• Journal of Nutrition and Health
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• v.49 no.4
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• pp.241-246
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• 2016

Purpose: Aloe-emodin (AE), an ingredient of aloe, is known to exhibit anti-inflammatory activities. However, little is known about the underlying molecular mechanisms of its inflammatory modulatory activity in vitro. In the present study, we investigated the anti-inflammatory potential of AE using $Pam_3CSK_4$-stimulated macrophages. Methods: RAW 264.7 macrophages were treated with AE (0~20 mM) for 1 h, followed by treatment with $Pam_3CSK_4$ for 1 h. After incubation, mRNA expression levels of cytokines were measured. The effect of AE on TLR2-related molecules was also investigated in $Pam_3CSK_4$-stimulated RAW 264.7 macrophages. Results: AE attenuated $Pam_3CSK_4$-stimulated expression of proinflammatory cytokines, including tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-6 (IL-6), and interleukin-$1{\beta}$ ($IL-1{\beta}$) in RAW 264.7 macrophages. Two concentrations of AE ($10{\mu}M$ and $20{\mu}M$) effectively reduced mRNA expression of TLR2 by 41.18% and 54.43%, respectively, compared to that in control cells (p < 0.05). AE also decreased nuclear factor-kappa B ($NF-{\kappa}B$) activation and mitogen-activated protein kinase (MAPK) phosphorylation. Phosphorylation levels of ERK1/2, p38, and JNK were markedly reduced by $20{\mu}M$ AE. In particular, AE decreased phosphorylation of ERK in a dose-dependent manner in $Pam_3CSK_4$-stimulated RAW 264.7 macrophages. Conclusion: Our data indicate that AE exerts its anti-inflammatory effect by suppressing TLR2-mediated activation of $NF-{\kappa}B$ and MAPK signaling pathways in macrophages.

• Journal of Korean Society for Atmospheric Environment
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• v.27 no.5
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• pp.534-544
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• 2011

The secondary aerosol forming potential of ambient air was first measured with the Potential Aerosol Mass(PAM) chamber at Gosan supersite on Jeju island from October 22 to November 5, 2010. PAM chamber is a small flowthrough photo-oxidation chamber with extremely high OH and $O_3$ levels. The OH exposure in the PAM chamber was $(2{\pm}0.4){\times}10^{11}{\sim}(6{\pm}1.2){\times}10^{11}$ molecules $cm^{-3}$ s and was similar to 2 to 5 days of aging in the atmosphere. By periodically turning on and off UV lamps in the PAM chamber, ambient aerosol and newly formed aerosol (e.g. called as PAM aerosol) was alternately measured. Aerosol number and mass concentration in the range of 10~487 nm in diameter was measured by SMPS 3034. With UV lamps on, the nucleation mode particles smaller than 50 nm in diameters were formed. Their number concentration was greater than 105 $cm^{-3}$, leading to increase in aerosol mass by 0~8 ${\mu}gm^{-3}$. The variations of PAM and ambient aerosols were greatly dependent on characteristics of air masses such as precursor concentrations and degree of aging. This preliminary results suggests that PAM chamber is useful to assess the aerosol formation potential of air mass and its impact on the air quality. The further analysis of data with gaseous and particulate measurements will be done.

• The Journal of the Korea Contents Association
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• v.7 no.1
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• pp.116-123
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• 2007

This paper proposed a new amplitude width modulation for OLED data driver IC. The data driver controls brightness of OLED by adjusting amplitude and width of the data drive current pulse. There were two conventional methods; pulse amplitude modulation(PAM) and pulse width modulation(PWM). The PWM method suffered from lower light emitting time efficiency at low luminance signal. The PAM method suffered from large chip area using DACs for each column. The proposed method was aiming at accurately controlling of the current level by MSB data and light emitting efficiency by LSB data to improve the inefficiencies of the PAM and a PWM. The proposed AWM driver circuit implemented using $0.35-{\mu}m$ 3-poly 4-metal CMOS high voltage process. The simulation result shows the improvement in the accuracy of the gray level control even though the driver circuit is smaller than the PAM.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.38 no.4 s.117
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• pp.1-9
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• 2006

Increasing the filler content of sheet provides an opportunity for saving production cost through fiber replacement with relatively low-priced filler. But increasing the filler content tends to decrease the strength of paper and filler retention. To overcome these problems, preflocculation technology of fillers has been suggested. To evaluate the effect cationic polymers on the size and size distribution of preflocculated GCC and their shear stability, cationic PAM and cationic starch were used. Results showed that cationic PAM formed large prefloc at low dosage. It was required to add 15 times as high as cationic starch to cationic PAM to obtain the same size prefloc. But preflocs formed with cationic starch was superior in shear stability to those formed with cationic PAM. With the increase of shear rate the size of preflocs decreased. Greater amount of small preflocs or un-flocculated fillers was observed when the dosage of polymers was low and this ended up low ash retention in handsheets.

• Polymer(Korea)
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• v.27 no.4
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• pp.358-363
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• 2003

Dispersion polymerization of acrylamide was carried out in the media of ethyl alcohol/water mixtures using hydroxypropyl cellulose and ammonium persulfate as steric stabilizer and initiator, respectively. The effects of concentrations of initiator and steric stabilizer, amount of monomer, polymerization temperature, ethyl alcohol/water ratio, and purification of monomer and nitrogen purge on the particle size of the latices and molecular weight of the polymers were investigated. The average particle diameter increased with increasing concentration of initiator, water content in ethyl alcohol/water media, and polymerization temperature, but decreased with monomer and stabilizer concentrations. The viscosity average molecular weight increased with increasing concentrations of monomer, steric stabilizer, and water content in dispersion media, but decreased with initiator concentration and polymerization temperature. The PAM polymers prepared with the purified monomer and the nitrogen purging before the reaction showed the highest molecular weight. In this study, PAM latices of 0.5∼2.4 $\mu\textrm{m}$ with 20000∼335000 in M$\_$v/ were prepared and the resulting PAM latices were all dissolved in water instantly.

• International Journal of Oral Biology
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• v.41 no.1
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• pp.17-23
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• 2016

Chronic/cyclic neutropenia, leukocyte adhesion deficiency syndrome, Papillon-$Lef{\grave{e}}vre$ syndrome, and $Ch{\grave{e}}diak$-Higashi syndrome are associated with severe periodontitis, suggesting the importance of neutrophils in the maintenance of periodontal health. Various Toll-like receptor (TLR) ligands are known to stimulate neutrophil function, including FcR-mediated phagocytosis. In the present study, the effect of TLR2 activation on the non-opsonic phagocytosis of oral bacteria and concomitant production of reactive oxygen species (ROS) by human neutrophils was evaluated. Neutrophils isolated from peripheral blood were incubated with Streptococcus sanguinis or Porphyromonas gingivalis in the presence of various concentrations of $Pam_3CSK_4$, a synthetic TLR2 ligand, and analyzed for phagocytosis and ROS production by flow cytometry and chemiluminescence, respectively. $Pam_3CSK_4$ significantly increased the phagocytosis of both bacterial species in a dose-dependent manner. However, the enhancing effect was greater for S. sanguinis than for P. gingivalis. $Pam_3CSK_4$ alone induced ROS production in neutrophils and also increased concomitant ROS production induced by bacteria. Interestingly, incubation with P. gingivalis and $Pam_3CSK_4$ decreased the amounts of ROS, as compared to $Pam_3CSK_4$ alone, indicating the possibility that P. gingivalis survives within neutrophils. However, neutrophils efficiently killed phagocytosed bacteria of both species despite the absence of $Pam_3CSK_4$. Although P. gingivalis is poorly phagocytosed even by the TLR2-activated neutrophils, TLR2 activation of neutrophils may help to reduce the colonization of P. gingivalis by efficiently eliminating S. sanguinis, an early colonizer, in subgingival biofilm.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.4
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• pp.244-251
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• 2001

Over half of all biologically active peptide and peptide hormones are $\alpha$-amidated at their C-terminus, which is essential for their full biological activities. Amidation is accomplished through the sequential reaction of the two enzymes encoded by the single bifunctional, peptidyl-glycine $\alpha$-amidating monooxygenase (PAM or an $\alpha$-amidating enzyme). PAM catalyze the forma - tion of a peptide amide from peptide precursors that include a C-terminal glycine, and requires copper molecular oxygen and ascorbate. PAM is the only enzyme that produces peptide amides in vivo. However various strategies utilizing PAM, carboxypeptidase-Y enzymes, and chemical syn-thesis have been developed for producing peptide amides in vitro. The growing need and impor-tance of peptide amide drugs has highlighted the necessity for a efficient in vitro amidating sys-tem for industrial application for the production of peptide hormones, like calcitonin and oxytocin. This review presents the current situation regarding amidation with a special emphasis on the in-dustrial production or peptide hormones.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.892-893
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• 2008

본 논문에서는, 기존의 PAM 인버터와는 달리 저 단가 구현 및 높은 성능을 낼 수 PAM 인버터 구조에 대해 고찰한다. 배터리를 전원으로 사용하는 일반적인 인버터의 경우 초기기동 및 저속운전 영역에서는 배터리 전압으로 인해 전류 및 토크 리플이 크며, 배터리 SOC의 최소치 보다 낮은 역기전력을 갖는 전동기만을 사용할 수 있다. 이를 해결하기 위해 2-스위치 Buck-Boost 컨버터와 4-스위치 인버터로 구성된 새로운 PAM 인버터 시스템을 제안한다. 제안된 시스템은 DC 링크 가변을 통해 역기전력이 낮은 저속운전영역에서는 감압하고 반대로 역기전력이 높은 고속운전영역에서는 승압시킬 수 있어 전류 및 토크 리플을 줄일 수 있다. 마지막으로 시뮬레이션을 통하여 제안된 시스템의 적용 가능성을 검증한다.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.3
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• pp.249-255
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• 1995

This study was conducted to investigate the effects of some soil conditioners, such as polyacrylamide(PAM), polyvinylalcohol(PVA) and Bitumen emulsion, on aggregate formation and stability, wetting angle, sorptivity and penetrability of the soil with different textures : sand, sandy loam, loam and clay loam. A pot experiment was carried out to find out the effect of treatment on the germination and growth of lettuce with three textures : sand, sandy loam and silt loam. Soil aggregates larger than 2mm in untreated soils at dry condition were naught in sand, 45% in sandy loam, 80% in loam and 90% in clay loam. Treatments of soil conditioners tended to increase the occurrence of soil aggregate larger than 2mm, which were 20~25% in sand, 55~75% sandy loam, but not affected greatly aggragate occurence in loam and clay loam. The aggregate instability was decreased by the soil conditioner treatment. The wetting angles of the soils were greatly changed by hydrophobic of Bitumen, but those were changed slightly by PVA and PAM application. The sorptivity and penetrability data indicated that the effects of different materials on these parameters differed depending upon soil texture. Application of PVA and PAM were no effect exceptive in sand. Application of Bitumen revealed that water movement was not showed in all soils. The germination rate, root weight and top plant weight of lettuce were increased in all soils by PAM treatment as compared to untreated soils. Particularly the greater effect was occurred in sand soil than in sandy loam and silt loam. PAM increased greatly the moisture content and air phase of soils.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.336-336
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• 2011

본 연구에서는 강우시 경사지 밭에서 발생하는 토양유실량을 저감하기 위하여 지표피복재의 유무와 강우강도를 달리하여 실외인공강우 실험을 통하여 평가하였다. 인공강우 실험은 경사도 28%와 각 시험포는 $5\times30m$의 크기로 strawmat+PAM+sawdust, control(bare), strawmat+PAM, strawmat+PAM+rice husks를 4개 조성하고, 각 시험포는 하단부에 강우유출수를 채취할 수 있는 설비(flume)를 설치하였다. 대조구 실험을 위하여 각 시험포에서 발생하는 직접유출량과 유사량을 비교 분석하였다. 시험포의 토양은 양질사토로 일반 고랭지 밭의 흙과 동일한 성질을 가지고 있는 토양을 사용하였고, 지표 피복처리는 straw mat, saw dust, rice husk 그리고 PAM 등 4개의 재료를 사용하였다. 인공강우 실험은 1차 10월 18일, 2차 10월 24일, 3차 10월 30일, 4차 11월 7일로 총 21일간 각각 약 1주일의 간격을 두고 실시하였으며 인공강우 시험기의 강우강도는 각 31.1 ~ 44.4 mm/hr로 모의하였다. 연구결과 시험포에서 발생한 유출량은 지표피복을 하지 않은 대조구 시험포보다 지표를 피복한 시험포에서 약 15.7 ~ 19%의 감소한 것으로 나타났다. 유사량은 대조구 시험포보다 약 93.1 ~ 99.3% 감소한 것으로 나타났으며, 유출량과 유사량은 straw mat+PAM+sawdust와 straw mat+PAM+rice husk를 적용한 시험포에서 저감효과가 큰 것으로 나타났다. 이와 같이 지표피복처리는 강우가 발생하였을 때 강우타격력의 감소와 유출수의 유속감소 그리고 침투능을 증가하는 역할을 하여 경사지, 농경지 및 공사현장에서 토양 유실을 저감하며, 하천이나 강으로 유입되는 탁수량의 저감에 큰 효과가 있을 것으로 판단된다.