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http://dx.doi.org/10.5657/kfas.2002.35.6.682

Cytogenetic Analysis of Spotty Belly Greenling (Hexagrammos agrammus) and Greenling (H. otakii)  

Sim Mi A (Department of Aquaculture, Pukyong National University)
Noh Jae Koo (Department of Aquaculture, Pukyong National University)
Nam Yoon Kwon (Department of Aquaculture, Pukyong National University)
Kim Dong Soo (Department of Aquaculture, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.35, no.6, 2002 , pp. 682-685 More about this Journal
Abstract
Cytogenetic analysis was conducted to obtaining informations for genetic improvement of spotty belly greenling (Hexagrmmos agrammus) and greenling (H. otakii) in aquaculture. Erythrocytes of spotty belly greenling were slightly larger than those of greenling (p<0.05). The nuclear volume of spotty belly greening erythrocytes averaged 15.14 $\pm$ 0.92 ${\mu}m^3$ while that of greening averaged 14.61 $\pm$ 0.15 $\mu$m^3 the difference was not significant (p>0.05). Consequently, genome size of spotty belly greenling was also slightly larger than those of greenling. DNA content per cell of spotty belly greenling and greenling were 2.15 pg and 2.10 pg, respectively. The modal chromosome number of both greenling species were same as 2n=48 and karyotypes were also identical as 2 metacentrics, 11 snbrnetacentrics and 11 acrocentric pairs $(W: 74), There was no evidence of polymorphism including aneuploidy or sex-related heterornorphisrn for all specimens examined. The nuclear organizer regions (NOR_s)$ were localized on a small acrocentric chromosome pair in both species, Spotty belly greenling showed large sizes of active rRNA coding regions in their chromosomes. However, greenling examined only small sizes of active rRNA coding regions with dimorphism.
Keywords
Cytogenetic analysis; Karyotypes; Nuclear organizer regions; Hexagrammos agrammus; H otakii;
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