• Title/Summary/Keyword: PAS-4

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Morphology and Histochemistry of the Skin of the Mud Loach, Misgurnus mizolepis, in Relation to Cutaeneous Respiration

  • Park, Jong-Young;Kim, Ik-Soo;Kim, So-Young
    • Animal cells and systems
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    • v.5 no.4
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    • pp.303-308
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    • 2001
  • The skin structure of Misgurnus mizolepis was studied based on the microanatomical investigation of skin fragments taken from four regions. The epidermis was distinguished by two types of skin glands, a small mucous cell and a large club cell. The mucous cell was acid sulfomucins (some sialomucins) but the club cell did not give any histochemical tests for mucosubstances. The presence of a well defined lymphatic system with small lymphocytes was established in the stratum germinativum layer of the epidermis. A large number of blood capillaries run very close to each other just below the basement membrane, and a definite area giving AB and PAS positive was present between the basement membrane and scale. These structural features of skin in M. mizolepis seem to be closely related with cutaneous respiration.

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GENETICAL STUDIES ON NATIVE CHICKENS IN INDONESIA

  • Yamamoto, Y.;Namikawa, T.;Okada, I.;Nishibori, M.;Mansjoer, S.S.;Martojo, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.4
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    • pp.405-410
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    • 1996
  • Phylogenetic analyses were carried out using four Indonesian native chicken breeds; Kampung, Bangkok, Pelung and Kedu. Gene frequencies of four blood group (A, B, D and E) and eight electrophoretic loci (akp, Akp-2, Es-1, Amy-1, Alb, Tf, Pas and Pa-1) were examined. Geographical and breed specific trends in the gene frequencies were not found in the local population of Kampung breed or in four native breeds. The values of average heterozygosity were estimated as 0.35-0.45. Genetic distances among the local populations of Kampung breed and other native breeds were comparatively small. In a cluster analysis, the Bangkok breed and Kampung E population showed distance from another cluster. The coefficient of gene differentiation for local populations of Kampung breed was estimated as 0.099.

Ultrastructure of the Integumentary System of the Amberjack, Seriola dumerili (Teleostei: Carangidae), from Korea (잿방어, Seriola dumerili 피부계의 전자현미경적 구조)

  • Jin, Young Guk;Lee, Jae Woo;Lee, Jung Sick
    • Korean Journal of Ichthyology
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    • v.17 no.4
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    • pp.248-254
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    • 2005
  • Integumentary structure of the amberjack, Seriola dumerili, was examined by light and transmission electron microscopy. Integument of the amberjack consists of epidermal and dermal layers. The epidermal thickness is about $28.32{\mu}m$. The epidermal layer could be classified into superficial, intermediate, and basal layers by morphology and position of the supporting cells. Secretory cells of the epidermal layer were classified into mucous cells and club cells in the amberjack. Mucous cells contained acidic mucous and were alcian-blue positive with AB-PAS (pH 2.5) reaction. The club cells contained well-developed rough endoplasmic reticula and large central vacuole. Also, filament-rich cells were identified in the epidermal layer. The dermal layer consists mainly of collagenous fiber, and it contains fibrocytes and pigment cells. The cytoplasm of fibrocyte had a well-developed rough endoplasmic reticulum. The pigment cells contained electron-dense melanin granules.

A Study on Ballet Costume II -Focusing on Romantic Ballet- (발레의상에 대한 연구 II -로맨틱발레를 중심으로-)

  • 이영숙
    • The Research Journal of the Costume Culture
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    • v.4 no.3
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    • pp.447-464
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    • 1996
  • In this study, observations were made on costumes of romantic ballet. Twelve ballets of romantic period were selected for this study; La Sylphide(1832), La Filledu Danube (1836), Le Diable Boiteux(1836), La Gitana(1838), La Gipsy(1839), La Tarentule(1839), L'Ombre(1839), Giselle(1841), La Peri(1843), La Esmeralda(1844), Pas de Quatre(1845), la Fille de Marbe(1847). Romantic age was acceptance of the ballerinas as the central figure on the stage. Famous ballerinas in romantic age were Marie Taglioni, Fanny Elβler, Carlota Grisi, Fanny Cerrito, Lucile Grahn. Romantic style of dancing sprung onto the stage of the Paris Oepra with M. Taglione's first performance of La Sylphide in 1832. Ballet costumes n romantic period except La Gipsy were with low decolletage, very short transparent sleeves that decorated with shining band or small cuffs, and the waist of the fitted bodice dipped to point in front decorated with sash or jewel. The form of skirts was bell-shaped and reached middle calf. That style became stereotype for ballet costume and called ballet blanc.

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오염지하수정화를 위한 반응벽체의 설계 및 시공

  • 이동호;지원현;이재원;박준범
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.434-437
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    • 2004
  • 본 반층벽체 적용지역의 경우, 침출수의 영향으로 암모니아성 질소의 농도가 최대 29.12mg/L까지 검출되고 있으며 반응물질은 암모니아성 질소제거에 적합한 제올라이트를 선정하였다. 투수성 및 다짐강도를 고려하여 왕사와 50:50의 비율로 혼합, 적용하였으며 지하수 평균유속은 0.0864m/d이고 평균지하수위는 원지반 기준 - 4.0m의 분포를 나타내었다. 지질조사 결과, 적용구간의 지질구조는 매립층, 풍화토, 풍차암, 연암의 순서로 분포하고 있으며, 반응벽체의 설계깊이는 현장 시추조사 결과를 바탕으로 지하수의 Under-pass를 방지하기 위하여 연암층 50cm까지 관입시키도록 설계하였다. 최종적으로 선정된 반응벽체의 규모는 35m(길이) $\times$ 1.2m(두께) $\times$ 8.5 ~ 9.42m(구간별 깊이)로 설계에 반영되었다. 현재, 지하수감시정을 이용한 모니터링 결과, 상부지하수 감시정에서의 오염원은 우기의 영향으로 간헐적으로 발생하고 있으나 하부 지하수 감시정에서의 암모니아성 질소농도는 불검출 되거나 0.5 mg/L 이하로 유지되고 있다.

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Studies on the Population of Toxigenic Fungi in Foodstuffs (III) (각종 실품중의 유독성 진균에 관한 연구 3)

  • Koh, Choon-Myung;Choi, Tae-Joo;Lew, Joon
    • Korean Journal of Microbiology
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    • v.10 no.4
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    • pp.191-194
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    • 1972
  • There were observed the host-parasite relationship between rice grains and contaminating fungi indicated by the fungal penetration degree in the tested rices. The results were as follows ; 1. The fungal penetration could be observed in the outer layer of the rices but couldn't be seen in the starch portin of the tested rices. 2. The Gram staining method was better than the PAS staining method for the observation of fungi penetration into the rices.

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HISTOCHEMICAL STUDY ON THE ORAL MUCOSA OF METHIONINE ADMINISTERED RATS (Methionine투여의 백서구강점막에 관한 조직학적연구)

  • Lee, Chong-Hong
    • The Journal of the Korean dental association
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    • v.9 no.11
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    • pp.673-677
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    • 1971
  • The buccal mucosa was observed histochemically in male rats which were injected with methionine. The staining methods were proceed by means of periodicacid-Schiff reaction, toluidine blue stain, alloxan-Schiff reaction, Mallory's aniline blue stain, Lillie's modification of Bielschowsky method and hematoxylin-eosin stain. The results of the experiment were summarized as follows: 1) PAS reactions of basement membrane and lamina propria increased after 3 and 5 days of methionine administration. 2) Metachromasia of stratum spinosum increased after 3 days of methionine administration. 3) Alloxan-Schiff reactions of stratum granulosum and stratum spinosum increased after 7 days of methionine administration. 4) In the lamina propria, aniline blue staining of collagenous fibers increased after 7, 10 and 14 days of methionine administration.

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Studies on the Effects of Dibutyryl Cyclic AMP and Theophylline on Intracellular Contents of Glycogen of Mouse Follicular Oocytes in Vitro (Dibutyryl Cyclic AMP와 Theophylline이 培養중인 생쥐 濾胞卵子의 Glycogen 함량에 미치는 영향에 관한 연구)

  • Cho, Wan-Kyoo;Yoon, Yong-Dal
    • The Korean Journal of Zoology
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    • v.18 no.1
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    • pp.27-40
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    • 1975
  • dbcAMP와 theophylline이 난자의 성숙을 억제하는 기작과 난자내 glycogen 함량과의 관계를 밝혀보기 위하여 실험한 결과는 다음과 같다. 1. 생쥐 여포난자의 PAS 양성물빌은 glycogen이며 핵의 성숙분열이 진행됨에 따라 glycogen의 함량은 감소한다. 2. dbcAMP나 theophylline에 의해 핵의 성숙이 억제된다 하더라도 난자내의 glycogenolysiss는 촉진된다. 이에 반해 핵분열이 일어나지 않은 난자는 배양이 진행되더라도 glycogen을 그대로 유지하고 있다. 일단 dbcAMP나 theophylline에 의해 성숙이 억제되었던 난자가 성숙과정에 들어가려면 다시 glyconeogenesis가 일어나 세포질내의 glycogen의 양이 회복하며 이때의 glycogen은 핵성숙과 더불어 소모된다. 3. Glycogen의 회복은 배양액내의 glucose 유무에 관계없이 이루어지며 따라서 이는 난자내 glucose 혹은 다른 전구물질에 의해 이루어지리라고 추측된다. 4. 결국 dbcAMP나 theophylline은 난자내 cAMP의 양을 증가시켜 glycogen의 소모를 일으키는 것으로 생각되며, glycogenolysis와 난자의 핵 성숙과정이 별개로 진행되기는 하지만 성숙의 요건은 일정량 이상의 glycogen이 함유되어 있어야 한다는 것을 알게되었다.

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The development of membranes for high temperature PEMFC

  • Lee, Doo-Yeon;Sun, Hee-Young;Cho, Chung-Kun;Lee, Myung-Jin;Seung, Do-Young
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.184-184
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    • 2006
  • We have succeeded in the preparation of high molecular weight polybenzimidazoles by solution polycondensation of 3,3'-diaminobenzidine tetrahydrochloride with isophthalic acid, terephthalic acid, or with their derivatives using polyphosphoric acid both as solvent and as condensing agent. Also, we modified phosphoric acid into fluoroalkyl-phosphonic acids[F-PA]. The main reasons are as follows, first of all F-PAs are stronger acids than PA and alkylphosphonic acids which should promote proton hopping and transport. In addition, F-PA has weaker adsorption onto Pt which help to prevent electrocatalyst poisoning and promote higher oxygen reduction activity. The ionic conductivity of 85%-H3PO4 doped membranes show $10^{-2}\;Scm^{-1}\;to\;3{\times}10^{-2}\;Scm^{-1}\;at\;150^{\circ}C$ MEA with 2 %-added electrolyte shows slightly higher cell voltage than the others.

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Morphological studies on the uterine development of fetuses and neonates in Korean native goats (한국재래산양의 태아 및 신생아의 자궁의 발달에 관한 형태학적 연구)

  • Jung, Soon-hee;Kim, Chong-sup;Kwak, Soo-dong
    • Korean Journal of Veterinary Research
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    • v.38 no.2
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    • pp.258-264
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    • 1998
  • The morphological development of uterus in 60-, 90-, 120-day-old fetuses at gestation and neonates of Korean native goats were investigated by light microscopy. 1. In the 60-day-old fetuses, uterine walls differentiated and divided into the endometrium, myometrium and perimetrium. 2. In the 90-day-old fetuses, the blood vessels appeared in the uterine walls, in the 120-day-old fetuses the nodules (primordial caruncles) appeared first in the endometrium. 3. In the 120-day-old fetuses, the gland primordia observed on the uterine epithelium. In the neonates, the developing uterine glands were more number and deeper than those of 120-old fetuses. 4. PAS reaction of the mucosa cells in the endometrium was reacted weak, moderate and strong at 90-, 120-day-old fetuses and neonates, respectively.

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