• 제목/요약/키워드: P43

검색결과 7,651건 처리시간 0.036초

A GENERALIZATION OF A RESULT OF CHOA ON ANALYTIC FUNCTIONS WITH HADAMARD GAPS

  • Stevic Stevo
    • 대한수학회지
    • /
    • 제43권3호
    • /
    • pp.579-591
    • /
    • 2006
  • In this paper we obtain a sufficient and necessary condition for an analytic function f on the unit ball B with Hadamard gaps, that is, for $f(z)\;=\;{\sum}^{\infty}_{k=1}\;P_{nk}(z)$ (the homogeneous polynomial expansion of f) satisfying $n_{k+1}/n_{k}{\ge}{\lambda}>1$ for all $k\;{\in}\;N$, to belong to the weighted Bergman space $$A^p_{\alpha}(B)\;=\;\{f{\mid}{\int}_{B}{\mid}f(z){\mid}^{p}(1-{\mid}z{\mid}^2)^{\alpha}dV(z) < {\infty},\;f{\in}H(B)\}$$. We find a growth estimate for the integral mean $$\({\int}_{{\partial}B}{\mid}f(r{\zeta}){\mid}^pd{\sigma}({\zeta})\)^{1/p}$$, and an estimate for the point evaluations in this class of functions. Similar results on the mixed norm space $H_{p,q,{\alpha}$(B) and weighted Bergman space on polydisc $A^p_{^{\to}_{\alpha}}(U^n)$ are also given.

진해만의 수산생산성지표의 경년변화 (Annual Variations in Fisheries Productivity Index in Jinhae Bay)

  • 강민희;이인철
    • 한국수산과학회지
    • /
    • 제43권5호
    • /
    • pp.547-550
    • /
    • 2010
  • Jinhae Bay is a typical aquaculture farm with active fisheries. However, it has been contaminated by the development of a major city and industrial area, and has therefore diminished in value as a fishing ground. To preserve the continuing productivity of the ocean, we have to estimate fisheries resources. To analyze the fisheries resources in Jinhae Bay, we evaluated the Fisheries Productivity Index (FPI). The FPI is composed of the Total production ($T_p$), Total amount ($T_a$), and Production of edible protein ($E_p$), nitrogen ($N_p$) and phosphorous ($P_p$). To calculate the FPI, we used Annual statistics on cooperative sales of fishery products data from 1979 to 2004 and the Food composition table. The results of the FPI were as follows. $T_p$ ranged from 70,235 tons to 113,556 tons and $T_a$ ranged from 107,004 million won to 373,776 million won. $E_p$ ranged from 8,124 tons to 13,357 tons, $N_p$ ranged from 1,965 tons to 3,273 tons, and $P_p$ ranged from 168 tons to 276 tons. According to the FPI, maximum fisheries productivity occurred in 1994, when $T_a$, $E_p$, $N_p$ and $P_p$ were at their highest values.

Construction of a Shuttle Vector for Protein Secretory Expression in Bacillus subtilis and the Application of the Mannanase Functional Heterologous Expression

  • Guo, Su;Tang, Jia-Jie;Wei, Dong-Zhi;Wei, Wei
    • Journal of Microbiology and Biotechnology
    • /
    • 제24권4호
    • /
    • pp.431-439
    • /
    • 2014
  • We report the construction of two Bacillus subtilis expression vectors, pBNS1/pBNS2. Both vectors are based on the strong promoter P43 and the ampicillin resistance gene expression cassette. Additionally, a fragment with the Shine-Dalgarno sequence and a multiple cloning site (BamHI, SalI, SacI, XhoI, PstI, SphI) were inserted. The coding region for the amyQ (encoding an amylase) signal peptide was fused to the promoter P43 of pBNS1 to construct the secreted expression vector pBNS2. The applicability of vectors was tested by first generating the expression vectors pBNS1-GFP/pBNS2-GFP and then detecting for green fluorescent protein gene expression. Next, the mannanase gene from B. pumilus Nsic-2 was fused to vector pBNS2 and we measured the mannanase activity in the supernatant. The mannanase total enzyme activity was 8.65 U/ml, which was 6 times higher than that of the parent strain. Our work provides a feasible way to achieve an effective transformation system for gene expression in B. subtilis and is the first report to achieve B. pumilus mannanase secretory expression in B. subtilis.

Cellulosimicrobium sp. YB-43의 mannanase B 유전자 클로닝과 특성 분석 (Molecular cloning and characterization of β-mannanase B from Cellulosimicrobium sp. YB-43)

  • 윤기홍
    • 미생물학회지
    • /
    • 제52권3호
    • /
    • pp.336-343
    • /
    • 2016
  • 두 종류의 mannanases를 생산하는 Cellulosimicrobium sp. YB-43로부터 mannanase 유전자를 클로닝하고 그 염기서열을 결정하였다. Mannanase 유전자는 manB로 명명되었으며, 427 아미노 잔기로 구성된 단백질을 코드하는 1,284개 염기로 구성되었다. ManB는 추론된 아미노산 배열에 근거해서 glycosyl hydrolase family 5에 속하는 mannanase와 상동성이 높은 활성영역과 함께 2개의 탄수화물 결합영역을 포함하고 있는 다영역 효소로 확인되었다. Cellulosimicrobium sp. YB-43의 manB 유전자를 함유한 재조합 대장균의 균체 파쇄상등액으로부터 정제된 ManB의 아미노 말단 배열이 QGASAASDG로 결정되었으며 이는 SignalP4.1 server로 그람 음성균을 기준으로 예측된 signal peptide의 결과와 정확하기 일치하였다. 정제된 ManB의 최적 반응조건은 $55^{\circ}C$와 pH 6.5-7.0이며 locust bean gum (LBG), konjac과 guar gum을 가수분해 하였으며, 셀룰로스, 자일란, 전분과 para-nitrophenyl-${\beta}$-mannopyranoside에 대해서는 분해활성이 없었다. ManB의 활성은 $Mg^{2+}$, $K^+$$Na^+$에 의해 약간 저해되었으며 $Cu^{2+}$, $Zn^{2+}$, $Mn^{2+}$과 SDS에 의해서는 크게 저해되었다. 또한 이 효소는 mannobiose 보다 큰 중합도를 갖는 만노올리고당을 가수분해하였으며, LBG와 만노올리고당을 가수분해하였을 때 mannobiose가 가장 많은 양으로 생성되었다.

Effects of heme oxygenase-1 upregulation on isoproterenol-induced myocardial infarction

  • Eltobshy, Somaia A.G.;Hussein, Abdelaziz M.;Elmileegy, Asaad A.;Askar, Mona H.;Khater, Yomna;Metias, Emile F.;Helal, Ghada M.
    • The Korean Journal of Physiology and Pharmacology
    • /
    • 제23권3호
    • /
    • pp.203-217
    • /
    • 2019
  • The present study was designed to examine the effect of heme oxygenase-1 (HO-1) induction by cobalt protoporphyrin (CoPP) on the cardiac functions and morphology, electrocardiogram (ECG) changes, myocardial antioxidants (superoxide dismutase [SOD] and glutathione [GSH]), and expression of heat shock protein (Hsp) 70 and connexin 43 (Cx-43) in myocardial muscles in isoproterenol (ISO) induced myocardial infarction (MI). Thirty two adult male Sprague Dawely rats were divided into 4 groups (each 8 rats): normal control (NC) group, ISO group: received ISO at dose of 150 mg/kg body weight intraperitoneally (i.p.) for 2 successive days; ISO + Trizma group: received (ISO) and Trizma (solvent of CoPP) at dose of 5 mg/kg i.p. injection 2 days before injection of ISO, with ISO at day 0 and at day 2 after ISO injections; and ISO + CoPP group: received ISO and CoPP at a dose of 5 mg/kg dissolved in Trizma i.p. injection as Trizma. We found that, administration of ISO caused significant increase in heart rate, corrected QT interval, ST segment, cardiac enzymes (lactate dehydrogenase, creatine kinase-muscle/brain), cardiac HO-1, Hsp70 with significant attenuation in myocardial GSH, SOD, and Cx-43. On the other hand, administration of CoPP caused significant improvement in ECG parameters, cardiac enzymes, cardiac morphology; antioxidants induced by ISO with significant increase in HO-1, Cx-43, and Hsp70 expression in myocardium. In conclusions, we concluded that induction of HO-1 by CoPP ameliorates ISO-induced myocardial injury, which might be due to up-regulation of Hsp70 and gap junction protein (Cx-43).

Bacillus spp. 및 Serratia marcescens에 의한 사과 푸른곰팡이병의 생물적 방제 (Biological Control of Blue Mold of Apples by Bacillus spp. and Serratia marcescens)

  • 김용기;이승돈;류재기;류재당
    • 식물병연구
    • /
    • 제9권4호
    • /
    • pp.229-236
    • /
    • 2003
  • 사과 푸른곰팡이병을 방제하기 위하여 사과, 배, 복숭아 등 370점의 과일을 채취하여 과일표면으로부터 세균 1,080균주를 분리하였다. 분리한 1,080균주를 공시 하여 사과 저장병을 일으키는 Penicillium expansum, Alternaria alternata 그리고 Botrytis cinerea을 대상으로 항균활성 및 병 진전억제효과를 검정하여 8종의 길항세균을 선발하였다. 이 들 길항세균에 대한 세균학적 특성과 Biolog system에 의한 영양원 이용성을 조사한 결과 Bacillus amyloliquefaciens가 2균주, B. megaterium, B. subtilis var. gladioli, B. licheniformis, B. pumilus 및 Serratia marcescens로 동정하였다. Glucose, mannose, starch 등 8종의 탄소원을 공시하여 병원균의 생장 및 길항균의 중식에 미치는 영향을 조사한 결과, 병원균의 증식을 억제하고 길항균의 증식을 촉진하는 영양원으로 glucose가 선발되었다. 길항균의 처리효과를 높이기 위하여 길항균 현탁액에 glucose를 1% 첨가하여 사과에 처리한 다음 푸른곰팡이 병균을 $10^5$포자/ml 농도로 처리하였을 때, 병 진전억제효과가 S. marsecens P76-9처리에서 48%, B. amyloliquefaciens P43-2 처리에서 46% 그리고 B. licheniformis P94-1 처리에서 14% 증가되었다. 사과표면의 상처부위에서 처리한 길항균의 밀도변동에 있어서는 Bacillus 속 세균인 B. amyloliquefaciens P43-2과 B. licheniformis P94-1는 처리 후 7일까지 밀도가 증가하다가 처리 후 10일부터는 감소되는 경향이었으며, S. marsecens P76-9는 초기(처리 후 4일)에는 밀도가 감소되다가 처리 후 7일부터 증가되어 처리 후 7일까지도 일정한 수준으로 밀도가 유지되었다.