• Tuberculosis and Respiratory Diseases
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• 제75권4호
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• pp.140-149
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• 2013

Background: Plasminogen activator inhibitor type 1 (PAI-1), an important regulator of plasminogen activator system which controls degradation of extracellular membrane and progression of tumor cells, and PAI-1 gene polymorphic variants have been known as the prognostic biomarkers of non-small cell lung cancer patients. Recently, experimental in vitro study revealed that transforming growth factor-${\beta}1$ initiated PAI-1 transcription through epithelial growth factor receptor (EGFR) signaling pathway. However, there is little clinical evidence on the association between PAI-1 A15T gene polymorphism and prognosis of Korean population with pulmonary adenocarcinoma and the influence of activating mutation of EGFR kinase domain. Methods: We retrospectively reviewed the medical records of 171 patients who were diagnosed with pulmonary adenocarcinoma and undergone EGFR mutation analysis from 1995 through 2009. Results: In all patients with pulmonary adenocarcinoma, there was no significant association between PAI-1 A15T polymorphic variants and prognosis for overall survival. However, further subgroup analysis showed that the group with AG/AA genotype had a shorter 3-year survival time than the group with GG genotype in patients with EGFR mutant-type pulmonary adenocarcinoma (mean survival time, 24.9 months vs. 32.5 months, respectively; p=0.015). In multivariate analysis of 3-year survival for patients with pulmonary adenocarcinoma harboring mutant-type EGFR, the AG/AA genotype carriers had poorer prognosis than the GG genotype carriers (hazard ratio, 7.729; 95% confidence interval, 1.414-42.250; p=0.018). Conclusion: According to our study of Korean population with pulmonary adenocarcinoma, AG/AA genotype of PAI-1 A15T would be a significant predictor of poor short-term survival in patients with pulmonary adenocarcinoma harboring mutant-type EGFR.

• 한국발생생물학회지:발생과생식
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• 제9권1호
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• pp.43-48
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• 2005

조혈세포의 주요 서식지가 되는 골수기질세포는 줄기세포의 운영을 결정하는 다양한 인자들을 제공한다. 방사선 요법은 항암치료법으로 널리 활용되고 있으나, 조혈세포의 파괴로 인한 부작용이 심각한 문제로서 조혈세포에 의한 혈액 세포가 빠른 시간 내에 회복되는 것이 필수적이다. 본 연구에서는 방사선을 조사했을 때의 줄기세포 서식지를 구성하는 세포인 골수기질세포에서 발현되는 유전자를 탐색하여 그 기능과 조절 및 혈액 형성을 이해하는 기초를 마련하고자 하였다. 방법론적으로는 polymerase chain reaction(PCR) 및 agarose 전기영동 방법을 활용한 differential display를 활용하였으며, 결과로서 여러 후보 유전자가 선별되었으나, plasminogen activator inhibitor-1(PAI-1) 유전자만이 감마선에 의해 유도됨이 반복 확인되었다. PAI-1 유전자 유도의 의미는 향후에 더 연구해야 할 것이다.

• Reproductive and Developmental Biology
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• 제33권4호
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• pp.203-209
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• 2009

The present study was performed to identify changes of plasminogen activator (PA) and plasminogen activator inhibitor (PAI) in porcine oviduct epithelial cells (POECs) during the estrous cycle. POECs obtained from ovary in pre-ovulatory (Pre-Ov), early to mid-luteal stage (Early-mid L) and post-ovulatory stage (Post-Ov). For the examine of PA activity, $1{\times}10^5$ fresh cells of POECs were cultured in DMEM/Ham F-12 containing 10% FBS and 0.2% amphotericin under humidified atmosphere of 5% $CO_2$ in air and $38^{\circ}C$. The urokinase-type PA (uPA) was observed at 7 days of POECs culture. PA activity was measured with culture prolonged of 0, 3, 6, 12 and 24 h after culture of 7 days. The PA activity were high significantly (p<0.05) at 12 h of culture, but PA activity were decreased with culture periods increased. The PA activity in POECs of Post-Ov stage were higher significantly (p<0.05) than that of Early-mid L and Pre-Ov stage. When PAI-1 and PAI-2 were added during the POECs culture, the PA were observed significant low activity (p<0.05). The PA activity and protein expression were decreased by PA inhibitor. This results suggest that PAI-1 and PAI-2 have a suppressive action on change of PA activity during the estrous cycle of pigs. Specifically, this study using PA inhibitor was effect the PA activity and PAI expression in oviduct epithelial cells in pigs.

• Molecular & Cellular Toxicology
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• 제2권3호
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• pp.212-215
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• 2006

Several reports have suggested a possible relationship between blood coagulation factors and schizophrenia. Plasminogen activator inhibitor type 1 (PAI-1) belongs to a serine protease inhibitor family, which regulates fibrinolysis and proteolysis by inhibiting plasminogen activation. The purpose of this study was to investigate the association of polymorphisms of the PAI-1 gene with schizophrenia in Korean population. Two important polymorphisms (-675 4G/5G and -844 G/A) located on promoter region of the PAI-1 gene were analyzed on 178 schizophrenia patients and 226 controls. The genotypic and allelic associations of -675 4G/5G were found significant. Furthermore, haplotype analysis revealed significant result, which suggests that -675 4G/5G polymorphism might confer increased susceptibility for schizophrenia in Korean population.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.265.1-265
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• 1999

No Abstract, See Full Text

• 대한핵의학회:학술대회논문집
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• 대한핵의학회 2003년도 추계학술대회 및 총회
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• pp.20.1-20.1
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• 2003

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.224.1-224
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• 2000

No Abstract, See Full Text

• 자연과학논문집
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• 제8권2호
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• pp.5-7
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• 1996

{X,$X_n$,n$\geq$1}은 독립이고 평균이 영으로 같은 확률분포를 갖는 확률변수 열이고, {$a_ni$, 1$\leq$i$\leq$n,n$\geq$1}은 수열일 때 가중합 $sum_{i=1}^n$$a_{ni}$$X_i$가 0에 확률 1로 수렴할 충분조건을 제시한다.

• 생명과학회지
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• 제22권10호
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• pp.1371-1377
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• 2012

신장섬유화는 내 외부적인 요인들에 의해 발생하며, 그 요인들에 의해 염증이 생기고 지속적인 손상이 일어날 경우 신기능의 상실이 유발된다. 또한 신장섬유화는 세포 외 기질의 과다축적, TGF-${\beta}$나, TNF-${\alpha}$, IL-1과 같은 사이 토카인에 의해 발생하며, TGF-${\beta}$는 신장 섬유화의 과정과 Type I collagen과 fibronectin, PAI-1을 포함한 섬유화 관련 인자들의 발현 유도에 중요한 역할을 한다. 본 연구에서는 TGF-${\beta}$를 처리한 신장섬유화 유도 모델에서 Moringa oleifera Lam 추출물에 대한 섬유화 관련 인자들의 영향을 확인하였다. 실험 결과 TGF-${\beta}$로 유도된 신장 섬유화 세포에서 모링가 추출물이 fibronectin, Type I collagen과 PAI-1의 단백질 및 mRNA 발현을 저해하였으며, 모링가 추출물 중 모링가 뿌리추출물이 가장 영향이 있는 것으로 확인 되었다. 모링가 뿌리추출물이 어떠한 기전을 통하여 섬유화 관련 인자들의 발현을 조절하는지 알아보기 위한 TGF-${\beta}$로 유도된 $T{\beta}RII$ 및 그 하위 기전의 인산화 정도를 확인한 실험에서 모링가 뿌리추출물이 TGF-${\beta}$로 유도된 $T{\beta}RII$과 그 하위기전의 Smad4, ERK의 인산화를 저해하였다. 그러나 TGF-${\beta}$에 의해 유도된 JNK와 p38, PI3K/AKT의 인산화에는 영향이 없었다. 따라서 모링가 뿌리추출물이 TGF-${\beta}$로 유도된 신장 섬유아세포에서 $T{\beta}RII$와 그 하위 기전인 Smad4, ERK를 통해서 Type I collagen 과 fibronectin, PAI-1의 발현을 조절하여 섬유화를 저해 한다는 것을 예상할 수 있다. 결론적으로 모링가 뿌리추출물이 섬유화 치료 및 완화에 좋은 물질이 될 수 있을 것으로 생각된다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6327-6330
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• 2015

Background: Glioblastoma is a highly aggressive and malignant brain tumor. Risk factors are largely unknown however, although several biomarkers have been identified which may support development, angiogenesis and invasion of tumor cells. One of these biomarkers is PAI-1.4G/5G and A-844G are two common polymorphisms in the gene promotor of PAI 1 that may be related to high transcription and expression of this gene. Studies have shown that the prevalence of the 4G and 844G allele is significantly higher in patients with some cancers and genetic disorders. Materials and Methods: We here assessed the association of 4G/5G and A-844G polymorphisms with glioblastoma cancer risk in Iranians in a case-control study. All 71 patients with clinically confirmed and 140 volunteers with no history and symptoms of glioblastoma as control group were screened for 4G/5G and A-844G polymorphisms of PAI-1, using ARMS-PCR. Genotype and allele frequencies of case and control groups were analyzed using the DeFinetti program. Results: Our results showed significant associations between 4G/5G (p=0.01824) and A-844G (p = 0.02012) polymorphisms of the PAI-1 gene with glioblastoma cancer risk in our Iranian population. Conclusions: The results of this study supporting an association of the PAI-1 4G/5G (p=0.01824) and A-844G (p = 0.02012) polymorphisms with increasing glioblastoma cancer risk in Iranian patients.