• IMMUNE NETWORK
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• v.12 no.5
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• pp.213-216
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• 2012

Our previous report showed that polyacetylene compound, 1-Heptadecene-11, 13-diyne-8, 9, 10-triol (PA) from the root of Cirsium japonicum var. ussuriense has anti-inflammatory activity. In this study we investigated the role of the PA as inhibitor of caspase-1, which converts prointerleukin-$1{\beta}$ (proIL-$1{\beta}$) to active IL-$1{\beta}$ and is activated by inflammasome involved in the inflammatory process. We tested the effect of PA on the production of pro-inflammatory cytokines, IL-$1{\beta}$ in murine macrophage cell line, RAW264.7. PA inhibited lipopolysaccharide (LPS)-induced IL-$1{\beta}$ production by macrophages at a dose dependent manner. PA also suppressed the activation of caspase-1. The mRNA level of ASC (apoptosis-associated spec-like protein containing a CARD), an important adaptor protein of inflammasome, was decreased in the PA treated group. Therefore our results suggest that the anti-inflammatory effect of PA is due to inhibit the caspase-1 activation.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.403-411
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• 2005

The relationship between plasmid (~9.5 kb) and pediocin PA-1 in P. acidilactici K10 was confirmed by plasmid curing. The pediocin operon of P. acidilactici K10 was amplified by PCR (polymerase chain reaction), and the nucleotide sequence was analyzed. The sequence of the pediocin operon of P. acidilactici K10 was similar to those of P. acidilactici strains producing pediocin PA-1/ AcH. For the expression of pediocin PA-1 in E. coli, a pQEPED (pQE-30 Xa::mature pedA) was constructed. His-tagged recombinant pediocin PA-1 (-6.5 kDa) was translated by cell-free in vitro transcription and translation using pQEPED as a DNA template. Theresult of slot blotting assay showed that transcription of recombinant pedA in E. coli M15 was induced by the addition of isopropyl-$\beta$-D-thiogalactopyranoside (IPTG) at the final concentration of 1 mM. Although the recombinant pediocin PA-1 inhibited the growth of E. coli, it was expressed in the host strain and purified by nickel-nitrilotriacetic acid (Ni-NTA) metal-affinity chromatography under denaturing condition. This is the first report for the production and one-step purification of biologically active recombinant pediocin PA-1 in E. coli.

• Journal of Preventive Medicine and Public Health
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• v.54 no.6
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• pp.461-470
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• 2021

Objectives: The objective of this study was to determine the prevalence of low physical activity (PA) in Peruvian adults and to identify associated factors. Methods: An analytical study was performed using data from the 2017-2018 Nutritional Food Surveillance by Life Stages survey. The outcome variable was low PA (yes or no), assessed using the International Physical Activity Questionnaire-short form. Prevalence ratios were estimated as a measure of association. Results: Among the 1045 persons included in the analysis, the age-standardized prevalence of low PA was 61.9%. The adjusted model showed that being female and migrating from a rural to an urban area in the last 5 years were associated with a higher probability of having low PA than males and individuals who had not migrated, while residing in rural highlands and jungle areas was associated with a reduced probability of having low PA compared to people residing in other geographic domains. Conclusions: Being a female and migration from a rural to an urban area in the last 5 years were associated with a higher likelihood of having low PA. Therefore, promotion and prevention strategies related to PA are required, especially in the female and migrant populations.

• Journal of Biomedical Engineering Research
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• v.39 no.4
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• pp.161-167
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• 2018

Polyvinyl alcohol/sodium carboxymethyl cellulose (PVA/NaCMC) hydrogels were prepared by physical crosslinking (cyclic freezing/thawing) and gamma (${\gamma}$)-ray irradiation to evaluate the effect of NaCMC concentration (2~8 wt%) on the mechanical properties and the biocompatibility of the PVA/NaCMC hydrogels. The swelling rate of PVA/NaCMC hydrogels regardless of irradiation rose with increasing NaCMC content from 2 wt% to 8 wt%, while the gelation rate was the reverse. As the NaCMC content increased from 2 wt% to 6 wt%, the compressive strength of the hydrogels increased dramatically from $8.5{\pm}2.0kPa$ to $52.7{\pm}2.5kPa$ before irradiation and from $13.5{\pm}2.9kPa$ to $65.5{\pm}8.7kPa$ after irradiation. When 8 wt% NaCMC was added afterwards, the compressive strength decreased however. The irradiated PVA/NaCMC hydrogels containing 6 wt% NaCMC exhibited the tailored properties of the swelling rate of $118{\pm}3.7%$, the gelation rate of $71.4{\pm}1.3%$, the strength of $65.5{\pm}8.7kPa$, respectively, and no cytotoxicity was observed.

• Journal of the Korean Institute of Gas
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• v.17 no.5
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• pp.15-21
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• 2013

This study evaluated comparison of the risk according to the type of fuel by three-dimensional simulation tool(FLACS). The consequence analysis of fire explosion and jet-fire was carried out in the layout of a typical high-pressure gas filling stations using CNG, hydrogen and 30%HCNG. Under the same conditions, hydrogen had a 30kPa maximum overpressure, CNG had a 0.4kPa and HCNG had a 3.5kPa. HCNG overpressure was 7.75 times higher than the CNG measurement, but HCNG overpressure was only 11.7% compared to hydrogen. In case of flame propagation, hydrogen had a very fast propagation characteristics. On the other hand, CNG and HCNG flame propagation velocity and distance tended to be relatively safe in comparison to hydrogen. The estimated flame boundary distance by jet-fire of hydrogen was a 5.5m, CNG was a 3.4m and HCNG was a 3.9m.

• Journal of Radiation Industry
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• v.5 no.1
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• pp.69-73
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• 2011

Polyamide 66 (PA66) nanofibers with Triallyl cyanurate (TAC) were obtained by electrospinning of formic acid and chloroform solution. Electron beam irradiation of PA66 nanofiber with and without TAC was carried out over a range of absorbed doses (20~100 kGy) in nitrogen. The characterization of the irradiated PA66 nanofibers and PA66 nanofibers with TAC was done by scanning electron microscopy (SEM), nuclear magnetic resonance (NMR), thermogravimetric analysis (TGA) and universal testing machine (UTM). The results of the SEM image analysis confirmed that the morphology of PA66 nanofibers was not altered by electron beam. The amount of TAC in PA66 nanofiber with TAC was identified by $^1H-NMR$ analysis. The degradation temperature of PA66 nanofibers with TAC at an absorbed dose of 20~100 kGy was higher than the irradiated PA66 nanofiber without TAC. On the other hand, the decreasing rate of modulus of irradiated PA66 nanofibers with TAC was less than PA66 nanofibers.

• Journal of Conservation Science
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• v.28 no.4
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• pp.387-394
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• 2012

Most of the libraries and archives that lacks conservation lab facilities, frequently apply common synthetic resin adhesives such as PVAc group(Pa), acrylic group(Ac) and PVP group(Pv) on simple repairs. The archival quality synthetic adhesive(Pa-1) that is used at the National Library of Korea and the common synthetic resin adhesives were selected for the experiments. Accelerated aging test was applied on the adhesive samples and change of the pH and chromaticity were measured for one month. As the result of the pH mesurments, Pa-1 and Ac-2 were at the acceptable pH range. As the result of the chromaticity mesurments, degree of yellowing(${\Delta}b^*$) showed smaller change to greater change in the following order; Pa-1

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.77-77
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• 2018

천마(Gastrodia elata)는 연중생산을 위해 실내시설 재배 시 생육모델을 구죽하고, 생육단계에 따른 온도, 수분, $CO_2$ 등 환경 조건 설정이 필요하다. 본 연구는 천마의 생육단계 중 괴경형성기와 괴경비대기의 수분함량을 설정하여 최적의 환경조건을 찾기 위해 수행하였다. 먼저 괴경형성기 수분함량 공급은 괴경형성기에 -20kPa, -30kPa, -40kPa로 처리하여 120일간 배양한 뒤, 괴경비대기를 -40kPa로 고정하여 60일간 배양하였다. 반면, 괴경비대기 수분함량 공급은 괴경형성기를 -30kPa로 고정하여 120일간 배양한 뒤 괴경비대기에 -20kPa, -30kPa, -40kPa, -50kPa로 처리하여 60일간 배양하였다. Tensiometer(토양수분장력계)기를 설치하여 수분을 공급하였고, FDR센서 (UbiMas, CoCo sensor, Frequency domain reflectometry type)를 배양토의 깊이 5 cm와 15 cm에 2개를 설치하여 평균값으로 수분함량을 측정하였으며, 전체수량, 성마율, 종마율 등을 조사하였다. FDR센서로 수분함량을 측정한 결과, -20 kPa은 43.3%, -30 kPa은 34.7%, -40 kPa은 29.8%, -50 kPa은 25.3%로 측정되었다. 괴경형성기 수분함량 처리 후 수확기의 상자 당 전체수량은 -30 kPa일 때 985 g으로 가장 많았고, -40 kPa일 때 912 g, -20 kPa일 때 703 g으로 처리간의 유의적인 차이를 보였다. 성마율은 수분함량처리별 각각 25, 34, 30% 이었고, 종마율은 수분함량처리별 각각 53, 73, 65%로 나타났다. 따라서 -30 kPa 처리구가 다른 처리구에 비해 전체수량, 성마율, 종마율 등이 유의적으로 우수하였다. 괴경비대기 수분 함량 처리 후 수확기의 상자 당 전체수량은 -40 kPa일 때 992 g으로 가장 많았고, -50 kPa일 때 955 g, -30 kPa일 때 903 g, -20 kPa일 때 686 g 순으로 나타났다. -30 kPa에서 -50 kPa 사이에서는 전체 수량의 유의성 차이는 없었다. 성마율은 수분함량처리별 각각 20, 30, 35, 33%이었고, 종마율은 수분함량처리별 각각 45, 65, 75, 68%로 나타났다. 따라서 -40 kPa 처리구가 다른 처리구에 비해 전체수량, 성마율, 종마율 등이 유의적으로 우수하였다. 반면 -20 kPa 처리구는 과도한 수분으로 천마가 오히려 부패될 수 있는 환경조건이 조성됨에 따라 성마율, 종마율 등 전체적인 수량 감소에 영향을 미친 것으로 판단되었다.

• Journal of the Korean Vacuum Society
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• v.12 no.1
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• pp.7-15
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• 2003

Spinning Rotor Gauge (SRG) has been used to transfer standard gauge for international comparison at the high vacuum standards. We calibrated a spinning rotor gauge by using dynamic calibration system (DCS) that was a national high vacuum standards system. And its uncertainties were evaluated with the International Organization for Standardization (ISO), they were recognized ai A type uncertainty, B type uncertainty, combined Standard uncertainty, and expanded uncertainty. The combined standard uncertainties were $1.8007\times10^{-5}$ Pa ~ ~$4.8422\times10^{-5}$ Pa for this spinning rotor gauge.

• Proceedings of the IEEK Conference
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• 2003.07b
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• pp.755-758
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• 2003

본 논문에서 단거리전용통신(DSRC)용 OBE에 사용되는 5.8GHz 송신측 ASK와 PA를 one-chip화하여 MMIC로 설계를 및 제작하였다. 설계된 ASK-PA는 3V 단일 공급전원을 사용하였고, 능동 소자로서 GaAs MESFET을 사용하였다. ASK는 회로의 복잡도를 줄이기 위해 직접변조 방식을 채택하였고, 인접채널 간섭의 영향을 줄이기 위하여 드레인 제어 변조회로를 사용하였다. 또한 전력증폭기는 2단으로 하여 AB급으로 동작하도록 전압분배 바이어스회로로 구성하였다. 측정결과 3V의 공급전압에서 전체이득 20.63dB, 송신출력 7.8dBm으로 나타냈다. 공정은 ETRI 0.5㎛ GaAs MESFET 공정을 사용하였고, Chip size는 1.2mm×l.4mm이다.