• Title/Summary/Keyword: PAMP

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NPR1 is Instrumental in Priming for the Enhanced flg22-induced MPK3 and MPK6 Activation

  • Yi, So Young;Min, Sung Ran;Kwon, Suk-Yoon
    • The Plant Pathology Journal
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    • v.31 no.2
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    • pp.192-194
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    • 2015
  • Pathogen-associated molecular patterns (PAMPs) activate mitogen-activated protein kinases (MAPKs), essential components of plant defense signaling. Salicylic acid (SA) is also central to plant resistance responses, but its specific role in regulation of MAPK activation is not completely defined. We have investigated the role of SA in PAMP-triggered MAPKs pathways in Arabidopsis SA-related mutants, specifically in the flg22-triggered activation of MPK3 and MPK6. cim6, sid2, and npr1 mutants exhibited wild-type-like flg22-triggered MAPKs activation, suggesting that impairment of SA signaling has no effect on the flg22-triggered MAPKs activation. Pretreatment with low concentrations of SA enhanced flg22-induced MPK3 and MPK6 activation in all seedlings except npr1, indicating that NPR1 is involved in SA-mediated priming that enhanced flg22-induced MAPKs activation.

Reserch and development of metal halide lamp for LCD projector back light (LCD PROJECTOR BACK LIGHT용 METAL HALIDE LAMP의 연구 및 개발)

  • 박창식;정의선;이승수
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 1995.10a
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    • pp.39-46
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    • 1995
  • Metal halide pamp for LCD projector back light is high technologlcal product with its consumption increasing by 30% a year according to LCD merchandise and we are whollydepending on imprtation. At this time exper iment. we achleved those results as below. 1. Reallzation of domestic production of 150W metal halide lamp for LCD progector back light and electrical ballatst. 2. Possession of self-designing and manufacturing technology of submlnlature metal halide lamp. 3. Possession of deslgning technology of electrlcal ballast for metal hallde lamp.

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Study on the Manufacturing Process of Artemisia asiatica Carbonized Organic Solution(Wormwood Acetic Acid) by Development of Carbonize Equipment (탄화장치 개발에 의한 쑥(Artemisia asiatica) 탄화 유기용액(쑥초액) 제조에 관한 연구)

  • Ryu, Soung-Ryual
    • Journal of Integrative Natural Science
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    • v.4 no.1
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    • pp.15-22
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    • 2011
  • In this study, liquid with carbonized artemisia was developed for the first time that can be used as food additives and outside medicinal agents by burning artemisia species such as Artemisia dubia wall, Artemisia capillaris Thunb, Artemisia montana (Nakai) Pamp), Artemisia keiskeana Miq, Leonurus Japonicus Houtt. etc. In addition, edibility of recovered liquid with carbonized artemisia was verified by comparing the contents according to each ingredient with common refined pyroligneous liquid through the analysis of 9 kinds of organic acid by the species of artemisia, methanol and ethanol. Besides, the result of antimicrobial activities on six kinds of food spoilage and food poisoning bacteria observed by paper disc method for liquid with carbonized artemisia indicated that those two liquids showed strong antimicrobial. At the concentration of 1.5 mg/disc, clear zone for S. sonnei and S. aureus, L. monocytogenes was 13~16 mm, and they showed stronger antimicrobial activities than other strain.

NLRP3 Inflammasome as Therapeutic Targets in Inflammatory Diseases

  • Annamneedi Venkata Prakash;Il-Ho Park;Jun Woo Park;Jae Pil Bae;Geum Seon Lee;Tae Jin Kang
    • Biomolecules & Therapeutics
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    • v.31 no.4
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    • pp.395-401
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    • 2023
  • Innate immunity is a first line defence system in the body which is for sensing signals of danger such as pathogenic microbes or host-derived signals of cellular stress. Pattern recognition receptors (PRR's), which present in the cell memebrane, are suspect the infection through pathogen-associated molecular patterns (PAMP), and activate innate immunity with response to promote inflammation via inflammatory cells such as macrophages and neutrophils, and cytokines. Inflammasome are protein complexes which are part of innate immunity in inflammation to remove pathogens and repair damaged tissues. What is the important role of inflammation in disease? In this review, we are focused on the action mechanism of NLRP3 inflammasome in inflammatory diseases such as asthma, atopic dermatitis, and sepsis.

The New Record of Pleotrichophorus pseudoglandulosus (Palmer, 1952) (Hemiptera: Aphididae) in South Korea (한국의 미기록종 Pleotrichophorus pseudoglandulosus (Palmer, 1952)(노린재목: 진딧물과)에 대한 보고)

  • Hyobin Lee;Deog-Kee Park;Ki-Jeong Hong;Wonhoon Lee
    • Korean journal of applied entomology
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    • v.62 no.4
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    • pp.295-298
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    • 2023
  • Pleotrichophorus pseudoglandulosus (Palmer, 1952) collected on Artemisia princeps Pamp. is reported for the first time in South Korea. Species description, measurement, diagnosis, distributions, host plants, illustrations, and identification key of apterous viviparous females in the genus Pleotrichophorus from South Korea are provided.

Comparison of Agronomic Characteristics, Forage Quality and Productivity in Local Collections of Mugwort (Artemisia princeps. Pamp.) (수집종 쑥(Artemisia princeps. Pamp.)의 생육특성, 사료가치 및 수량성 비교)

  • Shin, Chung Nam;Ko, Ki Hwan;Kim, Jong Duk;Lee, Jeong Hoon;Kim, Kwang Sik
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.4
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    • pp.277-282
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    • 2017
  • This study was conducted to evaluate on agronomic characteristics, forage quality and dry matter (DM) yields of mugwort (Artemisia princeps Pamp.) collected different region at Seongju in Kyeongbuk from 2014 to 2015. The experiment was arranged in randomized complete block design with three replications. Mugwort was planted on $17^{th}$ March, 2014 and harvested on $13^{th}$ September, 2014 and on $25^{th}$ May and $9^{th}$ September, 2015. DM yields of Dalsung(1), Seongju B.(1), Dalsung(2) and Sajuarissuk were 17.3, 16.8, 16.7 and $16.7(MT\;ha^{-1})$, respectively and the DM yields of those were higher (p<0.05) than the other mugworts in 2014. In 2015, DM yields of Dalsung(1), Dalsung(2), Seongju B.(1), and Sajuarissuk were 19.5, 19.3, 18.4 and $18.4(MT\;ha^{-1})$, respectively and DM yields of those were higher (p<0.05) than the others. DDM(digestable dry matter) content of mugworts was a range of 56.4 to 60.8% and that of Sajabalssuk, 60.8% was the highest. Seongju B.(1) 58.8% was higher than Seongju B.(2) 56.4% but there were no difference among the Dalsung(1) 58.4%, Dalsung(2) 58.1%, Sajuarissuk 57.7% and Yeongcheon. The result of this study indicated that Dalsung(1), Dalsung(2), Sajuarissuk and Seongju B.(1) would be recommendable for forage production.

Magnaporthe oryzae Effector AVR-Pii Helps to Establish Compatibility by Inhibition of the Rice NADP-Malic Enzyme Resulting in Disruption of Oxidative Burst and Host Innate Immunity

  • Singh, Raksha;Dangol, Sarmina;Chen, Yafei;Choi, Jihyun;Cho, Yoon-Seong;Lee, Jea-Eun;Choi, Mi-Ok;Jwa, Nam-Soo
    • Molecules and Cells
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    • v.39 no.5
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    • pp.426-438
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    • 2016
  • Plant disease resistance occurs as a hypersensitive response (HR) at the site of attempted pathogen invasion. This specific event is initiated in response to recognition of pathogen-associated molecular pattern (PAMP) and subsequent PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI). Both PTI and ETI mechanisms are tightly connected with reactive oxygen species (ROS) production and disease resistance that involves distinct biphasic ROS production as one of its pivotal plant immune responses. This unique oxidative burst is strongly dependent on the resistant cultivars because a monophasic ROS burst is a hallmark of the susceptible cultivars. However, the cause of the differential ROS burst remains unknown. In the study here, we revealed the plausible underlying mechanism of the differential ROS burst through functional understanding of the Magnaporthe oryzae (M. oryzae) AVR effector, AVR-Pii. We performed yeast two-hybrid (Y2H) screening using AVR-Pii as bait and isolated rice NADP-malic enzyme2 (Os-NADP-ME2) as the rice target protein. To our surprise, deletion of the rice Os-NADP-ME2 gene in a resistant rice cultivar disrupted innate immunity against the rice blast fungus. Malic enzyme activity and inhibition studies demonstrated that AVR-Pii proteins specifically inhibit in vitro NADP-ME activity. Overall, we demonstrate that rice blast fungus, M. oryzae attenuates the host ROS burst via AVR-Pii-mediated inhibition of Os-NADP-ME2, which is indispensable in ROS metabolism for the innate immunity of rice. This characterization of the regulation of the host oxidative burst will help to elucidate how the products of AVR genes function associated with virulence of the pathogen.

Effect of Mixed Planting Ratios of Pteris multifida Poir. and Artemisia princeps Pamp. on Phytoremediation of Heavy Metals Contaminated Soil (중금속 오염토양 정화에 영향을 미치는 봉의꼬리(Pteris multifida Poir.)와 쑥(Artemisia princeps Pamp.)의 혼합식재 비율)

  • Kwon, Hyuk Joon;Jeong, Seon A;Shin, So Lim;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.30 no.2
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    • pp.160-166
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    • 2017
  • This study was performed to develop the efficient phytoremediation model in the paddy soil contaminated with heavy metals by cultivating Pteris multifida and Artemisia princeps with different mixing ratios (1:0, 8:1, 6:1, 4:1). As a result of investigating the heavy metal accumulation of each plant per dried material (1 kg), content of arsenic and cadmium was the highest in aerial part of P. multifida (169.82, $1.70mg{\cdot}kg^{-1}DW$, each) among the treated group. Lead content was the highest ($12.58mg{\cdot}kg^{-1}DW$) in the aerial part of P. multifida cultivated with 8:1 mixed planting. But the content of copper and zinc was the highest (33.94, $61.78mg{\cdot}kg^{-1}DW$, each) in the aerial part of A. princeps with 8:1 treatment. Regardless of heavy metals, plant uptake from the $1m^2$ soil was the highest in 4:1 mixed planting group, which showed the best yield of A. princeps.

Immunomodulating Activity of Fungal $\beta$-Glucan through Dectin-1 and Toll-like Receptor on Murine Macrophage

  • Kim, Ha-Won
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2006.11a
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    • pp.103-115
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    • 2006
  • $\beta$-Glucan is a glucose polymer that has linkage of $\beta$-(1,3), -(1,4) and -(1,6). As exclusively found in fungal and bacterial cell wall, not in animal, $\beta$-glucans are recognized by innate immune system. Dendritic cells (DC) or macrophages possesses pattern recognition molecule (PRM) for binding $\beta$-glucan as pathogen-associated molecular pattern (PAMP). Recently $\beta$-glucan receptor was cloned from DC and named as dectin-l which belongs to type II C-type lectin family. Human dectin-1 is consisted of 7 exons and 6 introns. The polypeptide of dectin-1 has 247 amino acids and has cytoplasmic, transmembrane, stalk and carbohydrate recognition domains. Dectin-1 could recognize variety of beta-1,3 and/or beta-1,6 glucan linkages, but not alpha-glucans. In our macrophage cell line culture system, dectin-1 mRNA was detected in RA W264.7 cells by reverse transcription-polymerase chain reaction (RT-PCR). Dectin-1 was also detected in the murine organs of spleen, thymus, lung and intestines. Treatment of RA W264.7 cells with $\beta$-glucans of Ganoderma lucidum (GLG) resulted in increased expression of IL-6 and TNF-$\alpha$ in the presence of LPS. However, GLG alone did not increase IL-6 nor TNF-$\alpha$. These results suggest that receptor dectin-1 cooperate with CD14 to activate signal transduction that is very critical in immunoresponse.

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Gene Expression and Regulation of Wax Moth Transferrin by PAMPs and Heavy Metals

  • Han, Jik-Hyon;Lee, Ji-Sook;Lee, Chang-Seok;Koh, Sang-Kyun;Seo, Sook-Jae;Yun, Chi-Young
    • Animal cells and systems
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    • v.13 no.3
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    • pp.297-304
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    • 2009
  • A complete mRNA sequence of transferrin from the wax moth, Galleria mellonella, was obtained, and compared with those of other species. We previously reported that the sequence was most similar to those of Manduca sexta and Bombyx mori. As in other moths, G. mellonella transferrin had only one iron-binding site at its N-terminal region. Semi-qRT PCR was conducted to investigate tissue-specific distribution and transcriptional regulation of the wax moth transferrin mRNA. Larval muscle and fat body contained larger quantity of mRNA than other tested tissues. In this study, it was observed that iron and cadmium regulated transferrin transcription, and this regulation pattern was tissue specific. Iron up-regulated transferrin mRNA level in fat body, while suppressed it in the Malpighian tubules and silk glands. Cadmium decreased the mRNA level in fat body, muscle, and Malpighian tubules, but significantly increased the mRNA level in silk glands. In addition, the mRNA expression was induced by all tested pathogen-associated molecular patterns (PAMPs) including LPS, lipoteichoic acid (LTA), glucan, and even chitin.