• Journal of Sensor Science and Technology
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• v.8 no.4
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• pp.327-332
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• 1999

Catalytic combustion type gas sensors were fabricated by using noble metal(Pt and Pd) added ${\gamma}-Al_2O_3$ powder with specific surface area of $200\;m^2/g$. The fabricated sensor showed power consumption of 500 mW at the operating voltage of 1.75 V and high sensitivity of about 120 mV for butane, methane, or propane 100%LEL, respectively. The sensor properties also showed good linearity to hydrocarbon gas concentration variation, reproductivity and stability for relative humidity variation. And it showed high stability in butane ambient for 100 days.

• International Journal of Oral Biology
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• v.35 no.3
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• pp.75-81
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• 2010

The present study investigated the role of ERK in the onset of mechanical and cold allodynia in a rat model of compression of the trigeminal ganglion by examining changes in the air-puff thresholds and number of scratches following the intracisternal injection of PD98059, a MEK inhibitor. Male Sprague Dawley rats weighing between 250 and 260 g were used. Under anesthesia, the rats were mounted onto a stereotaxic frame and received 4% agar ($10\;{\mu}l$) solution to compress the trigeminal ganglion. In the control group, the animals were given a sham operation without the application of agar. Changes in behavior were examined at 3 days before and at 3, 7, 10, 14, 17, 21, 24, 30, and 40 days after surgery. Compression of the trigeminal ganglion significantly decreased the air-puff thresholds. Mechanical allodynia was established within 3 days and persisted over postoperative day 24. To evaluate cold allodynia, nociceptive scratching behavior was monitored after acetone application on the vibrissa pad of the rats. Compression of the trigeminal ganglion was found to produce significant cold allodynia, which persisted for more than 40 days after surgery. On postoperative day 14, the intracisternal administration of $1\;{\mu}g$ or $10\;{\mu}g$ of PD98059 in the rat model significantly decreased the air-puff thresholds on both the ipsilateral and contralateral side. The intracisternal administration of $10\;{\mu}g$ of PD98059 also significantly alleviated the cold allodynia, compared with the vehicle-treated group. These results suggest that central ERK plays an important role in the development of mechanical and cold allodynia in rats with compression of the trigeminal ganglion and that a targeted blockade of this pathway is a potential future treatment strategy for trigeminal neuralgia-like nociception.

• The Journal of Korean Physical Therapy
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• v.31 no.2
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• pp.122-128
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• 2019

Purpose: This study examined the effects of sagittal spinopelvic alignment on the clinical parameters, motor symptoms, and respiratory function in patients with mild to moderate Parkinson's disease (PD). Methods: This study was a prospective assessment of treated patients (n=28, Hoehn and Yahr (H&Y) stage 2-3) in a PD center. Twenty-eight subjects ($68.5{\pm}5.7yrs$) participated in this study. The clinical and demographic parameters, including age, sex, symptoms duration, treatment duration, and H&Y stage, were collected. Kinematic analysis was conducted in the upright standing posture with a motion capture system. A pulmonary function test (PFT) was performed in the sitting position using a spirometer. The motor symptoms were assessed on part III of the movement disorder society sponsored version of the unified Parkinson's disease rating scale (MDS-UPDRS). SPSS 18.0 was used to analyze the collected data. Results: The exceeding 12 degrees group of the lower trunk showed significantly higher on the clinical parameters than the below 12 degrees group. In addition, the exceeding 12 degrees group of the lower trunk showed a significantly lower forced expiratory volume at one second (FEV1) / forced vital capacity (FVC) (%) and 25-75% forced mid-expiratory flow (FEF) (L/s) than in the below group. On the other hand, there was no difference in the upper trunk and the cervical pelvis between the groups. Conclusion: These findings suggest that the sagittal balance in the lower trunk is related to the clinical parameters and respiratory function, but not the motor symptoms in patients with mild to moderate PD.

• Resources Recycling
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• v.13 no.5
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• pp.28-36
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• 2004

The extraction of platinum group metals such as Pt, Pd and Rh from spent automobile catalyst has been investigated by leaching in HCl solutions using $HNO_3$ or NaOCl as a oxidant. The effect of type and amount of oxidant, reaction time and pulp density on the extraction of platinum group metals was examined. Platinum group metals were recovered by the cementation method using aluminum as a reducing agent. The extraction ratio was higher when NaOCl was used as a oxidant. The optimum leaching conditions were obtained to be: HCl 8 M, the amount of NaOCl 1.4 mole, leaching temperature $90^{\circ}C$, leaching time 180 minutes, pulp density 400g/L. Under the optimum conditions, the extraction of Pt, Pd and Rh were 96.1%, 93.6% and 77.3%, respectively. With the addition of 2.0g of aluminum which corresponds to 28 equivalent the reduction were 98% for Pt. 98.8% for Pd and 65.3% for Rh, respectively.

• Korean Journal of Pharmacognosy
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• v.44 no.4
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• pp.379-383
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• 2013

Portulaca oleracea L. is known to have many biological benefits such as anti-oxidant, anti-inflammatory, anti-allergic and anti-tumor. The objective of this study is to explore the neuroprotective effect of P. oleracea L. against glutamate-induced oxidative stress in mouse hippocampal HT22 cells. P. oleracea L. 70% ethanol extract and solvent fractions have the potent neroprotective effects on glutamate-induced nerotoxicity by induced the expression of heme oxygenase (HO)-1 in HT22 cells. Especially, ethyl acetate fraction showed higher protective effect. In HT22 cell, P. oleracea L. treatment with ERK inhibitor (PD98059) and c-JUN N-terminal kinase (JNK) inhibitor (SP600125) reduced P. oleracea L. ethyl acetate fraction induced HO-1 expression and P. oleracea L. ethyl acetate fraction also increased ERK and JNK phosphorylation. Furthermore, we found that treatment of P. oleracea L. caused the nuclear accumulation of Nrf2. In conclusion, the ethyl acetate fraction of 70% ethanol extract of P. oleracea L. significantly protect glutamate-induced oxidative damage by induction of HO-1 via Nrf2, ERK and JNK pathway in mouse hippocampal HT22. Taken together these finding suggest that P. oleracea L. ethyl acetate fraction is good source for taking active compounds and may be a potential therapeutic agent for brain disorder that induced by oxidative stress and neuronal damage.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.22 no.2
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• pp.58-62
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• 2022

Pompe disease (PD) is an autosomal recessive genetic disorder caused by a deficiency of the lysosomal enzyme acid α-glucosidase (GAA). It is easy to hastily diagnose as patients if they have two pathogenic variants. Clinical pathologists misdiagnosed our infant and her mother as PD. Here, we report a case of pseudodeficiency in a potential late-onset Pompe disease (LOPD) carrier with a double dual variant, each in cis formation in a 3-month infant. The person who has two pathogenic variants was diagnosed as a carrier, not a patient. It was first reported in Korea. The patient had: two likely pathogenic heterozygous mutations on exon #4: c.752C>T (p.Ser251Leu), c.761C>T (p.Ser254Leu), and a heterozygous mutation on exon #12: c.1726G>A (p.Gly576Ser), also with a heterozygous mutation on exon #15: c.2065G>A (p.Glu689Lys). By presenting this case we emphasize the possibility of cis formation of genes which may cause pseudodeficiency, and potential LOPD carrier form. Hereby we suggest that thorough evaluation of GAA gene is essential among whom initially diagnosed as PD.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.11
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• pp.5709-5714
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• 2012

Objective: To evaluate the effects of curcumin on matrixmetalloproteinase-9 (MMP-9) and invasion ability induced by transforming growth factor-${\beta}1$ (TGF-${\beta}1$) in MDA-MB-231 cells and potential mechanisms. Methods: Human breast cancer MDA-MB-231 cells were used with the CCK-8 assay to measure the cytotoxicity of curcumin. After treatment with 10 ng/ml TGF-${\beta}1$, with or without curcumin (${\leq}10{\mu}M$), cell invasion was checked by transwell chamber. The effects of curcumin on TGF-${\beta}1$-stimulated MMP-9 and phosphorylation of Smad2, extracellular-regulated kinase (ERK), and p38 mitogen activated protein kinases (p38MAPK) were examined by Western blotting. Supernatant liquid were collected to analyze the activity of MMP-9 via zymography. Following treatment with PD98059, a specific inhibitor of ERK, and SB203580, a specific inhibitor of p38MAPK, Western blotting and zymography were employed to examine MMP-9 expression and activity, respectively. Results: Low dose curcumin (${\leq}10{\mu}M$) did not show any obvious toxicity to the cells, while $0{\sim}10{\mu}mol/L$ caused a concentration-dependent reduction in cell invasion provoked by TGF-${\beta}1$. Curcumin also markedly inhibited TGF-${\beta}1$-regulated MMP-9 and activation of Smad2, ERK1/2 and p38 in a dose- and time-dependent manner. Additionally, PD98059, but not SB203580, showed a similar pattern of inhibition of MMP-9 expression. Conclusion: Curcumin inhibited TGF-${\beta}1$-stimulated MMP-9 and the invasive phenotype in MDA-MB-231 cells, possibly associated with TGF-${\beta}$/Smad and TGF-${\beta}$/ERK signaling.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.2249-2253
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• 2008

본 연구에서는 지점빈도분석과 지역빈도분석을 이용하여 확률홍수량을 산정 하였다. 지점빈도 분석은 Annual Maximum Series(AMS) 및 Partial Duration Series(PDS)를 이용하여 자료를 추출하고 각 자료에 적합한 확률분포를 이용하여 확률홍수량을 산정하였다. 그러나 AMS를 이용한 확률홍수량의 산정은 표본의 개수가 부족하면 이에 따른 변동성(variability)이 커지게 되는 단점이 존재하며, PDS를 사용하면 임계값(threshold)에 따른 주관적 영향이 결과에 반영되는 단점이 존재하는 것으로 알려져 있다. 따라서 본 연구에서는 PDS를 사용하는 경우의 단점을 해결하기 위해 연 1.7회의 발생횟수를 갖는 자료를 추출하고 몬테카를로 모의시험을 통하여 주관적 영향을 제거하였다. 또한 두 가지 방법에 의해 산정된 확률홍수량의 비교검토를 위해 지역빈도분석을 수행하였다. 유역의 면적과 일평균강우량으로부터 확률홍수량을 산정할 수 있는 것으로 알려진 Bayesian-Generalized Least Square(B-GLS) 방법을 이용하여 확률홍수량을 산정하였다. 최종적으로 안양천 유역의 13개 소유역에 대한 세 가지 방법에 의해 산정된 확률홍수량을 비교 검토한 결과, 특정한 방법이 항상 우수하다는 결론은 얻을 수 없었으나 각 유역별로 AMS가 가장 크고 B-GLS가 가장 작은 확률홍수량을 갖는 경향을 나타내었다.

• Journal of the Korean Chemical Society
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• v.40 no.7
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• pp.492-500
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• 1996

The solvent extraction of trace Bi, Cd and In in seawater samples using ammonium pyrrolidine dithiocarbamate(APDC) as a complexing agent was studied. The pH of sample solution, the amount of APDC, the type of solvent and the shaking time were investigated together with back-extraction conditions. After the pH of 200 mL seawater was adjusted to 4.0 and 5.0 mL of 1% APDC was added, analytes were extracted with 10.0 mL of MIBK by shaking for 35 minutes. The organic phase seperated was washed with a 0.05 M NaOH 10.0 mL to remove HPDC. The analytes were stripped by the back-extraction of 5 minute shaking with 5 mL of 4 M HNO3 containing 150 ㎍/mL Pd(Ⅱ). Detection limits of Bi, Cd and In were 0.038, 0.0057 and 0.023 ng/mL, respectively. Both of Bi(Ⅲ) and In(Ⅲ) were not detected in two kinds of water samples of the East Sea and the contents of Cd(Ⅱ) were 0.018 and 0.016 ng/mL. The recoveries of over 90% showed that this procedure was applicable to the determination of such trace elements in seawater samples.

• KSBB Journal
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• v.20 no.1 s.90
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• pp.55-59
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• 2005

Toxicity test for biodiesel (BD), biodiesel-derived neopentyl polyol ester (NPE) lubricant oil base, lubricant oil for diesel engine (LODE) and petroleum diesel (PD) was carried out using microalgae, Chlorella vulgaris. According to the method of OECD 201, the $EC_{50}$ values of BD and NPE were estimated as 84 and 69 mg/L, respectively, which indicates that these compounds are classified as slightly toxic compounds. The $EC_{50}$ values of LODE and PD were measured as 42 and 24 mg/mL, respectively, showing that these compounds are considered as moderately toxic compounds.