, age of bulls, month of years, collection interval and ejaculation frequency per day on semen volume, sperm concentration, total sperm per ejaculate and sperm motility. 1. Semen volume, sperm concentration, total sperm per ejaculate and sperm motility index for the all ejaculates using least squares procedure averaged 5.73ml, 9.133${\times}$108/ml, 52.527${\times}$108 and 62.81, respectively. 2. Semen volume varied significantly with collection year, collection interval and ejaculation frequency per day(P<0.01), but effects of age of bulls and month of years on semen volume were not significant. Eight to 15 days collection interval showed the highest volume, and 1st ejaculate contained 5.6% more volume than 2nd ejaculate. 3. There were significant differences among collection years, months of years and ejeculation frequencies per day except collection intervals in sperm concentration per ml(P<0.05, P<0.01). Six to 8-year-old bulls was the highest concentration. Higher sperm concentration per ml was in April to July and lower month was October and December. Sperm concentration in 2nd ejaculate was higher than in 1st ejaculate. 4. Total sperm per ejaculate affected by all environmental factors studied(P<0.05, P<0.01). Age of bulls, collection interval and ejaculation frequency per day showed the highest total sperm was 6 to 8-year-old bulls, 8 to 15 days interval and 1st ejaculate, respectively. Higher total sperm per ejaculate was in April to July and lower total sperm was in September to December. 5. In sperm motility, there were significant differences among collection years, ages of bulls and collection intervals except months of years and ejaculation frequencies (P<0.01). Higher sperm motility was in 6 to 12-year-old bulls and in 5 to 7 days collection interval.

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A Multiple Planting in a Hole for Producing an Aromatic Tobacco Variety, Sohyang(Nicotiana tabacum L.) (향끽미품종담배의 식혈간 거리와 식혈당 주수가 수량 및 품질에 미치는 영향)

  • 정기택;변주섭
    • Journal of the Korean Society of Tobacco Science
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    • v.3 no.1
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    • pp.58-65
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    • 1981
  • This study was carried out to investigate the influence of distance of planting holes (51, 45, and 40 cm) and number of plants per a hole(4, 5, 6 plants) on agronomic characteristics, yield, and quality of an aromatic tobacco, Sohyang. The results are as follows: 1. Relative light intensity increased by widening the distance of holes. 2. Leaf area per a plant or per a leaf, and leaf length and width increased by widening the distance of holes and decreased by increasing the number of plants per a hole. But L. A. 1. increased by increasing the number of plants per a hole. Leaf shape index (Leaf length/Leaf width) showed little differences among treatments. 3. Dry weight of leaf, root, and stem per a plant decreased by increasing the number of plants per a hole. 4. Total nitrogen decreased by increasing number of plants per a hole and in the case of narrow distance of holes, but nicotine, reducing sugar, ether-extract and ash showed little differences. 5. Yield per 10a decreased by widening the distance of holes. 6. Quality(price per kg) was improved by increasing the number of plants per a hole at the Plot of 51m distance of holes. But there was no variation at the Plot of 45cm. And quality was decreased at the plot of 40cm distance of holes by increasing the number of Plants per a hole. 7. Price per 10a was highest in the plot of which plant spacing was $90\times$40cm and the number of plants per a hole was 4 (11112 plant/10a).

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A Follow-up Survey on the Socio-medical Status of the Drop-out Cases of Tuberculosis in Jeonju Health Center (보건소등록결핵환자(保健所登錄結核患者)에 대(對)한 사회의학적(社會醫學的) 조사연구(調査硏究))

  • Kim, Jong-Soon
    • Journal of Preventive Medicine and Public Health
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    • v.7 no.1
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    • pp.123-130
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    • 1974
  • A socio-medical survey was carried out on 1,108 cases of pulmonary tuberculosis who had registered and 220 cases unregistered at Jeonju health center in 1973, during from June 1 to July 31, 1974. As the results of this survey, the following conclusions were obtained. 1. Of the total 1,108 cases of pulmonary tuberculosis there were 708 new cases, while remaining 400 were old cases and rate of registration was 4.0 per thousand person. 2. The highest rate of registration of the newly diagnosed pulmonary tuberculosis could be found in bacteriological examination while 54.4 per cent in X-ray examination. 3. As for the educational status of the tuberculosis patients, primary school graduates contituted the greatest proportion or 64.6 per cent, middle school graduates 13.6 per cent, high school graduates 6.8 per cent and collge graduates only 0.7 per cent. 4. By age group of the cases, at age of 20 to 29 years occupied 23.6 per cent (262 out of 1,108 cases), 17.9 per cent at age of 40 to 49 years. 5. The greatest proportion or 38.4 per cent of the cases had no occupation. 6. The living standard of the patient's household, low class constituted 60.6 per cent of the total households. 7. By distribution of residential area, farming area was 5.0 per thousand person, 4.0 in downtwon and suburban areas. 8. The greatest proportion or 70.0 per cent of positive cases in X-ray examination was unregistered in August, 63.6 per cent in July 1973. 9. 220 out of 1,108 cases(19.9%) were unregistered pulmonary tuberculosis in X-ray and bacteriological examinations. 10. For age groups of unregistered caes, most prevalent age group was 30 to 39. 11. Regarding on the cases of unregistration, 'indifference for disease' occupied highest rate with 31.9 percent' and 'private secret' with 15.7 per cent. 12. Of the total 457 cases drop-outed, there were 78 complete heald cases while remaining 207 inactive.

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Optimum Feeding Rates for Growing and Sub-adult Olive Flounder Paralichthys olivaceus Fed Practical Extruded Pellets at High Water Temperature (고수온에서 사육한 성장기 및 미성어기 넙치(Paralichthys olivaceus)의 배합사료 적정 공급률)

  • Kim, Sung-Sam;Kim, Kang-Woong;Kim, Kyoung-Duck;Lee, Bong-Joo;Han, Hyon-Sob;Lee, Kyeong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.5
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    • pp.681-687
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    • 2015
  • We investigated the effects of feeding rate on the growth performance of growing and sub-adult olive flounder Paralichthys olivaceus, and the optimum feeding rate at high water temperature (25-31℃). In experiment I, two replicated groups of fish (113 g) were fed a commercial diet (CD) at rates of 0, 0.7, 1.2, 1.7, 2.2, and 2.4% (satiation) body weight (BW) per day for 4 weeks. In experiment II, two replicated groups of fish (313 g) were fed a CD feeding rates of 0, 0.3, 0.5, 0.7, 0.9, and 1.0% (satiation) BW per day for 4 weeks. In experiment I, the weight gain (WG) and specific growth rate (SGR) of fish fed at 0.7 and 0.9% BW per day was significantly higher than that of unfed fish (0%) and fish fed at 0.3, 0.5, and 1.0% (satiation) BW per day. In experiment II, the WG and SGR increased significantly as feeding rate increased from 0 to 2.2% BW per day, but decreased in the satiation group (2.4%). Broken-line regression analysis of WG showed that the optimum feeding rate of growing and sub-adult olive flounder were 1.98% and 0.55% BW per day, respectively, at the high water temperature.

Effects of Compost, Rate and Split Application of Nitrogen on Growth and Yield of Sweet Corn (퇴비시용과 질소시비 방법이 단옥수수의 생육 및 수량에 미치는 영향)

  • 강영길;박승의;박근용;문현귀;이성재
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.2
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    • pp.140-145
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    • 1985
  • Field experiments were conducted in Suwon in 1982 and 1983 to evaluate the effects of compost application, nitrogen (N) rate and split application of N on growth, yield and N uptake of sweet corn (Zea mays L.) grown under clear polyethylene mulching condition. Urea was banded at N rates of 0 (only in 1983), 5, 10, 15 and 20 kg per 10a with or without compost application of 1500 kg per 10a at planting. In 1982, half of N was sidedressed at the 5-6 leaf stage in case of 15, and 20 kg N per 10a. Compost application increased ear size and ear weight per 10a (10%). The number of ears per 10a markedly increased with an increase in N rates up to 10 kg per 10a but was not significantly affected by further increased N rates. Ear weight per 10a increased linearly as N rate increased from 0 to 20 kg per 10a without compost but increased greatly up to 10 kg N per 10a with compost application. Dry matter yield increased greatly up to 15, 10 kg N per 10a with and without compost application, respectively. Nitrogen uptake increased linearly from 8.1 to 18.8 kg per 10a with an increase in N rates from 0 to 20 kg per 10a. The optimum N rate appeared to be 10 to 15 kg per 10a based on ear number, ear weight and dry weight per 10a. There was no significant difference between basal and split applications of N in terms of ear size, and number and weight of ears per 10a.

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The growth and productivity of native Indonesian rice progenies and its relationship with root development during dry-season

  • Zakaria, Sabaruddin;Fitrya, Farid;Kurniawan, Trisda;Hereri, Agam Ihsan;Maulana, Teuku
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.338-338
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    • 2017
  • One of the problems in increasing rice production is getting lines or varieties that have high adaptability so that able to produce maximum production in a variety of environmental conditions. One strategy that can be done to get adaptive varieties is through the improvement of native varieties. This research was conducted in rain fed paddy field, Aceh province, Indonesia from June to September 2014. The texture of the soil was clay-loam with the soil pH ranged from 6.5-6.7. Five potential progenies of rice from crossing between native Indonesian rice with introduced rice varieties consist of C3, C4, S3, S5, S6 were used in this study. Besides that, one national rice variety Ciherang also used as a comparison. The plants were growth in the plot with the size of $2m{\times}1.4m$ with plant distance was $20cm{\times}20cm$. The fertilizers used in this study were Urea, NPK, and KCl. Randomized block design with 6 rice progenies/variety and 3 replications were used in this study. There were 18 experimental units and each experimental unit had 10 samples for the sources of data. The variables that were observed in this study including plant height at harvesting time, number of productive tiller, the percentage of empty grains and filled grain per panicle, weight of filled grains per hill, weight of filled grain per plot and yield potential per hectare. Analyzed were also conducted for the depth of root penetration, dry-root weight, dry-shoot weight, shoot-root weight ratio and its correlation with the weight of filled grain per hill. The research results show that there was significant difference on plant height at harvesting time, number of productive tillers, the percentage of empty grains and filled grain per panicle, weight of filled grains per hill, weight of filled grain per plot and yield potential per ha (p>0.01) among the treatments. In addition, depth root penetration, dry-root weight, dry-shoot weight, shoot-root weight ratio also had significant difference (p>0.01) among the treatment. The highest plant at harvesting time was found in S6, reaching 129.8 cm and the shortest plants was found in C3 reaching 107.5 cm. The largest number of productive tillers and the highest percentage of filled grains per panicle were found in Ciherang reaching 10.5 tillers and 80.7% respectively. Ciherang also had the heaviest weight of filled grains per hill and per plot reaching 21.1 g and 2.18 kg respectively. Whereas, S6 had the lowest number of tillers and the lowest percentage of filled grain per panicle.. The highest yield potential per ha was found in Ciherang reaching 7.79 tons. Among the progenies, S5 had the highest yield potential reaching 5 tons/Ha. The result also showed significant relationship between shoot-root weight ratio with weight of filled grains per hill. The highest value of shoot-root weight ratio (1.57) in Ciherang is thought had closed relationship with its yield potential.

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The Effect of the Regional Factors on the Variation of Suicide Rates: Geographic Information System Analysis Approach (Geographic Information System 분석방법을 활용한 시·군·구 지역별 자살률에 영향을 미치는 요인 분석)

  • Park, Seong-Yong;Lee, Kwang-Soo
    • Health Policy and Management
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    • v.24 no.2
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    • pp.143-152
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    • 2014
  • Background: Previous studies showed that the characteristics of population and regions were related to the suicide rates. This study purposed to analyze the relationships between regional factors and suicide rates with spatial analysis model. Methods: This is a cross sectional study based on the statistics of 2011 which was extracted from the 229 City Gun Gu administrative districts in Korea. Cause of death statistics on each district was used to produce the age-, sex-adjusted mortality rates resulting from suicide. Regional characteristics were measured by the number of doctors engaged in medical institutions per 1,000 population, divorced people's rate per 1,000 population, number of marriages per 1,000 population, and percent of welfare budget in general accounting. Statistical analysis was performed by using SAS ver. 9.3 and ArcGIS ver. 10.2 was used for geographically weighted regression (GWR). Results: In ordinary least square (OLS) regression, divorced people's rate per 1,000 population had a significant positive relationship with the standardized mortality rate per 100,000 population. Marriages per 1,000 population and the proportion of welfare budget in the general accounting had significant negative relationships with the mortality rates. Meanwhile, GWR provided that the directions of variable, divorced people's rate per 1,000 population, were varied depending on regions. The adjusted $R^2$ was improved from the 0.32 in OLS to the 0.46 in GWR. Conclusion: Results of GWR showed that regional factors had different effects on the suicide rates depending on locations. It suggested that policy interventions for reducing the suicide rate should consider the regional characteristics in obtaining policy objectives.

Feeding Frequency Influences the Growth, Food Consumption, Body Composition and Hematological Response of the Korean Rockfish Sebastes schlegelii (조피볼락(Sebastes schlegelii)의 성장, 사료 섭취, 체성분 및 혈액성상에 미치는 사료 공급 횟수의 영향)

  • Oh, Sung-Yong;Park, Jin Woo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.5
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    • pp.600-606
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    • 2016
  • The effects of feeding frequency on the growth, food consumption, body composition, and hematological response of the Korean rockfish Sebastes schlegelii were investigated for 77 days at ambient water temperatures (17.2-24.5℃) in a sea cage in Tongyeong, Korea. Three replicate groups of fish were hand-fed to satiation with a commercial diet in one of four different feeding frequency trials (one meal every 2 days, and one, two and three meals per day). At the end of the experiment, the mean weight gain, specific growth rate, and daily feed intake of fish fed one and two meals per day were significantly higher than those of fish fed one meal every 2 days or three meals per day. The feed efficiency of the fish fed three meals per day was significantly lower than that of the fish in the other groups. The glucose concentration of fish fed one meal every 2 days was significantly higher than that of the other groups. We conclude that the optimum feeding frequency for improving the growth of Korean rockfish weighing 100-200 g reared in sea cages is one meal per day under our experimental conditions.