• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.139.1-139.1
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• 2003

The effects of liriodenine, an aporphine isoquinoline alkaloid, on dopamine biosynthesis and L-DOPA-induced neurotoxicity in PC12 cells were investigated. Treatment of PC12 cells with liriodenine at 10 $\mu\textrm{M}$ showed 33.6％ inhibition of dopamine content decreased at 3 h and reached a minimal level at 12 h after the exposure to liriodenine at 10 $\mu\textrm{M}$. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.254.1-254.1
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• 2002

The effects of aporplline isoquinoline alkaloids such as liriodenine. anonaine and asimilobine on dopamine biosynthesis in PC12 cells were investigated. Treatment of PC12 cells with liriodenine (10 ${\mu}$M), anonaine (0.05 ${\mu}$M) and asimilobine (0.15 ${\mu}$M) showed 33.6%, 37.7% and 35.1 % inhibition of dopamine content for 12 h. The IC$\sub$50/ values of liriodenine. anonaine and asimilobine were 8.4 ${\mu}$M. 0.05 ${\mu}$M and 0.13 ${\mu}$M. respectively. (omitted)

• 대한한의학회지
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• 제28권2호통권70호
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• pp.213-223
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• 2007

Objective : Alzheimer's disease (AD) is a geriatric dementia that is widespread in old age. With an aging populace, AD is a looming problem in public health service. Alzheimer's disease is characterized by specific neuronal degeneration in certain areas of the brain. Mutations and abnormal expression of several genes are associated with ${\beta}-amyloid$ deposits and Alzheimer's disease; among them APP, PS1, and PS2, SOD, free radical, ROS. Methods:We studied herbal medicines that have a relationship to brain degeneration. From pre-modern times, although a variety of oriental prescriptions of Aster tataricus have been traditionally utilized for the treatment of AD, their pharmacological effects and action mechanisms have not yet been fully elucidated. Result : Based on morphological observations by phase-contrast microscope, TUNEL assay and MTT in the culture media, $H_20_2-induced$ cell death was significantly inhibited by Aticus. We examined by ROS formation, catalase activity and GSH activity. We studied the protective effect and inhibitory effects of neurotoxicity in $H_20_2-induced$ PC-12 cells by Aticus. Findings from our experiments show that Aticus inhibits apoptosis, which has neurotoxicities and cell damage in PC-12 cells. In addition, treatment with Aticus ($>25{\mu}g/ml$ for 6hrs) partially prevented $H_20_2-induced$ cytotoxicity in PC-12 cells, and induced a protective effect. Conclusion : As the result of this study, in the Aticus group, the apoptosis in the nervous system was inhibited, protected against the degeneration of PC-12 cells by $H_20_2$. Taken together, Aticus exhibited inhibition of $H_20_2-induced$ apoptotic cell death. Aticus was found to induce protective effect on GSH and catalase in PC-12 cells. Based on these findings, Aticus may be beneficial for the treatment of AD.

• 생명과학회지
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• 제23권8호
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• pp.1041-1049
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• 2013

원지의 뿌리는 기억력을 향상시키는 동양의 전통 약재로 널리 알려져 있다. 그러나 그 작용기전은 아직까지 해명되지 않았다. 본 연구에서는 항산화 효과 뿐만 아니라 신경 세포 에서 파생된 PC12 세포의 acetylcholinesterase(AchE) 활성과 연관된 인지능에 대한 원지열수추출물(PRHWE)의 효과를 in vitro 및 살아 있는 세포에서 조사하였다. 먼저 MTT assay을 이용한 세포생존에 대한 연구에서 PRHWE는 0.1% 이하의 농도에서 세포독성이 없는 것으로 나타났다. PRHWE는 DPPH radical, hydrogen peroxide 및 superoxide의 소거활성과 환원력이 농도에 비례하여 증가시킨다는 것이 관찰되었다. 특히 PRHWE는 hydroxyl radical에 의하여 유발되는 DNA의 산화에 대한 보호효과를 나타내었다. 부가적으로 그것은 신경성세포에서 nitric oxide의 생성을 억제하였다. 더욱이 AchE 활성은 PRHWE의 농도에 비례하여 감소하였다. 뿐만 아니라, PRHWE는 PC12 세포에서 SOD-1과 NOS-2 발현수준을 증가 시킨다는 것이 발견되었다. 더욱이 reporter gene assay를 이용한 실험에서 p53과 NF-${\kappa}B$의 전사활성이 PRHWE의 존재 하에서 감소했다. 그러므로 이러한 결과들은 PRHWE가 신경성세포에서 항산화 활성 및 신경 세포 보호 효과가 있다는 것을 증명하였고, 이는 PRHWE가 사람의 건강을 위한 치료제로 큰 잠재성을 가지고 있다는 것을 시사한다.

• Applied Biological Chemistry
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• 제50권1호
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• pp.63-67
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• 2007

본 연구에서는 석곡(Dendrobium nobile Lindl.) 추출물에 대한 신경세포 보호 효과를 확인하기 위하여 $H_{2}O_{2}$에 의해 유도된 스트레스 상태의 PC12 세포주에서 MTT-dye reduction assay와 LDH release assay를 이용하였다. MTT reduction assay 결과, 스트레스 상태에서는 47%의 생존율을 보인데 반하여 석곡 추출물 50${\mu}$g/ml 농도로 처리하였을 때 99.5%의 높은 세포 생존율을 확인할 수 있었다. 이 결과는 LDH release assay 에서도 일치하는 결과를 확인하였다. 그리고 광학 현미경을 이용한 형태학적 변화를 관찰한 결과에서도 신경돌기의 출현 유도를 통한 신경세포 생존을 확인 할 수 있었다. 또한 핵의 변화에 미치는 영향을 관찰한 결과 정상 세포의 핵은 타원형의 온전한 핵 모양을 나타낸 반면 산화적 손상을 입은 세포는 apoptotic body가 핵 주변에 나타나는 전형적인 apoptosis를 나타내었고 석곡 추출물을 처리한 결과 핵의 condersation현상과 fragmentation이 현저히 감소함을 확인 할 수 있었다. 그리고 $H_{2}O_{2}$에 의한 capase-3의 활성은 석곡 추출물을 50${\mu}$g/ml 처리 후 약 1.1배 이하로 감소함을 보였다.

• Natural Product Sciences
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• 제10권3호
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• pp.104-108
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• 2004

Five lignans including gomisin N (1), wuweizisu C (2), gomisin L1 (3), (+)-deoxyschizandrin (4), and gomisin J (5) have been isolated from the fruits of Schizandra chinensis. The structures of the isolated compounds were elucidated by analyzing MS and NMR spectra. Effects of the compounds isolated in this study on the dopamine content in PC12 cells were investigated to evaluate their inhibitory effectiveness. The gomisin N, wuweizisu C, and gomisin J showed 25.4%, 39.8%, and 35.1 %, respectively, inhibition effect on dopamine content in PC12 cells at the concentration of $50\;{\mu}g/ml$.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2005년도 춘계학술대회 논문집
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• pp.3-8
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• 2005

We introduced mechanical stimuli and micropatterned substrate with micro fibers to investigate the effects of those on neurite outgrowth along with nerve growth factor (NGF) in vitro. Microfiber substrates were fabricated using an electrospinning process. And PC-12 cells cultured on substrates were simulated with nerver growth factor and laminar flow shear stress in a fluid flow system The results suggest that microfiber substrates and fluid-induced shear stress are promising for simulating neuronal regeneration in a desired direction.

• Archives of Pharmacal Research
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• 제29권11호
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• pp.963-968
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• 2006

Seven phenolic compounds (1-7) were isolated from the tubers of Gastrodia elata. Their structures were elucidated on the basis of MS and NMR spectral data. p-Ethoxymethyl phenyl-O-${\beta}$-D-glucoside (1) was proved to be a new compound, with N-(p-hydroxybenzyl)-adenosine (7) isolated from this plant for the first time. In this study, the protective effects of the six constituents (1-6) on PC12 cells against the cytotoxicity induced by KCI and glutamate were also investigated. The viability of the PC12 cells was significantly enhanced by pretreatment with the six phenolic constituents.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1997년도 학술발표회
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• pp.32-32
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• 1997

The regulatory role of A$\sub$2A/ adenosine receptors in P$_2$ purinoceptor-mediated calcium signaling was investigated in rat pheochromocytoma (PC12) cells. When PC12 cells were treated with 2-p-(2-carboxyethyl)phenethylamino-5'-N-ethylcarboxamidoadenosine (CGS21680), a specific agonist of the A$\sub$2A/ adenosine receptor, extracellular ATP-evoked [(CA$\^$2+/)]$\sub$i/ rise was inhibited by 20%.(omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.79.1-79.1
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• 2003

Aster scaber T. (Asteraceae) has been used in traditional Korean and Chinese medicine to treat bruises, snakebites, headaches and dizziness. (-) 3,5-Dicaffeoyl-muco-quinic acid (DQ) isolated from Aster scaber induced neurite outgrowth in PC12 cells. It has been reported that the activation of the extracellular signal regulated kinase1/2 (Erk 1/2) and phosphoinositide 3 (P13) kinase plays a crucial role in the NGF-induced differentiation of PC12 cells. This study showed that the effect of DQ on neurite outgrowth is mediated via the Erk 1/2 and PI3 kinase-dependent pathways like NGF. (omitted)