• Title/Summary/Keyword: PED

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Atomic structure and crystallography of joints in SnO2 nanowire networks

  • Hrkac, Viktor;Wolff, Niklas;Duppel, Viola;Paulowicz, Ingo;Adelung, Rainer;Mishra, Yogendra Kumar;Kienle, Lorenz
    • Applied Microscopy
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    • v.49
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    • pp.1.1-1.10
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    • 2019
  • Joints of three-dimensional (3D) rutile-type (r) tin dioxide ($SnO_2$) nanowire networks, produced by the flame transport synthesis (FTS), are formed by coherent twin boundaries at $(101)^r$ serving for the interpenetration of the nanowires. Transmission electron microscopy (TEM) methods, i.e. high resolution and (precession) electron diffraction (PED), were utilized to collect information of the atomic interface structure along the edge-on zone axes $[010]^r$, $[111]^r$ and superposition directions $[001]^r$, $[101]^r$. A model of the twin boundary is generated by a supercell approach, serving as base for simulations of all given real and reciprocal space data as for the elaboration of three-dimensional, i.e. relrod and higher order Laue zones (HOLZ), contributions to the intensity distribution of PED patterns. Confirmed by the comparison of simulated and experimental findings, details of the structural distortion at the twin boundary can be demonstrated.

Porcine epidemic diarrhea viruses from Vietnam: isolation, characterization, and neutralizing activity

  • Van Tan Do;Quang Lam Truong;Hoai Thu Dao;Thi Lan Nguyen;Minna Shin;Kyeong Ryeol Shin;Tae-Wook Hahn
    • Korean Journal of Veterinary Research
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    • v.63 no.2
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    • pp.18.1-18.8
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    • 2023
  • Porcine epidemic diarrhea (PED) is characterized by acute enteritis, watery diarrhea, weight loss, dehydration, and death, with high mortality in neonatal piglets. In this study, 3 virus isolates collected in Vietnam between 2016 and 2017 were propagated successfully in Vero cells at high virus titers. Sequence analysis of the full-length spike (S) gene showed that all 3 isolates belong to genogroup 2b, which is closely related to other prevalent Asian strains. A comparison of the amino acid sequence revealed a 98.19% to 99.13% homology with the Vietnam isolates circulating during 2013-2015, suggesting that field PED viruses (PEDVs) are evolving continuously. Experiments in animals showed that the antisera from guinea pigs immunized with the vaccine strain resulted in higher levels (5 log2) of neutralizing antibodies against the homologous strain and a relatively moderate level of neutralizing antibodies against the field isolates. This finding would be helpful in selecting a PEDV strain for vaccine development.

Sequence analysis of spike genes of porcine epidemic diarrhea virus (PEDV) from Jeonbuk province (전북지역 돼지유행성설사 바이러스 Spike 유전자분석)

  • Mi Seon Kang;Woo Ri Jung;Seung Hyuck Yang;Keum Suk Chu
    • Korean Journal of Veterinary Service
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    • v.47 no.1
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    • pp.9-17
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    • 2024
  • Porcine epidemic diarrhea (PED) is a highly contagious enteric viral disease of pigs with watery diarrhea in piglets, which ultimately results in huge economic losses in the swine industry. The spike (S) protein plays an important role in viral pathogenicity, tissue tropism, infection, dissemination and the trypsin-dependent proliferation of the PED virus (PEDV). In the present study, we determined the full-length spike (S) gene sequences of twenty PEDV field strains detected in Jeonbuk province in 2022. Phylogenetic analysis showed that the twenty PEDV field strains were classified into G2b group and shared 98.6~100% of nucleotide homology and 97.4~100% of amino acid homology with each other. Mutations of amino acid sequences on the neutralizing epitope of S protein were observed in the twenty field strains compared to the previous vaccine strain SM-98-1 (G1a group). Therefore, these amino acid mutations in the PEDV S protein may result in a new genotype of the virus and highly pathogenic virus, so continuous monitoring is required.

Is Skeletonized Internal Mammary Artery Harvesting better than Pedicled Harvesting in Respect of the Sternal Blood Flow\ulcorner: An Estimation Using Bone Scan (내유동맥의 골격화 채취는 흉골로의 혈류 감소 측면에서 과연 유리한가 \ulcorner: 골주사를 이용한 평가)

  • 손국희;김영삼;김정택;윤용한;김광호;최원식;백완기
    • Journal of Chest Surgery
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    • v.37 no.6
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    • pp.511-516
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    • 2004
  • Background: One of the theoretical advantages of skeletonized internal mammary artery harvesting in coronary artery bypass surgery is to minimize the interruption of the sternal blood flow inevitably accompanied by internal mammary harvesting. A study using bone scan is designed to determine the effects of internal mammary artery harvesting technique on the sternal blood flow. Material and Method: From April 2002 to March 2003, 27 patients out of 48 patients who underwent the isolated coronary bypass surgery were enrolled into the study. The enrolled patients underwent bone scan in the preoperative period and postoperative period respectively. Bilateral internal mammary artery was used in 8 patients (BIMA group) and single left internal mammary artery in 19 patients (LIMA group). The patients in LIMA group were divided into two groups: LlMA_skel group, in whom left internal mammary artery was harvested in skeletonized fashion (n=12), and LlMA_ped group, in whom left internal mammary artery was harvested in pedicled fashion (n=7). After the bone scan, the region of interest (ROI) was created on the left of the sternum and the mirror image with the same pixel numbers was placed on the right half of the sternum. The mean counts per pixel on the left side of the sternum was compared with those on the right side and expressed as left to right ratio (L/R ratio). Result: In LIMA group, the L/R ratio decreased from 94.6$\pm$4.1% to 87.9$\pm$6.9% (p=0.003) after the operation as compared to BIMA group, in which no change of the L/R ratio was observed. The changed of the L/R ratio in LlMA_skel group and LlMA_ped group were from 95.3$\pm$4.2% to 88.3$\pm$7.7% and from 93.4$\pm$3.9% to 87.4$\pm$5.8% respectively. The % changes in L/R ratio were -7.44 $\pm$7.08 in LIMA_skel group and -6.17$\pm$9.08 in LiMA_ped group, which did not reach the statistical difference. Conclusion: Ipsilateral sternal blood flow is interrupted by internal mammary artery harvesting as evidenced by the decrease in L/R ratio after left internal mammary artery harvesting irrespective of the harvesting techniques. Skeletonized harvesting did not show superiority in respect to sternal blood flow as compared to pedicled harvesting.

A Survey on the Envlronmcntal Sanitary Status of Water Supply System in Rural Area (농촌급수시설에 관한 환경위생확적 조사연구)

  • 박국환;김성자
    • Journal of Environmental Health Sciences
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    • v.5 no.1
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    • pp.76-85
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    • 1978
  • This survey was undertaken for the period seven month beginning January 15, 1977 and ending July 31, 1977 to detect the general sanitary status of the villages and the villagers and, at the same time, analyse quality of water sources with emphasis on a total of 1,256 households dividing into three different groups: such as, 280 households were selected as random samples from the area of the sophisticated piped water supply system, 122 households from the area of the simplified water supply system and finally 854 households from the area of nonpi-ped water supply system. The following results were concluded after quality of water sources had been analysed and conditions of the environmental sanitation had been reviewed: 1. 11.2% of the respondents from the area of the sophisticated piped water supply system responded that quantity of drinking water lacked to meet their demand while 30.6% of the villagers from the area of nonpi-ped water supply system responded quantity of drinking water didn't meet their demand. 2. 30.8% of the.respondents from the area of the sophisticated water supply system responded that contaminating source located within 15 meters from the water source while 54.4% of the respondents from the non-piped water supply system claimed the same. 3. It was found that water from all sampling areas were positive in coliform group with exception of Moonsan which is one of the sophisticated piped water supply system groups and the number of general bacteria exceeded the government standard criteria of water quality in the area of the nonpi-ped water supply system. 4. In relation with time requirement to draw water in the area of non-piped water supply system, 76 respondents claimed it requires less than 15 minutes to draw water, 15.0% claimed 15 to 30 minutes and 9.0% claimed more than 30 minutes. 5. In relation with knowledge on sanitation of drinking water, 30.8% of respondents from the area of the sophisticated piped water supply system and 41.8% of respondents from the area of nonpiped water supply system denied possible existence of germ in drinking water they drink, while 17.4% of the respondents from the area of the sophisticated water supply system and 50.2% of non-peped water supply system thought it safe to drink water without any treatment. 6. 60.0% of the respondents from the area of non-piped water supply system and many of them believed that their health status will be improved by installation of a sophisticated water supply system in their area. 7. The respondents from the areas of piped water supply sytem expressed greater concern over drinking water sanitation than those from the areas of non-piped water supply system and sanitary conditions were found the same. It was, therefore, proved that knowledge of environmnntal sanitation contributed a great deal to improve sanitary conditions of the villages and villagers and at the same time health education, especially environmental sanitation, will be played a important role to improve their sanitary conditions.

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Genetic sequence and phylogenetic analysis of spike genes of porcine epidemic diarrhea virus (PEDV) in Jeonbuk province (전북지역 돼지유행성설사 바이러스 Spike 유전자 염기서열 및 계통분석)

  • Park, Mi-Yeon;Moon, Bo-Mi;Gang, Su-Jin;Lee, Jong-Ha;Park, Jin-Woo;Cho, Sung-Woo;Her, Cheol-Ho
    • Korean Journal of Veterinary Service
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    • v.44 no.2
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    • pp.73-83
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    • 2021
  • Although many swine farms continuously vaccinated to sow to prevent Porcine epidemic diarrhea(PED), PED has occurred annually in swine herds in Jeonbuk province, Korea. In the present study, the small intestine and feces samples from 17 farms where severe watery diarrhea and death of newborn piglets occurred in 2019 were collected, amplified by RT-PCR and determined the complete nucleotide sequences of the spike (S) glycoprotein genes of nine Jeonbuk PEDV isolates. The spike (S) glycoprotein is an important determinant for molecular characterization and genetic relationship of PEDV. These nine complete S gene isolates were compared with other PEDV reference strains to identify the molecular diversity, phylogenetic relationships and antigenicity analysis. 9 field strains share 98.5~100% homologies with each other at the nucleotide sequence level and 97.3~100% homologies with each other at the amino acid level. The nine Jeonbuk PEDV isolates were classified into G2b group including a genetic specific signal, S-indels (insertion and deletion of S gene). In addition, comparisons the neutralizing epitopes of S gene between 9 field strains and domestic vaccine strains of Korea mutated 12-15 amino acids with SM-98-1 (G1a group) and mutated 0-3 amino acids with QIAP1401 (G2b group). Therefore, the development of G2b-based live vaccines will have to be expedited to ensure effective prevention of endemic PED in Korea. In addition, we will need to be prepared with periodic updates of preventive vaccines based on the PEDV variants for the re-emergence of a virulent strain.

농장탐방-도암농장

  • Bae, Seung-Han
    • The Korea Swine Journal
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    • v.28 no.9 s.325
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    • pp.204-207
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    • 2006
  • 경기도 이천에 위치하고 있는 모든 250두의 도암농장은 2003년 2~3월 사이 pmws로 40~45일령 구간의 자돈 300여 마리가 폐사했다. 도암농장을 경영하고 있는 이명규 사장은 전문가의 도움을 받아 모돈 및 자돈의 백신관리와 사육환경의 변화로 이를 극복했고 지금까지 PSY(piglet per sow per year)26.1두,MSY(Market per sow per year)25.6두의 성적을 내고 있다. 이는 모돈1두당 연간 이유 후 출하까지 폐사가 0.5두이며, 현재 전국MSY가 15~16두임을 감안한다면 도암농장의 성적은 기적 같다고 말할 수 있다. 또한, 전국적으로 PED가 극성을 부릴 때, 이 질병에 대한 피해를 거의 보지 않은 도암농장의 경험과 사양관리방법을 이번 월간양돈에 소개하고자 한다.

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4-Axis Decentralized Control of Magnetic Bearing Equipped whth Collocatd Capacitance Sensor (동위형 축전 센서가 장착된 자기베어링의 4 축 분산식 제어)

  • 신동원
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.04a
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    • pp.336-340
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    • 1996
  • This paper presents the development of a collocated capacitance sensor and its application to the decentralized PID controller design for 4-axis magnetic bearing system. The main feature of the sensor is that it is made of a compact printed circuit board (PCB) so that it can be built in to the actuator coil of the magnetic bearing unit. The signal processing unit has been also developed. Then, decentralized PED controller is designed using simplified rotor system model. Finally, the experimental results on the performance of the collocated sensor based decentralized PID controller for a magnetic bearing rotor system is presented.

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Effects of gibberellin on alpha-and beta-amylase activities of Aspergillus oryzae (Aspergillus oryzae 의 alpha 및 beta-amylase 활성에 미치는 gibberellin 의 영향에 관한 연구)

  • 정기택;유대식
    • Korean Journal of Microbiology
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    • v.6 no.2
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    • pp.68-74
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    • 1968
  • Effects of gibberellin on alpha and beta-amylase activities of Aspergillus orygae var. microsporus have been studied. Results obtained are as follows: 1. The growth of mycelium and dry weight of surface ped was accelerated by 0, 0001% gibberellin solution, spores of Aspergillus oryzae var. microsporus. were preveously soaked for three days. 2. Adding to culture media with 0, 0015% gibberellin, alpha-amylase was increased 50% much as beta-Amylase was as much as 50%.

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