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Effects of high-pressure processing on taste-related ATP breakdown compounds and aroma volatiles in grass-fed beef during vacuum aging

  • Utama, Dicky Tri;Lee, Seung Gyu;Baek, Ki Ho;Jang, Aera;Pak, Jae In;Lee, Sung Ki
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.8
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    • pp.1336-1344
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    • 2018
  • Objective: This study aimed to observe whether high-pressure processing (HPP) affected aroma development and the degradation rate of umami taste-related ATP breakdown products, specifically inosinic acid in grass-fed beef during vacuum aging. Methods: Strip loin (longissimus lumborum) cuts obtained from six grass-fed Friesian Holstein steers (32 months old) on day 4 post slaughter were vacuum-packed and subjected to pressurization at 300 and 500 MPa for 180 s at $15^{\circ}C{\pm}2^{\circ}C$. The samples were then stored for 4 weeks at $5^{\circ}C{\pm}0.5^{\circ}C$ under vacuum and compared with the control (0.1 MPa). Results: HPP increased the shear force value, promoted moisture loss and lipid oxidation, induced surface paleness, stabilized pH during aging, and reduced bacterial load and growth. The shear force value of 500 MPa-treated samples remained higher than the control after aging, while no significant differences were found between the control and 300 MPa-treated samples. Degradation of inosinic acid and inosine occurred during pressurization, resulting in an increase in hypoxanthine content. However, the degradation rate in HPP-treated samples during aging was slower; therefore, inosinic acid and inosine content remained higher than in control samples. No significant differences were found in hypoxanthine content at the end of aging. HPP intensified the levels of hexanal, octanal, 2-methylbutanal, 3-methylbutanal, benzaldehyde, and 2,5-dimethylpyrazine in cooked-aged beef samples. Conclusion: HPP induced aroma development and delayed the degradation of inosinic acid. However, it also reduced the postmortem tenderization rate.

An Analysis on Global Terminal Operator's Selection of Container Terminal -Focusing on the Chinese Container Ports- (GTO의 신규터미널 후보지 선택에 관한 연구 -중국 컨테이너 항만을 중심으로-)

  • Yeo, Gi-Tae;Jung, Hyun-Jae;Pak, Ji-Young
    • Journal of Korea Port Economic Association
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    • v.28 no.1
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    • pp.159-178
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    • 2012
  • Recently, Global network expansion strategy of GTOs(Global Terminal Operator) coupled with each country's port policy, plays huge role for the evolution of modern container port. The Chinese ports can be regarded as the major markets to the GTOs. However, there are scant of researches for finding the key success factors of GTOs' strategies when they consider to invest in overseas. In this respect, the aims of this study were to draw out the evaluation variables for successful investment strategies of GTOs, and to calculate the selected target ports. The 14 variables are selected including the variable named 'development potentiality of a port' through literature reviews. Using the Factor Analysis (FA) based on selected variables, four principal factors were extracted such as 'ability for port operating and cargo generating', 'the trade route and volume', 'the calling potentiality for large vessels' and 'the possibility of utilization of existing infrastructure'. In addition, the weights of factors and variables are evaluated through Fuzzy AHP method. As a result, 'ability for port operating and cargo generating' is chosen as the most important factor among principal factors as scored 0.343, and 'the development potentiality of a port' (0.107) is represented as the most important variable among 14 detailed variables. In overall, from the Global Terminal Operator's point of view, Shanghai is ranked as most suitable port for operating new terminal among the top 5 Chinese ports.

Anti-inflammatory Effect of Onion (Allium cepa) Peel Hot Water Extract in vitro and in vivo (양파껍질 열수추출물의 in vitro 및 in vivo 항염증 효과)

  • Kang, Bo-Kyeong;Kim, Koth-Bong-Woo-Ri;Ahn, Na-Kyung;Choi, Yeon-Uk;Kim, Min-ji;Bark, Si-Woo;Pak, Won-Min;Kim, Bo-Ram;Park, Ji-Hye;Bae, Nan-Young;Ahn, Dong-Hyun
    • KSBB Journal
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    • v.30 no.4
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    • pp.148-154
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    • 2015
  • Onion (Allium cepa) is one of the flavonoids-rich materials in human diet and onion peel, which is the onion by-products, contains over 20 times more quercetin than the flesh. In this study, to examine the anti-inflammatory effects of onion peel hot water extract (OPHWE), the cell viability, nitric oxide (NO), pro-inflammatory cytokines, such as interluekin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and IL-$1{\beta}$, were measured using the murine macrophage cell line RAW 264.7 cells. The Balb/c mice were used for an in vivo acute toxicity test and ICR mice were used for measurement of inhibition effects of croton oil-induced mouse ear edema. As a result, NO levels decreased in a dose-dependent manner. The production of IL-6, TNF-${\alpha}$, and IL-$1{\beta}$ was suppressed by 38%, 41%, and 34% respectively, compared with that of the LPS only group, without any cytotoxicity. The edema formation in the ICR mouse ear was also reduced compared to that in control. Moreover, there were no mortalities occurred in mice administered 5,000 mg/kg body weight of OPHWE. These results suggest that OPHWE has considerable anti-inflammatory activities and can be regarded as a potent candidate material to treat inflammatory diseases.

Nonlinear Thermo-mechanical Analysis Considering Heat Flow under Fire Conditions (화재 열 유동을 고려한 구조물의 열응력해석)

  • Pak, Hongrak;Kang, Jun Won;Lee, Jinwoo
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.29 no.4
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    • pp.369-376
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    • 2016
  • In this study, a numerical analysis framework for investigating the nonlinear behavior of structures under fire conditions is presented. In particular, analysis procedure combining fire-driven flow simulation and thermo-mechanical analysis is discussed to investigate the mechanical behavior of fire-exposed representative volume structures made of steel and concrete, respectively. First of all, fire-driven flow analysis is conducted using Fire Dynamics Simulator(FDS) in a rectangular parallelepiped domain containing the structure. The FDS simulation yields the time history of temperature on the surface of the structure under fire conditions. Second, mechanical responses of the fire-exposed structure with respect to prescribed uniformly distributed loads are calculated by a coupled thermo-mechanical analysis using the time-varying surface temperature as boundary conditions. Material nonlinearities of steel and concrete have been considered in the thermo-mechanical analysis. A series of numerical results are presented to demonstrate the feasibility of the multiphysics structural fire analysis for investigating the structural behavior under fire conditions.

Modelling the Effects of Temperature and Photoperiod on Phenology and Leaf Appearance in Chrysanthemum (온도와 일장에 따른 국화의 식물계절과 출엽 예측 모델 개발)

  • Seo, Beom-Seok;Pak, Ha-Seung;Lee, Kyu-Jong;Choi, Doug-Hwan;Lee, Byun-Woo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.18 no.4
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    • pp.253-263
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    • 2016
  • Chrysanthemum production would benefit from crop growth simulations, which would support decision-making in crop management. Chrysanthemum is a typical short day plant of which floral initiation and development is sensitive to photoperiod. We developed a model to predict phenological development and leaf appearance of chrysanthemum (cv. Baekseon) using daylength (including civil twilight period), air temperature, and management options like light interruption and ethylene treatment as predictor variables. Chrysanthemum development stage (DVS) was divided into juvenile (DVS=1.0), juvenile to budding (DVS=1.33), and budding to flowering (DVS=2.0) phases for which different strategies and variables were used to predict the development toward the end of each phenophase. The juvenile phase was assumed to be completed at a certain leaf number which was estimated as 15.5 and increased by ethylene application to the mother plant before cutting and the transplanted plant after cutting. After juvenile phase, development rate (DVR) before budding and flowering were calculated from temperature and day length response functions, and budding and flowering were completed when the integrated DVR reached 1.33 and 2.0, respectively. In addition the model assumed that leaf appearance terminates just before budding. This model predicted budding date, flowering date, and leaf appearance with acceptable accuracy and precision not only for the calibration data set but also for the validation data set which are independent of the calibration data set.

Early-type Dwarf Galaxies in the Virgo Cluster: An Ultraviolet Perspective

  • Kim, Suk;Rey, Soo-Chang;Sung, Eon-Chang;Lisker, Thorsten;Jerjen, Helmut;Lee, Youngdae;Chung, Jiwon;Pak, Mina
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.81-81
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    • 2012
  • Since the ultraviolet (UV) flux of an integrated population is a good tracer of recent star formation activities, UV observations provide an important constraint on star formation history (SFH) in galaxies. We present UV color-magnitude relations (CMRs) of early-type dwarf galaxies in the Virgo cluster, based on Galaxy Evolution Explorer (GALEX) UV data and the Extended Virgo Cluster Catalog (EVCC, Kim, S. in prep.). The EVCC covers an area 5.4 times larger (750 deg2) than the footprint of the classical Virgo cluster catalog by Binggeli and collaborators. We secure 1304 galaxies as members of the Virgo cluster and 526 galaxies of them are new objects not contained in the VCC. Morphological classification of galaxies in the EVCC is based on the optical image ("Primary Classification") and spectral feature ("Secondary Classification") of the SDSS data. We find that dwarf lenticular galaxies (dS0s) show a surprisingly distinct and tight locus separated from that of ordinary dwarf elliptical galaxies (dEs), which is not clearly seen in previous CMRs. The dS0s in UV CMRs follow a steeper sequence than dEs and show bluer UV-optical color at a given magnitude. Most early type dwarf galaxies with blue UV colors (FUV-r < 6 and NUV-r < 4) are identified as those showing spectroscopic hints of recent or ongoing star formation activities. We explore the observed CMRs with population models of a luminosity-dependent delayed exponential star formation history. The observed CMR of dS0s is well matched with models with relatively long delayed star formation. Our results suggest that dS0s are most likely transitional objects at the stage of subsequent transformation of late-type progenitors to ordinary red dEs in the cluster environment. In any case, UV photometry provides a powerful tool to disentangle the diverse subpopulations of early-type dwarf galaxies and uncover their evolutionary histories.

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A Study on the Safety Handling Method of KCG's Water Jet Propulsion Ship (해양경찰 Water Jet 추진함정의 안전 조함법 연구)

  • Yun, Chong-Gum;Pak, Chae-Hong;Park, Deuk-Jin;Jung, Cho-Yeong
    • Journal of Navigation and Port Research
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    • v.41 no.6
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    • pp.373-380
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    • 2017
  • Operational errors caused by human factors, which is the major cause of marine accidents, include lack of knowledge, misunderstanding knowledge, and inadequate procedures. Recently, the type of propulsion mounted on KCG cutters has been diversified. In particular, the water jet propulsion unit, which was mainly installed in small boats, have been gradually expanded to medium and large size Coast Guard cutters, reaching 50% of the total. Axes types are divided into 2 to 4, and the bucket types are divided into Double Reverse Bucket(DRB) and Single Reverse Bucket(SRB); in these, the backward and steering control methods are completely different. Diversification of these operating systems can increase factors causing human error by the ships' operators. However, there is a lack of research on the maneuvering methods, considering the inherent active characteristics of each type of water jet. In this paper, we analyze the sideway method suitable for the condition of Coast Guard Exclusive wharf without assistance, based on the astern performance of each type. Then, a ship handling simulator was used for the experiment; they compared and verified through interviews of captains.

Studies of separation and quantitation for selenium species in food (식품중의 셀레늄 화학종의 분리 및 정량연구)

  • Jang, Hee-Young;Min, Hyungsik;Lee, Jonghae;Pak, Yong-Nam
    • Analytical Science and Technology
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    • v.26 no.3
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    • pp.182-189
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    • 2013
  • The purpose of this research is to separate and quantitate selenium species in some food samples with HPLC-ICP-MS. Cation exchange chromatography showed efficient separation only for inorganic Se species while reversed phase ion pair chromatography showed good separation for both inorganic and organic Se species. $C_8$ column ($Symmetryshield^{TM}\;RP_8$, 3.5 ${\mu}m$, $4.6{\times}150$ mm) was used with optimum condition of 5% methanol mobile phase, 0.05% of nonafluorovaleric acid ion pairing reagent. Five standard Se species of Se(IV), Se(VI), SeCys(selenocystein), SeMet(selenomethionine) and Se-M-C(seleno methyl cystein) were separated successfully under the optimum condition (mobile phase; 5% methanol, ion-pairing reagent; 0.05% nonafluorovaleric acid, flow rate; 0.9 mL $min^{-1}$). To extract Se species, microwave assisted and enzyme-assisted extraction methods were studied. In enzyme-assisted extraction method, protease I for garlic, protease I plus trypsin for pork and mackerel, and protease XIV for tuna showed the best extraction efficiency. With the optimum condition for each sample, it was found that mostly inorganic Se, SeCys and SeMet are present in the sample studied ranging from few ${\mu}g$ $g^{-1}$ to few tens of ${\mu}g$ $g^{-1}$.

Height Control of Pot Chrysanthemum according to Daminozide Applications at Different Planting Period in C-Channel mat Irrigation System (C-형강 매트 저면관수 시스템을 이용한 분화 국화 재배 시 정식시기와 Daminozide 처리에 따른 초장 조절)

  • Kang, Seung Won;Seo, Sang Gyu;Lee, Gung Pyo;Pak, Chun Ho
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.1
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    • pp.30-36
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    • 2011
  • Effect of daminozide concentration and application methods, in combination with different planting period, on the growth of chrysanthemum was investigated for small pot-plant production. Daminozide, a plant growth regulator widely used for plant height control, was applied by drench or sub-application. For pot chrysanthemums of first planting time, plant height was reduced over $4,000mg{\cdot}L^{-1}$, when daminozide was drenched directly into the pot medium. However, $5,000mg{\cdot}L^{-1}$ of daminozide drench affected plant height reduction for secondarily planted pot chrysanthemum. Analysis of variance revealed the plant height was affected by planting time. For sub-application experiment of daminozide, it showed that the daminozide affected the number of flower buds formation and fresh and dry weight. These results suggest that planting period affected growth retardation of chrysanthemum. Therefore, application of growth retardant in combination with planting time and application methods may provide more efficient growth control for pot-chrysanthemum production.

Analysis of Low Molecular Weight of Seleno compounds in Selenium-Fortified Spirulina (셀레늄 강화 스피룰리나에서의 낮은 분자량 셀레노 화합물 분석)

  • Ji, Young;Lee, Jung Suk;Han, Young-Seok;Pak, Yong N.
    • Journal of the Korean Chemical Society
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    • v.63 no.5
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    • pp.335-341
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    • 2019
  • Spirulina was cultured in Selenium solution and the total concentration was determined with isotope dilution technique. Low-molecular-weight-Selenium species for the water extract of Spirulina were separated and quantified with HPLC ICP/MS. Water extraction was used first and then protein enzyme (protease XIV) was used to digest and extract for the Se species in both water extract and residue. The total Se was $414.9{\pm}4.0{\mu}g\;g^{-1}$ and 77% existed in water extract while 22% remained in residue. Se species in supernatant was mostly inorganic selenate ($222.7{\mu}g\;g^{-1}$). After hydrolysis of protein, SeCys ($15.20{\mu}g\;g^{-1}$) and SeMet ($12.13{\mu}g\;g^{-1}$) were found. In residue, SeCys and SeMet were found with little inorganic Se. After protein hydrolysis of residue, more of Selenoamino acids SeCys ($9.35{\mu}g\;g^{-1}$) and SeMet ($18.23{\mu}g\;g^{-1}$) in addition to MeSeCys ($1.5{\mu}g\;g^{-1}$) were found. It is thought that inorganic selenium is mostly adsorbed on the surface of spirulina and can be easily removed by a simple distilled water extraction while most of organo-seleniums are remained in residue.