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Distribution of Dominant Bifidobacteria in the Intestinal Microflora of Korean Adults and Seniors, Identified by SDS-PAGE of Whole Cell Proteins and 16S rDNA Sequence Analysis

  • KIM TAE WOON;SONG HEE SUNG;KIM HAE YEONG
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.388-394
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    • 2005
  • In order to investigate the distribution of dominant Bifidobacterium species in intestinal microflora of Korean adults and seniors, SDS-PAGE profiles of whole cell proteins were used for the identification of bifidobacteria. To confirm the reliability of SDS-PAGE, the Bifidobacterium species identified by SDS-PAGE of whole cell proteins were validated by using 16S rDNA sequencing analysis. The results of SDS­PAGE corresponded well with those determined by the analysis of 16S rDNA sequencing. Based on the analysis of SDS-PAGE patterns on unidentified fecal strains which showed positive in fructose-6-phosphate phosphoketolase activity, B. adolescentis, B. longum, and B. bifidum were identified in the feces of adults, and B. adolescentis, B. longum, B. bifidum, B. breve, and B. dentium were identified in those of seniors. In most of the fecal samples tested, the predominant Bifidobacterium species consisted of only a few species, and differences in the distribution and numbers of Bifidobacterium species were observed between adults and seniors. B. adolescentis and B. longum were found to be the most common species in feces of adults, but not in seniors. Accordingly, the distribution and abundance of bifidobacteria in the human intestinal microflora varied depending on the age of hosts.

Web Crawling and PageRank Calculation for Community-Limited Search (커뮤니티 제한 검색을 위한 웹 크롤링 및 PageRank 계산)

  • Kim Gye-Jeong;Kim Min-Soo;Kim Yi-Reun;Whang Kyu-Young
    • Proceedings of the Korean Information Science Society Conference
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    • 2005.07b
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    • pp.1-3
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    • 2005
  • 최근 웹 검색 분야에서는 검색 질을 높이기 위한 기법들이 많이 연구되어 왔으며, 대표적인 연구로는 제한 검색, focused crawling, 웹 클러스터링 등이 있다. 그러나 제한 검색은 검색 범위를 의미적으로 관련된 사이트들로 제한할 수 없으며, focused crawling은 질의 시점에 클러스터링하기 때문에 질의 처리 시간이 오래 걸리고, 웹 클러스터링은 많은 웹 페이지들을 대상으로 클러스터링하기 위한 오버헤드가 크다. 본 논문에서는 검색 범위를 특정 커뮤니티로 제한하여 검색 하는 커뮤니티 제한 검색과 커뮤니티를 구하는 방법으로 cluster crawler를 제안하여 이러한 문제점을 해결한다. 또한, 커뮤니티를 이용하여 PageRank를 2단계로 계산하는 방법을 제안한다. 제안된 방법은 첫 번째 과정에서 커뮤니티 단위로 지역적으로 PageRank를 계산한 후, 두 번째 과정에서 이를 바탕으로 전역적으로 PageRank론 계산한다. 제안된 방법은 Wang에 의해 제안된 방법에 비해 PageRank 근사치의 오차를 $59\%$ 정도로 줄일 수 있다.

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Component proteins in cystic fluid of Taenia sodium metacestodes collected surgically from neurocysticercosis patients (인체 유구낭미충증 환자에서 수거한 낭미충 낭액의 성분 단백질의 양상)

  • Yoon Kong;Shin-Yong Kang;Seung-Yull Cho
    • Parasites, Hosts and Diseases
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    • v.28 no.2
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    • pp.101-108
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    • 1990
  • Surgically collected cystic fluid of Taenia solium metacestodes from patients of intracranial cystic lesion were compared in their protein composition with those from naturally infected pigs in Cheju Do, Korea and Ecuador. In non-denaturing discontinuous-polyacryla aide gel electrophoresis (disc-PAGE) , no discernible differences were recognized in banding patterns between the cystic fluids from Cheju Do and Ecuador, and between the cystic quids from pigs and human lesions except wider bands that corresponded to human albumin and T-globulin (in 4 of 9 patients). In reducing SDS-PAGE, bands in the cystic Ruid from Ecuador showed the same banding pattern with that from Cheju Do but two bands of 21 and 17 kDa were stained darker. Cystic quids (rom patients revealed the same protein compositions of the major protein bands of 94, 64, 15, 10 and 7 kDa as in the cystic fluid of pig origin, but human albumin (66 kDa), heavy and light chains of gamma globulin (55 and 22.5 kDa) were contaminated in 4 of 9 cystic fluids. Human CSF proteins seem to have been contaminated during cystic ftuid collection. In any cystic quid from patients, the majcr Protein component was 150 kDa which was subdivided into 15, 10 and 7 kDa in reducing SDS-PAGE.

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A Recovery Technique of PDF File in the Unit of Page (PDF 파일의 페이지단위 복구 기법)

  • Jang, Jeewon;Bang, Seung Gyu;Han, Jaehyeok;Lee, Sang Jin
    • KIPS Transactions on Computer and Communication Systems
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    • v.6 no.1
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    • pp.25-30
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    • 2017
  • The influence of the data deletion method which is one of anti-forensic techniques is substantial in terms of forensic analysis compared to its simplicity of the act. In academic world, recovery techniques on deleted files have been continuously studied in response to the data deletion method and representatively, the file system-based file recovery technique and file format based recovery technique exist. If there's metadata of deleted file in file system, the file can be easily recovered by using it, but if there's no metadata, the file is recovered by using the signature-based carving technique or the file format based recovery technique has to be applied. At this time, in the file format based recovery technique, the file structure analysis and possible recovery technique should be provided. This paper proposes the page recovery technique on deleted PDF file based on the structural characteristics of PDF file. This technique uses the tag value of page object which constitutes one page of PDF file. Object is extracted by utilizing each tag value as a kind of signature and by analyzing extracted object, the metadata of PDF file is recombined and then it's reconfigured page by page. Recovering by page means that even if deleted PDF file is damaged, even some pages consisting of PDF file can be recovered. Generally, if the file system based file is not recoverable, deleted file is recovered by applying the signature based carving technique. The technique which we proposed in this paper can recover PDF files that are damaged. In the digital forensic perspective, it can be utilized to recover more data than previously.

Index Management Method using Page Mapping Log in B+-Tree based on NAND Flash Memory (NAND 플래시 메모리 기반 B+ 트리에서 페이지 매핑 로그를 이용한 색인 관리 기법)

  • Kim, Seon Hwan;Kwak, Jong Wook
    • Journal of the Korea Society of Computer and Information
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    • v.20 no.5
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    • pp.1-12
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    • 2015
  • NAND flash memory has being used for storage systems widely, because it has good features which are low-price, low-power and fast access speed. However, NAND flash memory has an in-place update problem, and therefore it needs FTL(flash translation layer) to run for applications based on hard disk storage. The FTL includes complex functions, such as address mapping, garbage collection, wear leveling and so on. Futhermore, implementation of the FTL on low-power embedded systems is difficult due to its memory requirements and operation overhead. Accordingly, many index data structures for NAND flash memory have being studied for the embedded systems. Overall performances of the index data structures are enhanced by a decreasing of page write counts, whereas it has increased page read counts, as a side effect. Therefore, we propose an index management method using a page mapping log table in $B^+$-Tree based on NAND flash memory to decrease page write counts and not to increase page read counts. The page mapping log table registers page address information of changed index node and then it is exploited when retrieving records. In our experiment, the proposed method reduces the page read counts about 61% at maximum and the page write counts about 31% at maximum, compared to the related studies of index data structures.

Page Logging System for Web Mining Systems (웹마이닝 시스템을 위한 페이지 로깅 시스템)

  • Yun, Seon-Hui;O, Hae-Seok
    • The KIPS Transactions:PartC
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    • v.8C no.6
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    • pp.847-854
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    • 2001
  • The Web continues to grow fast rate in both a large aclae volume of traffic and the size and complexity of Web sites. Along with growth, the complexity of tasks such as Web site design Web server design and of navigating simply through a Web site have increased. An important input to these design tasks is the analysis of how a web site is being used. The is paper proposes a Page logging System(PLS) identifying reliably user sessions required in Web mining system PLS consists of Page Logger acquiring all the page accesses of the user Log processor producing user session from these data, and statements to incorporate a call to page logger applet. Proposed PLS abbreviates several preprocessing tasks which spends a log of time and efforts that must be performed in Web mining systems. In particular, it simplifies the complexity of transaction identification phase through acquiring directly the amount of time a user stays on a page. Also PLS solves local cache hits and proxy IPs that create problems with identifying user sessions from Web sever log.

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A Study on influence factors of User's Continuous Use Intension in Firm Promotion Social Network Service: by Mediating the Moderator of the Operation Objective Types in Facebook Fan Page (기업 SNS 이용자(고객)의 지속적 사용의도에 관한 연구:Facebook 팬페이지 운영 목적을 조절변수로)

  • Shim, Eunsun;Lee, JungHoon;Jeong, BeobGeun
    • Journal of Information Technology Services
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    • v.12 no.4
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    • pp.41-57
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    • 2013
  • As the usage of SNS has grown dramatically over the last years, it has been used in various sectors not just a tool for communication. In particular, corporate companies actively use Facebook as a tool for corporate marketing. Corporate companies communicate with their customers by providing service or events information. In this study, empirical analysis was carried out on corporate companies that are using Facebook as a marketing tool, thus finding out the influencing factors in continued usage of Facebook Fan Page users. In addition, analysis of the factors will be investigated according to continued usage of the users, where the user's intention will differ depending on how corporate companies operate and deal with the Facebook Fan Page. Factors affecting the user's satisfaction, perceived usefulness, and perceived playfulness were deducted through the analysis of the existing literature study on corporate company's continued intention on the usage of Facebook Fan Page. Accordingly, factor affecting this such as relationship, speed, content diversity, which are the media characteristics of the Facebook were deducted and finally hypothesized. In addition, the type of corporate company's Facebook Fan Page was divided into event type, communication type, information service type. And hence investigated the factors that differed from the continued usage depending on the type. Results analysis shows that perceived usefulness affected only relationship, speed, content diversity. Furthermore, analysis shows that corporate company's Fan Page of communication and event types are the factors that affect the usage intention which meant it only affected the perceived enjoyment. On the basis of this study, we are able to present an implication of value where corporate company use it as a marketing tool, through factors from continued usage of coroporate company's Facebook Fan Page users.

Generating Mashup Client View Navigation Codes using REST Style Service Patterns (REST 서비스 패턴을 이용한 매시업 클라이언트 뷰 이동 코드 생성)

  • Lee, Eun-Jung
    • The KIPS Transactions:PartD
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    • v.17D no.5
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    • pp.359-370
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    • 2010
  • As web 2.0 becomes one of the important architecture styles, more web applications adopt single page structure instead of multiple web pages and navigations between pages. A single page web application client, called a mashup client in this paper, interfaces more than one services and allows users to navigate in the page. A mashup client page includes complicated functions and has to handle various styles of services and user requirements, and therefore is usually developed manually. In this paper, we propose a model driven code generation approach for in-page navigations. We propose a page model and view navigation design approach, applying REST service architecture patterns. Then, we consider type conditions for each view to have service calls or navigation controls. Also, we developed an XForms page code generation system to demonstrate the efficiency of the proposed method. The developed system generates mashup client pages including navigation controls between services and views. This system can generate ready to use codes from service specifications, so this can help to reduce the development overhead. Moreover, our approach is based on formal model and navigation patterns so the generated result code is simple and easy to understand, and includes only the necessary controls. Therefore, the proposed approach can be more effective for the case of a large number of services.

Effect of ${\gamma}-irradiation$ on the Physicochemical Properties of Soy Protein Isolate and Whey Protein Concentrate (감마선 조사가 Soy Protein Isolate와 Whey Protein Concentrate의 이화학적 성질에 미치는 영향)

  • Cho, Yong-Sik;Song, Kyung-Bin
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1488-1494
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    • 1999
  • Effect of ${\gamma}-irradiation$ on the SDS-PAGE pattern, secondary structure content, the solubility of commercial soy protein isolate (SPI) and whey protein concentrate (WPC) was investigated. The change in the subunit molecular weight of SPI and WPC irradiated in aqueous solution or dried state was studied using SDS-PAGE. The SDS-PAGE pattern of SPI irradiated in aqueous solution revealed the fragmentation and aggregation of the subunit protein. For WPC irradiated in aqueous solution. fragmentation of the subunit protein up to 10 kGy was observed. In contrast, ${\gamma}-irradiation$ of SPI and WPC in dried state did not cause any significant changes in the SDS-PAGE pattern. The change In the secondary structure of irradiated SPI and WPC solution was studied using circular dichroism. The aperiodic structure content of SPI and WPC solution increased at higher irradiation doses, which suggests that ${\gamma}-irradiation$ caused the disruption of the ordered structure of SPI and WPC solution. Gamma-irradiation also caused the change in solubility of SPI and WPC in dried state.

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Development of a Web Page for Real-time Meteorological Observation Data Service Using AWS (자동기상관측시스템을 활용한 실시간 기상 관측 자료 제공 웹 페이지 개발)

  • Kim, Yong-Nam;Seong, Gi-Hong;Hong, Jeong-Hee;Kang, Dong-Il
    • Journal of the Korean earth science society
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    • v.30 no.4
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    • pp.478-484
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    • 2009
  • A web page was developed to enhance students' learning experience in studying meteorological phenomena. After collecting the meteorological elements observed with automatic weather observation system (AWS), it serve real-time meteorological information on demand. Past meteorological information as well as real-time current information can be retrieved because the web page can save and accumulate observed information in its data base. The completed web page was successfully applied in school settings in teaching students meteorology research sections of earth science. The results show that students experienced authentic and meaningful learning through the real-time meteorological information from the web page. In addition, large scale of time was required to observe meteorological phenomena and it hindered practical meteorological research in earth science classes. However, it is expected that the time limitation can be overcome by utilizing accumulated meteorological information of the web page.