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http://dx.doi.org/10.4110/in.2012.12.5.213

Polyacetylene Compound from Cirsium japonicum var. ussuriense Inhibited Caspase-1-mediated IL-$1{\beta}$ Expression  

Shim, Hong (Institute of Chronic Disease, Sahmyook University)
Moon, Jung Sun (Traditional Medicines Research Institute, Sahmyook University)
Lee, Sookyeon (Traditional Medicines Research Institute, Sahmyook University)
Yim, Dongsool (Traditional Medicines Research Institute, Sahmyook University)
Kang, Tae Jin (Institute of Chronic Disease, Sahmyook University)
Publication Information
IMMUNE NETWORK / v.12, no.5, 2012 , pp. 213-216 More about this Journal
Abstract
Our previous report showed that polyacetylene compound, 1-Heptadecene-11, 13-diyne-8, 9, 10-triol (PA) from the root of Cirsium japonicum var. ussuriense has anti-inflammatory activity. In this study we investigated the role of the PA as inhibitor of caspase-1, which converts prointerleukin-$1{\beta}$ (proIL-$1{\beta}$) to active IL-$1{\beta}$ and is activated by inflammasome involved in the inflammatory process. We tested the effect of PA on the production of pro-inflammatory cytokines, IL-$1{\beta}$ in murine macrophage cell line, RAW264.7. PA inhibited lipopolysaccharide (LPS)-induced IL-$1{\beta}$ production by macrophages at a dose dependent manner. PA also suppressed the activation of caspase-1. The mRNA level of ASC (apoptosis-associated spec-like protein containing a CARD), an important adaptor protein of inflammasome, was decreased in the PA treated group. Therefore our results suggest that the anti-inflammatory effect of PA is due to inhibit the caspase-1 activation.
Keywords
Polyacetylene compound; Anti-inflammatory activity; Caspase-1; Inflammasome; Cirsium japonicum;
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