• Title/Summary/Keyword: PBMC와 비장세포의 증식

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사료 중 크릴 분말이 살모넬라 LPS로 자극한 브로일러의 비장세포와 PBMC 증식에 미치는 영향

  • 임진택;박인경;김재환;고태송
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2002.11a
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    • pp.94-95
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    • 2002
  • In order to evaluate the effect of dietary krill meal on immunity of broilers, the proliferation of splenocyte and PBMC (pheripheral blood mononuclear cell) from broilers fed experimental diets containing 0.0, 0.5, 1.0 and 2.0 o/o krill meal, respectively, and injected the Salmonella typhymurium lipopolysaccharide (LPS) were assayed. The proliferation of splenocyte was increased with the dietary krill levels, but was decreased with the LPS immunlogical stress. Con A addition in the medium increased the proliferation of the splenocytes from birds fed dietary krill or stimulated by LPS. In 21 day old broilers, dietary krill meal and addition of Con A decreased the proliferation of PBMC while enhanced proliferation of PBMC was shown in birds stressed by the LPS during 2nd week of age. The results indicated dietary krill meal affected immune response in broiler.

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Effect of Dietary Krill Meal Levels on the Cell Mediated Immunity in Intra-muscularly Croton Oil Injected Broiler Chicks (파두유를 주입한 육계병아리에서 사료중 크릴밀 수준이 세포성 면역에 미치는 영향)

  • Im, J.T.;Park, I.K.;Koh, T.S.
    • Journal of Animal Science and Technology
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    • v.49 no.5
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    • pp.599-610
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    • 2007
  • Effect of dietary krill meal levels on the cellular immunity was studied in broiler chicks activated immune response. One day old male broiler chicks(Ross) were fed the experimental krill meal 0.0(basal), 0.5, 1.0 and 2.0% diets for 3wks. Blood TNF-α activity, ovotransferrin level and Con A induced proliferation of PBMC and splenocytes after 24 hr(21 d age) of the croton oil 10㎕ injection intra- musculary at the age of 20 days compared to the control olive oil. Krill meal diets did not affect growth performance of broiler chicks and plasma ovotransferrin levels but decreased significantly(p<0.0001) TNF-α like activity and proliferation of PBMC relative to krill meal 0.0% diet. And the proliferation of splenocytes were significantly(p<0.05) increased in birds fed krill meal 1.0% diet relative to krill meal 0.5 and 2.0% diets. The croton oil injection induced a significant(p<0.0001) increases in the TNF-α activity or the PBMC proliferation and enhanced circulating ovotransferrin levels relative to the olive oil. In birds injected with the croton oil the proliferation of PBMC was reduced linearly with the increase of dietary krill meal levels, and the proliferation of splenocytes was decreased in the krill meal 1.0 and 2.0% diets relative to olive oil. These results indicated that dietary krill meal changed the innate and cellular immunity in broiler chicks activated by the injection of croton oil.

Proliferation Assay of Splenocyte and PBMC by the Evaluation of Alamar Blue Dye Reduction Value in Broiler Chicks (Alamar Blue 색소의 환원량 평가에 의한 급성기 반응중 육계병아리의 비장세포와 PBMC 증식도 측정)

  • Im, J.T.;Park, I.K.;Koh, T.S.
    • Journal of Animal Science and Technology
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    • v.49 no.2
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    • pp.213-224
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    • 2007
  • In this study, hatched male broiler chicks(Ross) were fed on a basal diet and LPS was administered via intraperitoneal injection three times every other day, on the 9th, 11th and 13th days of the experiment, and then PBMC and splenocytes were isolated on day 14. The degree of alama blue reduction was evaluated at 4, 24, 48, 96 and 120 h in the splenocytes, and at 4, 8, 12, 24 and 48 h for PBMC of incubation after the addition of alama blue solution to the media. The cell numbers used in this experiment were 103, 104 and 105 cells per well, and the con A levels were 0.0, 1.0, 5.0, and 10.0 ㎍ per ml of medium. 1. The degree of alama blue reduction was found to increase in a linear fashion with increasing incubation time and cell numbers, for both splenocytes and PBMC. 2. During acute phase response, the degree to which alama blue was reduced was significantly elevated (p<0.05) at an incubation time of 24 hr for the splenocytes, 4 hr for PBMC, and a cell number of 105 cells per well, respectively. 3. The raised reduction of alama blue to control was linear with Con A levels in medium, and higher reduction in Con A 10.0 ㎍ relative to 1.0 or 5.0 ㎍ in ml medium was shown 4. The medium with autologous serum evidenced a significantly (p<0.05) higher reduction of alama blue relative to FBS. 5. Splenocytes and PBMC from the LPS-injected birds evidenced significantly higher levels of alama blue reduction regardless of incubation time, number of cells, level of Con A added, or serum type, as compared with what was observed in normal birds. The results indicated that the assay conditions for proliferative activity using the alama blue method in birds in which the acute phase response had been activated via intraperitoneal LPS injection requires 4 hrs of incubation for PBMC, 24 hrs of incubation for splenocytes, and 10㎍ of Con A per ml of medium.

Effect of Dietary Krill Meal Levels on Performance and Immune Response of Broiler Chicks Injected with Salmonella typhimurium Lipopolysaccharide (살모넬라 LPS를 주입한 육계병아리에 있어서 사료 중 크릴 밀 수준이 생산성과 면역반응에 미치는 영향)

  • Im, J.T.;Park, I.K.;Koh, T.S.
    • Journal of Animal Science and Technology
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    • v.49 no.2
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    • pp.225-238
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    • 2007
  • In this study, the effects of dietary krill meal levels on cellular immunity in LPS-injected broiler chicks was evaluated. One day-old male broiler chicks(Ross) were fed on the experimental basal meal(0.0% krill meal), or diets containing 0.5%, 1.0% and 2.0% krill for 3 weeks, and the acute phase response was activated by intraperitoneally injection of Salmonella typhimurium lipopolysaccharide(LPS) 3 times at 9, 11, and 13 days of age. 1. Acute phase response induced a significant reduction in(p<0.05) daily weight gain and feed intake, and increases in liver and spleen weight. However, it was not affected by dietary krill meal levels. 2. The krill meal diets reduced TNF-α activity as compared to the basal diet after 24 hours (acute phase response) and 1 week(recovery from the acute phase response) following LPS injection (p<0.05). The acute phase response induced a significant increase(p<0.05) in TNF-α activity relative to the control in chicks fed on a basal diet, but this was also unaffected by dietary krill meal levels. 3. Acute phase response-mediated ovotransferrin levels(relative to what was measured in the control bird) were increased in birds fed on the basal, 1.0% and 2.0% krill diets, and were reduced in birds fed on the 0.5 % krill diet. 4. In LPS-injected chicks, 1.0% and 2.0% krill meal diets induced a significant reduction in(p<0.05) the Con A-induced proliferation of PBMC and splenocytes relative to what was observed in the chicks fed on a 0.5% krill diet, whereas the splenocytes proliferated in a linear fashion with the krill levels in the diets of the control birds. The results showed that the dietary levels of krill meal reduced TNF-α activity in the blood and also influenced blood ovotransferrin levels and the proliferation of PBMC and splenocytes, and krill meal is considered to be associated with both innate and cellular immunity in broiler chicks.

콩 추출물 함유 크릴밀 사료가 육계 병아리의 생산성과 TNF-$\alpha$ 및 Ovotransferrin 생합성에 미치는 영향

  • 임진택;박인경;최준영;최도열;이혜정;고태승
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2003.11a
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    • pp.82-83
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    • 2003
  • To study effect of bean extracts to lessen the growth-suppressing-effect of krill meal diet, dietary krill meal with bean extracts on the performance of broiler chicks and proliferation of splenocytes and peripheral blood mononuclear cells(PBMC) and levels of circulating TNF-$\alpha$ and ovotransferrin in plasma was assayed. The krill meal with bean extracts diet lessened the growth-suppressing effect of the krill meal diet. During acute phase responce, the krill meal with bean extracts diet decreased the proliferation of splenocytes and increased the proliferation of the PBMC and reduced the circulating levels of TNF-$\alpha$ and ovotransferrin in plasma. The results Indicated that the krill meal with bean extracts diet related with the acute phase response in broiler chicks.

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