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http://dx.doi.org/10.5423/RPD.2003.9.4.229

Biological Control of Blue Mold of Apples by Bacillus spp. and Serratia marcescens  

Kim, Yong-Ki (Plant Pathology Division, National Institute of Agricultural Science and Technology)
Lee, Seong-Don (Plant Pathology Division, National Institute of Agricultural Science and Technology)
Ryu, Jae-Gee (Research Coordination Division, Research Management Bureau)
Ryu, Jae-Dang (Plant Pathology Division, National Institute of Agricultural Science and Technology)
Publication Information
Research in Plant Disease / v.9, no.4, 2003 , pp. 229-236 More about this Journal
Abstract
The 1080 epiphytic bacteria obtained from 370 samples of pome and stone fruits including apple, pear, peach, grape, apricot and Chinese quince were screened for antagonistic activity against postharvest pathogens, Penicillium expansum, Alternaria alternata and Botrytis cinerea. Among tested antagonistic bacteria, eight bacterial isolates inhibited mycelial growth of the postharvest pathogens and were identified as Bacillus amyloliquefaciens (three strains), B. megaterium, B. subtilis var. gladioli, B. licheniformis, B. pumilus and Serratia marcescens based on biochemical characteristics and utility of carbon and nitrogen compounds (Biolog system). Eight carbohydrates were evaluated for their effect on mycelial growth and germination of the postharvest pathogen, P. expansum to select nutrients for enhancing bio-control efficacy. The growth of four selected antagonists, B. amyloliquefaciens P43-2, B. amyloliquefaciens A71-2, B. licheniformis P94-1, and S. marcescens P76-9 were also tested. As a result, 1% glucose (w/v) strongly stimulated growth of the antagonists, suppressed mycelial growth of the postharvest pathogen, and had a little comparatively stimulatory effect on germination of the the postharvest pathogen. It was confirmed that the addition of 1% glucose (w/v) greatly enhanced biocontrol effect of B. amyloliquefaciens P43-2, B. licheniformis P94-1, and S. marcescens P76-9. Application of B. amyloliquefaciens P43-2, B. licheniformis P94-1, and S. marcescens P76-9 with the addition of 1% glucose (w/v) increased the control efficacy up to 48%, 46%, 14% compared with those of the antagonists without glucose, respectively. When the antagonists were applied to control postharvest disease caused by P. expansum in apple wounds, the population of B. amyloliquefaciens P43-2 and B. licheniformis P94-1 increased until 4 days after inoculation (DAI) of the antagonists and then decreased from 10 DAI. Meanwhile the population of S. marcescens P76-9 decreased at early stage (4 DAI), but increased from 7 DAI, and finally maintained constantly until 10 DAI in apple wounds.
Keywords
Postharvest disease; Biological control; Bacillus amyloliquefaciens; Serratia marcescens; B. licheniformis; Penicillium expansum; glucose;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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