• Journal of Microbiology
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• v.35 no.2
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• pp.97-102
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• 1997

Cysteine desulfhydrase (EC 4.4.1.1.) was purified from the culture supernatant of Streptomyces albidoflavus SMF301 by hydroxyapatite, gel filtration and Resource Q ion-exchange chromatography with a purification fold of six identical subunits. The enzyme was stabilized by dithiothreitol and pyridoxal 5'-phosphate during the purification procedures. The optimum pH and temperature were pH 8.6 and 35$^{\circ}C$, respectively. The N-terminal amino acid sequence was identified as A-P-L-P-T-A-D-V-R-S-D-P-G-Y-R-E-W-L-G-E-A-V. The purified cystein desulfhydrase had a high substrate specificity toward cysteine, and exhibited no cystahionine $\gamma$-lyase activity. The $K_m$ value for cysteine was determined to be 0.37 mM.

• Journal of agriculture & life science
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• v.51 no.4
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• pp.97-109
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• 2017

This study was conducted to investigate the effects of anti-inflammatory plant extracts on the in vitro rumen fermentation characteristics and methane emission. Anti-inflammatory plant extracts from Morus bombycis Koidz, Mallotus japonicus L., Morus alba L., Paulownia coreana Uyeki, Isodon japonicus Hara and Ginkgo biloba L. were used in the study. The ruminal fluid(5 mL), McDougall buffer(10 mL), timothy as a substrate(0.3 g) and each anti-inflammatory plant extract(5% of substrate) were dispensed anaerobically into 50mL serum bottle. The mixtures were incubated for 3, 9, 12, 24, 48 and 72h at $39^{\circ}C$ without shaking. Supplementation of the anti-inflammatory plant extracts did not effects characteristics(pH, digestibility of dry matter, glucose concentration, ammonia concentration, protein concentration, VFA) on rumen fermentation. Total gas was showed a different pattern depending on treatments. Carbon dioxide was significantly(p<0.05) higher in Morus alba and Isodon japonicus than in control at 48h. Methane was significantly(p<0.05) lower in treatment than in control at initial fermentation. However the more incubation time was increased, the more methane emission was higher in treatment than in control. The concentrations of polyphenol and flavonoid were higher in Ginkgo biloba. In conclusion, supplementation of the anti-inflammatory plant extracts did not effect on rumen fermentation and methane emission was decreased in initial fermentation.

• The Journal of Engineering Geology
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• v.22 no.4
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• pp.427-437
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• 2012

A total of 100 groundwater samples were collected from the Nonsan area and the behaviors of uranium and radon as natural radionuclides were investigated with respect to other physicochemical components in the groundwater in order to understand their occurrence, properties, and origins. Radionuclide levels were used to construct detailed concentration maps. The concentration of uranium ranges from 0 to 378 ${\mu}g/L$, with an average of 8.57 ${\mu}g/L$, standard deviation of 42.88 ${\mu}g/L$, and median of 0.56 ${\mu}g/L$. The correlation coefficient between uranium and radon is 0.42, whereas these radionuclides show no relation with other physicochemical components in groundwater. It is noteworthy that the uranium level in most samples (97% of the samples) is less than 30 ${\mu}g/L$, where the bedrock of the aquifer is granite or complex rocks located along the boundary between granite and metamorphic rocks. In the Okcheon metamorphic belt, the uranium concentration of most groundwater is less than 1 ${\mu}g/L$. Radon levels varies from 128 to 9,140 pCi/L, with an average of 2,186 pCi/L, standard deviation of 1,725 pCi/L, and median of 1,805 pCi/L. High radon levels (> 4,000 pCi/L) are most common in regions of Jurassic granite, whereas low radon areas are found in regions of sedimentary rock. In conclusion, the distribution and occurrence of radionuclides are intimately related to the basic geological characteristics of the rocks in which the radiogenic minerals are primarily contained.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.178-184
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• 1992

Five varieties of silage corn were cultivated to compare the growth characteristics and productivity. Corn varieties were Pioneer 3144(P 3144), 3160(P 3160), 3282(P 3282), 3352(P 3352) and Suweon 19 (S 19). The corns were seeded on May 2 and harvested on August 15, and measured the plant height, leaf length, leaf width, stem diameter, and yields of fresh and dry matter. The results obtained were as follows: 1. Plant height of P 3352 and P 3144 grown about 280cm throughout the experimental period was the tallest(P<.Ol), and those of P 3160, P 3282 and Suweon 19 ranged from 246.3 to 256.0 cm. 2. Leaf length is the longest in P 3352 and the shortest in P 3282(97.63cm vs 84.17cm), and there was significant(P<.Ol) difference between corn varieties. 3. Stem diameter was the longest in P 3144 and the shortest(P<.Ol) in P 3352(3.28cm vs 2.66cm). 4. P 3144, P 3282 and P 3352 had the greatest(P<.Ol) fresh matter yields per 10a in leaf, stem, and ear and whole plant, respectively. 5. Dry matter yield of P 3282 was the greatest(P<.Ol) in stem(1,080.6 kg/lOa) and leaf(304.5 kg/lOa), and that of P 3352 was the greatest in ear(864.1 kg/lOa) and whole plant yield(2,021 kg/lOa). 6. Ratios of respective part of corn to total dry matter were ranged from 12.7 to 17.8 76 for leaf, 44.5 to 66.9% for stem and husks, and 16.9 to 42.8 % for ear, and the highest in P 3160, P 3160 and P 3352, respectively. Consequently, P 3352, P 3144 and P 3282 shown high productivity in dry matter yield could be recommended as a reliable corn varieties for silage.

• Food Science and Preservation
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• v.14 no.1
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• pp.78-86
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• 2007

This study prepared extracts of Angelica dahurica leaves using reflux water extraction (RW), reflux ethanol extraction (RE) and pressure heating water extraction (PW). The extracts were extraction for levels of polyphenol compounds, antioxidant activities, and inhibitory potencies for xanthine oxidase and tyrosinase. The PW extraction method yielded the highest content of polyphenol compounds (95.23 mg/g). The electron donating abilities (EDAs) of RE and PW extracts were 76.02% and 70.08% respectively. The superoxide dismutase (SOD)-like activities were 13.45 19.00%, when extracts were assayed at 1 mg/mL. The nitrite scavenging ability (pH 1.2) of the PW extract was 54.33% higher than levels shown (44.24%) by the RE and RW extracts. The inhibition of xanthine oxidase by the RW extract was highest (99.71% at 5 mg/mL) while that of the PW extract was over 97% at 500 g/mL. Tyrosinase inhibition was highest in the RE extract (46.25% at 5 mg/mL). All extracts showed dose-dependent inhibitory activities. The results indicated that the PW extract had the highest polyphenol content, the RW and RE extracts had the best nitrite scavenging ability, and the RE extract showed the most pronounced effect on EDA, SOD-like activity and tyrosinase inhibition.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.6
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• pp.434-441
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• 2009

The effect of varying sulfate concentration on continuous fermentative hydrogen production was studied using enriched mixed microflora in continuously fed reactor. Glucose was used as a model substrate for carbohydrates, and hydraulic retention time (HRT) was maintained at 1, 0.5, 0.25 day, respectively. Sulfate concentration was 0${\sim}$20,000 mg/L and the operating pH was maintained at 5.5. The experimental results indicate that hydrogen production is not affected by high sulfate concentration and shorter HRT of 0.25 day enhance hydrogen production. At HRT 1, 0.5, 0.25 day, the hydrogen production rate and hydrogen yield were 2.6, 4.6, 9.4 L/day, and 2.0, 1.8, 1.6 mol $H_2$/mol glucose, respectively. Residual sulfate content was 96${\sim}$98, 95${\sim}$97, and 94${\sim}$97% at HRT 1, 0.5, 0.25 day which show that no sulfate reduction occurred in the reactor during the experiments. Results of Fluorescence In Situ Hybridization (FISH) may indicate the presence of HPB (hydrogen producing bacteria) under all experimental conditions. However, SRB (sulfate reducing bacteria) were not found.

• Journal of Wetlands Research
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• v.17 no.4
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• pp.391-401
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• 2015

In this study, divided into small category groups for the residential area it was carried out monitoring for the runoff during precipitation. Based on the results analyzed according to the nonpoint sources Housing leakage characteristics. Analysis of the rainfall runoff and concentration of each type of exclusive detached house with apartments, in the majority of precipitation types runoff concentrations were higher in early. In the case of a difference of two points per runoff rate rainfall it was largely investigation. The average runoff is estimated loadings of BOD $101.1kg/km^2$, SS $232.2kg/km^2$, T-N $18.2kg/km^2$, T-P $2.0kg/km^2$ detached house case, if the apartment was estimated at point BOD $108.82kg/km^2$, SS $329.18kg/km^2$, T-N $57.67kg/km^2$, T-P $4.21kg/km^2$. The average EMCs is BOD BOD 6.6 mg/L, SS 12.8 mg/L, T-N 1.518 mg/L, T-P 0.099 mg/L detached house case, if the apartment was estimated at point BOD 6.3 mg/L, COD 11.2mg/L, SS 14.5 mg/L, T-N 3.1 mg/L, T-P 0.2 mg/L. The initial 30 percentage calculated based on the initial results, the total flow of 30% if the outflow of detached house showed a net percentage difference to T-P 1.04 > T-N 0.97 > BOD 0.90 > SS 0.80. The apartment area showed the percentage difference in the water quality in the order of BOD 1.49 > T-P 1.40 > SS 1.30 > T-N 0.96 per item.

• Korean Journal of Environmental Agriculture
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• v.28 no.3
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• pp.274-280
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• 2009

In order to elucidate the adsorption mechanism of the herbicide, bentazon and its metabolites on soil, their adsorption patterns on soil and six adsorbents were investigated with Freundlich, Langmuir and linear isotherm. Freundlich constants ($K_f$) and maximum adsorption amount($Q^0$) of bentazon on soil was 0.55 and 3.97. Kd and Koc values of it were 0.18 and 18. The all of metabolites used except deisopropylbentazon amounts sorbed on the soil were much higher than bentazon. The most of 8-hydroxybentazon was adsorbed on soil showing $K_f$ = 316.6, $Q^0$ = 3,488, Kd = 29.7 and Koc = 2,970. Bentazon, deisopropylbentazon and 8-hydroxybentazon were shown high affinity to anion exchange regardless of pH and $NH_2$ in low pH range. Reversed phase $C_{18}$ resulted in 100% retention of N-methylbentazon regardless of pH and other metabolites were retained below 40%. The AIBA was strongly adsorbed in silica gel, COOH and cation exchange phase but poor retention was on anion exchange sorbent. 2-Aminobenzoic acid showed an amphipathic nature which had high affinity for COOH and cation exchange phase at pH 7.0 as well as $NH_2$ and anion exchange sorbent at pH 3.0.

• KSBB Journal
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• v.21 no.1 s.96
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• pp.28-33
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• 2006

A sewage was treated using a horizontal flow zeolite-filled reed bed. The sewage from the student dormitory of Changwon National University was fed into the reed bed for 10 minutes every 6 hours at the hydraulic load of $314L/m^2$ day. The filtering height of the reed bed was 100 cm and the zeolite mixture was filled in the reed bed. The mixture consisted of the same volume of two types of zeolite : $0.5{\sim}1mm$ and $1{\sim}3mm$ in diameter. Annual average removal efficiency was SS $88.5%,\;COD_{cr},\;86.1%,\;COD_{Mn}\;81.0%,\;T-N\;48.6%,\;NH_4^+-N\;97.1%$ and T-P 42.8%. T-N of effluent was mostly $NO_3^--N$ and the concentration of $NO_2^--N$ in effluent was lower than 0.1 mg/L. All removal efficiencies did not show a remarkable seasonal change.

• Journal of Environmental Health Sciences
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• v.22 no.3
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• pp.1-7
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• 1996

Polychlorinated Biphenyls(PCBs)의 생물학적 처리가 시도되고 있으며, PCBs를 분해할 수 있는 미생물을 이용할 수 있다. 따라서 본 연구에서는 폐기된 절연유의 생물학적 처리를 위하여 PCBs를 분해하는 균을 분해하였으며, 분해된 균을 이용하여 절연유 내의 Polychlorinated Biphenyls(PCBs) 분해를 회분식 실험에서 연구하였다. 대구의 신천으로부터 유일한 탄소원으로 Biphenyl을 포함하고 있는 고체배지에서 PCBs를 분해할 수 있는 Pseudomonas sp. P2 균주를 분해하였다. PCBs의 용해도를 높이기 위해 사용된 유화제 alkyl aryl ethoxylated phosphate가 200 mg/L에서는 Pseudomonas sp. P2 균주의 성장에 영향을 미치지 않았다. 1000 mg/L의 Biphenyl과 PCBs에 Pseudomonas sp. P2를 접종하여 160시간 배양후에 Biphenyl과 PCBs의 분해가 각각 97.5%, 58.0%였다. Biphenyl 1000 mg/L에서 최대성장율($\mu_{max}$)은 0.34 $day^{-1}$, 0.26 였다. 따라서 염소가 결합되지 않은 Biphenyl는 염소가 결합된 PCBs보다 분해가 빠르게 진행되었다. 또한 Pseudomonas sp. P2는 Biphenyl과 PCBs의 분해로 부터 유도된 황색의 분해대사산물을 확인하였다. 본 연구에서는 Pseudomonas sp. P2 균주가 절연유 내의 PCBs를 분해할 수 있다는 것을 확인하였다.