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http://dx.doi.org/10.5352/JLS.2012.22.10.1371

Anti-Fibrotic Effects by Moringa Root Extract in Rat Kidney Fibroblast  

Park, Su-Hyun (Research Institute of Biomedical Engineering, Catholic University of Daegu School of Medicine)
Chang, Young-Chae (Research Institute of Biomedical Engineering, Catholic University of Daegu School of Medicine)
Publication Information
Journal of Life Science / v.22, no.10, 2012 , pp. 1371-1377 More about this Journal
Abstract
Fibrosis in kidney by internal and external factors causes progressive loss of renal function. Renal fibrosis is the inevitable consequence of an excessive accumulation of the extracellular matrix. TGF-${\beta}$ plays an important role in the process of renal fibrosis and stimulates the synthesis of profibrotic factors, including collagens, fibronectin, and plasminogen activator inhibitor (PAI-1). We examined the effect of Moringa oleifera Lam (moringa) extracts in a rat kidney fibrosis model. We found that moringa root extract suppresses protein expression/mRNA levels of Type I collagen, fibronectin, and PAI-1 induced by TGF-${\beta}$ in renal fibroblasts. Moringa root extract selectively inhibited phosphorylation of TGF-${\beta}$-induced $T{\beta}RII$ and the downstream signaling pathway (e.g., Smad4), and phospho-ERK, but not JNK, p38, or PI3K/AKT. These results suggest that moringa root extract can act against TGF-${\beta}$-induced renal fibrosis in rat kidney fibroblast cells by a mechanism related to its antifibrotic activity, which regulates expression of fibronectin, Type I collagen, and PAI-1 through $T{\beta}RII$-Smad2/3-Smad4 and ERK. Therefore, moringa root extract is an effective substance for fibrosis therapy and provides a new therapeutic strategy for diseases associated with elevated profibrotic factor synthesis.
Keywords
Fibrosis; moringa; PAI-1; Type I collagen; fibronectin;
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