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Construction and Characterization of a cDNA Library from the Camelina sativa L. as an Alternative Oil-Seed Crop (신 바이오디젤 원료 작물인 Camelina의 cDNA library 제작 및 유전자 특성)

  • Park, Won;Jang, Young-Seok;Ahn, Sung-Ju
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.2
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    • pp.151-158
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    • 2010
  • Camelina sativa L., known as popular names "gold-of-pleasure" or "false flax" is an alternative oilseed crop that can be grown under different climatic and soil conditions. Up to date, however, the genomic information of Camelina has not been studied in detail. Therefore, a cDNA library was constructed and characterized from young leaves. The constructed cDNA library incorporated of 1334 cDNA clones and the size of the insertion fragments average was 736 base pair. We generated a total of 1269 high-quality expressed sequence tags (ESTs) sequences. The result of cluster analysis of EST sequences showed that the number of unigene was 851. According to subsequent analysis, the 476 (55.9%) unigenes were highly homologous to known function genes and the other 375 (44.1%) unigenes were unknown. Remaining 63 (7.4%) unigenes had no homology with any other peptide in NCBI database, indicating that these seemed to be novel genes expressed in leaves of Camelina. The database-matched ESTs were further classified into 17 categories according to their functional annotation. The most abundant of categories were "protein with binding function or cofactor requirement (27%)", "metabolism (11%)", "subcellular localization (11%)", "cellular transport, transport facilities and transport routes (7%)", "energy (6%)", "regulation of metabolism and protein function (6%)". Our result in this study provides an overview of mRNA expression profile and a basal genetic information of Camelina as an oilseed crop.

Fast Delineation of the Depth to Bedrock using the GRM during the Seismic Refaction Survey in Cheongju Granite Area (굴절법 탄성파탐사 현장에서 GRM을 이용한 청주화강암지역 기반암 깊이의 신속한 추정)

  • Lee, Sun-Joong;Kim, Ji-Soo;Lee, Cheol-Hee;Moon, Yoon-Sup
    • Economic and Environmental Geology
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    • v.43 no.6
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    • pp.615-623
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    • 2010
  • Seismic refraction survey is a geophysical method that delineates subsurface velocity structure using direct wave and critically refracted wave. The generalized reciprocal method(GRM) is an inversion technique which uses travel-time data from several forward and reverse shots and which can provide the geometry of irregular inclined refractors and structures underlain by hidden layer such as low velocity zone and thin layer. In this study, a simple Excel-GRM routine was tested for fast mapping of the interface between weathering layer and bedrock during the survey, with employing a pair of forward and reverse shots. This routine was proved to control the maximum dip of approximately $30^{\circ}C$ and maximum velocity contrast of 0.6, based on the panel tests in terms of dipping angle and velocity contrast for the two-layer inclined models. In contrast with conventional operation of five to seven shots with sufficient offset distance and indoor data analysis thereafter, this routine was performed in the field shortly after data acquisition. Depth to the bedrock provided by Excel-GRM, during the field survey for Cheongju granite area, correlates well with the elevation of the surface of soft rock from the drill core and SPS logging data. This cost-effective routine developed for quickly delineating the bedrock surface in the field survey will be readily applicable to mapping of weathering zone in narrow zone with small variation of elevation of bedrock.

Studies of separation and quantitation for selenium species in food (식품중의 셀레늄 화학종의 분리 및 정량연구)

  • Jang, Hee-Young;Min, Hyungsik;Lee, Jonghae;Pak, Yong-Nam
    • Analytical Science and Technology
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    • v.26 no.3
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    • pp.182-189
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    • 2013
  • The purpose of this research is to separate and quantitate selenium species in some food samples with HPLC-ICP-MS. Cation exchange chromatography showed efficient separation only for inorganic Se species while reversed phase ion pair chromatography showed good separation for both inorganic and organic Se species. $C_8$ column ($Symmetryshield^{TM}\;RP_8$, 3.5 ${\mu}m$, $4.6{\times}150$ mm) was used with optimum condition of 5% methanol mobile phase, 0.05% of nonafluorovaleric acid ion pairing reagent. Five standard Se species of Se(IV), Se(VI), SeCys(selenocystein), SeMet(selenomethionine) and Se-M-C(seleno methyl cystein) were separated successfully under the optimum condition (mobile phase; 5% methanol, ion-pairing reagent; 0.05% nonafluorovaleric acid, flow rate; 0.9 mL $min^{-1}$). To extract Se species, microwave assisted and enzyme-assisted extraction methods were studied. In enzyme-assisted extraction method, protease I for garlic, protease I plus trypsin for pork and mackerel, and protease XIV for tuna showed the best extraction efficiency. With the optimum condition for each sample, it was found that mostly inorganic Se, SeCys and SeMet are present in the sample studied ranging from few ${\mu}g$ $g^{-1}$ to few tens of ${\mu}g$ $g^{-1}$.

Cytogenetic Analysis of Spotty Belly Greenling (Hexagrammos agrammus) and Greenling (H. otakii) (노래미 (Hexagrammos agrammus)와 쥐노래미 (H. otakii)의 세포유전학적 연구)

  • Sim Mi A;Noh Jae Koo;Nam Yoon Kwon;Kim Dong Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.6
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    • pp.682-685
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    • 2002
  • Cytogenetic analysis was conducted to obtaining informations for genetic improvement of spotty belly greenling (Hexagrmmos agrammus) and greenling (H. otakii) in aquaculture. Erythrocytes of spotty belly greenling were slightly larger than those of greenling (p<0.05). The nuclear volume of spotty belly greening erythrocytes averaged 15.14 $\pm$ 0.92 ${\mu}m^3$ while that of greening averaged 14.61 $\pm$ 0.15 $\mu$m^3 the difference was not significant (p>0.05). Consequently, genome size of spotty belly greenling was also slightly larger than those of greenling. DNA content per cell of spotty belly greenling and greenling were 2.15 pg and 2.10 pg, respectively. The modal chromosome number of both greenling species were same as 2n=48 and karyotypes were also identical as 2 metacentrics, 11 snbrnetacentrics and 11 acrocentric pairs $(W: 74), There was no evidence of polymorphism including aneuploidy or sex-related heterornorphisrn for all specimens examined. The nuclear organizer regions (NOR_s)$ were localized on a small acrocentric chromosome pair in both species, Spotty belly greenling showed large sizes of active rRNA coding regions in their chromosomes. However, greenling examined only small sizes of active rRNA coding regions with dimorphism.

Generation of Feature Map for Improving Localization of Mobile Robot based on Stereo Camera (스테레오 카메라 기반 모바일 로봇의 위치 추정 향상을 위한 특징맵 생성)

  • Kim, Eun-Kyeong;Kim, Sung-Shin
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.13 no.1
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    • pp.58-63
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    • 2020
  • This paper proposes the method for improving the localization accuracy of the mobile robot based on the stereo camera. To restore the position information from stereo images obtained by the stereo camera, the corresponding point which corresponds to one pixel on the left image should be found on the right image. For this, there is the general method to search for corresponding point by calculating the similarity of pixel with pixels on the epipolar line. However, there are some disadvantages because all pixels on the epipolar line should be calculated and the similarity is calculated by only pixel value like RGB color space. To make up for this weak point, this paper implements the method to search for the corresponding point simply by calculating the gap of x-coordinate when the feature points, which are extracted by feature extraction and matched by feature matching method, are a pair and located on the same y-coordinate on the left/right image. In addition, the proposed method tries to preserve the number of feature points as much as possible by finding the corresponding points through the conventional algorithm in case of unmatched features. Because the number of the feature points has effect on the accuracy of the localization. The position of the mobile robot is compensated based on 3-D coordinates of the features which are restored by the feature points and corresponding points. As experimental results, by the proposed method, the number of the feature points are increased for compensating the position and the position of the mobile robot can be compensated more than only feature extraction.

The Change of the Oral Health Status after Applying the Dental Health Education Program for International Marriage Migrant Women (결혼이주여성의 구강보건교육 프로그램 적용 후 구강상태의 변화)

  • Choi, Mi-Sook
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.1
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    • pp.206-213
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    • 2013
  • This study aims at finding which change there is of oral health promotion as investigating the oral health status in quality, diagnosing which effects knowledge and attitude of oral health has before and after the oral health education as developing and conducting the oral health education program for international marriage migrant women to form their oral health belief for improving oral health. This study consisted of each 51 of the experimental group and the controlled group at the multi-cultural family support center from $26^{th}$ of March, 2012 to $30^{th}$ of June, 2012 as the subjects, and pre-to-post investigated knowledge of oral and cognition. Also as it took follow-up examination of the clients who visited to the dentist with changing of their cognition, conducted matched-pair sample t-test and analysis of repeated measure variance. As the result, there were always the changes at the field of knowledge about oral, periodontal disease and toothbrush in awareness of oral health, and at the field of periodontal disease, dental caries, toothbrush, fluorine and bad breath in knowledge of oral health. The change of DMFT index, DT index has been reduced and FT index has been increased. As the result above, the oral health education program for international marriage migrant women has led to change awareness of oral health and knowledge, and the change of knowledge has influenced to a behavior, so there were the changes of periodontal status and DMFT index as well. This has been showing the importance of the program for oral health of international marriage migrant women. Moreover, while the oral health education program is developed in various aspects by offering the information for developing the oral health education program in future, it needs to run parallel prevention with treatment.

EFFECTS OF EUGENOL, CAPSAICIN AND DEMETHOXY-NE ON THE PAIN RESPONSE OF DENTAL PULP (Eugenol, Capsaicin과 Demethoxy - NE가 치수동통 반응에 미치는 영향)

  • Lee, Eun-Goo;Lee, Chung-Sik
    • Restorative Dentistry and Endodontics
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    • v.17 no.1
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    • pp.10-21
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    • 1992
  • The purpose of this study was to investigate the analgesic effect of eugenol, capsaicin and demethoxy-NE. Young adult cats, weighing 2.0 to 3.0kg, were used. Each animal was anesthetized (${\alpha}$-chloralose 60mg per kg body weight) and divided into four groups; control, eugenol, capsaicin and demethoxy-NE group. The anterior digastric muscles were exposed and a pair of electrodes was inserted to record the electromyograms. To expose the pulp, each canine teeth was prepared with a low speed bur under cooling and used for recording anterior digastric muscular EMGs evoked by noxious stimulation of dental pulp. To observe effects on jaw opening reflex, inferior alveolar nerve of both sides were exposed for drug application and wire electrodes were inserted in anterior digstric muscle for recording the EMGs. To observe effects on action potential, saphenous nerves of both sides were exposed and three tissue pools were made from surrounding tissue. The most distal pool was used for applying stimulation, the most proximal one for recording of action potential, and the other one for drug application. One side of inferior alveolar nerve and saphenous nerve were used for eugenol, capsaicin, or demethoxy-NE application, the other side of nerve for control experiments(only vehicle application). Anterior digastric muscular EMGs evoked by noxious stimulation of dental pulp were recorded before drug application, immediate after drug application, at 60 and 120 minutes, and 5 days after drug application. Action potentials were recorded before drug application, immediate after 30 minutes drug application, at 30, 60 and 120 minutes after drug had been washed out. The results were as follows; 1. Eugenol had a continuous blocking effect on the anterior digastric muscular EMGs evoked by noxious pulp stimulation and after 5 days, showed completely blocking effect. 2. After 5 days, demethoxy-NE applied to dental pulp had a considerable blocking effect on the jaw opening retlex evoked by noxious stimulation but capsaicin had no significant effect. 3. After 5 days, eugenol group showed the strongest blocking effect among the all experimental groups on the jaw opening reflex evoked by noxious stimulation of dental pulp and capsaicin group showed the weakest blocking effect. 4. Eugenol had a completely blocking effect on the action potential conductivity of peripheral nerve. Capsaicin and demethoxy-NE had the blocking effect on the action potential conductivity of ${\alpha}$-and C-nerve fibers. 5. Capsaicin, demethoxy-NE and eugenol applied to inferior alveolar nerve surppressed the jaw opening reflex evoked by noxious stimulation of dental pulp.

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Design of Divisible Electronic Cash based on Double Hash Chain (이중해쉬체인에 기반한 분할 가능 전자화폐의 설계)

  • 용승림;이은경;이상호
    • Journal of KIISE:Computer Systems and Theory
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    • v.30 no.7_8
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    • pp.408-416
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    • 2003
  • An electronic cash system has to provide the security, to prevent the double spending and to support the divisibility of electronic cash for the easy of use. Divisible electronic cash system allows an electronic cash to be divided into subdivisions. Each subdivision is worth any desired value, but all values must add up to the original cash value. Divisible scheme brings some advantages. It reduces to make the change and also there is no necessity that a customer must withdraw a cash of the desired value whenever transactions occur. In this paper, we present an electronic cash protocol which provides the divisibility based on the double hash chain technique. Electronic cash is constructed in the form of coins. Coins, generated by the double hush chain, have different denominations. The divisibility based on the double hash chain technique. Electronic cash is constructed in the form of coins. Coins, generated by the double hash chain, have different denominations. The divisibility of an electronic cash is satisfied by the payment certificate, which is a pair of bank´s proxy signature received from the bank. When a customer pays the coin of subdivision, the fairness of that coin is certified by a customer´s signing instead of a bank. Although the proposed method does not guarantee user´s anonymity, it generates coins which cannot be forged, and the customer can use an electronic cash conveniently and efficiently with its divisibility.

E-BLP Security Model for Secure Linux System and Its Implementation (안전한 리눅스 시스템을 위한 E-BLP 보안 모델과 구현)

  • Kang, Jung-Min;Shin, Wook;Park, Chun-Gu;Lee, Dong-Ik
    • The KIPS Transactions:PartA
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    • v.8A no.4
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    • pp.391-398
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    • 2001
  • To design and develop secure operating systems, the BLP (Bell-La Padula) model that represents the MLP (Multi-Level Policy) has been widely adopted. However, user\`s security level in the most developed systems based on the BLP model is inherited to a process that is actual subject on behalf of the user, regardless whatever the process behavior is. So, there could be information disclosure threat or modification threat by malicious or unreliable processes even though the user is authorized in the system. These problems can be solved by defining the subject as (user, process) ordered pair and by defining the process reliability. Moreover, when the leveled programs which exist as objects in a disk are executed by a process and have different level from the process level, the security level decision problem occurs. This paper presents an extended BLP (E-BLP) model in which process reliability is considered and solves the security level decision problem. And this model is implemented into the Linux kernel 2.4.7.

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A Study of Uniformity Test in PET/CT (PET/CT 장치의 uniformitly측정에 관한 연구)

  • Kim, Su-Keun;Jung, Hee-Il;Park, Soung-Ock
    • Journal of radiological science and technology
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    • v.29 no.1
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    • pp.13-19
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    • 2006
  • The PET scanner can detect the photon pair arriving from the source in phantom. The number of light photons released by the crystal(scintillator-BGO or LSO). In recent scintillation crystals in block structures were incorporated into full ring systems, and their resulted marked improvement in spatial resolution and increase in a sensitivity to annihilations. The uniformity of the crystal sensitivity is very important to makes correct information of abnormal states in organs. These factors influenced by the dection efficiency of the scintillators. We have study about the uniformity of crystals to the annihilation, And study about the standard deviation to average counts. The relative standard deviation in central detector groups more uniformed than circumferenced detector groups. It is caused detected quanta of gamma ray by the geometrical factors of PET detector. PET cameras are available with different geometric arrangement and several parallel rings oriented in the axial direction. The center groups from 7th to 40th groups are comparatively uniform and sensitive. But at the circumferenced detectors decreased the sensitivity and uniformity.

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